LOCUS       sequence-394141-        3402 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express full length human PLD3 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Yuan M, Peng L, Huang D, Gavin A, Luan F, Tran J, Feng Z, Zhu X, 
            Matteson J, Wilson IA, Nemazee D
  TITLE     Structural and mechanistic insights into disease-associated 
            endolysosomal exonucleases PLD3 and PLD4.
  JOURNAL   Structure. 2024 Jun 6;32(6):766-779.e7. doi: 
            10.1016/j.str.2024.02.019. Epub 2024 Mar 26.
  PUBMED    38537643
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.str.2024.02.019"; journalName: "Structure"; date: 
            "2024-06-6- 6"; volume: "32"; issue: "6"; pages: "766-779.e7"
COMMENT     Sequence Label: SuExp-hPLD3
FEATURES             Location/Qualifiers
     source          1..3402
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    88..222
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(112..131)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     166..185
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(265..853)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(345..364)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(878..1252)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(1253..1316)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        1355..1734
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1735..1938
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1889..1909
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1935..1959
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ggactgggac tccccttaca gccatgacct tgacacctca gctgacagcg tgggcaacgc
       61 ctgccgcctg ctctaaatct agaagctgat ccagacatga taagatacat tgatgagttt
      121 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
      181 attgctttat ttgtaaccat tataagctgc aataaacaag ttggatatcc tcgagactag
      241 tgggcccgtt taaacacatg tgtttttcca taggctccgc ccccctgacg agcatcacaa
      301 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
      361 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
      421 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
      481 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
      541 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
      601 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
      661 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
      721 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
      781 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
      841 aaaaggatct caagaagatc ctttgatctt ttctacgtca gtcctgctcc tcggccacga
      901 agtgcacgca gttgccggcc gggtcgcgca gggcgaactc ccgcccccac ggctgctcgc
      961 cgatctcggt catggccggc ccggaggcgt cccggaagtt cgtggacacg acctccgacc
     1021 actcggcgta cagctcgtcc aggccgcgca cccacaccca ggccagggtg ttgtccggca
     1081 ccacctggtc ctggaccgcg ctgatgaaca gggtcacgtc gtcccggacc acaccggcga
     1141 agtcgtcctc cacgaagtcc cgggagaacc cgagccggtc ggtccagaac tcgaccgctc
     1201 cggcgacgtc gcgcgcggtg agcaccggaa cggcactggt caacttggcc atactcttcc
     1261 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacataacgc
     1321 gtcgcgaggc catatgggtt aacccatggc cgttgacatt gattattgac tagttattaa
     1381 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
     1441 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     1501 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     1561 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
     1621 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     1681 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
     1741 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
     1801 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
     1861 aaatgtcgta ataaccccgc cccgttgacg caaatgggcg gtaggcgtgt acggtgggag
     1921 gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg tcgagccgag
     1981 tggttgtgcc tccatagaag ccaccatgaa gcctaaactg atgtaccagg agctgaaggt
     2041 gcctgcagag gagcccgcca atgagctgcc catgaatgag attgaggcgt ggaaggctgc
     2101 ggaaaagaaa gcccgctggg tcctgctggt cctcattctg gcggttgtgg gcttcggagc
     2161 cctgatgact cagctgtttc tatgggaata cggcgacttg catctctttg ggcccaacca
     2221 gcgcccagcc ccctgctatg acccttgcga agcagtgctg gtggaaagca ttcctgaggg
     2281 cctggacttc cccaatgcct ccacggggaa cccttccacc agccaggcct ggctgggcct
     2341 gctcgccggt gcgcacagca gcctggacat cgcctccttc tactggaccc tcaccaacaa
     2401 tgacacccac acgcaggagc cctctgccca gcagggtgag gaggtcctcc ggcagctgca
     2461 gaccctggca ccaaagggcg tgaacgtccg catcgctgtg agcaagccca gcgggcccca
     2521 gccacaggcg gacctgcagg ctctgctgca gagcggtgcc caggtccgca tggtggacat
     2581 gcagaagctg acccatggcg tcctgcatac caagttctgg gtggtggacc agacccactt
     2641 ctacctgggc agtgccaaca tggactggcg ttcactgacc caggtcaagg agctgggcgt
     2701 ggtcatgtac aactgcagct gcctggctcg agacctgacc aagatctttg aggcctactg
     2761 gttcctgggc caggcaggca gctccatccc atcaacttgg ccccggttct atgacacccg
     2821 ctacaaccaa gagacaccaa tggagatctg cctcaatgga acccctgctc tggcctacct
     2881 ggcgagtgcg cccccacccc tgtgtccaag tggccgcact ccagacctga aggctctact
     2941 caacgtggtg gacaatgccc ggagtttcat ctacgtcgct gtcatgaact acctgcccac
     3001 tctggagttc tcccaccctc acaggttctg gcctgccatt gacgatgggc tgcggcgggc
     3061 cacctacgag cgtggcgtca aggtgcgcct gctcatcagc tgctggggac actcggagcc
     3121 atccatgcgg gccttcctgc tctctctggc tgccctgcgt gacaaccata cccactctga
     3181 catccaggtg aaactctttg tggtccccgc ggatgaggcc caggctcgaa tcccatatgc
     3241 ccgtgtcaac cacaacaagt acatggtgac tgaacgcgcc acctacatcg gaacctccaa
     3301 ctggtctggc aactacttca cggagacggc gggcacctcg ctgctggtga cgcagaatgg
     3361 gaggggcggc ctgcggagcc agctggaggc cattttcctg ag
//
