LOCUS       sequence-394145-        7972 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline-inducible BAF expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7972)
  AUTHORS   So J, Mabe NW, Englinger B, Chow KH, Moyer SM, Yerrum S, Trissal MC,
            Marques JG, Kwon JJ, Shim B, Pal S, Panditharatna E, Quinn T, 
            Schaefer DA, Jeong D, Mayhew DL, Hwang J, Beroukhim R, Ligon KL, 
            Stegmaier K, Filbin MG, Hahn WC
  TITLE     VRK1 as a synthetic lethal target in VRK2 promoter-methylated 
            cancers of the nervous system.
  JOURNAL   JCI Insight. 2022 Oct 10;7(19):e158755. doi: 
            10.1172/jci.insight.158755.
  PUBMED    36040810
REFERENCE   2  (bases 1 to 7972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "JCI 
            Insight."; date: "2022-10-10"; volume: "7(19):e158755. doi"; pages: 
            " 10.1172/jci.insight.158755"
COMMENT     Sequence Label: BAF-WT-tet-puro
FEATURES             Location/Qualifiers
     source          1..7972
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    46..64
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    82..100
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    117..135
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    153..171
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    189..207
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    224..242
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    260..278
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    340..457
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(670..714)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    899..1132
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1625..1750
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1797..1977
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1978..2204)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(2232..2252)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2232..2250)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2271..2287)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2271..2287)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2284..2306)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2295..2311
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2319..2325,2326..2343,2344..2349))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2364..2385
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2502..2519)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2673..3261)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2753..2772)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3432..4223,4224..4292))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4055..4074
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4293..4364)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4364..4499
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4539..4660
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4550..4569)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4604..4623
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4732..4965
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             5170..5181
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5579..5599
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(5639..5647,5648..5683,5684..5686,5687..5722,
                     5723..5725,5726..5761,5762..5767,5768..6385))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(6386..6439)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(6440..7036)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6520..6540)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     7017..7036
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(7046..7556)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7109..7127)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             complement(7566..7583)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    7601..7625
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7899..7923)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(join(7944..7972,1..286))
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(7947..7971)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 tgcttatata ggcctcccac cgtacacgcc tacctcgaca tacgttctct atcactgata
       61 gggagtaaac tcgacatacg ttctctatca ctgataggga taaactcgac atacgttctc
      121 tatcactgat agggagtaaa ctcgacatac gttctctatc actgataggg agtaaactcg
      181 acatacgttc tctatcactg atagggagta aactcgacat cgttctctat cactgatagg
      241 gagtaaactc gacatacgtt ctctatcact gatagggagt aaactcgaca tatcgattcg
