LOCUS       sequence-39389-e         728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Weinman EJ, Wang Y, Wang F, Greer C, Steplock D, Shenolikar S
  TITLE     A C-terminal PDZ motif in NHE3 binds NHERF-1 and enhances cAMP 
            inhibition of sodium-hydrogen exchange.
  JOURNAL   Biochemistry. 2003 Nov 4. 42(43):12662-8.
  PUBMED    14580213
REFERENCE   2  (bases 1 to 728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2003 Nov 4. 42(43):12662-8."
COMMENT     Sequence Label: pcDNA-NHERF1
FEATURES             Location/Qualifiers
     source          1..728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             97..119
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             130..147
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             157..174
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             181..225
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             241..255
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 tagcagactc tctggctact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctaga aataattttg tttaacttta agaaggagat
      121 atacatatgc accatcatca tcatcattct tctggtctgg tgccacgcgg ttctggtatg
      181 aaagaaaccg ctgctgctaa attcgaacgc cagcacatgg acagcccaga tctgggtacc
      241 gacgacgacg acaaggccat ggctgatcgc gccgctgagc cgtcgcgggg cgagatgagc
      301 gcggacgcgg cggccggggc gcccctgccc cggctctgct gcctggagaa ggggccgaac
      361 ggctacggct tccacctgca tggggagaag ggcaaggtgg gccagtacat ccggctggtg
      421 gagcccggct ctccggccga gaaggcgggg ctgctggccg gggaccggct ggtggaggtg
      481 aacggcgaga acgtggagaa ggagacccac cagcaggtgg tgagccgcat ccgcgccgcg
      541 ctcaacgccg tgcgcctgct ggtggtcgac cccgacacgg acgagcagtt caggaagctc
      601 ggagtccaga tccgggggga gctgctacgc gcccaggcgg ggccggagca ggccgggccg
      661 ccggccgccc ccggcgagca gggcccggcc ggtgagaacg agccgcgcga ggtcgagaag
      721 agccaccc
//
