LOCUS       sequence-394295-        8371 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8371)
  AUTHORS   Kuo MY, Chao M, Taylor J
  TITLE     Initiation of replication of the human hepatitis delta virus genome 
            from cloned DNA: role of delta antigen.
  JOURNAL   J Virol. 1989 May . 63(5):1945-50.
  PUBMED    2649689
REFERENCE   2  (bases 1 to 8371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            1989 May . 63(5):1945-50."
COMMENT     Sequence Label: pSVL(D2m)
FEATURES             Location/Qualifiers
     source          1..8371
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1276..1295
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     polyA_signal    1460..1594
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1497..1516)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1551..1570
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        complement(4973..4991)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4974..4991)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4999..5016)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4999..5015)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5008..5030)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        6314..6671
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6314..6505
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6337..6357)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6522..6657
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7150..7168)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7236..7340
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7341..7409,7410..8201)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7559..7578)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggccttga agctgtccct gatggtcgtc
      661 atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag
      721 aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc
      781 cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc
      841 gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag
      901 gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc
      961 cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt
     1021 catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg
     1081 acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt
     1141 gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc
     1201 cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc
     1261 ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc
     1321 ggtgatgccg gccacgatgc gtccggcgta gaggatcata atcagccata ccacatttgt
     1381 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     1441 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     1501 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     1561 caaactcatc aatgtatctt atcatgtctg gatccccggg taccgagctc gaattctcgg
     1621 aaagaaggat aaggatggag agggggctcc cccggcgaag agggcccgaa cggaccagat
     1681 ggaggtagac tccggacctg ggaagaggcc tctcagggga ggattcaccg acaaggagag
     1741 gcaggatcac cgacgaagga aggccctcga gaacaagaag aagcagctat cggcgggagg
     1801 caagaacctc agcaaggagg aagaagagga actcaggagg ttgaccgagg aagacgagag
     1861 aagggaaaga agagtagccg gcccgccggt tgggggtgtg aaccccctcg aaggtggatc
     1921 gaggggagcg cccgggggcg gcttcgtccc caatctgcag ggagtcccgg agtccccctt
     1981 ctctcggacc ggggaggggc tggacatcag gggaaaccag ggatttccat aggatatact
     2041 cttcccagcc gatccgccct tttctcccca gagttgtcga ccccagtgaa taaagcgggt
     2101 ttccactcac aggtttgcgt ctcgcgtcct tctttcctct tcgggtcggc atggcatctc
     2161 cacctcctcg cggtccgacc tgggcatccg aaggaggacg cacgtccact cggatggcta
     2221 agggagagcc acttttctct cgattctcta tcggaatcta gagagatttg tgggtcccat
