LOCUS       sequence-394116-       14371 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Derived from pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP, custom sgRNA 
            cloning vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14371)
  AUTHORS   Dause TJ, Osap R, Kuwahara AA, Denninger JK, Kirby ED
  TITLE     Intracrine VEGF Signaling Is Required for Adult Hippocampal Neural 
            Stem Cell Maintenance and Vascular Proximity.
  JOURNAL   Mol Neurobiol. 2025 Mar 25. doi: 10.1007/s12035-025-04861-1.
  PUBMED    40131696
REFERENCE   2  (bases 1 to 14371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Neurobiol. 2025 Mar 25. doi: 10.1007/s12035-025-04861-1."
COMMENT     Sequence Label: pLV hU6-sgRNA Nestin-dCas9-KRAB-T2a-GFP
FEATURES             Location/Qualifiers
     source          1..14371
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(63..82)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        254..633
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        635..833
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     788..808
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             851..1031
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1078..1203
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1696..1929
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2114..2158
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2456..2573
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2617..2857
                     /label=U6 promoter
                     /note="Truncated RNA polymerase III promoter for human U6 
                     snRNA used in pSIREN vectors such as 
                     pSIREN-RetroQ-ZsGreen1. Expected transcription start site 
                     is 8bp downstream of this feature."
     primer_bind     2617..2637
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2788..2807
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2872..2947
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     regulatory      3663..3672
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3672..3737
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3744..3764
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3774..3800,3801..3803,3804..6287,6288..6293,6294..6359,
                     6360..6362,6363..7877)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(3774..3800,3801..3803,3804..6290,6291..6293,
                     6294..7877)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3774..7877
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7890..7910
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7944..8138
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQILYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     CDS             8205..8225
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8232..8285
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(8292..8294,8295..9005)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(8292..8294,8295..9005)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(8334..8355)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8595..8614)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8942..8963
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(9021..9054)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    9079..9667
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9132..9152)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9550..9561)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9670..9686)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9671..9687)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10192..10372
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10398..10415)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10404..10628
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10674..11102
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10761..10780)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10971..10992
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11111..11131)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11116..11445
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11296..11431
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11358..11377
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        11493..11540
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             11559..11933
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    12063..12184
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12100..12119)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12154..12173
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12233..12249)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12233..12249)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12246..12268)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12257..12273
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12281..12287,12288..12305,12306..12311))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12326..12347
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12464..12481)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12635..13223)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12715..12734)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13394..14185,14186..14254))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14017..14036
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14255..14359)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc
       61 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg
      121 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac
      181 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
      241 agatatacgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
      301 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      361 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      421 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      481 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      541 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      601 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      661 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      721 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      781 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagcgcgttt
      841 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
      901 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
      961 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     1021 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     1081 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     1141 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     1201 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     1261 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     1321 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     1381 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     1441 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     1501 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     1561 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     1621 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     1681 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     1741 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     1801 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     1861 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     1921 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     1981 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     2041 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     2101 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     2161 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     2221 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     2281 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
