LOCUS       sequence-394285-        7783 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inserts PDGFRalpha-D842V to activate the MAP kinase pathway..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7783)
  AUTHORS   Carney SV, Banerjee K, Mujeeb A, Zhu B, Haase S, Varela ML, Kadiyala
            P, Tronrud CE, Zhu Z, Mukherji D, Gorla P, Sun Y, Tagett R, Nunez 
            FJ, Luo M, Luo W, Ljungman M, Liu Y, Xia Z, Schwendeman A, Qin T, 
            Sartor MA, Costello JF, Cahill DP, Lowenstein PR, Castro MG
  TITLE     Zinc Finger MYND-Type Containing 8 (ZMYND8) is epigenetically 
            regulated in mutant Isocitrate Dehydrogenase 1 (IDH1) glioma to 
            promote radioresistance.
  JOURNAL   Clin Cancer Res. 2023 Jan 24:CCR-22-1896. doi: 
            10.1158/1078-0432.CCR-22-1896.
  PUBMED    36692427
REFERENCE   2  (bases 1 to 7783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Clin Cancer
            Res. 2023 Jan 24:CCR-22-1896. doi: 10.1158/1078-0432.CCR-22-1896."
COMMENT     Sequence Label: PDGFRalpha-D842V #1
FEATURES             Location/Qualifiers
     source          1..7783
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             888..899
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3958..4079
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3995..4014)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4049..4068
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4509..4526)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4509..4525)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4518..4540)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4734..4753)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4853..4875
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4913..4931)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4999..5103
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5104..5172,5173..5964)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAAKLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5322..5341)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6135..6723
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6624..6643
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6877..6894
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7011..7032
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7047..7052,7053..7070,7071..7077)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7085..7101
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7090..7112
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7109..7125
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7109..7125
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7144..7164
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7146..7164
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagattta
      541 aattctagac caccatgggg acctcccacc aggtctttct ggtcctcagc tgtctcctca
      601 cagggccggg cctcatctcc tgccagctct tattaccctc tatcctccca aacgagaatg
      661 agaagattgt gcagctgaat tcgtcctttt ctctgagatg cgttggggag agtgaagtga
      721 gctggcagca ccccatgtct gaagaagacg accccaacgt ggaaatcaga agtgaggaga
      781 acaacagtgg cctctttgtc acagtgctgg aagtggttaa cgcctccgca gcccacaccg
      841 gatggtacac ttgctactac aaccacactc agacggatga gagtgagatc gaaggcaggc
      901 acatttacat ctatgtacca gacccagaca tggcctttgt gcctctcggg atgaccgatt
      961 ccttagtcat cgtggaagag gatgactctg ccatcatacc ttgccgcacc acagatccgg
     1021 agactcaagt aaccttgcac aataacggga ggctggtgcc tgcctcctat gacagcaggc
     1081 agggcttcaa cggaaccttc agcgtggggc cttacatctg tgaggccacc gtcaaaggga
     1141 ggacgttcaa gaccagcgag tttaatgttt atgccttgaa agcaacgtca gaactgaatc
     1201 tggagatgga cgcacgccag actgtgtata aggcaggaga aacgatcgtg gtgacctgtg
     1261 ccgtctttaa caacgaggtc gttgacctgc agtggactta ccctggagaa gtgagaaaca
     1321 aaggtatcac catgctggag gagatcaaac tcccatccat caaactggtg tacactttga
     1381 ccgtccccaa ggccacggtg aaggacagtg gagagtatga atgtgctgcc cgtcaggcca
     1441 ctaaagaggt caaggaaatg aagagagtca ccatttctgt ccacgagaaa ggcttcgtcg
     1501 agattgagcc cacctttggc cagctggaag ctgtcaactt gcacgaagtc agagagttcg
     1561 tggtggaggt gcaggcctac ccgacgccca ggatatcgtg gctgaaggac aacttgactc
     1621 tgatagagaa tctcaccgag atcaccactg atgtacagaa gagtcaggaa acaaggtatc
     1681 agagcaaatt aaagctgatc cgggctaagg aagaagacag tggccattac accattatag
     1741 ttcaaaatga ggatgacgtg aagagttaca cgtttgagct gtcaacccta gttcctgcat
     1801 ccattttgga tctcgtagat gaccaccatg gctctggcgg gggacagact gtgaggtgta
     1861 cagccgaagg cacccctctt ccagaaatcg actggatgat ctgcaagcat attaagaaat
     1921 gtaataatga cacttcgtgg acagttttgg ccagcaatgt ctcaaatatt atcacagagc
     1981 tccctcgccg tggcaggagt accgtggagg gacgagtgtc cttcgccaaa gtggaagaga
     2041 ccatcgcagt tcgatgcctg gcaaagaaca acctcagcgt tgtggcccgt gagctgaagc
     2101 tggtggctcc cactctgcga tctgaactca cagtggcggc cgcagtgttg gtgctgttgg
     2161 tgattgtcat tgtctctctc atcgtcctgg tggtcatttg gaagcagaaa ccacggtatg
     2221 aaattcgctg gagggttatc gagtcaatca gccccgatgg acatgagtat atctatgtgg
     2281 accccatgca gttgccttac gactccagat gggaattccc gagagatggc ctcgtgcttg
     2341 gtcggatttt gggatccggt gcatttggga aagtggtcga aggtacagct tatggattaa
     2401 gccggtccca acctgtaatg aaggtggctg tgaagatgct caaacccaca gccaggtcta
     2461 gtgaaaagca ggctctcatg tctgagctga agataatgac tcacctgggg ccacatttga
     2521 acattgtgaa cttgctggga gcctgcacca agtcaggtcc catttacatc atcacagaat
     2581 actgcttcta tggggatttg gtcaactact tgcataagaa cagagatagc ttcatgagcc
     2641 aacacccaga gaagccgaag aaagacctgg acatctttgg attgaatcct gcagacgaga
     2701 gcacaagaag ttatgtgatt ttgtcttttg aaaataacgg cgactacatg gacatgaagc
     2761 aagctgatac cacacagtat gtccccatgc ttgaaaggaa agaggtttct aaatactctg
