LOCUS       sequence-394124-       14769 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA binary vector to express pco-nCasphi driven by UBQ10 gene 
            promoter, and the AtPDS3 guide RNA 10 driven by U6 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14769)
  AUTHORS   Li Z, Zhong Z, Wu Z, Pausch P, Al-Shayeb B, Amerasekera J, Doudna 
            JA, Jacobsen SE
  TITLE     Genome editing in plants using the compact editor CasPhi.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Jan 24;120(4):e2216822120. doi: 
            10.1073/pnas.2216822120. Epub 2023 Jan 18.
  PUBMED    36652483
REFERENCE   2  (bases 1 to 14769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-01-24"; volume: "120(4):e2216822120. 
            doi"; pages: " 10.1073/pnas.2216822120. Epub 2023 Jan 18"
COMMENT     Sequence Label: pC1300_pUB10_pco-nCASphi_E9t_V2_U6_AtPDS3_gRNA10
FEATURES             Location/Qualifiers
     source          1..14769
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4..20)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4..20)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(17..39)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    28..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(52..58,59..76,77..82))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    97..118
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        309..985
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     922..942
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1052..2077
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    2118..2292
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    2370..2394
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2819..3613
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      3700..4288
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4189..4208
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4442..4459
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4474..4614
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4540..4559)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4700..4722
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4958..5152
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(5218..6291)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(6720..7349)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    8650..8674
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     8862..8884
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8876..8893
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8877..8893
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     intron          12499..12687
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     CDS             14006..14026
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             14033..14053
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             14072..14119
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
ORIGIN
        1 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
       61 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
      121 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
      181 aatcggccaa cgcgcgggga gaggcggttt gcgtattggc tagagcagct tgccaacatg
      241 gtggagcacg acactctcgt ctactccaag aatatcaaag atacagtctc agaagaccaa
      301 agggctattg agacttttca acaaagggta atatcgggaa acctcctcgg attccattgc
      361 ccagctatct gtcacttcat caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc
      421 catcattgcg ataaaggaaa ggctatcgtt caagatgcct ctgccgacag tggtcccaaa