     2281 tcgccattac cgaggggacg gtcccctcgg aatgttgccc agccggcgcc agcgaggagg
     2341 ctgggaccat gccggccatc aggtaagaaa ggatggaacg cggaccctgc agagtggggt
     2401 cccgccattc ctgggcgacc cttggggggg gagtcggaat cgagcatcgg gaagggcatc
     2461 ccatggctcc actggtcccc ggtgttccca gcaccccctc cggtcacttt cgaagggggt
     2521 ccggggtccc gctagatggg gacgataagt cgagttcccc gggataagcc tcactcgtcc
     2581 cctctcgggg ggcggaacac ccaccggcta gccccgttgc tttctttgct ttcctcctcg
     2641 cttcggtctc cccctactcc tagcatctcc tcctatcgct atggtcttac tcctaccgct
     2701 cgaagcgcct cttgttcgct gaaggggtcc tctggaggtg atttctctgc tcatctccga
     2761 gtggattcct ccctctgagt gctactcaac ccttcgggcc ggagtgctct ccagatctgg
     2821 agattgggcc cctcggatcg ggaggcccgc ccctttttct tccaccttcc tctttcccct
     2881 tccagagatt ctccggcgtt gtggggatct cgtccgcttc ctaagacctc tctttgcttc
     2941 ttgggagtag tttctccgac gttccaatgc tctttaccgt gacatcccct ctcgggagct
     3001 gatcctcccc ccgcgtctcc tcgctcggaa cttggctcag gctcgggctc gggcggcgag
     3061 tccagcagtc tcctctttac agaaaagagt aagagtactg aggactgccg cctctagccg
     3121 agatgagccg gtccgagtcg aggaagaacc gcggagggag agaagagatc ctcgagcagt
     3181 gggtggccgg aagaaagaag ttagaggaac tcgagagaga cctccggaag acaaagaaga
     3241 aactcaagaa gatagaggac gaaaatccct ggctggggaa catcaggaat tctcggaaag
     3301 aaggataagg atggagaggg ggctcccccg gcgaagaggg cccgaacgga ccagatggag
     3361 gtagactccg gacctgggaa gaggcctctc aggggaggat tcaccgacaa ggagaggcag
     3421 gatcaccgac gaaggaaggc cctcgagaac aagaagaagc agctatcggc gggaggcaag
     3481 aacctcagca aggaggaaga agaggaactc aggaggttga ccgaggaaga cgagagaagg
     3541 gaaagaagag tagccggccc gccggttggg ggtgtgaacc ccctcgaagg tggatcgagg
     3601 ggagcgcccg ggggcggctt cgtccccaat ctgcagggag tcccggagtc ccccttctct
     3661 cggaccgggg aggggctgga catcagggga aaccagggat ttccatagga tatactcttc
     3721 ccagccgatc cgcccttttc tccccagagt tgtcgacccc agtgaataaa gcgggtttcc
     3781 actcacaggt ttgcgtctcg cgtccttctt tcctcttcgg gtcggcatgg catctccacc
     3841 tcctcgcggt ccgacctggg catccgaagg aggacgcacg tccactcgga tggctaaggg
     3901 agagccactt ttctctcgat tctctatcgg aatctagaga gatttgtggg tcccattcgc
     3961 cattaccgag gggacggtcc cctcggaatg ttgcccagcc ggcgccagcg aggaggctgg
     4021 gaccatgccg gccatcaggt aagaaaggat ggaacgcgga ccctgcagag tggggtcccg
     4081 ccattcctgg gcgacccttg gggggggagt cggaatcgag catcgggaag ggcatcccat
     4141 ggctccactg gtccccggtg ttcccagcac cccctccggt cactttcgaa gggggtccgg
     4201 ggtcccgcta gatggggacg ataagtcgag ttccccggga taagcctcac tcgtcccctc
     4261 tcggggggcg gaacacccac cggctagccc cgttgctttc tttgctttcc tcctcgcttc
     4321 ggtctccccc tactcctagc atctcctcct atcgctatgg tcttactcct accgctcgaa
     4381 gcgcctcttg ttcgctgaag gggtcctctg gaggtgattt ctctgctcat ctccgagtgg
     4441 attcctccct ctgagtgcta ctcaaccctt cgggccggag tgctctccag atctggagat
     4501 tgggcccctc ggatcgggag gcccgcccct ttttcttcca ccttcctctt tccccttcca
     4561 gagattctcc ggcgttgtgg ggatctcgtc cgcttcctaa gacctctctt tgcttcttgg
     4621 gagtagtttc tccgacgttc caatgctctt taccgtgaca tcccctctcg ggagctgatc
     4681 ctccccccgc gtctcctcgc tcggaacttg gctcaggctc gggctcgggc ggcgagtcca
     4741 gcagtctcct ctttacagaa aagagtaaga gtactgagga ctgccgcctc tagccgagat
     4801 gagccggtcc gagtcgagga agaaccgcgg agggagagaa gagatcctcg agcagtgggt
     4861 ggccggaaga aagaagttag aggaactcga gagagacctc cggaagacaa agaagaaact
     4921 caagaagata gaggacgaaa atccctggct ggggaacatc aggaattcgt attctatagt
     4981 gtcacctaaa tccaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     5041 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagggtct agactcgagg
     5101 gatcgatccg gtttagagca gaagtaacac ttccgtacag gcctagaagt aaaggcaaca
     5161 tccactgagg agcagttctt tgatttgcac caccaggagc ctcaaatttt tcaataaatt
