LOCUS       sequence-394115-       14378 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Derived from pLV hU6-sgRNA Nestin-dCas9-KRAB-T2a-GFP with non-target
            sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14378)
  AUTHORS   Dause TJ, Osap R, Kuwahara AA, Denninger JK, Kirby ED
  TITLE     Intracrine VEGF Signaling Is Required for Adult Hippocampal Neural 
            Stem Cell Maintenance and Vascular Proximity.
  JOURNAL   Mol Neurobiol. 2025 Mar 25. doi: 10.1007/s12035-025-04861-1.
  PUBMED    40131696
REFERENCE   2  (bases 1 to 14378)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Neurobiol. 2025 Mar 25. doi: 10.1007/s12035-025-04861-1."
COMMENT     Sequence Label: pLV hU6-Non-target sgRNA Nestin-dCas9-KRAB-T2a...
FEATURES             Location/Qualifiers
     source          1..14378
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(88..107)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        279..658
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        660..858
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     813..833
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             876..1056
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1103..1228
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1721..1954
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2139..2183
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2481..2598
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2642..2882
                     /label=U6 promoter
                     /note="Truncated RNA polymerase III promoter for human U6 
                     snRNA used in pSIREN vectors such as 
                     pSIREN-RetroQ-ZsGreen1. Expected transcription start site 
                     is 8bp downstream of this feature."
     primer_bind     2642..2662
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2813..2832
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2904..2979
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     regulatory      3695..3704
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3704..3769
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3776..3796
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3806..3832,3833..3835,3836..6319,6320..6325,6326..6391,
                     6392..6394,6395..7909)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(3806..3832,3833..3835,3836..6322,6323..6325,
                     6326..7909)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3806..7909
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7922..7942
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7976..8170
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQILYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     CDS             8237..8257
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8264..8317
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(8324..8326,8327..9037)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(8324..8326,8327..9037)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(8366..8387)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8627..8646)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8974..8995
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(9053..9086)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    9111..9699
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9164..9184)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9582..9593)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9702..9718)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9703..9719)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10224..10404
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10430..10447)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10436..10660
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10706..11134
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10793..10812)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11003..11024
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11143..11163)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11148..11477
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11328..11463
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11390..11409
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        11525..11572
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             11591..11965
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    12095..12216
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12132..12151)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12186..12205
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12265..12281)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12265..12281)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12278..12300)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12289..12305
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12313..12319,12320..12337,12338..12343))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12358..12379
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12496..12513)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12667..13255)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12747..12766)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13426..14217,14218..14286))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14049..14068
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(14287..14378,1..13))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