      481 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
      541 aagcaagtgg attgatgtga acatggtgga gcacgacact ctcgtctact ccaagaatat
      601 caaagataca gtctcagaag accaaagggc tattgagact tttcaacaaa gggtaatatc
      661 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcaaaa ggacagtaga
      721 aaaggaaggt ggcacctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga
      781 tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa
      841 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt
      901 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc
      961 atttcatttg gagaggacac gctgaaatca ccagtctctc tctacaaatc tatctctctc
     1021 gagctttcgc agatccgggg ggcaatgaga tatgaaaaag cctgaactca ccgcgacgtc
     1081 tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga
     1141 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
     1201 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
     1261 cgcgctcccg attccggaag tgcttgacat tggggagttt agcgagagcc tgacctattg
     1321 catctcccgc cgttcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
     1381 tgttctacaa ccggtcgcgg aggctatgga tgcgatcgct gcggccgatc ttagccagac
     1441 gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt
     1501 catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt
     1561 cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga
     1621 agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg
     1681 cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc
     1741 caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga
     1801 gcggaggcat ccggagcttg caggatcgcc acgactccgg gcgtatatgc tccgcattgg
     1861 tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca
     1921 gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc
     1981 ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa
     2041 ccgacgcccc agcactcgtc cgagggcaaa gaaatagagt agatgccgac cgggatctgt
     2101 cgatcgacaa gctcgagttt ctccataata atgtgtgagt agttcccaga taagggaatt
     2161 agggttccta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta gtatgtattt
     2221 gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa atccagtact
     2281 aaaatccaga tcccccgaat taattcggcg ttaattcagt acattaaaaa cgtccgcaat
     2341 gtgttattaa gttgtctaag cgtcaatttg tttacaccac aatatatcct gccaccagcc
     2401 agccaacagc tccccgaccg gcagctcggc acaaaatcac cactcgatac aggcagccca
     2461 tcagtccggg acggcgtcag cgggagagcc gttgtaaggc ggcagacttt gctcatgtta
     2521 ccgatgctat tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc
     2581 ggagggtagc atgttgattg taacgatgac agagcgttgc tgcctgtgat caccgcggtt
     2641 tcaaaatcgg ctccgtcgat actatgttat acgccaactt tgaaaacaac tttgaaaaag
     2701 ctgttttctg gtatttaagg ttttagaatg caaggaacag tgaattggag ttcgtcttgt
     2761 tataattagc ttcttggggt atctttaaat actgtagaaa agaggaagga aataataaat
     2821 ggctaaaatg agaatatcac cggaattgaa aaaactgatc gaaaaatacc gctgcgtaaa
     2881 agatacggaa ggaatgtctc ctgctaaggt atataagctg gtgggagaaa atgaaaacct
     2941 atatttaaaa atgacggaca gccggtataa agggaccacc tatgatgtgg aacgggaaaa
     3001 ggacatgatg ctatggctgg aaggaaagct gcctgttcca aaggtcctgc actttgaacg
     3061 gcatgatggc tggagcaatc tgctcatgag tgaggccgat ggcgtccttt gctcggaaga
     3121 gtatgaagat gaacaaagcc ctgaaaagat tatcgagctg tatgcggagt gcatcaggct
     3181 ctttcactcc atcgacatat cggattgtcc ctatacgaat agcttagaca gccgcttagc
     3241 cgaattggat tacttactga ataacgatct ggccgatgtg gattgcgaaa actgggaaga
     3301 agacactcca tttaaagatc cgcgcgagct gtatgatttt ttaaagacgg aaaagcccga
     3361 agaggaactt gtcttttccc acggcgacct gggagacagc aacatctttg tgaaagatgg
