LOCUS       sequence-394051-       13692 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses Dendra2-Rpb1 fusion in mammalian cells. Possible to insert
            into the genome using the Piggibac system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13692)
  AUTHORS   Cho WK, Jayanth N, English BP, Inoue T, Andrews JO, Conway W, Grimm 
            JB, Spille JH, Lavis LD, Lionnet T, Cisse II
  TITLE     RNA Polymerase II cluster dynamics predict mRNA output in living 
            cells.
  JOURNAL   Elife. 2016 May 3;5. pii: e13617. doi: 10.7554/eLife.13617.
  PUBMED    27138339
REFERENCE   2  (bases 1 to 13692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-05-3"; volume: "5. pii: e13617. doi"; pages: " 
            10.7554/eLife.13617"
COMMENT     Sequence Label: PB53x EF1-Dendra2-RPB1Amr
FEATURES             Location/Qualifiers
     source          1..13692
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     10..27
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    144..165
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(180..185,186..203,204..210)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    218..234
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     223..245
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     242..258
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     242..258
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   749..783
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    1546..1667
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1557..1576)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1611..1630
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1709..1729
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1789..2583)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLAATGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1900..1919)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2510..2529
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     misc_feature    2592..3164
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2751..2770)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     2980..2997
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     regulatory      8267..8276
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8670..8679
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    9231..9283
                     /label=MCS
                     /note="multiple cloning site"
     CDS             complement(9309..9998)
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
     LTR             10048..10316
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(10329..10540)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     repeat_region   10993..11055
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(11185..11202)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11185..11201)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11194..11216)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11343..11798
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11430..11449)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11640..11661
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11824..11928
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11929..11997,11998..12789)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12147..12166)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12960..13548
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13449..13468
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
       61 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
      121 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
      181 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
      241 acaggaaaca gctatgacca tgattacgcc aagcgcgccc gccgggtaac tcacggggta
      301 tccatgtcca tttctgcggc atccagccag gatacccgtc ctcgctgacg taatatccca
      361 gcgccgcacc gctgtcatta atctgcacac cggcacggca gttccggctg tcgccggtat
      421 tgttcgggtt gctgatgcgc ttcgggctga ccatccggaa ctgtgtccgg aaaagccgcg
