LOCUS       sequence-39397-e         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For mammalian expression of human MEF2B with an HA  tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Han TH, Prywes R
  TITLE     Regulatory role of MEF2D in serum induction of the c-jun promoter.
  JOURNAL   Mol Cell Biol. 1995 Jun;15(6):2907-15.
  PUBMED    7760790
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "1995-06"; volume: "15(6)"; pages: "2907-15"
COMMENT     Sequence Label: pCGN-MEF2B
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..29
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           98..137
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             167..193
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             173..199
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
       61 tccatagaag acaccgggac cgatccagcc tccgggggat cttggtggcg tgaaactccc
      121 gcacctcttc ggccagcgcc ttgtagaagc gcgtatggct tctagctatc cttatgacgt
      181 gcctgactat gccagcctgg gaggaccttc tagaattcga gctcggtacc ccatggggag
      241 gaaaaaaatc cagatctccc gcatcctgga ccaaaggaat cggcaggtga cgttcaccaa
      301 gcggaagttc gggctgatga agaaggccta tgagctgagc gtgctctgtg actgtgagat
      361 agccctcatc atcttcaaca gcgccaaccg cctcttccag tatgccagca cggacatgga
      421 ccgtgtgctg ctgaagtaca cagagtacag cgagccccac gagagccgca ccaacactga
      481 catcctcgag acgctgaagc ggaggggcat tggcctcgat gggccagagc tggagccgga
      541 tgaagggcct gaggagccag gagagaagtt tcggaggctg gcaggcgaag ggggtgatcc
      601 ggccttgccc cgaccccggc tgtatcctgc agctcctgct atgcccagcc cagatgtggt
      661 atacggggcc ttaccgccac caggctgtga ccccagtggg cttggggaag cactgcccgc
      721 ccagagccgc ccatctccct tccgaccagc agcccccaaa gccgggcccc caggcctggt
      781 gcaccctctc ttctcaccaa gccacctcac cagcaagaca ccacccccac tgtacctgcc
      841 gacggaaggg cggaggtcag acctgcctgg tggcctggct gggccccgag ggggactaaa
      901 cacctccaga agcctctaca gtggcctgca gaacccctgc tccactgcaa ctcccggacc
      961 cccactgggg agcttcccct tcctcc
//
