LOCUS       sequence-394253-        2162 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes pLacHTA1 as a Type 2 part to be used in the Dueber YTK and 
            Ellis MYT systems.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Shaw WM, Khalil AS, Ellis T
  TITLE     A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423.
  PUBMED    37930278
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423."
COMMENT     Sequence Label: pMYT006
FEATURES             Location/Qualifiers
     source          1..2162
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    406..422
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    459..475
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      complement(529..563)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(634..1293)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1208..1227
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1475..2063
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1964..1983
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtcggtctc aaacgaaagg gtgcaacgcg cgaaaaagtg agaacagcct tccctttcgg
       61 gcgacattga gcgtctaacc atagttaacg acccaaccgc gttttcttca aatttgaact
      121 cgccgagctc acaaataatt cattagcgct gttccaaaat tttcgcctca ctgtgcgaag
      181 ctattggaat ggagtgtatt tggtggctca aaaaaagagc acaatagtta actcgtcgtt
      241 gttgaagaaa cgcccgtaga gatatgtggt ttctcatgct gttatttgtt attgcccact
      301 ttgttgattt caaaatcttt tctcaccccc ttccccgttc acgaagccag ccagtggatc
      361 gtaaatacta gcaataagtc ttgacctaaa aaatataaaa gctaattgtg agcggataac
      421 aaacagcact gttgggcctg agtggaggcg ccggtgaatt gtgagcggat aacaagttaa
      481 acacacataa acaaacaaat taaaaaagat ctatgtgaga ccagaccaat aaaaaacgcc
      541 cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct
      601 atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcatcgca
      661 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg
      721 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt
      781 gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact
      841 cacccaggga ttggctgaaa cgaaaaacat attctcaata aaccctttag ggaaataggc
      901 caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc
      961 gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta
     1021 acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc
     1081 cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt
     1141 atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt
     1201 acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc
     1261 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct
     1321 cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc
     1381 tcttacgtgc ccgatcaatc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     1441 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     1501 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     1561 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     1621 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     1681 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     1741 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     1801 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     1861 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     1921 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     1981 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     2041 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     2101 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     2161 gt
//
