LOCUS       sequence-394158-        9157 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses VRK1 C-term. Tagged with dTAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9157)
  AUTHORS   So J, Mabe NW, Englinger B, Chow KH, Moyer SM, Yerrum S, Trissal MC,
            Marques JG, Kwon JJ, Shim B, Pal S, Panditharatna E, Quinn T, 
            Schaefer DA, Jeong D, Mayhew DL, Hwang J, Beroukhim R, Ligon KL, 
            Stegmaier K, Filbin MG, Hahn WC
  TITLE     VRK1 as a synthetic lethal target in VRK2 promoter-methylated 
            cancers of the nervous system.
  JOURNAL   JCI Insight. 2022 Oct 10;7(19):e158755. doi: 
            10.1172/jci.insight.158755.
  PUBMED    36040810
REFERENCE   2  (bases 1 to 9157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "JCI 
            Insight."; date: "2022-10-10"; volume: "7(19):e158755. doi"; pages: 
            " 10.1172/jci.insight.158755"
COMMENT     Sequence Label: VRK1-dTag-puro
FEATURES             Location/Qualifiers
     source          1..9157
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    221..342
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(258..277)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     312..331
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      382..517
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(537..556)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(538..556)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(566..583)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(566..582)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(575..597)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      724..1179
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(811..830)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1021..1042
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1205..1309
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1310..1378,1379..2170)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1528..1547)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2341..2929
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2830..2849
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3083..3100
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3217..3238
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3253..3258,3259..3276,3277..3283)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3291..3307
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3296..3318
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3315..3331
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3315..3331
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3350..3370
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3352..3370
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3398..3624
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3625..3805
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3852..3977
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4470..4703
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4888..4932
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        5163..5373
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        5185..5381
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5232..5367
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5413..6015
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(5413..5432)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5909..5929
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6058..6175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6233..6733
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6652..6670
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    6754..6778
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7970..7994)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8002..8322
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             8329..8355
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             8329..8355
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             8359..8385
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    8407..8995
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8460..8480)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8878..8889)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             join(9067..9157,1..143)
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
       61 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
      121 gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta
      181 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
      241 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
      301 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
      361 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
      421 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
      481 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
      541 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
      601 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
      661 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
      721 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
      781 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      841 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      901 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      961 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     1021 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     1081 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     1141 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     1201 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     1261 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     1321 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     1381 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     1441 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     1501 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     1561 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     1621 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     1681 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     1741 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     1801 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     1861 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     1921 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     1981 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     2041 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     2101 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     2161 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     2221 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     2281 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     2341 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     2401 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     2461 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     2521 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     2581 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     2641 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     2701 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     2761 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     2821 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     2881 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     2941 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     3001 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     3061 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     3121 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     3181 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     3241 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     3301 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     3361 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     3421 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     3481 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     3541 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     3601 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     3661 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     3721 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     3781 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     3841 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     3901 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     3961 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     4021 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     4081 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     4141 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     4201 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     4261 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     4321 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     4381 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     4441 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     4501 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     4561 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     4621 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     4681 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     4741 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     4801 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     4861 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     4921 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     4981 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     5041 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     5101 ggacccatgc attgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     5161 cagcaggcag aagtatgcaa agcatgcgtc tcaattagtc agcaaccata gtcccgcccc
     5221 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     5281 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     5341 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctttct agaggtacca
     5401 ccggagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     5461 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     5521 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     5581 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgccgt ggcggtctgg
     5641 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     5701 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     5761 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     5821 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     5881 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     5941 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     6001 cccggtgcca gttaacgaat tctagatctt gagacaaatg gcagtattca tccacaattt
     6061 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     6121 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     6181 ttattacagg gacagcagag atccactttg gcgccggctc gaggcctgca ggttgcgcct
     6241 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa
     6301 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg
     6361 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg
     6421 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac
     6481 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc
     6541 tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt
     6601 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc
     6661 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga
     6721 cctctctccc caggggctag catcgattga tcaacaagtt tgtacaaaaa agcaggctta
     6781 atgcctcgtg taaaagcagc tcaagctgga agacagagct ctgcaaagag acatcttgca
     6841 gaacaatttg cagttggaga gataataact gacatggcaa aaaaggaatg gaaagtgggt
     6901 cttccaatag gacagggagg ctttggctgt atatatcttg ctgatatgaa ttcttcagag
     6961 tcagttggca gtgatgcacc ttgtgttgta aaagtggaac ccagtgacaa tggacctctt
     7021 tttactgaat taaagttcta ccaacgagct gcaaaaccag agcaaattca gaaatggatt
     7081 cgtacccgta agctgaagta cctgggagta cccaaatact ggggctctgg tctacatgac
     7141 aaaaatggaa aaagttacag gtttatgata atggatcgct ttgggagtga ccttcagaaa
     7201 atatacgagg ctaacgcaaa gagattttct cggaaaactg tcttgcagct aagcttaaga
     7261 attctggata ttctggaata tattcacgag catgagtatg tgcatggaga tatcaaggcc
     7321 tcaaatcttc ttctgaacta caagaatcct gaccaggtgt acttggtaga ttatggcctt
     7381 gcttatcggt actgcccaga aggagttcat aaagaataca aagaagaccc caaaagatgt
     7441 cacgatggca ctattgaatt cacgagcatc gatgcacaca atggcgtggc cccatcaaga
     7501 cgtggtgatt tggaaatact tggttattgc atgatccaat ggcttactgg ccatcttcct
     7561 tgggaggata atttgaaaga tcctaaatat gttagagatt ccaaaattag atacagagaa
     7621 aatattgcaa gtttgatgga caaatgtttt cctgagaaaa acaaaccagg tgaaattgcc
     7681 aaatacatgg aaacagtgaa attactagac tacactgaaa aacctcttta tgaaaattta
     7741 cgtgacattc ttttgcaagg actaaaagct ataggaagta aggatgatgg caaattggac
     7801 ctcagtgttg tggagaatgg aggtttgaaa gcaaaaacaa taacaaagaa gcgaaagaaa
     7861 gaaattgaag aaagcaagga acctggtgtt gaagatacgg aatggtcaaa cacacagaca
     7921 gaggaggcca tacagacccg ttcaagaacc agaaagagag tccagaagga cccagctttc
     7981 ttgtacaaag tggttaccgg tggagtgcag gtggaaacca tctccccagg agacgggcgc
     8041 accttcccca agcgcggcca gacctgcgtg gtgcactaca ccgggatgct tgaagatgga
     8101 aagaaagttg attcctcccg ggacagaaac aagcccttta agtttatgct aggcaagcag
     8161 gaggtgatcc gaggctggga agaaggggtt gcccagatga gtgtgggtca gagagccaaa
     8221 ctgactatat ctccagatta tgcctatggt gccactgggc acccaggcat catcccacca
     8281 catgccactc tcgtcttcga tgtggagctt ctaaaactgg aaggcggcta cccctacgac
     8341 gtgcccgact acgccggcta tccgtatgat gtcccggact atgcaggcta aacgcgttaa
     8401 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     8461 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     8521 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     8581 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     8641 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     8701 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     8761 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     8821 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     8881 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     8941 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga
     9001 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     9061 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     9121 ggtctctctg gttagaccag atctgagcct gggagct
//