     2821 acatccagag atcgctgtac gatcggccag cctcctacaa gaagaaatcc atgctagact
     2881 cagaagtcaa aaacctcctt tcggacgatg actccgaggg tctgactttg ctggatctat
     2941 tgagcttcac ctatcaagtt gctcgaggaa tggaattttt ggcttcgaaa aattgtgtcc
     3001 accgggacct ggctgcccgc aacgtcctcc tggcgcaagg aaaaattgtg aagatctgtg
     3061 attttggcct ggccagagtc atcatgcacg attccaacta cgtgtcaaaa ggcagtactt
     3121 tcctgcctgt gaagtggatg gtacccgaga gcatcttcga caacctctac accacgctga
     3181 gtgacgtctg gtcctatggc attctgctct gggaaatctt ttcccttggt ggcacaccct
     3241 atcctggcat gatggtcgat tctactttct acaataagat caagagtgga taccggatgg
     3301 ccaaacctga ccatgccacc agtgaagtct atgagatcat ggtgcagtgc tggaacagtg
     3361 agcccgagaa gagaccctcc ttctaccacc tcagcgagat agtggagaac ctgttgccgg
     3421 gacaatacaa gaagagttat gaaaagattc acctggactt cctaaagagt gaccatccag
     3481 ccgtggcacg catgcgggtg gactctgata atgcgtacat cggtgtcacc tacaaaaatg
     3541 aagaggataa gctgaaggac tgggaaggag gcctggacga acagagactg agcgctgaca
     3601 gtggctacat catccccctg ccagacattg accctgttcc agaggaggaa gacctgggca
     3661 agaggaacag acacagctca cagacttcgg aagagagtgc catcgagaca ggttccagta
     3721 gttccacctt catcaagaga gaggacgaga ccatcgagga catcgacatg atggatgaca
     3781 ttggtataga ttcctcggac ctggtggagg acagcttcct gtaagggccc aagctttggg
     3841 ccgcgactct agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     3901 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     3961 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     4021 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa
     4081 ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     4141 ctcatttttt aaccaatagg acgaaatcgg caaggccgct ctagatggcc agatctagct
     4201 tctaaagcca tgacatcatt ttctggaatt ttccaagctg tttaaaggca cagtcaactt
     4261 agtgtatgta aacttctgac ccactggaat tgtgatacag tgaattataa gtgaaataat
     4321 ctgtctgtaa acaattgttg gaaaaatgac ttgtgtcatg cacaaagtag atgtcctaac
     4381 tgacttgcca aaactattgt ttgttaacaa gaaatttgtg gagtagttga aaaacgagtt
     4441 ttaatgactc caacttaagt gtatgtaaac ttccgacttc aactgtatag ggatccgagc
     4501 tcgaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     4561 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     4621 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
     4681 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
     4741 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     4801 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     4861 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
     4921 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     4981 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     5041 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     5101 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     5161 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     5221 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     5281 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     5341 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     5401 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     5461 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaagttact tctgacaacg
     5521 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     5581 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     5641 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     5701 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     5761 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     5821 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     5881 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     5941 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     6001 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     6061 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     6121 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6181 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     6241 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     6301 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     6361 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     6421 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     6481 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     6541 acggttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6601 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     6661 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6721 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6781 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     6841 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     6901 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     6961 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     7021 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     7081 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     7141 cgcgcaatta accctcacta aagggaacaa aagctgggta ccgggccccc cctcgaggtc
     7201 gactctagct agaggatccc tatacagttg aagtcggaag tttacataca ccttagccaa
     7261 atacatttaa actcactttt tcacaattcc tgacatttaa tcctagtaaa aattccctgt
     7321 cttaggtcag ttaggatcac cactttattt taagaatgtg aaatatcaga ataatagtag
     7381 agagaatgat tcatttcagc ttttatttct ttcatcacat tcccagtggg tcagaagttt
     7441 acatacactc aattagtatt tggtagcatt gcctttaaat tgtttaactt gggtcaaaca
     7501 tttcgagtag ccttccacaa gctgccgtac gggcgcgcca cgcgtatcat atgtaaagct
     7561 gccgtacggg cgcgccacgc gtatcatatg taaagctgcc gtacgggcgc gccacgcgta
     7621 tcatatgtaa agcttgatat ccatggaatt cactagtgcg cgcggccggt tctttcctgc
     7681 gttatcccct gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat
     7741 caattacggg gtcattagtt catagcccat atatggagtt ccg
//