     2341 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
     2401 cggatcggca ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta
     2461 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     2521 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     2581 attacaggga cagcagagat ccagtttggt taattagagg gcctatttcc catgattcct
     2641 tcatatttgc atatacgata caaggctgtt agagagataa ttggaattaa tttgactgta
     2701 aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt
     2761 gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat
     2821 ttcgatttct tggctttata tatcttgtgg aaaggactga gacgcgtctc agttttagag
     2881 ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag
     2941 tcggtgcttt tttgtttttt aattaattaa cccgtgtcgg ctccagatct gatccctgaa
     3001 gagtttgtga tcctgagatg agggctttag ccccagtcag tcctctgagg ggaagggtcc
     3061 aggcagctct gaggaatgta accactggcg tttgaggtct gaaaaggatt tggagaaggg
     3121 gagctgaatt catttgcttt tgtctgttac cagctctggg ggcagagaga gagccatccc
     3181 ctgggaacag cctgagaatt cccacttccc ctgaggagcc ctcccttctt aggccctcca
     3241 gatggtagtg tggacaaaag gcaataatta gcatgagaat cggcctccct cccagaggat
     3301 gaggtcatcg gccttggcct tgggtgggga ggcggagact gatctgagga gtctgatata
     3361 agtgttagca attcatttgg ccctgcctcc gactgtggga atctgcatgt ggggtctccc
     3421 tgtgtctcaa atatgggttg gctaagtata tatctgtggg tatatgactg tgtggctttt
     3481 atatgacaat ggtcacaata gagattgatc ctgcagtggc aggacatgct acctcagctg
     3541 gagctgaccc tatctcccca ctccccacca ggactctgct ggaggctgag aactctcggt
     3601 tgcagacacc tggacgaggt tcccaggctt aatcgaagct tgggctgcag gtcgactcta
     3661 gagccaccat ggactacaaa gaccatgacg gtgattataa agatcatgac atcgattaca
     3721 aggatgacga tgacaagatg gcccccaaga agaagaggaa ggtgggccgc ggaatggaca
     3781 agaagtactc cattgggctc gccatcggca caaacagcgt cggctgggcc gtcattacgg
     3841 acgagtacaa ggtgccgagc aaaaaattca aagttctggg caataccgat cgccacagca
     3901 taaagaagaa cctcattggc gccctcctgt tcgactccgg ggaaaccgcc gaagccacgc
     3961 ggctcaaaag aacagcacgg cgcagatata cccgcagaaa gaatcggatc tgctacctgc
     4021 aggagatctt tagtaatgag atggctaagg tggatgactc tttcttccat aggctggagg
     4081 agtccttttt ggtggaggag gataaaaagc acgagcgcca cccaatcttt ggcaatatcg
     4141 tggacgaggt ggcgtaccat gaaaagtacc caaccatata tcatctgagg aagaagcttg
     4201 tagacagtac tgataaggct gacttgcggt tgatctatct cgcgctggcg catatgatca
     4261 aatttcgggg acacttcctc atcgaggggg acctgaaccc agacaacagc gatgtcgaca
     4321 aactctttat ccaactggtt cagacttaca atcagctttt cgaagagaac ccgatcaacg
     4381 catccggagt tgacgccaaa gcaatcctga gcgctaggct gtccaaatcc cggcggctcg
     4441 aaaacctcat cgcacagctc cctggggaga agaagaacgg cctgtttggt aatcttatcg
     4501 ccctgtcact cgggctgacc cccaacttta aatctaactt cgacctggcc gaagatgcca
     4561 agcttcaact gagcaaagac acctacgatg atgatctcga caatctgctg gcccagatcg
     4621 gcgaccagta cgcagacctt tttttggcgg caaagaacct gtcagacgcc attctgctga
     4681 gtgatattct gcgagtgaac acggagatca ccaaagctcc gctgagcgct agtatgatca
     4741 agcgctatga tgagcaccac caagacttga ctttgctgaa ggcccttgtc agacagcaac
     4801 tgcctgagaa gtacaaggaa attttcttcg atcagtctaa aaatggctac gccggataca
     4861 ttgacggcgg agcaagccag gaggaatttt acaaatttat taagcccatc ttggaaaaaa
     4921 tggacggcac cgaggagctg ctggtaaagc ttaacagaga agatctgttg cgcaaacagc
     4981 gcactttcga caatggaagc atcccccacc agattcacct gggcgaactg cacgctatcc
     5041 tcaggcggca agaggatttc tacccctttt tgaaagataa cagggaaaag attgagaaaa
     5101 tcctcacatt tcggataccc tactatgtag gccccctcgc ccggggaaat tccagattcg
     5161 cgtggatgac tcgcaaatca gaagagacca tcactccctg gaacttcgag gaagtcgtgg
     5221 ataagggggc ctctgcccag tccttcatcg aaaggatgac taactttgat aaaaatctgc
     5281 ctaacgaaaa ggtgcttcct aaacactctc tgctgtacga gtacttcaca gtttataacg
     5341 agctcaccaa ggtcaaatac gtcacagaag ggatgagaaa gccagcattc ctgtctggag
     5401 agcagaagaa agctatcgtg gacctcctct tcaagacgaa ccggaaagtt accgtgaaac
     5461 agctcaaaga agactatttc aaaaagattg aatgtttcga ctctgttgaa atcagcggag
     5521 tggaggatcg cttcaacgca tccctgggaa cgtatcacga tctcctgaaa atcattaaag
     5581 acaaggactt cctggacaat gaggagaacg aggacattct tgaggacatt gtcctcaccc
     5641 ttacgttgtt tgaagatagg gagatgattg aagaacgctt gaaaacttac gctcatctct
     5701 tcgacgacaa agtcatgaaa cagctcaaga ggcgccgata tacaggatgg gggcggctgt
     5761 caagaaaact gatcaatggg atccgagaca agcagagtgg aaagacaatc ctggattttc
     5821 ttaagtccga tggatttgcc aaccggaact tcatgcagtt gatccatgat gactctctca
     5881 cctttaagga ggacatccag aaagcacaag tttctggcca gggggacagt cttcacgagc
     5941 acatcgctaa tcttgcaggt agcccagcta tcaaaaaggg aatactgcag accgttaagg
     6001 tcgtggatga actcgtcaaa gtaatgggaa ggcataagcc cgagaatatc gttatcgaga
     6061 tggcccgaga gaaccaaact acccagaagg gacagaagaa cagtagggaa aggatgaaga
     6121 ggattgaaga gggtataaaa gaactggggt cccaaatcct taaggaacac ccagttgaaa
     6181 acacccagct tcagaatgag aagctctacc tgtactacct gcagaacggc agggacatgt
     6241 acgtggatca ggaactggac atcaatcggc tctccgacta cgacgtggat gccatcgtgc
     6301 cccagtcttt tctcaaagat gattctattg ataataaagt gttgacaaga tccgataaaa
     6361 atagagggaa gagtgataac gtcccctcag aagaagttgt caagaaaatg aaaaattatt
     6421 ggcggcagct gctgaacgcc aaactgatca cacaacggaa gttcgataat ctgactaagg
     6481 ctgaacgagg tggcctgtct gagttggata aagccggctt catcaaaagg cagcttgttg
     6541 agacacgcca gatcaccaag cacgtggccc aaattctcga ttcacgcatg aacaccaagt
     6601 acgatgaaaa tgacaaactg attcgagagg tgaaagttat tactctgaag tctaagctgg
     6661 tctcagattt cagaaaggac tttcagtttt ataaggtgag agagatcaac aattaccacc
     6721 atgcgcatga tgcctacctg aatgcagtgg taggcactgc acttatcaaa aaatatccca
     6781 agcttgaatc tgaatttgtt tacggagact ataaagtgta cgatgttagg aaaatgatcg
     6841 caaagtctga gcaggaaata ggcaaggcca ccgctaagta cttcttttac agcaatatta
     6901 tgaatttttt caagaccgag attacactgg ccaatggaga gattcggaag cgaccactta
     6961 tcgaaacaaa cggagaaaca ggagaaatcg tgtgggacaa gggtagggat ttcgcgacag
     7021 tccggaaggt cctgtccatg ccgcaggtga acatcgttaa aaagaccgaa gtacagaccg
     7081 gaggcttctc caaggaaagt atcctcccga aaaggaacag cgacaagctg atcgcacgca
     7141 aaaaagattg ggaccccaag aaatacggcg gattcgattc tcctacagtc gcttacagtg
     7201 tactggttgt ggccaaagtg gagaaaggga agtctaaaaa actcaaaagc gtcaaggaac
     7261 tgctgggcat cacaatcatg gagcgatcaa gcttcgaaaa aaaccccatc gactttctcg
     7321 aggcgaaagg atataaagag gtcaaaaaag acctcatcat taagcttccc aagtactctc
     7381 tctttgagct tgaaaacggc cggaaacgaa tgctcgctag tgcgggcgag ctgcagaaag
     7441 gtaacgagct ggcactgccc tctaaatacg ttaatttctt gtatctggcc agccactatg
     7501 aaaagctcaa agggtctccc gaagataatg agcagaagca gctgttcgtg gaacaacaca
     7561 aacactacct tgatgagatc atcgagcaaa taagcgaatt ctccaaaaga gtgatcctcg
     7621 ccgacgctaa cctcgataag gtgctttctg cttacaataa gcacagggat aagcccatca