      481 acgaactggt atcccaggtg gcctgaacga acagttcacc gttaaaggcg tgcatggcca
      541 caccttcccg aatcatcatg gtaaacgtgc gttttcgctc aacgtcaatg cagcagcagt
      601 catcctcggc aaactctttc catgccgctt caacctcgcg ggaaaaggca cgggcttctt
      661 cctccccgat gcccagatag cgccagcttg ggcgatgact gagccggaaa aaagacccga
      721 cgatatgatc ctgatgcagc tagattaacc ctagaaagat agtctgcgta aaattgacgc
      781 atgcattctt gaaatattgc tctctctttc taaatagcgc gaatccgtcg ctgtgcattt
      841 aggacatctc agtcgccgct tggagctccc gtgaggcgtg cttgtcaatg cggtaagtgt
      901 cactgatttt gaactataac gaccgcgtga gtcaaaatga cgcatgatta tcttttacgt
      961 gacttttaag atttaactca tacgataatt atattgttat ttcatgttct acttacgtga
     1021 taacttatta tatatatatt ttcttgttat agatatcagg gacagccccc ccccaaagcc
     1081 cccagggatg taattacgtc cctcccccgc tagggggcag cagcgagccg cccggggctc
     1141 cgctccggtc cggcgctccc cccgcatccc cgagccggca gcgtgcgggg acagcccggg
     1201 cacggggaag gtggcacggg atcgctttcc tctgaacgct tctcgctgct ctttgagcct
     1261 gcagacacct ggggggatac ggggaaaaag ccataccaat gggccctaaa aaaggaatcc
     1321 agtcaattcc ggggctaaac ctggctgcca ctgtttcttt agggacttcg ttcctgtgag
     1381 gacaggaaac cctgcaacct ggttgaccag agcaagggat ataagaaccc agtttgggaa
     1441 cactgctgtt taaatattaa acagggatgt cttgtccccc ggaagggaga ccagatgagc
     1501 ttctgataga ctaattgaca tcatttgagt caattccaga catgataaga tacattgatg
     1561 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     1621 atgctattgc tttatttgta accattataa gctgcaataa acaagtttag ttaacgcatg
     1681 atacaaaggc attaaagcag cgtatccaca tagcgtaaaa ggagcaacat agttaagaat
     1741 accagtcaat ctttcacaaa ttttgtaatc cagaggttga ttgtcgactc agaagaactc
     1801 gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac
     1861 gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc
     1921 tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg
     1981 gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcctc
     2041 gccgtcgggc atgcgcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg
     2101 ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc
     2161 gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg
     2221 ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag
     2281 atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc
     2341 gagcacagct gcgcaaggaa cgcccgtcgc ggccagccac gatagccgcg ctgcctcgtc
     2401 ctgcagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg
     2461 cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata
     2521 gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat
     2581 cattccggag gtattatcgt gtttttcaaa ggaaaaccac gtccccgtgg ttcggggggc
     2641 ctagacgttt ttttaacctc gactaaacac atgtaaagca tgtgcaccga ggccccagat
     2701 cagatcccat acaatggggt accttctggg catccttcag ccccttgttg aatacgcttg
     2761 aggagagcca tttgactctt tccacaacta tccaactcac aacgtggcac tggggttgtg
     2821 ccgcctttgc aggtgtatct tatacacgtg gcttttggcc gcagaggcac ctgtcgccag
     2881 gtggggggtt ccgctgcctg caaagggtcg ctacagacgt tgtttgtctt caagaagctt
     2941 ccagaggaac tgcttccttc acgacattca acagaccttg cattcctttg gcgagagggg
     3001 aaagacccct aggaatgctc gtcaagaaga cagggccagg tttccgggcc ctcacattgc
     3061 caaaagacgg caatatggtg gaaaataaca tatagacaaa cgcacaccgg ccttattcca
     3121 agcggcttcg gccagtaacg ttaggggggg gggagggaga ggggcgcggc cgcggatccg
     3181 atttaaattc gaacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
     3241 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatcagt
     3301 tatctagcgt tctcctcgtc actgtcatcc gggctgatag ccgggcttgt gagactgtag
     3361 gtggggctgg tgggcgagta accaggggaa gtgggagagt aggttgagcc tttgggggag
     3421 gtgggcgagt aggtggggct ggtgggcgag tacttggggg aagtgggcga gtaagtgggg
     3481 ctagtaggtg agtacttggg agaggttggg gtgtagactg gagaggttgg ggaataagta
     3541 ggagatgttg gggagtattt tggggtggtg ggtgaatagg tgggactggt aggcgagtac
     3601 ttgggagagg tgggtgaata tttgggactg gtaggtgagt acttgggaga ggttggggta
     3661 tactctgggg agctgggact gtaagaagga ctggttgggg tgtacttggg tgaggttggg
     3721 ctgtagctgg gcgagctggg gctgtagctg ggtgagcttg gggtataggt tggagactgt
