LOCUS       sequence-394139-        9604 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses kinase-inactive VRK1 (K179E).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9604)
  AUTHORS   So J, Mabe NW, Englinger B, Chow KH, Moyer SM, Yerrum S, Trissal MC,
            Marques JG, Kwon JJ, Shim B, Pal S, Panditharatna E, Quinn T, 
            Schaefer DA, Jeong D, Mayhew DL, Hwang J, Beroukhim R, Ligon KL, 
            Stegmaier K, Filbin MG, Hahn WC
  TITLE     VRK1 as a synthetic lethal target in VRK2 promoter-methylated 
            cancers of the nervous system.
  JOURNAL   JCI Insight. 2022 Oct 10;7(19):e158755. doi: 
            10.1172/jci.insight.158755.
  PUBMED    36040810
REFERENCE   2  (bases 1 to 9604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "JCI 
            Insight."; date: "2022-10-10"; volume: "7(19):e158755. doi"; pages: 
            " 10.1172/jci.insight.158755"
COMMENT     Sequence Label: pLX317-VRK1-K179E
FEATURES             Location/Qualifiers
     source          1..9604
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        83..1261
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          314..1252
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1209..1229
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1281..1305
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2500..2524)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2532..2573
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2624..3212
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2677..2697)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3095..3106)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3284..3517
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3595..3716
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3632..3651)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3686..3705
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3756..3891
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3911..3930)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3912..3930)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3940..3957)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3940..3956)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3949..3971)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4098..4553
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4185..4204)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4395..4416
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4579..4683
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4684..4752,4753..5544)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4902..4921)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5715..6303
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6204..6223
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6457..6474
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6591..6612
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6627..6632,6633..6650,6651..6657)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6665..6681
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6670..6692
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6689..6705
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6689..6705
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6724..6744
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6726..6744
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6772..6998
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6999..7179
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7226..7351
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7844..8077
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8262..8306
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8537..8747
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8559..8755
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8606..8741
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8787..9389
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8787..8806)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9283..9303
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9432..9549
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggtgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat ggaccttcta
       61 ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc
      121 cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg
      181 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg
      241 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc
      301 cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta
      361 tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga
      421 gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc
      481 tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca
      541 ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc
      601 tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac
      661 tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg
      721 ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc
      781 tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc
      841 aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc
      901 tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac
      961 acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg
     1021 ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg
     1081 gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag
     1141 gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg
     1201 gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg
     1261 aggctagcat cgattgatca acaagtttgt acaaaaaagc aggcttaatg cctcgtgtaa
     1321 aagcagctca agctggaaga cagagctctg caaagagaca tcttgcagaa caatttgcag
     1381 ttggagagat aataactgac atggcaaaaa aggaatggaa agtgggtctt ccaataggac
     1441 agggaggctt tggctgtata tatcttgctg atatgaattc ttcagagtca gttggcagtg
     1501 atgcaccttg tgttgtaaaa gtggaaccca gtgacaatgg acctcttttt actgaattaa
     1561 agttctacca acgagctgca aaaccagagc aaattcagaa atggattcgt acccgtaagc
     1621 tgaagtacct gggagtaccc aaatactggg gctctggtct acatgacaaa aatggaaaaa
     1681 gttacaggtt tatgataatg gatcgctttg ggagtgacct tcagaaaata tacgaggcta
     1741 acgcaaagag attttctcgg aaaactgtct tgcagctaag cttaagaatt ctggatattc
     1801 tggaatatat tcacgagcat gagtatgtgc atggagatat cgaggcctca aatcttcttc
     1861 tgaactacaa gaatcctgac caggtgtact tggttgacta cggactggca taccggtact
     1921 gcccagaagg agttcataaa gaatacaaag aagaccccaa aagatgtcac gatggcacta
     1981 ttgaattcac gagcatcgat gcacacaatg gcgtggcccc atcaagacgt ggtgatttgg
     2041 aaatacttgg ttattgcatg atccaatggc ttactggcca tcttccttgg gaggataatt
     2101 tgaaagatcc taaatatgtt agagattcca aaattagata cagagaaaat attgcaagtt
     2161 tgatggacaa atgttttcct gagaaaaaca aaccaggtga aattgccaaa tacatggaaa
     2221 cagtgaaatt actagactac actgaaaaac ctctttatga aaatttacgt gacattcttt
     2281 tgcaaggact aaaagctata ggttctaaag acgacggtaa gttggacctc agtgttgtgg
     2341 agaatggagg tttgaaagca aaaacaataa caaagaagcg aaagaaagaa attgaagaaa
     2401 gcaaggaacc tggtgttgaa gatacggaat ggtcaaacac acagacagag gaggccatac
     2461 agacccgttc aagaaccaga aagagagtcc agaagtaaga cccagctttc ttgtacaaag
     2521 tggttgatat cggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat
     2581 gaactagtac cggttaagtc gacaatcaac gcgttaagtc gacaatcaac ctctggatta
     2641 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     2701 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     2761 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     2821 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     2881 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     2941 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     3001 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     3061 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     3121 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     3181 gagtcggatc tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag
     3241 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     3301 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     3361 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     3421 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     3481 ctcagaccct tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat
     3541 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     3601 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     3661 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     3721 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     3781 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     3841 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt
     3901 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
     3961 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     4021 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     4081 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     4141 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     4201 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     4261 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     4321 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     4381 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     4441 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     4501 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
     4561 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     4621 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     4681 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     4741 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     4801 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     4861 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     4921 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     4981 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     5041 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     5101 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     5161 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     5221 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     5281 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     5341 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     5401 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     5461 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     5521 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     5581 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     5641 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     5701 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     5761 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     5821 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     5881 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     5941 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     6001 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     6061 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     6121 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6181 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     6241 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6301 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6361 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     6421 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     6481 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     6541 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     6601 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     6661 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     6721 cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt
     6781 atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag
     6841 gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat
     6901 taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca
     6961 gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga
     7021 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     7081 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     7141 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     7201 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     7261 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     7321 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     7381 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     7441 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     7501 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     7561 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     7621 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     7681 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     7741 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     7801 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     7861 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     7921 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     7981 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     8041 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     8101 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     8161 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     8221 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     8281 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     8341 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     8401 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     8461 tcccaacccc gaggggaccc atgcattgca tctcaattag tcagcaacca ggtgtggaaa
     8521 gtccccaggc tccccagcag gcagaagtat gcaaagcatg cgtctcaatt agtcagcaac
     8581 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     8641 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     8701 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     8761 ttctagaggt accaccggag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca
     8821 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     8881 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     8941 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     9001 ccgtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     9061 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     9121 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     9181 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     9241 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     9301 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     9361 gcatgacccg caagcccggt gccagttaac gaattctaga tcttgagaca aatggcagta
     9421 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     9481 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     9541 caaaattttc gggtttatta cagggacagc agagatccac tttggcgccg gctcgaggcc
     9601 tgca
//
