LOCUS       sequence-394117-       14378 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Derived from pLV hU6-sgRNA Nestin-dCas9-KRAB-T2a-GFP with KDR sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14378)
  AUTHORS   Dause TJ, Osap R, Kuwahara AA, Denninger JK, Kirby ED
  TITLE     Intracrine VEGF Signaling Is Required for Adult Hippocampal Neural 
            Stem Cell Maintenance and Vascular Proximity.
  JOURNAL   Mol Neurobiol. 2025 Mar 25. doi: 10.1007/s12035-025-04861-1.
  PUBMED    40131696
REFERENCE   2  (bases 1 to 14378)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Neurobiol. 2025 Mar 25. doi: 10.1007/s12035-025-04861-1."
COMMENT     Sequence Label: pLV hU6-VEGFR2#3 sgRNA Nestin-dCas9-KRAB-T2a-GFP
FEATURES             Location/Qualifiers
     source          1..14378
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(39..58)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        230..609
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        611..809
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     764..784
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             827..1007
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1054..1179
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1672..1905
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2090..2134
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2432..2549
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2593..2841
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2593..2613
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2764..2783
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2855..2930
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     regulatory      3646..3655
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3655..3720
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3727..3747
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3757..3783,3784..3786,3787..6270,6271..6276,6277..6342,
                     6343..6345,6346..7860)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(3757..3783,3784..3786,3787..6273,6274..6276,
                     6277..7860)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3757..7860
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7873..7893
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7927..8121
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQILYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     CDS             8188..8208
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8215..8268
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(8275..8277,8278..8988)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(8275..8277,8278..8988)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(8317..8338)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8578..8597)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8925..8946
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(9004..9037)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    9062..9650
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9115..9135)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9533..9544)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9653..9669)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9654..9670)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10175..10355
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10381..10398)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10387..10611
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10657..11085
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10744..10763)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10954..10975
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11094..11114)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11099..11428
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11279..11414
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11341..11360
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        11476..11523
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             11542..11916
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    12046..12167
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12083..12102)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12137..12156
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12216..12232)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12216..12232)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12229..12251)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12240..12256
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12264..12270,12271..12288,12289..12294))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12309..12330
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12447..12464)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12618..13206)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12698..12717)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13377..14168,14169..14237))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14000..14019
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14238..14342)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
       61 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
      121 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
      181 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
      241 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      301 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      361 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      421 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      481 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      541 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      601 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      661 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      721 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      781 cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt
      841 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
      901 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
      961 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     1021 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     1081 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     1141 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     1201 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     1261 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     1321 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     1381 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     1441 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     1501 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     1561 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     1621 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     1681 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     1741 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     1801 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     1861 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     1921 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     1981 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     2041 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     2101 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     2161 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     2221 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     2281 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     2341 agagagagac agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat
