LOCUS       sequence-394335-        8877 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Elongation reporter construct to quantify elongation duration of 
            4_CGA stalling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8877)
  AUTHORS   Hou W, Harjono V, Harvey AT, Subramaniam AR, Zid BM
  TITLE     Quantification of elongation stalls and impact on gene expression in
            yeast.
  JOURNAL   RNA. 2023 Dec;29(12):1928-1938. doi: 10.1261/rna.079663.123. Epub 
            2023 Oct 2.
  PUBMED    37783489
REFERENCE   2  (bases 1 to 8877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.079663.123"; journalName: "RNA"; date: "2023-12"; 
            volume: "29"; issue: "12"; pages: "1928-1938"
COMMENT     Sequence Label: pAG306-pTet07-synYFP[TTG/AGA]-4xArg[CGA]-nLuc
FEATURES             Location/Qualifiers
     source          1..8877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        194..409
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             410..1213
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(1344..1799)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1481..1502)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1693..1712
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1925..1947
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1939..1956
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1940..1956
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1963..1982
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1963..1981
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        2034..2430
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             join(2443..3063,3064..3450)
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MPRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTI
                     EGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHA
                     DALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(2516..2535)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     terminator      3471..3658
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3526..3550)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    join(3716..3734,3735..3757,3758..3776,3777..3799,3800..3818,
                     3819..3841,3842..3860,3861..3883,3884..3902,3903..3925,
                     3926..3944,3945..3967,3968..3986)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     primer_bind     complement(5439..5457)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     terminator      5469..5658
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        complement(5707..5725)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5746..5762)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5746..5762)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5759..5781)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5770..5786
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5794..5800,5801..5818,5819..5824))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5839..5860
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5977..5994)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6148..6736)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6228..6247)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6907..7698,7699..7767))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7530..7549
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7768..7872)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8850..8868
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac cttttcaatt catcattttt tttttattct tttttttgat ttcggtttcc
      241 ttgaaatttt tttgattcgg taatctccga acagaaggaa gaacgaagga aggagcacag
      301 acttagattg gtatatatac gcatatgtag tgttgaagaa acatgaaatt gcccagtatt
      361 cttaacccaa ctgcacagaa caaaaacctg caggaaacga agataaatca tgtcgaaagc
      421 tacatataag gaacgtgctg ctactcatcc tagtcctgtt gctgccaagc tatttaatat
      481 catgcacgaa aagcaaacaa acttgtgtgc ttcattggat gttcgtacca ccaaggaatt
      541 actggagtta gttgaagcat taggtcccaa aatttgttta ctaaaaacac atgtggatat
      601 cttgactgat ttttccatgg agggcacagt taagccgcta aaggcattat ccgccaagta
      661 caatttttta ctcttcgaag acagaaaatt tgctgacatt ggtaatacag tcaaattgca
      721 gtactctgcg ggtgtataca gaatagcaga atgggcagac attacgaatg cacacggtgt
      781 ggtgggccca ggtattgtta gcggtttgaa gcaggcggca gaagaagtaa caaaggaacc
      841 tagaggcctt ttgatgttag cagaattgtc atgcaagggc tccctatcta ctggagaata