     3781 ggtgtgtatc gtgggctgga aggggagtaa cttggtgagg tcggggaata gctgggtgat
     3841 gttggagagt agcttggaga ggttgggctg tagttagggc tggtaggtgt gtagttggga
     3901 ctagtaggtg aatagctggg tgatgtcggg ctgtagctgg gtgatgttgg ggtgtaattg
     3961 ggactggttg gagaatagtt cgggctggtg ggtgagtaac ttggggaagt gggtgagtag
     4021 ctgggggagg tgggtgaata gcttggagag gtaggtgagt agctgggaga agtgggagaa
     4081 tagctgggtg aagttggcga gtagctggga gatgtcggcg agtagctggg agacgttggc
     4141 gagtagctag gggaagtggg cgagtagcta ggggaagtgg gtgagtagct gggagaggtg
     4201 ggcgagtagc tgggagaggt gggcgagtag ctgggagagg tgggtgagta gctgggagag
     4261 gtcggtgagt agctgggtga cgttggcgaa tagctgggtg atgtgggact atagttggga
     4321 ctggttggag aataggatgg agaggtaggg gagtaggagg gtgaagtggg ggaataagag
     4381 ggactctggg gtgtgtagcc cccaggagag cggggctcgt aggcaggtga cgttggcgag
     4441 tagctgggag acatggcacc acctggtgaa gggatgtagg ggcttgaggg acctggggac
     4501 cccggggagc ccggtgtggg agaccaggca ggggagtaac ctgggctgaa gccgctggca
     4561 tctgacgcag cactgggaga gaagccggct gcccctgggg tcattccact cccaacactg
     4621 ggggaccagg cgccataggc aggggttgca ccctggttcc aaggtgtcat ggcaggagag
     4681 attccaccca tgggactggg tgctgaacca aagaacatgc cggtgggtcc agcagccccc
     4741 aggccgggga tattggtggg gatctccatg ccatacttgc acttctctgc atcaagcagg
     4801 aggtcaaagc agccagtgcc ggccggagcc agctggccca gcatgatatt ctcagagacc
     4861 cccttcatgg ggtcactctc accgtgtgcg gctgcttcca taagcacgtc caccgtttcc
     4921 tcaaaggaac acttcatgag tggtcctgtg tcctggcggt tgactccgtg tcgggtgatg
     4981 gccatcaagt ggccacgaca ggtcatggta tcacacaaga gagccaagtg tcggtaattg
     5041 acataggagc catcaaagga gatgacgtgg tacagctccc gctccagggc cttccgcacg
     5101 gcttcaatgc ccagcaccgt gaagatctcc acaatgtcat tggacgtggt gcgtacgggg
     5161 tccacgtcct tctcactcag cacccgcatc aagctcacgc cgtccgtctc caggatccac
     5221 tcctgcaggg ccttgaattc cccatcctcc gtgatgatga tcttcttctt gttgtctgtc
     5281 tgtggcaagt gcatgtacac cttgctgatc tgctcgatgc cctgcagggt catatctgtc
     5341 agcatgttgg actcgatgca gcgcaggaag acatcatcat ccatcttgtc caccacctct
     5401 tcctcctctt gcatcttgtt ctcatcgctg ttcatgatgc gaatacggag caccagcttc
     5461 tctgcattgt catcattaaa gatgcagttc aagtcgtcac caaaaccagc attgatcttt
     5521 tcagcaatct gctccatggt gagcttccgg tcagtcatgt gcttccgatc cagctccacc
     5581 cgcaacagcc agggggagat tcgggccaca tcaaagtcag gcatttcata gtagacattc
     5641 acccattcct gatcctctgc caccaccgtg ctctgggggt tggggtcata gtagatggct
     5701 gtgttggcag tcaccttcct caacgttgta tgctccagac ggcacagaat atccttggct
     5761 ctctcagcat ctcgagcgga ctggcccaac aggaagacag taagcgaagg agtctttggc
     5821 ttcttggaaa tgttgatgag ctccttaagt cggggcacac ccagcgtcac attcttggca
     5881 gacacaccag catagtggaa ggtattcaag gtcatctggg tggcaggttc tccaagggac
     5941 tgcgcagcca gagcccccac catttccccg ggatgcgcaa tggcttggtt gaacttggac
     6001 tcaatctccc caagcagcca gtcgaaggcc tccccactga gccgaaactc ctctgccatg
     6061 cggcgggaac acaacgtgga ccgcaggtgg atgttgaaga gcagcgtggc attttcctgg
     6121 gcctgtcgac ttagtgggtc atccccattc acaatcacca gcttcttgct caattccttg
     6181 actccctcca ccactttgat ggggtgcagg tcggagggaa ggcgtgggtt gatgtggaag
     6241 attttctgag cattccagat catccgcagc aggttacagg ggaggacgac cttgctgtct
     6301 ccagttggga agatgaccct gagcacctcc cgatcctccc gcatccgctc aaattcccgc
     6361 tccaactcgt tctggatgtg tgcgttgctc agcacgtcct tcaccaggtc ctcctgcaga
     6421 gtgcgccgca gggccctctc attggtataa tcaaagcgga acttcttctc aaaagccttg
     6481 ttggaaggct taagcgtagc caggttctgg aactcaacgc tctcgcctgc caggccgtct
     6541 tcgccgtagc gcagctgcac cacctggttg atggagttcc gcacagtcgc gtcgtacttc
     6601 accatcactg actccatgga cttgatcagc cgccgctgga tgtatccagt ctcagcagtc
     6661 ttgacagccg tgtcaatgag cccctcacga ccccccatgg cgtggaaaaa gaactcagtg
     6721 ggtgtgaggc cggctaggta ggagttctcc acaaagccac ggctctcagg cccgtagtca
     6781 tccttgatga agtgaggcag agtccggtgc ttgaagccaa atggaatccg cttgccctcg
     6841 acgtcctgct gtccaacgac agcaatgacc tgggagatgt taatcttgga acctttagct
     6901 ccggacacga ccatagactt gaagttgttg tattcagaca gggatttctg agcagaggag
