LOCUS       sequence-394150-        9604 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses VRK1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9604)
  AUTHORS   So J, Mabe NW, Englinger B, Chow KH, Moyer SM, Yerrum S, Trissal MC,
            Marques JG, Kwon JJ, Shim B, Pal S, Panditharatna E, Quinn T, 
            Schaefer DA, Jeong D, Mayhew DL, Hwang J, Beroukhim R, Ligon KL, 
            Stegmaier K, Filbin MG, Hahn WC
  TITLE     VRK1 as a synthetic lethal target in VRK2 promoter-methylated 
            cancers of the nervous system.
  JOURNAL   JCI Insight. 2022 Oct 10;7(19):e158755. doi: 
            10.1172/jci.insight.158755.
  PUBMED    36040810
REFERENCE   2  (bases 1 to 9604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "JCI 
            Insight."; date: "2022-10-10"; volume: "7(19):e158755. doi"; pages: 
            " 10.1172/jci.insight.158755"
COMMENT     Sequence Label: pLX317-VRK1
FEATURES             Location/Qualifiers
     source          1..9604
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        76..1254
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          307..1245
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1202..1222
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1274..1298
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2493..2517)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2525..2566
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2617..3205
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2670..2690)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3088..3099)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3277..3510
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3588..3709
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3625..3644)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3679..3698
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3749..3884
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3904..3923)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3905..3923)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3933..3950)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3933..3949)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3942..3964)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4091..4546
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4178..4197)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4388..4409
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4572..4676
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4677..4745,4746..5537)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4895..4914)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5708..6296
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6197..6216
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6450..6467
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6584..6605
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6620..6625,6626..6643,6644..6650)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6658..6674
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6663..6685
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6682..6698
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6682..6698
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6717..6737
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6719..6737
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6765..6991
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6992..7172
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7219..7344
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7837..8070
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8255..8299
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8530..8740
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8552..8748
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8599..8734
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8780..9382
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8780..8799)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9276..9296
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9425..9542
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 agatggataa agttttaaac agagaggaat ctttgcagct aatggacctt ctaggtcttg
       61 aaaggagtgg gaattggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
      121 cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg
      181 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
      241 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
      301 acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct
      361 tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg
      421 gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct
      481 tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc
      541 gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg
      601 acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt
      661 tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg
      721 aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg
      781 cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg
      841 gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg
      901 agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg
      961 aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg
     1021 tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag
     1081 gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct
     1141 tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt
     1201 ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgaggctag
     1261 catcgattga tcaacaagtt tgtacaaaaa agcaggctta atgcctcgtg taaaagcagc
     1321 tcaagctgga agacagagct ctgcaaagag acatcttgca gaacaatttg cagttggaga
     1381 gataataact gacatggcaa aaaaggaatg gaaagtgggt cttccaatag gacagggagg
     1441 ctttggctgt atatatcttg ctgatatgaa ttcttcagag tcagttggca gtgatgcacc
     1501 ttgtgttgta aaagtggaac ccagtgacaa tggacctctt tttactgaat taaagttcta
     1561 ccaacgagct gcaaaaccag agcaaattca gaaatggatt cgtacccgta agctgaagta
     1621 cctgggagta cccaaatact ggggctctgg tctacatgac aaaaatggaa aaagttacag
     1681 gtttatgata atggatcgct ttgggagtga ccttcagaaa atatacgagg ctaacgcaaa
     1741 gagattttct cggaaaactg tcttgcagct aagcttaaga attctggata ttctggaata
     1801 tattcacgag catgagtatg tgcatggaga tatcaaggcc tcaaatcttc ttctgaacta
     1861 caagaatcct gaccaggtgt acttggttga ctacggactg gcataccggt actgcccaga
     1921 aggagttcat aaagaataca aagaagaccc caaaagatgt cacgatggca ctattgaatt
     1981 cacgagcatc gatgcacaca atggcgtggc cccatcaaga cgtggtgatt tggaaatact
     2041 tggttattgc atgatccaat ggcttactgg ccatcttcct tgggaggata atttgaaaga
     2101 tcctaaatat gttagagatt ccaaaattag atacagagaa aatattgcaa gtttgatgga
     2161 caaatgtttt cctgagaaaa acaaaccagg tgaaattgcc aaatacatgg aaacagtgaa
     2221 attactagac tacactgaaa aacctcttta tgaaaattta cgtgacattc ttttgcaagg
     2281 actaaaagct ataggttcta aagacgacgg taagttggac ctcagtgttg tggagaatgg
     2341 aggtttgaaa gcaaaaacaa taacaaagaa gcgaaagaaa gaaattgaag aaagcaagga
     2401 acctggtgtt gaagatacgg aatggtcaaa cacacagaca gaggaggcca tacagacccg
     2461 ttcaagaacc agaaagagag tccagaagta agacccagct ttcttgtaca aagtggttga
     2521 tatcggtaag cctatcccta accctctcct cggtctcgat tctacgtagt aatgaactag
     2581 taccggttaa gtcgacaatc aacgcgttaa gtcgacaatc aacctctgga ttacaaaatt
     2641 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     2701 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     2761 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     2821 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     2881 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     2941 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     3001 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     3061 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     3121 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     3181 atctcccttt gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg
     3241 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     3301 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     3361 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     3421 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     3481 ccttttagtc agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta
     3541 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     3601 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     3661 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     3721 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     3781 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     3841 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     3901 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     3961 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     4021 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     4081 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     4141 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     4201 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     4261 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     4321 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     4381 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     4441 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     4501 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     4561 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     4621 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     4681 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     4741 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     4801 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     4861 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     4921 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     4981 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     5041 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     5101 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     5161 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     5221 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     5281 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     5341 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     5401 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     5461 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     5521 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     5581 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     5641 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     5701 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     5761 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     5821 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     5881 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     5941 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     6001 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     6061 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     6121 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     6181 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     6241 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     6301 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     6361 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     6421 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     6481 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     6541 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     6601 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     6661 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     6721 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat
     6781 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
     6841 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
     6901 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat
     6961 tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc
     7021 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     7081 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     7141 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     7201 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     7261 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     7321 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     7381 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     7441 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     7501 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     7561 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     7621 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     7681 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     7741 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     7801 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     7861 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     7921 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     7981 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     8041 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     8101 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     8161 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     8221 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     8281 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     8341 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     8401 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     8461 cccgagggga cccatgcatt gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     8521 ggctccccag caggcagaag tatgcaaagc atgcgtctca attagtcagc aaccatagtc
     8581 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     8641 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     8701 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctttctaga
     8761 ggtaccaccg gagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     8821 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     8881 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     8941 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgccgtggc
     9001 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     9061 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     9121 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     9181 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     9241 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     9301 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     9361 ccgcaagccc ggtgccagtt aacgaattct agatcttgag acaaatggca gtattcatcc
     9421 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     9481 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     9541 ttcgggttta ttacagggac agcagagatc cactttggcg ccggctcgag gcctgcaggt
     9601 gcaa
//