     3421 caaagtaagt ggctttattg atcttgggag aagcggcagg gcggacaagt ggtatgacat
     3481 tgccttctgc gtccggtcga tcagggagga tatcggggaa gaacagtatg tcgagctatt
     3541 ttttgactta ctggggatca agcctgattg ggagaaaata aaatattata ttttactgga
     3601 tgaattgttt tagtacctag aatgcatgac caaaatccct taacgtgagt tttcgttcca
     3661 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     3721 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     3781 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     3841 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     3901 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     3961 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     4021 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     4081 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     4141 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     4201 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     4261 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     4321 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     4381 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     4441 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     4501 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
     4561 atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
     4621 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     4681 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     4741 aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg tcgagtggcg acggcgcggc
     4801 ttgtccgcgc cctggtagat tgcctggccg taggccagcc atttttgagc ggccagcggc
     4861 cgcgataggc cgacgcgaag cggcggggcg tagggagcgc agcgaccgaa gggtaggcgc
     4921 tttttgcagc tcttcggctg tgcgctggcc agacagttat gcacaggcca ggcgggtttt
     4981 aagagtttta ataagtttta aagagtttta ggcggaaaaa tcgccttttt tctcttttat
     5041 atcagtcact tacatgtgtg accggttccc aatgtacggc tttgggttcc caatgtacgg
     5101 gttccggttc ccaatgtacg gctttgggtt cccaatgtac gtgctatcca caggaaagag
     5161 accttttcga cctttttccc ctgctagggc aatttgccct agcatctgct ccgtacatta
     5221 ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag cgcatgacta ggatcgggcc
     5281 agcctgcccc gcctcctcct tcaaatcgta ctccggcagg tcatttgacc cgatcagctt
     5341 gcgcacggtg aaacagaact tcttgaactc tccggcgctg ccactgcgtt cgtagatcgt
     5401 cttgaacaac catctggctt ctgccttgcc tgcggcgcgg cgtgccaggc ggtagagaaa
     5461 acggccgatg ccgggatcga tcaaaaagta atcggggtga accgtcagca cgtccgggtt
     5521 cttgccttct gtgatctcgc ggtacatcca atcagctagc tcgatctcga tgtactccgg
     5581 ccgcccggtt tcgctcttta cgatcttgta gcggctaatc aaggcttcac cctcggatac
     5641 cgtcaccagg cggccgttct tggccttctt cgtacgctgc atggcaacgt gcgtggtgtt
     5701 taaccgaatg caggtttcta ccaggtcgtc tttctgcttt ccgccatcgg ctcgccggca
     5761 gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg ccgggcttgt ctcccttccc
     5821 ttcccggtat cggttcatgg attcggttag atgggaaacc gccatcagta ccaggtcgta
     5881 atcccacaca ctggccatgc cggccggccc tgcggaaacc tctacgtgcc cgtctggaag
     5941 ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc gacagacgga aaacggccac
     6001 gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag agcatcggaa cgaaaaaatc
     6061 tggttgctcg tcgcccttgg gcggcttcct aatcgacggc gcaccggctg ccggcggttg
     6121 ccgggattct ttgcggattc gatcagcggc cgcttgccac gattcaccgg ggcgtgcttc
     6181 tgcctcgatg cgttgccgct gggcggcctg cgcggccttc aacttctcca ccaggtcatc
     6241 acccagcgcc gcgccgattt gtaccgggcc ggatggtttg cgaccgctca cgccgattcc
     6301 tcgggcttgg gggttccagt gccattgcag ggccggcagg caacccagcc gcttacgcct