     6961 ccagtcttgt ctcgggcatc gttaagaatg cggttcacct gattctcaaa cgtctgccgc
     7021 agagtgttcc ctggggtggg ctccagctca ttgttgtgtg ccttctcgat gacctctatt
     7081 acgtcctgct tggccttctt aatagtgttc tgaatgtcct ggtaagtctt agaatcagca
     7141 atggagtccc caatgccaat agtatgaccc tcgatgagga gccagttgtt aatgacagtc
     7201 tgaatgttgg agtagaagag gcgagtgatg tcatgaccca tctctaggta ggagatgtgg
     7261 accagggagc cagctgacgt gcccagagac ttcttacaca ggatgcccat gatcagctcc
     7321 ccattctcca ccaccacctt ggtgtcccca ggagagatgt gcttgtaagg gccactgtct
     7381 tcatcatcgg gatgggtgct gtgggtacgg atacaattga tgtgaccagg tatgatgagg
     7441 gagaagattt gcttgcctgt ccacaggggc cggggcttta ggatggccgg ctgtgggacc
     7501 ttcccatccc acgtcgacag gaacatcagg aggttcatca cttcaccccg ctccaggaag
     7561 acgtctctct tggtgaattt gcgcactgct gtgagtgtgt cctgcacaat acccatgaca
     7621 ggccgattgc tctggggggt gacaatcatg cgaggaacca tggccagctc ctggatctct
     7681 gctcgcgtct ccagagactg tggcaggtgc aagttcatct catccccgtc aaagtctgca
     7741 ttgtacggag ttgtcacgct aagattcaag cgaaaggtag accatgggag aatgcggacc
     7801 cgatgcccca tcatggacat tttgtgcaga gttggctgcc ggttgaagat aacaatgtcc
     7861 ccatcacaca tgtgccgttc caccttatag ccggtctgca ggtgaaggtc actgggcttg
     7921 gggtggaaac gcaagtcaat gcgatcacca ttgtctcgga tgatgtactt ggcgcctggg
     7981 tactggctgt tccccctgcg cactagttct tgaagtctgt caatgttgaa gggggtgaca
     8041 atctccgcaa aggtcatgtt ggcagcaatg gagcggggca cgccaacctg gtcaatggag
     8101 aggttggggt cgggggtgat gacagtacgg gccgagaagt ccactctttt gcccatcagg
     8161 ttccctcgca cccggccttc cttgcccttc aaccgctgct tcagggactt gaggggacgc
     8221 ccagacttct gcatggcacg gggcaagcca ggcagctcat tgtccaccat ggtggccaca
     8281 tggaactgga ggagcttcac atcctctgca atgacatggg ccgctgcgcc gttctgctca
     8341 ttgcgccgca gctgattgtt gatcttcacg atgtcagcca gtttgtgagt caggtcatcc
     8401 tggttacggg cagagccctg catcacaaca gcaggccgca cggagagcgg gggcacaggc
     8461 agcactgtga caatcatcca ctctggccgt gcatagcggg gctccatgcc cagcacaaaa
     8521 cactcctcat ctgagatgcg tttgaagatc tcatgcactc gctctggact cagcaggatc
     8581 ttcttctcct gagagtcctc attaacgtgc ttccattccg catacagctc taggccagaa
     8641 cgccggatcc tgggctggta ccgcccacag ccaccatggc ccttttcttt ggtcagatcc
     8701 tcgtcaccct caggttgttc cacaccgaac ttgttgtcca tctcctcccc accctcgcat
     8761 atgtttttgc ccttgcaaag gtcgtagaca tgtgtgagcc gcttcttggg ctgtcccttg
     8821 gacttagcca ggatatcctt gatctttggg ttgttagagt ccacaagcag tttggagcag
     8881 aagaagcaga cacagcgcaa aactttcatt gtcttcacca ggaagcccac gtgaaacaca
     8941 ggcttggcca gttcaatgtg gccaaagtgg ccaggacact ctgtcatgtt tcctgcacat
     9001 gtttggcagc ggccagtccg ctcaatcacc ccctgcctcg ggtccatcag ccccccaagc
     9061 ttggggcggc ctccctcagt cgtctctggg tatttgatgc caccctccgt cacagacatt
     9121 cgcttcagtt catccggact caggactccg aactggactc tcttgatggt gcgcagcggg
     9181 catgcgctgt cccccgaggg ggggccaccc ccgtgcatgc tagatccggt ggatcccggg
     9241 cccgcggtac cgtcgactgc agaattcgaa gcttgagctc gagtcttgta cacgccgctg
     9301 tcgccggacc acacctggct gggcaggggg ctgtagcggg ccacggcgtg ctcgtacagc
     9361 ttcaccttgt tgtagtcgct gtcgttgccc aggatctcga tgcggtggtc cacgaagtgg
     9421 gcgtcgggca gctgcaccac cttcttggcc ttgtaggtgg tcttgaagtc gcacaggtag
     9481 tggccgccgc cctccagcag cagggccatg ttgatgttgc ccaccagcag gccgtcgcgc
     9541 acgtgcagct tctcggtgct gggctcccac ttcagggtct tcttctgcat cacggggccg
     9601 ttggggggga agttggtgcc cttgaagcgc acgttctgga agaagcagtc gccctccagg
     9661 ctgatgtcgc tgcggatggt gcagatgccc ttgtcctcga aggtcatggt gcgctcccag
     9721 ctgtagccct cggggaagct ctgcttgaag tagtcgggga tgtcctcggg gtacttggtg
     9781 aacacccggt tgccgtagtg cacggcggtg gtcaggatgt cgtagctgaa gggcaggggg
     9841 gcgccctcct tcacggtcag gttggcggtc tgggtgccct cgtagggctt gcccttgccc
     9901 tcgccctcga tcacgaaggc gtggccgttc acgttgccct ccatgtgcac cttcacgcgc
     9961 atgtcctcct tgatcaggtt aattcccggg gtgttcatgg tggcgaccgg tagcgctagc
    10021 tctagagtag gcgccggtca cagcttggat ctgtaacggc gcagaacaga aaacgaaaca
    10081 aagacgtaga gttgagcaag cagggtcagg caaagcgtgg agagccggct gagtctaggt