      901 tactaagggt actgttgaca ttgcgaagag cgacaaagat tttgttatcg gctttattgc
      961 tcaaagagac atgggtggaa gagatgaagg ttacgattgg ttgattatga cacccggtgt
     1021 gggtttagat gacaagggag acgcattggg tcaacagtat agaaccgtgg atgatgtggt
     1081 ctctacagga tctgacatta ttattgttgg aagaggacta tttgcaaagg gaagggatgc
     1141 taaggtagag ggtgaacgtt acagaaaagc aggctgggaa gcatatttga gaagatgcgg
     1201 ccagcaaaac taaaaaactg tattataagt aaatgcatgt atactaaact cacaaattag
     1261 agcttcaatt taattatatc agttattacc ctatgcggtg tgaaataccg cacagatgcg
     1321 taaggagaaa ataccgcatc aggaaattgt aagcgttaat attttgttaa aattcgcgtt
     1381 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     1441 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     1501 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     1561 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     1621 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     1681 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     1741 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     1801 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     1861 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     1921 ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgtaatacga ctcactatag
     1981 ggcgaattgg agctcagttt atcattatca atactcgcca tttcaaagaa taccaacttc
     2041 ttttcttttt ttttcttttc tctctccccc gttgttgtct caccatatcc gcaatgacaa
     2101 aaaaatgatg gaagacacta aaggaaaaaa ttaacgacaa agacagcacc aacagatgtc
     2161 gttgttccag agctgatgag gggtatctcg aagcacacga aactttttcc ttccttcatt
     2221 cacgcacact actctctaat gagcaacggt atacggcctt ccttccagtt acttgaattt
     2281 gaaataaaaa aaagtttgct gtcttgctat caagtataaa tagacctgca attattaatc
     2341 ttttgtttcc tcgtcattgt tctcgttccc tttcttcctt gtttcttttt ctgcacaata
     2401 tttcaagcta taccaagcat acaatcaact atctcatcta gaatgcctag attagataaa
     2461 agtaaagtga ttaacagcgc attagagctg cttaatgggg tcggaatcga aggtttaaca
     2521 acccgtaaac tcgcccagaa gctaggtgta gagcagccta cattgtattg gcatgtaaaa
     2581 aataagcggg ctttgctcga cgccttaccc attgagatgt tagataggca ccatactcac
     2641 ttttgccctt tagaagggga aagctggcaa gattttttac gtaataacgc taaaagtttt
     2701 agatgtgctt tactaagtca tcgcgatgga gcaaaagtac atttaggtac acggcctaca
     2761 gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt tatgccaaca aggtttttca
     2821 ctagagaatg cattatatgc actcagcgct gtggggcact ttactttagg ttgcgtattg
     2881 gaagaacaag agcatcaagt cgctaaagaa gaaagggaaa cacctactac tgatagtatg
     2941 ccgccattat tacgacaagc tatcgaatta tttgatcgcc aaggtgcaga gccagccttc
     3001 ttattcggcc ttgaattgat catatgcgga ttagaaaaac aacttaaatg tgaaagtggg
     3061 tccgcgtaca gccgcgcgcg tacgaaaaac aattacgggt ctaccatcga gggcctgctc
     3121 gatctcccgg acgacgacgc ccccgaagag gcggggctgg cggctccgcg cctgtccttt
     3181 ctccccgcgg gacacacgcg cagactgtcg acggcccccc cgaccgatgt cagcctgggg
     3241 gacgagctcc acttagacgg cgaggacgtg gcgatggcgc atgccgacgc gctagacgat
     3301 ttcgatctgg acatgttggg ggacggggat tccccgggtc cgggatttac cccccacgac
     3361 tccgccccct acggcgctct ggatatggcc gacttcgagt ttgagcagat gtttaccgat
     3421 gcccttggaa ttgacgagta cggtgggtag ctcgagccac ttctaaataa gcgaatttct
     3481 tatgatttat gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt
     3541 aaagtgactc ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg
     3601 tcaggttgct ttctcaggta tagtatgagg tcgctcttat tgaccacacc tctaccggca
     3661 gatccgctag ggataacagg gtaatataga tcaattcctc gatcgagttt accactccct
     3721 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
     3781 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
     3841 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
     3901 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
     3961 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gctcggtacc ctatggcatg
     4021 catgtgctct gtatgtatat aaaactcttg ttttcttctt ttctctaaat attctttcct
     4081 tatacattag gtcctttgta gcataaatta ctatacttct atagacacgc aaacacaaat
     4141 acacacacta acttaccgga tcaattatgt ctaagggtga agaattgttc actggtgttg
     4201 ttccaatctt ggttgaattg gacggtgacg ttaacggtca caagttctct gtttctggtg
     4261 aaggtgaagg tgacgctact tacggtaagt tgactttgaa gttcatctgt actactggta
     4321 agttgccagt tccatggcca actttggtta ctactttggg ttacggtttg atgtgtttcg
     4381 ctagataccc agaccacatg aagcaacacg acttcttcaa gtctgctatg ccagaaggtt
     4441 acgttcaaga aagaactatc ttcttcaagg acgacggtaa ctacaagact agagctgaag
     4501 ttaagttcga aggtgacact ttggttaaca gaatcgaatt gaagggtatc gacttcaagg