     7681 gggagcaggc agaaaacatt atccacttgt ttactctgac caacttgggc gcgcctgcag
     7741 ccttcaagta cttcgacacc accatagaca gaaagcggta cacctctaca aaggaggtcc
     7801 tggacgccac actgattcat cagtcaatta cggggctcta tgaaacaaga atcgacctct
     7861 ctcagctcgg tggagacagc agggctgacc ccaagaagaa gaggaaggtg gctagcgatg
     7921 ctaagtcact gactgcctgg tcccggacac tggtgacctt caaggatgtg tttgtggact
     7981 tcaccaggga ggagtggaag ctgctggaca ctgctcagca gatcctgtac agaaatgtga
     8041 tgctggagaa ctataagaac ctggtttcct tgggttatca gcttactaag ccagatgtga
     8101 tcctccggtt ggagaaggga gaagagccct ggctggtgga gagagaaatt caccaagaga
     8161 cccatcctga ttcagagact gcatttgaaa tcaaatcatc agttccgaaa aagaaacgca
     8221 aagttgctag cgagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg
     8281 gccctggtac cgtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     8341 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     8401 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     8461 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     8521 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     8581 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     8641 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     8701 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     8761 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     8821 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     8881 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     8941 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     9001 acaagaccgg ttgataatag ataacttcgt atagcataca ttatacgaag ttatgaattc
     9061 gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     9121 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     9181 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     9241 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     9301 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     9361 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     9421 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     9481 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     9541 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     9601 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     9661 tccccgcatc gataccgtcg acctcgagac ctagaaaaac atggagcaat cacaagtagc
     9721 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg
     9781 ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat
     9841 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     9901 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac
     9961 tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta
    10021 ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac
    10081 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac
    10141 agccgcctag catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc
    10201 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
    10261 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
    10321 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagggcccgt
    10381 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
    10441 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
    10501 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
    10561 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
    10621 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
    10681 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
    10741 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
    10801 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
    10861 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
    10921 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
    10981 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
    11041 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
    11101 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
    11161 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
    11221 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
    11281 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
    11341 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
    11401 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
    11461 tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata
    11521 tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg
    11581 ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg
    11641 ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc
    11701 tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg
    11761 tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg
    11821 acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc
    11881 tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc
    11941 tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
    12001 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
    12061 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
    12121 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
    12181 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
    12241 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
    12301 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
    12361 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
    12421 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
    12481 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
    12541 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
    12601 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
    12661 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
    12721 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
    12781 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
    12841 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
    12901 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
    12961 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
    13021 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
    13081 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
    13141 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
    13201 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
    13261 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
    13321 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
    13381 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
    13441 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
    13501 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
    13561 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
    13621 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
    13681 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
    13741 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
    13801 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
    13861 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
    13921 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
    13981 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
    14041 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    14101 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    14161 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    14221 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    14281 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    14341 aacaaatagg ggttccgcgc acatttcccc g
//
