LOCUS       sequence-39427-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Zhou P, Bogacki R, McReynolds L, Howley PM
  TITLE     Harnessing the ubiquitination machinery to target the degradation of
            specific cellular proteins.
  JOURNAL   Mol Cell. 2000 Sep . 6(3):751-6.
  PUBMED    11030355
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2000 Sep . 6(3):751-6."
COMMENT     Sequence Label: p4489 Flag-betaTrCP
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      86..95
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             95..118
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggt accgagctcg gatccgccac catggactac aaggacgacg atgacaaaga
      121 tgacccggcc gaggcggtgc tgcaagagaa ggcactcaag tttatgaatt cctcagagag
      181 agaagactgt aataatggcg aaccccctag gaagataata ccagagaaga attcacttag
      241 acagacatac aacagctgtg ccagactctg cttaaaccaa gaaacagtat gtttagcaag
      301 cactgctatg aagactgaga attgtgtggc caaaacaaaa cttgccaatg gcacttccag
      361 tatgattgtg cccaagcaac ggaaactctc agcaagctat gaaaaggaaa aggaactgtg
      421 tgtcaaatac tttgagcagt ggtcagagtc agatcaagtg gaatttgtgg aacatcttat
      481 atcccaaatg tgtcattacc aacatgggca cataaactcg tatcttaaac ctatgttgca
      541 gagagatttc ataactgctc tgccagctcg gggattggat catattgctg agaacattct
      601 gtcatacctg gatgccaaat cactatgtgc tgctgaactt gtgtgcaagg aatggtaccg
      661 agtgacctct gatggcatgc tgtggaagaa gcttatcgag agaatggtca ggacagattc
      721 tctgtggaga ggcctggcgg aacgaagagg atggggacag tatttattca aaaacaaacc
      781 tcctgacggg aatgctcctc ccaactcttt ttatagagc
//