     2401 tctgcagaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
     2461 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     2521 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag
     2581 tttggttaat tagagggcct atttcccatg attccttcat atttgcatat acgatacaag
     2641 gctgttagag agataattgg aattaatttg actgtaaaca caaagatatt agtacaaaat
     2701 acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa
     2761 atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc
     2821 ttgtggaaag gacgagatcg cgcggaccgc taccgtttta gagctagaaa tagcaagtta
     2881 aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc ttttttgttt
     2941 tttaattaat taacccgtgt cggctccaga tctgatccct gaagagtttg tgatcctgag
     3001 atgagggctt tagccccagt cagtcctctg aggggaaggg tccaggcagc tctgaggaat
     3061 gtaaccactg gcgtttgagg tctgaaaagg atttggagaa ggggagctga attcatttgc
     3121 ttttgtctgt taccagctct gggggcagag agagagccat cccctgggaa cagcctgaga
     3181 attcccactt cccctgagga gccctccctt cttaggccct ccagatggta gtgtggacaa
     3241 aaggcaataa ttagcatgag aatcggcctc cctcccagag gatgaggtca tcggccttgg
     3301 ccttgggtgg ggaggcggag actgatctga ggagtctgat ataagtgtta gcaattcatt
     3361 tggccctgcc tccgactgtg ggaatctgca tgtggggtct ccctgtgtct caaatatggg
     3421 ttggctaagt atatatctgt gggtatatga ctgtgtggct tttatatgac aatggtcaca
     3481 atagagattg atcctgcagt ggcaggacat gctacctcag ctggagctga ccctatctcc
     3541 ccactcccca ccaggactct gctggaggct gagaactctc ggttgcagac acctggacga
     3601 ggttcccagg cttaatcgaa gcttgggctg caggtcgact ctagagccac catggactac
     3661 aaagaccatg acggtgatta taaagatcat gacatcgatt acaaggatga cgatgacaag
     3721 atggccccca agaagaagag gaaggtgggc cgcggaatgg acaagaagta ctccattggg
     3781 ctcgccatcg gcacaaacag cgtcggctgg gccgtcatta cggacgagta caaggtgccg
     3841 agcaaaaaat tcaaagttct gggcaatacc gatcgccaca gcataaagaa gaacctcatt
     3901 ggcgccctcc tgttcgactc cggggaaacc gccgaagcca cgcggctcaa aagaacagca
     3961 cggcgcagat atacccgcag aaagaatcgg atctgctacc tgcaggagat ctttagtaat
     4021 gagatggcta aggtggatga ctctttcttc cataggctgg aggagtcctt tttggtggag
     4081 gaggataaaa agcacgagcg ccacccaatc tttggcaata tcgtggacga ggtggcgtac
     4141 catgaaaagt acccaaccat atatcatctg aggaagaagc ttgtagacag tactgataag
     4201 gctgacttgc ggttgatcta tctcgcgctg gcgcatatga tcaaatttcg gggacacttc
     4261 ctcatcgagg gggacctgaa cccagacaac agcgatgtcg acaaactctt tatccaactg
     4321 gttcagactt acaatcagct tttcgaagag aacccgatca acgcatccgg agttgacgcc
     4381 aaagcaatcc tgagcgctag gctgtccaaa tcccggcggc tcgaaaacct catcgcacag
     4441 ctccctgggg agaagaagaa cggcctgttt ggtaatctta tcgccctgtc actcgggctg
     4501 acccccaact ttaaatctaa cttcgacctg gccgaagatg ccaagcttca actgagcaaa
     4561 gacacctacg atgatgatct cgacaatctg ctggcccaga tcggcgacca gtacgcagac
     4621 ctttttttgg cggcaaagaa cctgtcagac gccattctgc tgagtgatat tctgcgagtg
     4681 aacacggaga tcaccaaagc tccgctgagc gctagtatga tcaagcgcta tgatgagcac
     4741 caccaagact tgactttgct gaaggccctt gtcagacagc aactgcctga gaagtacaag
     4801 gaaattttct tcgatcagtc taaaaatggc tacgccggat acattgacgg cggagcaagc
     4861 caggaggaat tttacaaatt tattaagccc atcttggaaa aaatggacgg caccgaggag
     4921 ctgctggtaa agcttaacag agaagatctg ttgcgcaaac agcgcacttt cgacaatgga
     4981 agcatccccc accagattca cctgggcgaa ctgcacgcta tcctcaggcg gcaagaggat
     5041 ttctacccct ttttgaaaga taacagggaa aagattgaga aaatcctcac atttcggata
     5101 ccctactatg taggccccct cgcccgggga aattccagat tcgcgtggat gactcgcaaa
     5161 tcagaagaga ccatcactcc ctggaacttc gaggaagtcg tggataaggg ggcctctgcc
     5221 cagtccttca tcgaaaggat gactaacttt gataaaaatc tgcctaacga aaaggtgctt
     5281 cctaaacact ctctgctgta cgagtacttc acagtttata acgagctcac caaggtcaaa
     5341 tacgtcacag aagggatgag aaagccagca ttcctgtctg gagagcagaa gaaagctatc
     5401 gtggacctcc tcttcaagac gaaccggaaa gttaccgtga aacagctcaa agaagactat
     5461 ttcaaaaaga ttgaatgttt cgactctgtt gaaatcagcg gagtggagga tcgcttcaac
     5521 gcatccctgg gaacgtatca cgatctcctg aaaatcatta aagacaagga cttcctggac
     5581 aatgaggaga acgaggacat tcttgaggac attgtcctca cccttacgtt gtttgaagat
     5641 agggagatga ttgaagaacg cttgaaaact tacgctcatc tcttcgacga caaagtcatg
     5701 aaacagctca agaggcgccg atatacagga tgggggcggc tgtcaagaaa actgatcaat
     5761 gggatccgag acaagcagag tggaaagaca atcctggatt ttcttaagtc cgatggattt
     5821 gccaaccgga acttcatgca gttgatccat gatgactctc tcacctttaa ggaggacatc
     5881 cagaaagcac aagtttctgg ccagggggac agtcttcacg agcacatcgc taatcttgca
     5941 ggtagcccag ctatcaaaaa gggaatactg cagaccgtta aggtcgtgga tgaactcgtc
     6001 aaagtaatgg gaaggcataa gcccgagaat atcgttatcg agatggcccg agagaaccaa
     6061 actacccaga agggacagaa gaacagtagg gaaaggatga agaggattga agagggtata
     6121 aaagaactgg ggtcccaaat ccttaaggaa cacccagttg aaaacaccca gcttcagaat
     6181 gagaagctct acctgtacta cctgcagaac ggcagggaca tgtacgtgga tcaggaactg
     6241 gacatcaatc ggctctccga ctacgacgtg gatgccatcg tgccccagtc ttttctcaaa
     6301 gatgattcta ttgataataa agtgttgaca agatccgata aaaatagagg gaagagtgat
     6361 aacgtcccct cagaagaagt tgtcaagaaa atgaaaaatt attggcggca gctgctgaac
     6421 gccaaactga tcacacaacg gaagttcgat aatctgacta aggctgaacg aggtggcctg
     6481 tctgagttgg ataaagccgg cttcatcaaa aggcagcttg ttgagacacg ccagatcacc
     6541 aagcacgtgg cccaaattct cgattcacgc atgaacacca agtacgatga aaatgacaaa
     6601 ctgattcgag aggtgaaagt tattactctg aagtctaagc tggtctcaga tttcagaaag
     6661 gactttcagt tttataaggt gagagagatc aacaattacc accatgcgca tgatgcctac
     6721 ctgaatgcag tggtaggcac tgcacttatc aaaaaatatc ccaagcttga atctgaattt
     6781 gtttacggag actataaagt gtacgatgtt aggaaaatga tcgcaaagtc tgagcaggaa
     6841 ataggcaagg ccaccgctaa gtacttcttt tacagcaata ttatgaattt tttcaagacc
     6901 gagattacac tggccaatgg agagattcgg aagcgaccac ttatcgaaac aaacggagaa
     6961 acaggagaaa tcgtgtggga caagggtagg gatttcgcga cagtccggaa ggtcctgtcc
     7021 atgccgcagg tgaacatcgt taaaaagacc gaagtacaga ccggaggctt ctccaaggaa
     7081 agtatcctcc cgaaaaggaa cagcgacaag ctgatcgcac gcaaaaaaga ttgggacccc
     7141 aagaaatacg gcggattcga ttctcctaca gtcgcttaca gtgtactggt tgtggccaaa
     7201 gtggagaaag ggaagtctaa aaaactcaaa agcgtcaagg aactgctggg catcacaatc
     7261 atggagcgat caagcttcga aaaaaacccc atcgactttc tcgaggcgaa aggatataaa
     7321 gaggtcaaaa aagacctcat cattaagctt cccaagtact ctctctttga gcttgaaaac
     7381 ggccggaaac gaatgctcgc tagtgcgggc gagctgcaga aaggtaacga gctggcactg
     7441 ccctctaaat acgttaattt cttgtatctg gccagccact atgaaaagct caaagggtct
     7501 cccgaagata atgagcagaa gcagctgttc gtggaacaac acaaacacta ccttgatgag
     7561 atcatcgagc aaataagcga attctccaaa agagtgatcc tcgccgacgc taacctcgat
     7621 aaggtgcttt ctgcttacaa taagcacagg gataagccca tcagggagca ggcagaaaac
     7681 attatccact tgtttactct gaccaacttg ggcgcgcctg cagccttcaa gtacttcgac
     7741 accaccatag acagaaagcg gtacacctct acaaaggagg tcctggacgc cacactgatt