       61 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
      121 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
      181 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
      241 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta
      301 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      361 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      421 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      481 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      541 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      601 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      661 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
      721 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      781 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
      841 ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct
      901 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
      961 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     1021 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     1081 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     1141 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     1201 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     1261 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     1321 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     1381 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     1441 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     1501 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     1561 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     1621 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     1681 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     1741 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     1801 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     1861 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     1921 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     1981 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     2041 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     2101 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     2161 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     2221 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     2281 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     2341 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     2401 gagacagatc cattcgatta gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa
     2461 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     2521 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     2581 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttggttaatt
     2641 agagggccta tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga
     2701 gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag
     2761 aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca
     2821 tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg
     2881 acggcgaggt attcggctcc gcggttttag agctagaaat agcaagttaa aataaggcta
     2941 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttttgtttt ttaattaatt
     3001 aacccgtgtc ggctccagat ctgatccctg aagagtttgt gatcctgaga tgagggcttt
     3061 agccccagtc agtcctctga ggggaagggt ccaggcagct ctgaggaatg taaccactgg
     3121 cgtttgaggt ctgaaaagga tttggagaag gggagctgaa ttcatttgct tttgtctgtt
     3181 accagctctg ggggcagaga gagagccatc ccctgggaac agcctgagaa ttcccacttc
     3241 ccctgaggag ccctcccttc ttaggccctc cagatggtag tgtggacaaa aggcaataat
     3301 tagcatgaga atcggcctcc ctcccagagg atgaggtcat cggccttggc cttgggtggg
     3361 gaggcggaga ctgatctgag gagtctgata taagtgttag caattcattt ggccctgcct
     3421 ccgactgtgg gaatctgcat gtggggtctc cctgtgtctc aaatatgggt tggctaagta
     3481 tatatctgtg ggtatatgac tgtgtggctt ttatatgaca atggtcacaa tagagattga
     3541 tcctgcagtg gcaggacatg ctacctcagc tggagctgac cctatctccc cactccccac
     3601 caggactctg ctggaggctg agaactctcg gttgcagaca cctggacgag gttcccaggc
     3661 ttaatcgaag cttgggctgc aggtcgactc tagagccacc atggactaca aagaccatga
     3721 cggtgattat aaagatcatg acatcgatta caaggatgac gatgacaaga tggcccccaa
     3781 gaagaagagg aaggtgggcc gcggaatgga caagaagtac tccattgggc tcgccatcgg
     3841 cacaaacagc gtcggctggg ccgtcattac ggacgagtac aaggtgccga gcaaaaaatt
     3901 caaagttctg ggcaataccg atcgccacag cataaagaag aacctcattg gcgccctcct
     3961 gttcgactcc ggggaaaccg ccgaagccac gcggctcaaa agaacagcac ggcgcagata
     4021 tacccgcaga aagaatcgga tctgctacct gcaggagatc tttagtaatg agatggctaa
     4081 ggtggatgac tctttcttcc ataggctgga ggagtccttt ttggtggagg aggataaaaa
     4141 gcacgagcgc cacccaatct ttggcaatat cgtggacgag gtggcgtacc atgaaaagta
     4201 cccaaccata tatcatctga ggaagaagct tgtagacagt actgataagg ctgacttgcg
     4261 gttgatctat ctcgcgctgg cgcatatgat caaatttcgg ggacacttcc tcatcgaggg
     4321 ggacctgaac ccagacaaca gcgatgtcga caaactcttt atccaactgg ttcagactta
     4381 caatcagctt ttcgaagaga acccgatcaa cgcatccgga gttgacgcca aagcaatcct
     4441 gagcgctagg ctgtccaaat cccggcggct cgaaaacctc atcgcacagc tccctgggga
     4501 gaagaagaac ggcctgtttg gtaatcttat cgccctgtca ctcgggctga cccccaactt
     4561 taaatctaac ttcgacctgg ccgaagatgc caagcttcaa ctgagcaaag acacctacga
     4621 tgatgatctc gacaatctgc tggcccagat cggcgaccag tacgcagacc tttttttggc
     4681 ggcaaagaac ctgtcagacg ccattctgct gagtgatatt ctgcgagtga acacggagat
     4741 caccaaagct ccgctgagcg ctagtatgat caagcgctat gatgagcacc accaagactt
