LOCUS       sequence-394153-        9940 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses VRK2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9940)
  AUTHORS   So J, Mabe NW, Englinger B, Chow KH, Moyer SM, Yerrum S, Trissal MC,
            Marques JG, Kwon JJ, Shim B, Pal S, Panditharatna E, Quinn T, 
            Schaefer DA, Jeong D, Mayhew DL, Hwang J, Beroukhim R, Ligon KL, 
            Stegmaier K, Filbin MG, Hahn WC
  TITLE     VRK1 as a synthetic lethal target in VRK2 promoter-methylated 
            cancers of the nervous system.
  JOURNAL   JCI Insight. 2022 Oct 10;7(19):e158755. doi: 
            10.1172/jci.insight.158755.
  PUBMED    36040810
REFERENCE   2  (bases 1 to 9940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "JCI 
            Insight."; date: "2022-10-10"; volume: "7(19):e158755. doi"; pages: 
            " 10.1172/jci.insight.158755"
COMMENT     Sequence Label: pLX317-VRK2
FEATURES             Location/Qualifiers
     source          1..9940
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        83..1261
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          314..1252
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1209..1229
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1281..1305
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             2445..2456
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    complement(2836..2860)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2868..2909
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2960..3548
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3013..3033)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3431..3442)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3620..3853
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3931..4052
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3968..3987)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4022..4041
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4092..4227
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4247..4266)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4248..4266)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4276..4293)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4276..4292)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4285..4307)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4434..4889
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4521..4540)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4731..4752
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4915..5019
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5020..5088,5089..5880)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5238..5257)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6051..6639
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6540..6559
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6793..6810
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6927..6948
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6963..6968,6969..6986,6987..6993)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7001..7017
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7006..7028
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7025..7041
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7025..7041
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7060..7080
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7062..7080
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7108..7334
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7335..7515
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7562..7687
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8180..8413
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8598..8642
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8873..9083
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8895..9091
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8942..9077
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9123..9725
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9123..9142)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9619..9639
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9768..9885
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggtgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat ggaccttcta
       61 ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc
      121 cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg
      181 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg
      241 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc
      301 cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta
      361 tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga
      421 gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc
      481 tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca
      541 ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc
      601 tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac
      661 tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg
      721 ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc
      781 tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc
      841 aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc
      901 tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac
      961 acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg
     1021 ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg
     1081 gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag
     1141 gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg
     1201 gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg
     1261 aggctagcat cgattgatca acaagtttgt acaaaaaagc aggcaccatg ccaccaaaaa
     1321 gaaatgaaaa atacaaactt cctattccat ttccagaagg caaggttctg gatgatatgg
     1381 aaggcaatca gtgggtactg ggcaagaaga ttggctctgg aggatttgga ttgatatatt
     1441 tagctttccc cacaaataaa ccagagaaag atgcaagaca tgtagtaaaa gtggaatatc
     1501 aagaaaatgg cccgttattt tcagaactta aattttatca gagagttgca aaaaaagact
     1561 gtatcaaaaa gtggatagaa cgcaaacaac ttgattattt aggaattcct ctgttttatg
     1621 gatctggtct gactgaattc aagggaagaa gttacagatt tatggtaatg gaaagactag
     1681 gaatagattt acagaagatc tcaggccaga atggtacctt taaaaagtca actgtcctgc
     1741 aattaggtat ccgaatgttg gatgtactgg aatatataca tgaaaatgaa tatgttcatg
     1801 gtgatataaa agcagcaaat ctacttttgg gttacaaaaa tccagaccag gtttatcttg
     1861 cagattatgg actttcctac agatattgtc ccaatgggaa ccacaaacag tatcaggaaa
     1921 atcctagaaa aggccataat gggacaatag agtttaccag cttggatgcc cacaagggag
     1981 tagccttgtc cagacgaagt gacgttgaga tcctcggcta ctgcatgctg cggtggttgt
     2041 gtgggaaact tccctgggaa cagaacctga aggaccctgt ggctgtgcag actgctaaaa
     2101 caaatctgtt ggacgagctc ccccagtcag tgcttaaatg ggctccttct ggaagcagtt
     2161 gctgtgaaat agcccaattt ttggtatgtg ctcatagttt agcatatgat gaaaagccaa
     2221 actatcaagc cctcaagaaa attttgaacc ctcatggaat acctttagga ccactggact
     2281 tttccacaaa aggacagagt ataaatgtcc atactccaaa cagtcaaaaa gttgattcac
     2341 aaaaggctgc aacaaagcaa gtcaacaagg cacacaatag gttaatcgaa aaaaaagtcc
     2401 acagtgagag aagcgctgag tcctgtgcaa catggaaagt gcagaaagag gagaaactga
     2461 ttggattgat gaacaatgaa gcagctcagg aaagcacaag gagaagacag aaatatcaag
     2521 agtctcaaga acctttgaat gaagtaaaca gtttcccaca aaaaatcagc tatacacaat
     2581 tcccaaactc attttatgag cctcatcaag attttaccag tccagatata ttcaagaagt
     2641 caagatctcc atcttggtat aaatacactt ccacagtcag cacggggatc acagacttag
     2701 aaagttcaac tggactttgg cctacaattt cccagtttac tcttagtgaa gagacaaacg
     2761 cagatgttta ttattatcgc atcatcatac ctgtcctttt gatgttagta tttcttgctt
     2821 tattttttct ctgagaccca gctttcttgt acaaagtggt tgatatcggt aagcctatcc
     2881 ctaaccctct cctcggtctc gattctacgt agtaatgaac tagtaccggt taagtcgaca
     2941 atcaacgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     3001 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     3061 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     3121 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     3181 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     3241 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     3301 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     3361 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     3421 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     3481 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     3541 ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     3601 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     3661 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     3721 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     3781 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     3841 aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt
     3901 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     3961 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     4021 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     4081 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     4141 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     4201 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     4261 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     4321 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     4381 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     4441 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     4501 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     4561 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     4621 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     4681 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     4741 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     4801 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     4861 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     4921 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     4981 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     5041 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     5101 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     5161 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     5221 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     5281 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     5341 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     5401 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     5461 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     5521 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     5581 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     5641 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     5701 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     5761 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     5821 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     5881 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     5941 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     6001 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6061 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6121 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6181 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     6241 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6301 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6361 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6421 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     6481 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     6541 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     6601 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     6661 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     6721 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     6781 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     6841 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     6901 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     6961 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     7021 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
     7081 gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca
     7141 acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc
     7201 gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga
     7261 catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag
     7321 ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct
     7381 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     7441 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     7501 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     7561 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     7621 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     7681 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     7741 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     7801 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     7861 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     7921 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     7981 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     8041 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     8101 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     8161 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     8221 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     8281 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     8341 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     8401 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     8461 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     8521 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     8581 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     8641 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     8701 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     8761 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccatgc
     8821 attgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     8881 aagtatgcaa agcatgcgtc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     8941 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     9001 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     9061 aggctttttt ggaggcctag gcttttgcaa aaagctttct agaggtacca ccggagctta
     9121 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     9181 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     9241 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     9301 acatcggcaa ggtgtgggtc gcggacgacg gcgccgccgt ggcggtctgg accacgccgg
     9361 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     9421 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     9481 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     9541 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     9601 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     9661 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcca
     9721 gttaacgaat tctagatctt gagacaaatg gcagtattca tccacaattt taaaagaaaa
     9781 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     9841 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
     9901 gacagcagag atccactttg gcgccggctc gaggcctgca
//
