LOCUS       sequence-39393-e         697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Weinman EJ, Wang Y, Wang F, Greer C, Steplock D, Shenolikar S
  TITLE     A C-terminal PDZ motif in NHE3 binds NHERF-1 and enhances cAMP 
            inhibition of sodium-hydrogen exchange.
  JOURNAL   Biochemistry. 2003 Nov 4. 42(43):12662-8.
  PUBMED    14580213
REFERENCE   2  (bases 1 to 697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2003 Nov 4. 42(43):12662-8."
COMMENT     Sequence Label: pcDNA-N1-PDZ2-GAGA
FEATURES             Location/Qualifiers
     source          1..697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     56..75
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        56..74
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             100..122
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             133..150
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             160..177
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             184..228
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             244..258
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 ttagcagagc tctctggcta ctagaagaac ccactgctta ctggcttatc gaaattaata
       61 cgactcacta tagggagacc caagctggct agaaataatt ttgtttaact ttaagaagga
      121 gatatacata tgcaccatca tcatcatcat tcttctggtc tggtgccacg cggttctggt
      181 atgaaagaaa ccgctgctgc taaattcgaa cgccagcaca tggacagccc agatctgggt
      241 accgacgacg acgacaaggc catggctgat cgcgccgctg agccgtcgcg gggcgagatg
      301 agcgcggacg cggcggccgg ggcgcccctg ccccggctct gctgcctgga gaaggggccg
      361 aacggctacg gcttccacct gcatggggag aagggcaagg tgggccagta catccggctg
      421 gtggagcccg gctctccggc cgagaaggcg gggctgctgg ccggggaccg gctggtggag
      481 gtgaacggcg agaacgtgga gaaggagacc caccagcagg tggtgagccg catccgcgcc
      541 gcgctcaacg ccgtgcgcct gctggtggtc gaccccgaca cggacgagca gttcaggaag
      601 ctcggagtcc agatccgggg ggagctgcta cgcgcccagg cggggccgga gcaggccggg
      661 ccgccggccg cccccggcga gcagggcccg gccggtg
//