     6361 ggccaaccgc ccgttcctcc acacatgggg cattccacgg cgtcggtgcc tggttgttct
     6421 tgattttcca tgccgcctcc tttagccgct aaaattcatc tactcattta ttcatttgct
     6481 catttactct ggtagctgcg cgatgtattc agatagcagc tcggtaatgg tcttgccttg
     6541 gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc ggcaactgaa agttgacccg
     6601 cttcatggct ggcgtgtctg ccaggctggc caacgttgca gccttgctgc tgcgtgcgct
     6661 cggacggccg gcacttagcg tgtttgtgct tttgctcatt ttctctttac ctcattaact
     6721 caaatgagtt ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc agcgtcgccc
     6781 tcgggttctg attcaagaac ggttgtgccg gcggcggcag tgcctgggta gctcacgcgc
     6841 tgcgtgatac gggactcaag aatgggcagc tcgtacccgg ccagcgcctc ggcaacctca
     6901 ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg tagccttcca
     6961 tccgtgacct caatgcgctg cttaaccagc tccaccaggt cggcggtggc ccatatgtcg
     7021 taagggcttg gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc ggacacagcc
     7081 aagtccgccg cctggggcgc tccgtcgatc actacgaagt cgcgccggcc gatggccttc
     7141 acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg atcttcccgc
     7201 acggccgccc aatcgcgggc actgccctgg ggatcggaat cgactaacag aacatcggcc
     7261 ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc tgacccgcct
     7321 ttctggttaa gtacagcgat aaccttcatg cgttcccctt gcgtatttgt ttatttactc
     7381 atcgcatcat atacgcagcg accgcatgac gcaagctgtt ttactcaaat acacatcacc
     7441 tttttagacg gcggcgctcg gtttcttcag cggccaagct ggccggccag gccgccagct
     7501 tggcatcaga caaaccggcc aggatttcat gcagccgcac ggttgagacg tgcgcgggcg
     7561 gctcgaacac gtacccggcc gcgatcatct ccgcctcgat ctcttcggta atgaaaaacg
     7621 gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt cattctcggc
     7681 ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc gccgcctggc
     7741 ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg agcgatgcac
     7801 gccaagcagt gcagccgcct ctttcacggt gcggccttcc tggtcgatca gctcgcgggc
     7861 gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca aacttcacgc ctcgggcctt
     7921 ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg gaacgctcga actcggcaat
     7981 gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg cccacggctc
     8041 tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc tcggcaatgt ccagtaggtc
     8101 gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc acaacgtcgc cagggcgtag
     8161 gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg gtgccggtga tcttctcgga
     8221 aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt tggttggtca agtcctggtc
     8281 gtcggtgctg acgcgggcat agcccagcag gccagcggcg gcgctcttgt tcatggcgta
     8341 atgtctccgg ttctagtcgc aagtattcta ctttatgcga ctaaaacacg cgacaagaaa
     8401 acgccaggaa aagggcaggg cggcagcctg tcgcgtaact taggacttgt gcgacatgtc
     8461 gttttcagaa gacggctgca ctgaacgtca gaagccgact gcactatagc agcggagggg
     8521 ttggatcaaa gtactttgat cccgagggga accctgtggt tggcatgcac atacaaatgg
     8581 acgaacggat aaaccttttc acgccctttt aaatatccga ttattctaat aaacgctctt
     8641 ttctcttagg tttacccgcc aatatatcct gtcaaacact gatagtttaa actgaaggcg
     8701 ggaaacgaca atctgatcca agctcaagct gctctagcat tcgccattca ggctgcgcaa
     8761 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     8821 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
     8881 aacgacggcc agtgccaagc ttgcatgcct gcaggtcgac tctagatcac tagtgatcag
     8941 atgcagagag actaacctca gataggcgac ttcaaaaccc cccgaaaatt tgacaatgga
     9001 gcttgaaaag aatggcaaaa aaaaattggc tggattgtca actggtcccc tcgtgagggg
     9061 caatcgttga gcgttccgac caatcactac ttcgactcta gctgtatata aactcagctt
