LOCUS       sequence-394046-        9143 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of human EGFR receptor tyrosine kinase in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9143)
  AUTHORS   Gudernova I, Foldynova-Trantirkova S, Ghannamova BE, Fafilek B, 
            Varecha M, Balek L, Hruba E, Jonatova L, Jelinkova I, Kunova 
            Bosakova M, Trantirek L, Mayer J, Krejci P
  TITLE     One reporter for in-cell activity profiling of majority of protein 
            kinase oncogenes.
  JOURNAL   Elife. 2017 Feb 15;6. pii: e21536. doi: 10.7554/eLife.21536.
  PUBMED    28199182
REFERENCE   2  (bases 1 to 9143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-02-15"; volume: "6. pii: e21536. doi"; pages: " 
            10.7554/eLife.21536"
COMMENT     Sequence Label: pcDNA3.1-EGFR-V5/HIS
FEATURES             Location/Qualifiers
     source          1..9143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(54..73)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        245..624
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        625..828
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     779..799
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     873..892
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        873..891
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(949..1020,1021..2883,2884..2952,2953..4578)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     CDS             4651..4692
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4702..4719
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4742..4759)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4748..4972
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5018..5446
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5105..5124)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5315..5336
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5455..5475)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5460..5789
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5640..5775
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5702..5721
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5856..6650
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5910..5929)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6520..6539
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6826..6947
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6863..6882)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6917..6936
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6996..7012)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6996..7012)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7009..7031)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7020..7036
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7044..7050,7051..7068,7069..7074))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7089..7110
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7227..7244)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7398..7986)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7478..7497)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8157..8948,8949..9017))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9018..9122)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc
       61 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
      121 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg
      181 aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg
      241 cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
      301 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      361 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      421 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      481 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      541 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      601 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
      661 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
      721 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
      781 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact
      841 agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa
      901 gctggctagt taagcttggt accgagctcg gatccagtac ccttcaccat gcgaccctcc
      961 gggacggccg gggcagcgct cctggcgctg ctggctgcgc tctgcccggc gagtcgggct
     1021 ctggaggaaa agaaagtttg ccaaggcacg agtaacaagc tcacgcagtt gggcactttt
     1081 gaagatcatt ttctcagcct ccagaggatg ttcaataact gtgaggtggt ccttgggaat
     1141 ttggaaatta cctatgtgca gaggaattat gatctttcct tcttaaagac catccaggag
     1201 gtggctggtt atgtcctcat tgccctcaac acagtggagc gaattccttt ggaaaacctg
     1261 cagatcatca gaggaaatat gtactacgaa aattcctatg ccttagcagt cttatctaac
     1321 tatgatgcaa ataaaaccgg actgaaggag ctgcccatga gaaatttaca ggaaatcctg
     1381 catggcgccg tgcggttcag caacaaccct gccctgtgca acgtggagag catccagtgg
     1441 cgggacatag tcagcagtga ctttctcagc aacatgtcga tggacttcca gaaccacctg
     1501 ggcagctgcc aaaagtgtga tccaagctgt cccaatggga gctgctgggg tgcaggagag
     1561 gagaactgcc agaaactgac caaaatcatc tgtgcccagc agtgctccgg gcgctgccgt
     1621 ggcaagtccc ccagtgactg ctgccacaac cagtgtgctg caggctgcac aggcccccgg
     1681 gagagcgact gcctggtctg ccgcaaattc cgagacgaag ccacgtgcaa ggacacctgc
     1741 cccccactca tgctctacaa ccccaccacg taccagatgg atgtgaaccc cgagggcaaa
     1801 tacagctttg gtgccacctg cgtgaagaag tgtccccgta attatgtggt gacagatcac
     1861 ggctcgtgcg tccgagcctg tggggccgac agctatgaga tggaggaaga cggcgtccgc
     1921 aagtgtaaga agtgcgaagg gccttgccgc aaagtgtgta acggaatagg tattggtgaa
     1981 tttaaagact cactctccat aaatgctacg aatattaaac acttcaaaaa ctgcacctcc
     2041 atcagtggcg atctccacat cctgccggtg gcatttaggg gtgactcctt cacacatact
     2101 cctcctctgg atccacagga actggatatt ctgaaaaccg taaaggaaat cacagggttt
     2161 ttgctgattc aggcttggcc tgaaaacagg acggacctcc atgcctttga gaacctagaa
     2221 atcatacgcg gcaggaccaa gcaacatggt cagttttctc ttgcagtcgt cagcctgaac
     2281 ataacatcct tgggattacg ctccctcaag gagataagtg atggagatgt gataatttca
     2341 ggaaacaaaa atttgtgcta tgcaaataca ataaactgga aaaaactgtt tgggacctcc
     2401 ggtcagaaaa ccaaaattat aagcaacaga ggtgaaaaca gctgcaaggc cacaggccag
     2461 gtctgccatg ccttgtgctc ccccgagggc tgctggggcc cggagcccag ggactgcgtc
     2521 tcttgccgga atgtcagccg aggcagggaa tgcgtggaca agtgcaacct tctggagggt
     2581 gagccaaggg agtttgtgga gaactctgag tgcatacagt gccacccaga gtgcctgcct
     2641 caggccatga acatcacctg cacaggacgg ggaccagaca actgtatcca gtgtgcccac
     2701 tacattgacg gcccccactg cgtcaagacc tgcccggcag gagtcatggg agaaaacaac
     2761 accctggtct ggaagtacgc agacgccggc catgtgtgcc acctgtgcca tccaaactgc
     2821 acctacggat gcactgggcc aggtcttgaa ggctgtccaa cgaatgggcc taagatcccg
     2881 tccatcgcca ctgggatggt gggggccctc ctcttgctgc tggtggtggc cctggggatc
     2941 ggcctcttca tgcgaaggcg ccacatcgtt cggaagcgca cgctgcggag gctgctgcag