     4801 gactttgctg aaggcccttg tcagacagca actgcctgag aagtacaagg aaattttctt
     4861 cgatcagtct aaaaatggct acgccggata cattgacggc ggagcaagcc aggaggaatt
     4921 ttacaaattt attaagccca tcttggaaaa aatggacggc accgaggagc tgctggtaaa
     4981 gcttaacaga gaagatctgt tgcgcaaaca gcgcactttc gacaatggaa gcatccccca
     5041 ccagattcac ctgggcgaac tgcacgctat cctcaggcgg caagaggatt tctacccctt
     5101 tttgaaagat aacagggaaa agattgagaa aatcctcaca tttcggatac cctactatgt
     5161 aggccccctc gcccggggaa attccagatt cgcgtggatg actcgcaaat cagaagagac
     5221 catcactccc tggaacttcg aggaagtcgt ggataagggg gcctctgccc agtccttcat
     5281 cgaaaggatg actaactttg ataaaaatct gcctaacgaa aaggtgcttc ctaaacactc
     5341 tctgctgtac gagtacttca cagtttataa cgagctcacc aaggtcaaat acgtcacaga
     5401 agggatgaga aagccagcat tcctgtctgg agagcagaag aaagctatcg tggacctcct
     5461 cttcaagacg aaccggaaag ttaccgtgaa acagctcaaa gaagactatt tcaaaaagat
     5521 tgaatgtttc gactctgttg aaatcagcgg agtggaggat cgcttcaacg catccctggg
     5581 aacgtatcac gatctcctga aaatcattaa agacaaggac ttcctggaca atgaggagaa
     5641 cgaggacatt cttgaggaca ttgtcctcac ccttacgttg tttgaagata gggagatgat
     5701 tgaagaacgc ttgaaaactt acgctcatct cttcgacgac aaagtcatga aacagctcaa
     5761 gaggcgccga tatacaggat gggggcggct gtcaagaaaa ctgatcaatg ggatccgaga
     5821 caagcagagt ggaaagacaa tcctggattt tcttaagtcc gatggatttg ccaaccggaa
     5881 cttcatgcag ttgatccatg atgactctct cacctttaag gaggacatcc agaaagcaca
     5941 agtttctggc cagggggaca gtcttcacga gcacatcgct aatcttgcag gtagcccagc
     6001 tatcaaaaag ggaatactgc agaccgttaa ggtcgtggat gaactcgtca aagtaatggg
     6061 aaggcataag cccgagaata tcgttatcga gatggcccga gagaaccaaa ctacccagaa
     6121 gggacagaag aacagtaggg aaaggatgaa gaggattgaa gagggtataa aagaactggg
     6181 gtcccaaatc cttaaggaac acccagttga aaacacccag cttcagaatg agaagctcta
     6241 cctgtactac ctgcagaacg gcagggacat gtacgtggat caggaactgg acatcaatcg
     6301 gctctccgac tacgacgtgg atgccatcgt gccccagtct tttctcaaag atgattctat
     6361 tgataataaa gtgttgacaa gatccgataa aaatagaggg aagagtgata acgtcccctc
     6421 agaagaagtt gtcaagaaaa tgaaaaatta ttggcggcag ctgctgaacg ccaaactgat
     6481 cacacaacgg aagttcgata atctgactaa ggctgaacga ggtggcctgt ctgagttgga
     6541 taaagccggc ttcatcaaaa ggcagcttgt tgagacacgc cagatcacca agcacgtggc
     6601 ccaaattctc gattcacgca tgaacaccaa gtacgatgaa aatgacaaac tgattcgaga
     6661 ggtgaaagtt attactctga agtctaagct ggtctcagat ttcagaaagg actttcagtt
     6721 ttataaggtg agagagatca acaattacca ccatgcgcat gatgcctacc tgaatgcagt
     6781 ggtaggcact gcacttatca aaaaatatcc caagcttgaa tctgaatttg tttacggaga
     6841 ctataaagtg tacgatgtta ggaaaatgat cgcaaagtct gagcaggaaa taggcaaggc
     6901 caccgctaag tacttctttt acagcaatat tatgaatttt ttcaagaccg agattacact
     6961 ggccaatgga gagattcgga agcgaccact tatcgaaaca aacggagaaa caggagaaat
     7021 cgtgtgggac aagggtaggg atttcgcgac agtccggaag gtcctgtcca tgccgcaggt
     7081 gaacatcgtt aaaaagaccg aagtacagac cggaggcttc tccaaggaaa gtatcctccc
     7141 gaaaaggaac agcgacaagc tgatcgcacg caaaaaagat tgggacccca agaaatacgg
     7201 cggattcgat tctcctacag tcgcttacag tgtactggtt gtggccaaag tggagaaagg
     7261 gaagtctaaa aaactcaaaa gcgtcaagga actgctgggc atcacaatca tggagcgatc
     7321 aagcttcgaa aaaaacccca tcgactttct cgaggcgaaa ggatataaag aggtcaaaaa
     7381 agacctcatc attaagcttc ccaagtactc tctctttgag cttgaaaacg gccggaaacg
     7441 aatgctcgct agtgcgggcg agctgcagaa aggtaacgag ctggcactgc cctctaaata
     7501 cgttaatttc ttgtatctgg ccagccacta tgaaaagctc aaagggtctc ccgaagataa
     7561 tgagcagaag cagctgttcg tggaacaaca caaacactac cttgatgaga tcatcgagca
     7621 aataagcgaa ttctccaaaa gagtgatcct cgccgacgct aacctcgata aggtgctttc
     7681 tgcttacaat aagcacaggg ataagcccat cagggagcag gcagaaaaca ttatccactt
     7741 gtttactctg accaacttgg gcgcgcctgc agccttcaag tacttcgaca ccaccataga
     7801 cagaaagcgg tacacctcta caaaggaggt cctggacgcc acactgattc atcagtcaat
     7861 tacggggctc tatgaaacaa gaatcgacct ctctcagctc ggtggagaca gcagggctga
     7921 ccccaagaag aagaggaagg tggctagcga tgctaagtca ctgactgcct ggtcccggac
     7981 actggtgacc ttcaaggatg tgtttgtgga cttcaccagg gaggagtgga agctgctgga
     8041 cactgctcag cagatcctgt acagaaatgt gatgctggag aactataaga acctggtttc
     8101 cttgggttat cagcttacta agccagatgt gatcctccgg ttggagaagg gagaagagcc
     8161 ctggctggtg gagagagaaa ttcaccaaga gacccatcct gattcagaga ctgcatttga
     8221 aatcaaatca tcagttccga aaaagaaacg caaagttgct agcgagggca gaggaagtct
     8281 tctaacatgc ggtgacgtgg aggagaatcc cggccctggt accgtgagca agggcgagga
     8341 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     8401 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     8461 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     8521 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     8581 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     8641 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     8701 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     8761 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     8821 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     8881 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     8941 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     9001 cgccgggatc actctcggca tggacgagct gtacaagacc ggttgataat agataacttc
     9061 gtatagcata cattatacga agttatgaat tcgatatcaa gcttatcgat aatcaacctc