     9121 cgttttctta tctaagcgat gtgggacttt tgaagattgt tttcaactta aatgggccta
     9181 tataagaaat actattgttc tttcccatat aaatgggcct gcttctcttc tttcagattc
     9241 ccaggggcct tttgaagatt atcttcatat cttaagaatg aagatgtttt attcaatcaa
     9301 attcttgaag gttcgatgcc taatcattct aatcctggga caaactatga aacaagatac
     9361 aaaaactccg aatggaaagt taaaaagaag aaaacgaaag ctacggttca agaaaatgta
     9421 agctgataaa caaaaaaaaa ctgtatgaac gaagaagaag aaaaaaagct aagaagaaat
     9481 gatgtattgt gcggaaggca agtcgagttt ccgttgttca acgaagcttc tagtatccta
     9541 ggaaggtacc agtctagctc aacagagctt ttaacccaaa ttggtacaat agaatacaac
     9601 tttagatcat aattctcaaa agaaagagat tccttagcta ttctatctgc cactccattt
     9661 ccttctcggc ttgtatgcac aagcataaaa tcctcaaact tgctaagtag atactttatg
     9721 tcttggataa ttggattgag acttgacaag cataactttc atgtaaccaa agacacaagt
     9781 tgctgagaat ccacctcaaa aatgatcttc ctataattga atcgggataa tgacagcaca
     9841 gcccatctaa gagcctccac ttctacttcc agcacgcttc ttacttttac cacagctctt
     9901 gcacctaacc ataacacctt ccctgtatga tcgcgaagca cccaccctaa gccacatttt
     9961 aatccttctg ttggccatgc cccatcaaag ttgcacttaa cccaagattg tggtggagct
    10021 tcccatgttt ctcgtctgtc ccgacggtgt tgtggttggt gctttcctta cattctgagc
    10081 ctctttcctt ctaatccact catctgcatc ttcttgtgtc cttactaata cctcattggt
    10141 tccaaattcc ctccctttaa gcaccagctc gtttctgttc ttccacagcc tcccaagtat
    10201 ccaagggact aaagcctcca cattcttcag atcaggatat tcttgtttaa gatgttgaac
    10261 tctatggagg tttgtatgaa ctgatgatct aggaccggat aagttccctt cttcatagcg
    10321 aacttattca aagaatgttt tgtgtatcat tcttgttaca ttgttattaa tgaaaaaata
    10381 ttattggtca ttggactgaa cacgagtgtt aaatatggac caggccccaa ataagatcca
    10441 ttgatatatg aattaaataa caagaataaa tcgagtcacc aaaccacttg ccttttttaa
    10501 cgagacttgt tcaccaactt gatacaaaag tcattatcct atgcaaatca ataatcatac
    10561 aaaaatatcc aataacacta aaaaattaaa agaaatggat aatttcacaa tatgttatac
    10621 gataaagaag ttacttttcc aagaaattca ctgattttat aagcccactt gcattagata
    10681 aatggcaaaa aaaaacaaaa aggaaaagaa ataaagcacg aagaattcta gaaaatacga
    10741 aatacgcttc aatgcagtgg gacccacggt tcaattattg ccaattttca gctccaccgt
    10801 atatttaaaa aataaaacga taatgctaaa aaaatataaa tcgtaacgat cgttaaatct
    10861 caacggctgg atcttatgac gaccgttaga aattgtggtt gtcgacgagt cagtaataaa
    10921 cggcgtcaaa gtggttgcag ccggcacaca cgagtcgtgt ttatcaactc aaagcacaaa
    10981 tacttttcct caacctaaaa ataaggcaat tagccaaaaa caactttgcg tgtaaacaac
    11041 gctcaataca cgtgtcattt tattattagc tattgcttca ccgccttagc tttctcgtga
    11101 cctagtcgtc ctcgtctttt cttcttcttc ttctataaaa caatacccaa agagctcttc
    11161 ttcttcacaa ttcagatttc aatttctcaa aatcttaaaa actttctctc aattctctct
    11221 accgtgatca aggtaaattt ctgtgttcct tattctctca aaatcttcga ttttgttttc
    11281 gttcgatccc aatttcgtat atgttctttg gtttagattc tgttaatctt agatcgaaga
    11341 cgattttctg ggtttgatcg ttagatatca tcttaattct cgattagggt ttcatagata
    11401 tcatccgatt tgttcaaata atttgagttt tgtcgaataa ttactcttcg atttgtgatt
    11461 tctatctaga tctggtgtta gtttctagtt tgtgcgatcg aatttgtcga ttaatctgag
    11521 tttttctgat taacagatgc cgaagcccgc cgtcgaatca gagttttcca aagtcctcaa
    11581 gaaacacttt cctggggagc gttttaggtc tagctatatg aagagggggg gtaaaattct
    11641 ggcagcacaa ggcgaggaag ctgtagtggc gtacttgcag ggaaagagtg aggaggaacc
    11701 gccgaatttt cagccgccgg cgaagtgcca cgtggtcacc aaaagcaggg atttcgcaga
    11761 atggcccata atgaaagcct ctgaagccat acagaggtac atctatgcgc tcagcactac
    11821 agagcgagct gcctgcaaac cgggtaagag ctcagaaagt cacgcggcct ggttcgcggc
    11881 tacaggggtg agcaatcacg gctattctca tgtacaaggt cttaacctga tctttgacca