     3001 gagagggagc ttgtggagcc tcttacaccc agtggagaag ctcccaacca agctctcttg
     3061 aggatcttga aggaaactga attcaaaaag atcaaagtgc tgggctccgg tgcgttcggc
     3121 acggtgtata agggactctg gatcccagaa ggtgagaaag ttaaaattcc cgtcgctatc
     3181 aaggaattaa gagaagcaac atctccgaaa gccaacaagg aaatcctcga tgaagcctac
     3241 gtgatggcca gcgtggacaa cccccacgtg tgccgcctgc tgggcatctg cctcacctcc
     3301 accgtgcaac tcatcacgca gctcatgccc ttcggctgcc tcctggacta tgtccgggaa
     3361 cacaaagaca atattggctc ccagtacctg ctcaactggt gtgtgcagat cgcaaagggc
     3421 atgaactact tggaggaccg tcgcttggtg caccgcgacc tggcagccag gaacgtactg
     3481 gtgaaaacac cgcagcatgt caagatcaca gattttgggc tggccaaact gctgggtgcg
     3541 gaagagaaag aataccatgc agaaggaggc aaagtgccta tcaagtggat ggcattggaa
     3601 tcaattttac acagaatcta tacccaccag agtgatgtct ggagctacgg ggtgaccgtt
     3661 tgggagttga tgacctttgg atccaagcca tatgacggaa tccctgccag cgagatctcc
     3721 tccatcctgg agaaaggaga acgcctccct cagccaccca tatgtaccat cgatgtctac
     3781 atgatcatgg tcaagtgctg gatgatagac gcagatagtc gcccaaagtt ccgtgagttg
     3841 atcatcgaat tctccaaaat ggcccgagac ccccagcgct accttgtcat tcagggggat
     3901 gaaagaatgc atttgccaag tcctacagac tccaacttct accgtgccct gatggatgaa
     3961 gaagacatgg acgacgtggt ggatgccgac gagtacctca tcccacagca gggcttcttc
     4021 agcagcccct ccacgtcacg gactcccctc ctgagctctc tgagtgcaac cagcaacaat
     4081 tccaccgtgg cttgcattga tagaaatggg ctgcaaagct gtcccatcaa ggaagacagc
     4141 ttcttgcagc gatacagctc agaccccaca ggcgccttga ctgaggacag catagacgac
     4201 accttcctcc cagtgcctga atacataaac cagtccgttc ccaaaaggcc cgctggctct
     4261 gtgcagaatc ctgtctatca caatcagcct ctgaaccccg cgcccagcag agacccacac
     4321 taccaggacc cccacagcac tgcagtgggc aaccccgagt atctcaacac tgtccagccc
     4381 acctgtgtca acagcacatt cgacagccct gcccactggg cccagaaagg cagccaccaa
     4441 attagcctgg acaaccctga ctaccagcag gacttctttc ccaaggaagc caagccaaat
     4501 ggcatcttta agggctccac agctgaaaat gcagaatacc taagggtcgc gccacaaagc
     4561 agtgaattta ttggagcaaa gggtcaagac aattctgcag atatccagca cagtggcggc
     4621 cgctcgagtc tagagggccc gcggttcgaa ggtaagccta tccctaaccc tctcctcggt
     4681 ctcgattcta cgcgtaccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca
     4741 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     4801 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     4861 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     4921 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     4981 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     5041 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     5101 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     5161 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     5221 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     5281 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     5341 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     5401 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     5461 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     5521 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     5581 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     5641 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     5701 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     5761 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     5821 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     5881 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     5941 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     6001 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     6061 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     6121 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     6181 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     6241 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     6301 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     6361 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     6421 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     6481 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     6541 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     6601 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     6661 ggggttcgcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     6721 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     6781 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     6841 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     6901 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     6961 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     7021 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     7081 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     7141 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     7201 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     7261 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     7321 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     7381 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     7441 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     7501 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     7561 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     7621 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     7681 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     7741 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     7801 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     7861 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     7921 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     7981 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     8041 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     8101 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     8161 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     8221 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     8281 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     8341 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     8401 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     8461 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     8521 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     8581 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     8641 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     8701 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     8761 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     8821 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     8881 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     8941 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     9001 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     9061 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     9121 cgcacatttc cccgaaaagt gcc
//