     9121 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     9181 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     9241 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     9301 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     9361 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     9421 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     9481 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     9541 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     9601 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     9661 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag
     9721 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
     9781 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
     9841 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     9901 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
     9961 acacacaagg ctacttccct gattggcaga actacacacc agggccaggg atcagatatc
    10021 cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag
    10081 ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc
    10141 cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc
    10201 gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc
    10261 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
    10321 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
    10381 agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg
    10441 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
    10501 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
    10561 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
    10621 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
    10681 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
    10741 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
    10801 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
    10861 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
    10921 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
    10981 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
    11041 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
    11101 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
    11161 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
    11221 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
    11281 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
    11341 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
    11401 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
    11461 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc
    11521 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
    11581 gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc
    11641 cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga
    11701 cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt
    11761 ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga
    11821 gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat
    11881 cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca
    11941 cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt
    12001 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
    12061 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
    12121 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
    12181 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
    12241 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
    12301 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
    12361 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
    12421 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
    12481 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
    12541 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
    12601 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
    12661 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
    12721 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
    12781 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    12841 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    12901 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    12961 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    13021 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
    13081 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
    13141 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
    13201 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    13261 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    13321 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
    13381 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
    13441 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
    13501 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
    13561 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
    13621 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
    13681 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
    13741 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
    13801 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    13861 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    13921 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    13981 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    14041 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    14101 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    14161 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    14221 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    14281 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    14341 cggatacata tttgaatgta tttagaaaaa taaacaaa
//