    11941 cacgctagga cgatacgatg gcgttttaaa gaaagtacag cttcgaaatg agaaggcccg
    12001 agctagattg gctgcaataa acgccgctcg agctgcagct ggattgccgg aaatcaaggc
    12061 cgaggaggag gaggtagcta caaatgaaac aggtcatcta ctacagccgc caggcataaa
    12121 cccatcattc tacgtctacc agaccatatc tccgcaggct taccgaccaa gggacgaaat
    12181 agtgttacca cccgagtacg ccggttacgt cagggatccg aacgctccga ttccactggg
    12241 cgtggtcagg aaccgttgtg acatacagaa gggttgcccg ggatatatac ccgagtggca
    12301 gagggaagct ggtacggcaa ttagtcccaa gacaggaaaa gcagtgacgg ttccaggact
    12361 tagcccgaag aagaataaac gtatgcgtag gtactggagg tcagaaaagg agaaggctca
    12421 agatgcactt ctcgtaactg taaggatagg taccgattgg gtagtcatag acgtaagagg
    12481 attactaaga aacgccaggt aagtttctgc ttctaccttt gatatatata taataattat
    12541 cattaattag tagtaatata atatttcaaa tatttttttc aaaataaaag aatgtagtat
    12601 atagcaattg cttttctgta gtttataagt gtgtatattt taatttataa cttttctaat
    12661 atatgaccaa aatttgttga tgtgcaggtg gaggactatt gctcccaagg acataagttt
    12721 aaatgcactt ctagatttat ttaccggtga tccagtcatc gatgtcagac gaaacatcgt
    12781 gaccttcacc tataccttgg acgcttgcgg aacttatgct agaaaatgga ctctcaaggg
    12841 aaaacagaca aaagcaacct tagataaact gacagcgaca caaactgtgg ccttagttgc
    12901 tatagatctg ggacaaacaa acccaattag cgcgggtatc agtcgtgtca cacaggagaa
    12961 cggggccctc cagtgcgaac cgcttgatcg ttttacattg cctgatgacc ttttgaaaga
    13021 tatttctgcg taccgaattg catgggaccg taacgaggag gaactcaggg ccagatccgt
    13081 tgaggcactc ccagaggcac aacaagcaga ggtcagagct ctggacgggg tctccaaaga
    13141 gaccgcgcgt acacagttgt gcgcggactt cggtctggac ccaaagcgac taccgtggga
    13201 taaaatgagt agcaatacca cgttcataag cgaggcgctc ctttccaaca gcgtatcccg
    13261 tgaccaagta ttctttaccc cggccccaaa gaaaggagcc aagaagaaag caccggtgga
    13321 agtgatgcga aaagacagga catgggcgcg agcgtacaaa ccacgactct cagtagaagc
    13381 acaaaagttg aaaaatgagg ctctttgggc tttgaagcgt acctctccag aatatctaaa
    13441 gttgtcacga cgtaaagaag aattgtgtag gaggtccatt aattacgtga tagaaaaaac
    13501 tagaaggcga acccaatgcc aaattgtgat ccccgttata gaagatctaa atgtccgatt
    13561 tttccacggg tctggcaaac gactcccggg ctgggataac ttcttcacgg caaagaagga
    13621 aaaccgatgg tttatccaag ggctacataa ggcgttttct gacttaagga cccaccgatc
    13681 cttctacgtg ttcgaggtgc gtccagagag aacatcaatc acctgtccga aatgcgggca
    13741 ctgtgaagtg gggaacaggg atggagaagc ctttcagtgt ctcagttgcg gtaagacatg
    13801 caacgcagat ctggacgtag cgacacataa cttgactcaa gtggcgctca ccggcaaaac
    13861 aatgccgaag agagaggagc ctagagatgc acaagggaca gcccccgcga gaaagacgaa
    13921 gaaagctagc aagtcaaagg cacccccggc tgaacgtgag gatcagactc ccgctcaaga
    13981 gccgtcccaa acgtccggat ccggaccgaa gaaaaagcga aaggtagagg atcctaaaaa
    14041 gaagcgtaaa gtctccttgg gttctggctc cgactataag gatgacgatg acaaagacta
    14101 taaggatgac gacgacaagt gaagagcttt cgttcgtatc atcggtttcg acaacgttcg
    14161 tcaagttcaa tgcatcagtt tcattgcgca cacaccagaa tcctactgag tttgagtatt
    14221 atggcattgg gaaaactgtt tttcttgtac catttgttgt gcttgtaatt tactgtgttt
    14281 tttattcggt tttcgctatc gaactgtgaa atggaaatgg atggagaaga gttaatgaat
    14341 gatatggtcc ttttgttcat tctcaaatta atattatttg ttttttctct tatttgttgt
    14401 gtgttgaatt tgaaattata agagatatgc aaacattttg ttttgagtaa aaatgtgtca
    14461 aatcgtggcc tctaatgacc gaagttaata tgaggagtaa aacacttgta gttgtaccat
    14521 tatgcttatt cactaggcaa caaatatatt ttcagaccta gaaaagctgc aaatgttact
    14581 gaatacaagt atgtcctctt gtgttttaga catttatgaa ctttccttta tgtaattttc
    14641 cagaatcctt gtcagattct aatcattgct ttataattat agttatactc atggatttgt
    14701 agttgagtat gaaaatattt tttaatgcat tttatgactt gccaattgat tgacaacgaa
    14761 ttcgtaatc
//