     5221 cacctgactg cacattagga ctttggcttt gagcttccca cctctcagtt acttgctgaa
     5281 tactgtctgc ttcatcaata ttatcatagg tgtgcccaaa tgatatttgc aacccttccc
     5341 tgttggctac ttgtctcacc attgtaggcc taatgggaga caaagtagag tagtaatctt
     5401 gtaaagagtt ataccaatta acaggagcat taattactgt ccaagtagtt tcctctaaaa
     5461 accttgccaa actgtccctt aaatatcttt gggttcttct ttcaagctcc tgtgaggtga
     5521 gcctaggaat gtcattttgt attacacgcc aaaaagcttg agaaatggca ttaaaaagtg
     5581 ttggacccca atgtctgggg tcaagatact gaacactgtg aacaaaggtt tgtactccag
     5641 gaaataaaat atcataataa tcatctggcc tatacaaatc tacagccatt cctggttgtt
     5701 gatataaacc aacagtagaa actttgtgat cccagtcact aaaaaatcta taccccactt
     5761 gagcaacagc gctcacacca gtcacagttt gcagtaaagc tgcaaatcca gctatagcag
     5821 caggagcccc agatatcaca gcataggcct gtggagtgag gcctatagca gcaattgcct
     5881 cagaggttgt taggccttca acagtagcaa cagatgcaag ttgcacttca attgcagcag
     5941 cggcctctcc agcagcaatt tcagctactg aaaatccagt agcagcagca gcttcagaca
     6001 cagtagcaat taggtccccc aacagtgtta aagcagcacc catggacctg aaataaaaga
     6061 caaaaagact aaacttacca gttaactttc tggtttttca gttaaccttt ctggtttttt
     6121 gcgtttcccg tcaacagtat cttccccttc acaaaattgc agcaaaagct ctaaaacaaa
     6181 cacaaaaagg cgttgagctg tttttttact ttcagtccat gacctacgaa ccttaacgga
     6241 ggcctggcgt gacagccggc gcagcaccat ggcctgaaat aacctctgaa agaggaactt
     6301 ggttaggtac cttctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg
     6361 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     6421 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     6481 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     6541 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     6601 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     6661 gcttttgcaa aaagctttgc aaagatggat aaagttttaa acagagagga atctttgcag
     6721 ctaatggacc ttctaggtct tgaaaggagt gcctggggga atattcctct gatgagaaag
     6781 gcatatttaa aaaaatgcaa ggagtttcat cctgataaag gaggagatga agaaaaaatg
     6841 aagaaaatga atactctgta caagaaaatg gaagatggag taaaatatgc tcatcaacct
     6901 gactttggag gcttctggga tgcaactgag gtatttgctt cttccttaaa tcctggtgtt
     6961 gatgcaatgt actgcaaaca atggcctgag tgtgcaaaga aaatgtctgc taactgcata
     7021 tgcttgctgt gcttactgag gatgaagcat gaaaatagaa aattatacag gaaagatcca
     7081 cttgtgtggg ttgattgcta ctgcttcgat gataagctgt caaacatgag aattcttgaa
     7141 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     7201 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     7261 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     7321 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     7381 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     7441 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     7501 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     7561 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
     7621 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     7681 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     7741 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     7801 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     7861 acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg
     7921 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     7981 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     8041 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     8101 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     8161 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     8221 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     8281 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     8341 cagaccccgt agaaaagatc aaaggatctt c
//