      301 cggccaaagt ggatctctgc tgtccctgta ataaacccga aaattttgaa tttttgtaat
      361 ttgtttttgt aattctttag tttgtatgtc tgttgctatt atgtctacta ttctttcccc
      421 tgcactgtac cccccaatcc ccccttttct tttaaaattg tggatgaata ctgccatttg
      481 tctcgaggtc gagaattgtc ccctcggggt tgggaggtgg gtctgaaacg ataatggtga
      541 atatccctgc ctaactctat tcactataga aagtacagca aaaactattc ttaaacctac
      601 caagcctcct actatcatta tgaataattt tatataccac agccaatttg ttatgttaaa
      661 ccaattccac aaacttgccc atttatctaa ttccaataat tcttgttcat tcttttcttg
      721 ctggttttgc gattcttcaa ttaaggagtg tattaagctt gtgtaattgt taatttctct
      781 gtcccactcc atccaggtcg tgtgattcca aatctgttcc agagatttat tactccaact
      841 agcattccaa ggcacagcag tggtgcaaat gagttttcca gagcaacccc aaatccccag
      901 gagctgttga tcctttaggt atctttccac agccaggatt cttgcctgga gctgcttgat
      961 gccccagact gtgagttgca acagatgctg ttgcgcctca atagccctca gcaaattgtt
     1021 ctgctgctgc actataccag acaataattg tctggcctgt accgtcagcg tcattgacgc
     1081 tgcgcccata gtgcttcctg ctgctcccaa gaacccaagg aacaaagctc ctattcccac
     1141 tgctcttttt tctctctgca ccactcttct ctttgccttg gtgggtgcta ctcctaatgg
     1201 ttcaattttt actactttat atttatataa ttcacttctc caattgtccc tcatatctcc
     1261 tcctccaggt ctgaagatca gcggccgctt gctgtgcggt ggtcttactt ttgttttgct
     1321 cttcctctat cttgtctaaa gcttccttgg tgtcttttat ctctatcctt tgatgcacac
     1381 aatagagggt tgctactgta ttatataatg atctaagttc ttctgatcct gtctgaaggg
     1441 atggttgtag ctgtcccagt atttgtctac agccttctga tgtttctaac aggccaggat
     1501 taactgcgaa tcgttctagc tccctgcttg cccatactat atgttttaat ttatattttt
     1561 tctttccccc tggccttaac cgaatttttt cccatcgcga tctaattctc ccccgcttaa
     1621 tactgacgct ctcgcaccca tctctctcct tctagcctcc gctagtcaaa atttttggcg
     1681 tactcaccag tcgccgcccc tcgcctcttg ccgtgcgcgc ttcagcaagc cgagtcctgc
     1741 gtcgagagag ctcctctggt ttccctttcg ctttcaagtc cctgttcggg cgccactgct
     1801 agagattttc cacactgact aaaagggtct gagggatctc tagttaccag agtcacacaa
     1861 cagacgggca cacactactt gaagcactca aggcaagctt tattgaggct taagcagtgg
     1921 gttccctagt tagccagaga gctcccaggc tcagatctgg tctaaccaga gagacccgtt
     1981 tatgtatcga gctaggcact taaatacaat atctctgcaa tgcggcaatt cagtggttcg
     2041 tccaatccat gtcagacccg tctgttgcct tcctaataag gcacgatcgt accaccttac
     2101 ttccaccaat cggcatgcac ggtgcttttt ctctccttgt aaggcatgtt gctaactcat
     2161 cgttaccatg ttgcaagact acaagagtat tgcataagac tacattaagc ttgcagctcc
     2221 agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg
     2281 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     2341 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     2401 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     2461 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     2521 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     2581 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     2641 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     2701 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     2761 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     2821 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     2881 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     2941 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     3001 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     3061 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     3121 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     3181 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     3241 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     3301 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     3361 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     3421 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     3481 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     3541 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     3601 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     3661 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     3721 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     3781 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     3841 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     3901 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     3961 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     4021 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     4081 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     4141 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     4201 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     4261 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     4321 atttatcagg gttattgtct catgagcgga tacatatttg aaaggcctcc aaaaaagcct