     7801 catcagtcaa ttacggggct ctatgaaaca agaatcgacc tctctcagct cggtggagac
     7861 agcagggctg accccaagaa gaagaggaag gtggctagcg atgctaagtc actgactgcc
     7921 tggtcccgga cactggtgac cttcaaggat gtgtttgtgg acttcaccag ggaggagtgg
     7981 aagctgctgg acactgctca gcagatcctg tacagaaatg tgatgctgga gaactataag
     8041 aacctggttt ccttgggtta tcagcttact aagccagatg tgatcctccg gttggagaag
     8101 ggagaagagc cctggctggt ggagagagaa attcaccaag agacccatcc tgattcagag
     8161 actgcatttg aaatcaaatc atcagttccg aaaaagaaac gcaaagttgc tagcgagggc
     8221 agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctgg taccgtgagc
     8281 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     8341 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     8401 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     8461 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     8521 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     8581 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     8641 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     8701 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     8761 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     8821 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     8881 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     8941 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagac cggttgataa
     9001 tagataactt cgtatagcat acattatacg aagttatgaa ttcgatatca agcttatcga
     9061 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     9121 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     9181 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     9241 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     9301 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     9361 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     9421 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     9481 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     9541 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     9601 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
     9661 tcgacctcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa
     9721 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc
     9781 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     9841 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct
     9901 gtggatctac cacacacaag gctacttccc tgattggcag aactacacac cagggccagg
     9961 gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa
    10021 ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg
    10081 gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca
    10141 tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag accagatctg
    10201 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
    10261 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
    10321 cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag
    10381 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
    10441 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
    10501 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
    10561 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
    10621 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
    10681 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
    10741 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
    10801 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
    10861 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
    10921 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
    10981 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
    11041 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
    11101 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
    11161 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
    11221 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
    11281 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
    11341 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
    11401 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
    11461 atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac
    11521 gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg
    11581 cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc
    11641 gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc
    11701 caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag
    11761 ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc
    11821 atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc
    11881 aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc
    11941 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
    12001 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
    12061 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
    12121 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
    12181 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
    12241 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
    12301 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
    12361 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
    12421 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
    12481 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
    12541 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
    12601 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
    12661 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
    12721 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
    12781 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
    12841 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
    12901 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
    12961 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
    13021 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
    13081 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
    13141 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    13201 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    13261 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
    13321 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
    13381 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
    13441 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
    13501 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
    13561 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
    13621 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
    13681 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
    13741 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    13801 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    13861 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    13921 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    13981 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    14041 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    14101 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    14161 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    14221 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    14281 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    14341 cgcacatttc cccgaaaagt gccacctgac gtcgacgg
//
