LOCUS       sequence-39405-e         649 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of the eGFP reporter gene was not detected in the brain 
            and eye..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui
            SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas DJ, Babyak N, Black 
            SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen 
            Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK,
            Glenn R, Hatakka K, Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S,
            Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J,
            Lu M, McConechy M, McLeod AJ, Milisavljevic M, Mis J, O'Connor K, 
            Palma B, Palmquist DL, Schmouth JF, Swanson MI, Tam B, Ticoll A, 
            Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, 
            Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, 
            Goldowitz D, Wasserman WW, Simpson EM
  TITLE     A regulatory toolbox of MiniPromoters to drive selective expression 
            in the brain.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94.
  PUBMED    20807748
REFERENCE   2  (bases 1 to 649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Sep 21. 107(38):16589-94."
COMMENT     Sequence Label: pEMS1351
FEATURES             Location/Qualifiers
     source          1..649
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctcgcccttg ctcaccatgg tggcggaagt tcctatacct tttgaagaat aggaacttcg
       61 gcgcgccacg cgttgaggaa gaaggaagaa cagcaagaga gggatgttga aagacagaga
      121 tggagacacg aaaatggcct tcctacggag cgaaatccct ctttttcccc attcctgtca
      181 ctcagtcttc gatctctccc tctacatagg catgcagagt gtgggcaatc tctgccgacc
      241 gctggtctcc caaggaaaga aggaaaacac taccgcagtc tcggaggcag acgggctccg
      301 cgctgccgga acccaagcgc agagtgggaa aactagattg ctgggaaatc ttccgaggga
      361 agaggcggcc aggtccgggt ttccggggag agccgcgcac gcagcggcga agggtccccg
      421 aagttcatta ccccgtgact ttgtgcctgt cgctgcgggc tgggtcccct gtaatatctc
      481 cagaagcgct ctgacaggca gccgcgggga ggggaatagg gcgtccctgg cccacttagc
      541 ttttccccca actcctggcg gggtctgacg tcagccatgt gcggggaggg gatgggaaga
      601 gggagggggt taaatgctaa tgatattaat gaacctccaa gcggctccc
//