     4381 cctcactact tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa
     4441 aaaattagtc agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg
     4501 ggcggagtta ggggcgggat agctagagcc agacatgata agatacattg atgagtttgg
     4561 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     4621 tgctttattt gtaaccatta taagctgcaa taaacaagtt cctctcactc tctgatattc
     4681 atttctttgc aagttataaa tactgaataa taagatgaca tgaactacta ctgctagaga
     4741 ttttccacac tgactaaaag ggtctgaggg atctctagtt accagagtca cacaacagac
     4801 gggcacacac tacttgaagc actcaaggca agctttattg aggcttaagc agtgggttcc
     4861 ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac ccagtacaag
     4921 caaaaagcag atcttgtctt cgttgggagt gaattagccc ttccagtccc cccttttctt
     4981 ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa ggtacctcta
     5041 gtccggacgc cgaggcgaaa caggcgggga ggcggcccaa agggagatcc gactcgtctg
     5101 agggcgaagg cgaagacgcg gaagaggccg cagagccggc agcaggccgc gggaaggaag
     5161 gtccgctgga ttgagggccg aagggacgta gcagaaggac gtcccgcgca gaatccaggt
     5221 ggcaacacag gcgagcagcc atggaaagga cgtcagcttc cccgacaaca ccacggaatt
     5281 gtcagtgccc aacagccgag cccctgtcca gcagcgggca aggcaggcgg cgatgagttc
     5341 cgccgtggca atagggaggg ggaaagcgaa agtcccggaa aggagctgac aggtggtggc
     5401 aatgccccaa ccagtggggg ttgcgtcagc aaacacagtg cacaccacgc cacgttgcct
     5461 gacaacgggc cacaactcct cataaagaga cagcaaccag gatttataca aggaggagaa
     5521 aatgaaagcc atacgggaag caatagcatg atacaaaggc attaaagcag cgtatccaca
     5581 tagcgtaaaa ggagcaacat agttaagaat acgatatctt ggatcgatcc ttacttagtt
     5641 acccggggag catgtcaagg tcaaaatcgt caagagcgtc agcaggcagc atatcaaggt
     5701 caaagtcgtc aagggcatcg gctgggagca tgtctaagtc aaaatcgtca agggcgtcgg
     5761 tcggcccgcc gctttcgcac tttagctgtt tctccaggcc acatatgatt agttccaggc
     5821 cgaaaaggaa ggcaggttcg gctccctgcc ggtcgaacag ctcaattgct tgtctcagaa
     5881 gtgggggcat agaatcggtg gtaggtgtct ctctttcctc ttttgctact tgatgctcct
     5941 gttcctccaa tacgcagccc agtgtaaagt ggcccacggc ggacagagcg tacagtgcgt
     6001 tctccaggga gaagccttgc tgacacagga acgcgagctg attttccagg gtttcgtact
     6061 gtttctctgt tgggcgggtg ccgagatgca ctttagcccc gtcgcgatgt gagaggagag
     6121 cacagcggta tgacttggcg ttgttccgca gaaagtcttg ccatgactcg ccttccaggg
     6181 ggcagaagtg ggtatgatgc ctgtccagca tctcgattgg cagggcatcg agcagggccc
     6241 gcttgttctt cacgtgccag tacagggtag gctgctcaac tcccagcttt tgagcgagtt
     6301 tccttgtcgt caggccttcg ataccgacac cattgagtaa ttccagagct ccgtttatga
     6361 ctttgctctt gtccagtcta gacattggac cagggttttc ttcaacatca ccacaagtga
     6421 ggagagaacc tctaccttcg gcaccgggct tgcgggtcat gcaccaggtg cgcggtcctt
     6481 cgggcacctc gacgtcggcg gtgacggtga agccgagccg ctcgtagaag gggaggttgc
     6541 ggggcgcgga ggtctccagg aaggcgggca ccccggcgcg ctcggccgcc tccactccgg
     6601 ggagcacgac ggcgctgccc agacccttgc cctggtggtc gggcgagacg ccgacggtgg
     6661 ccaggaacca cgcgggctcc ttgggccggt gcggcgccag gaggccttcc atctgttgct
     6721 gcgcggccag ccgggaaccg ctcaactcgg ccatgcgcgg gccgatctcg gcgaacaccg
     6781 cccccgcttc gacgctctcc ggcgtggtcc agaccgccac cgcggcgccg tcgtccgcga
     6841 cccacacctt gccgatgtcg agcccgacgc gcgtgaggaa gagttcttgc agctcggtga
     6901 cccgctcgat gtggcggtcc ggatcgacgg tgtggcgcgt ggcggggtag tcggcgaacg
     6961 cggcggcgag ggtgcgtacg gccctgggga cgtcgtcgcg ggtggcgagg cgcaccgtgg
     7021 gcttgtactc ggtcatggtg aattgctggg gagagaggtc ggtgattcgg tcaacgaggg
     7081 agccgactgc cgacgtgcgc tccggaggct tgcagaatgc ggaacaccgc gcgggcagga
     7141 acagggccca cactaccgcc ccacaccccg cctcccgcac cgccccttcc cggccgctgc
     7201 tctcggcgcg ccctgctgag cagccgctat tggccacagc ccatcgcggt cggcgcgctg
     7261 ccattgctcc ctggcgctgt ccgtctgcga gggtactagt gagacgtgcg gcttccgttt
     7321 gtcacgtccg gcacgccgcg aaccgcaagg aaccttcccg acttaggggc ggagcaggaa
     7381 gcgtcgccgg ggggcccaca agggtagcgg cgaagatccg ggtgacgctg cgaacggacg
     7441 tgaagaatgt gcgagaccca gggtcggcgc cgctgcgttt cccggaacca cgcccagagc
     7501 agccgcgtcc ctgcgcaaac ccagggctgc cttggaaaag gcgcaacccc aaccccggat
     7561 ccttagtggt ggtggtggtg gtggaccggt tcattactaa accactttgt acaagaaagc
     7621 tgggtgcaag aaggcgtcgc accactctcg aaggcatccg aagcagtccc gggactgctt
     7681 ggcgttggcg ccacaagtgt ctttcagcca ttcccggaag aggtcttcat ctttctttag
     7741 caccagaaac tggccaagga caacataggc cttgtcaaaa cccctttcct ccagcttctt
     7801 gcccaggact tcaccaatcc cagccaggct ccccactggc ttctccccca tgggctctgc
     7861 cacgaagtca cgatgtttct gggaggttgt catggtgaag cctgcttttt tgtacaaact
     7921 tgttgatgct agccaattct ccaggcgatc tgacggttca ctaaacgagc tc
//
