LOCUS       sequence-39415-e         703 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Weinman EJ, Steplock D, Tate K, Hall RA, Spurney RF, Shenolikar S
  TITLE     Structure-function of recombinant Na/H exchanger regulatory factor 
            (NHE-RF).
  JOURNAL   J Clin Invest. 1998 May 15;101(10):2199-206.
  PUBMED    9593775
REFERENCE   2  (bases 1 to 703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest."; date: "1998-05-15"; volume: "101(10)"; pages: "2199-206"
COMMENT     Sequence Label: pET30 NHERF1
FEATURES             Location/Qualifiers
     source          1..703
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    80..104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             119..141
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             152..169
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             179..196
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             203..247
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             263..277
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 tgagccggcc acgaatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat
       61 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
      121 tgtttaactt taagaaggag atatacatat gcaccatcat catcatcatt cttctggtct
      181 ggtgccacgc ggttctggta tgaaagaaac cgctgctgct aaattcgaac gccagcacat
      241 ggacagccca gatctgggta ccgacgacga cgacaaggcc atggctgatc gcgccgctga
      301 gccgtcgcgg ggcgagatga gcgcggacgc ggcggccggg gcgcccctgc cccggctctg
      361 ctgcctggag aaggggccga acggctacgg cttccacctg catggggaga agggcaaggt
      421 gggccagtac atccggctgg tggagcccgg ctctccggcc gagaaggcgg ggctgctggc
      481 cggggaccgg ctggtggagg tgaacggcga gaacgtggag aaggagaccc accagcaggt
      541 ggtgagccgc atccgcgccg cgctcaacgc cgtgcgcctg ctggtggtcg accccgacac
      601 ggacgagcag ttcaggaagc tcggagtcca gatccggggg gagctgctac gcgcccaggc
      661 ggggccggag caggccgggc cgccggccgc ccccggcgag cag
//