    10141 aggctccaag ggagcgccgg acaaaggccc ggtctcgacc tgagctttaa acttacctag
    10201 acggcggacg cagttcagga ggcaccacag gcgggaggcg gcagaacgcg actcaaccgg
    10261 cgtggatggc ggcctcaggt agggcggcgg gcgcgtgaag gagagatgcg agcccctcga
    10321 agcttcagct gtgttctggc ggcaaacccg ttgcgaaaaa gaacgttcac ggcgactact
    10381 gcacttatat acggttctcc cccaccctcg ggaaaaaggc ggagccagta cacgacatca
    10441 ctttcccagt ttaccccgcg ccaccttctc taggcacccg ttcaattgcc gacccctccc
    10501 cccaacttct cggggactgt gggcgatgtg cgctctgccc actgacgggc accggagcga
    10561 tcgcagatcc ttggccgtgg aggccttttc cccgtatccc cccaggtgtc tgcaggctca
    10621 aagagcagcg agaagcgttc agaggaaagc gatcccgtgc caccttcccc gtgcccgggc
    10681 tgtccccgca cgctgccggc tcggggatgc ggggggagcg ccggaccgga gcggagcccc
    10741 gggcggctcg ctgctgcccc ctagcggggg agggacgtaa ttacatccct gggggctttg
    10801 ggggggggct gtccctcata aaagttttgt tactttatag aagaaatttt gagtttttgt
    10861 tttttttaat aaataaataa acataaataa attgtttgtt gaatttatta ttagtatgta
    10921 agtgtaaata taataaaact taatatctat tcaaattaat aaataaacct cgatatacag
    10981 accgataaaa cacatgcgtc aattttacac atgattatct ttaacgtacg tcacaatatg
    11041 attatctttc tagggttaat ctagctgcgt gttctgcagc gtgtcgagca tcttcatctg
    11101 ctccatcacg ctgtaaaaca catttgcacc gcgagtctgc ccgtcctcca cgggttcaaa
    11161 aacgtgaatg aacgaggcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
    11221 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
    11281 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
    11341 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
    11401 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
    11461 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
    11521 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
    11581 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
    11641 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
    11701 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
    11761 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
    11821 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
    11881 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
    11941 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
    12001 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
    12061 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
    12121 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
    12181 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
    12241 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
    12301 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
    12361 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
    12421 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
    12481 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
    12541 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
    12601 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
    12661 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
    12721 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
    12781 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
    12841 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
    12901 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
    12961 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
    13021 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
    13081 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
    13141 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
    13201 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
    13261 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
    13321 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
    13381 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
    13441 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
    13501 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
    13561 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
    13621 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
    13681 cgcagccgaa cg
//