     4561 aagacggtaa catcttgggt cacaagttgg aatacaacta caactctcac aacgtttaca
     4621 tcatggctga caagcaaaag aacggtatca aggctaactt caagatcaga cacaacatcg
     4681 aagacggttc tgttcaattg gctgaccact accaacaaaa cactccaatc ggtgacggtc
     4741 cagttttgtt gccagacaac cactacttgt cttaccaatc taagttgtct aaggacccaa
     4801 acgaaaagag agaccacatg gttttgttgg aattcgttac tgctgctggt atcactcacg
     4861 gtatggacga attgtacaag cgacgacgac gaatggtttt tactttagaa gattttgttg
     4921 gtgattggcg tcaaactgct ggttataatt tagatcaagt tttagaacaa ggtggtgttt
     4981 cttctttatt tcaaaattta ggtgtttctg ttactcctat tcaacgtatt gttttatctg
     5041 gtgaaaatgg tttaaaaatt gatattcatg ttattattcc ttatgaaggt ttatctggtg
     5101 atcaaatggg tcaaattgaa aaaattttta aagttgttta tcctgttgat gatcatcatt
     5161 ttaaagttat tttacattat ggtactttag ttattgatgg tgttactcct aatatgattg
     5221 attattttgg tcgtccttat gaaggtattg ctgtttttga tggtaaaaaa attactgtta
     5281 ctggtacttt atggaatggt aataaaatta ttgatgaacg tttaattaat cctgatggtt
     5341 ctttattatt tcgtgttact attaatggtg ttactggttg gcgtttatgt gaacgtattt
     5401 tagctaattc tcatggtttt taaaagctta ttagttatgt cacgcttaca ttcacgccct
     5461 ccccccacat ccgctctaac cgaaaaggaa ggagttagac aacctgaagt ctaggtccct
     5521 atttattttt ttatagttat gttagtatta agaacgttat ttatatttca aatttttctt
     5581 ttttttctgt acagacgcgt gtacgcatgt aacattatac tgaaaacctt gcttgagaag
     5641 gttttgggac gctcgaaggc tttaatttgc ggccgtcgag ggggggcccg gtaccagctt
     5701 ttgttccctt tagtgagggt taatttcgag cttggcgtaa tcatggtcat agctgtttcc
     5761 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     5821 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     5881 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     5941 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     6001 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     6061 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     6121 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     6181 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     6241 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     6301 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     6361 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     6421 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     6481 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     6541 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     6601 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     6661 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     6721 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     6781 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     6841 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     6901 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     6961 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     7021 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     7081 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     7141 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     7201 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     7261 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     7321 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     7381 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     7441 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     7501 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     7561 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     7621 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     7681 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     7741 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     7801 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     7861 aggggttccg cgcacatttc cccgaaaagt gccacctggg gggtgctaat tatggcattg
     7921 attggggcgg taaaacattc aaagtcaatg aaaaggatgc taaattgaat gatactgaag
     7981 acatttcatt cgcttctcag atcaaaaact taaatctgtc tatatgagct gtcccaaagc
     8041 cacttcttac ctttcaagaa aacatggacc ttgctttgcc acaaaataat tgcctgtaag
     8101 atagtttttt atttgcacat tgttattact aacctatgct ttgctacgga cgactgcttg
     8161 gtaaaaatgc aaaagtatta ggatgtctca caagaaactg ctcgtggtga aaattttcag
     8221 ccgcgaccat cctcccacgc caacaagccc tcttttgccc atccttctgg aattcgcggt
     8281 gaaaatcaat ttgatatata aaattccctc ataatatcta ggaacgggat cacatatctc
     8341 aatgagctaa agcggccgct tcttcgaatg ggaccagcta aagtgatcaa acttcatgca
     8401 ggatgttgga cactttttct ttcaagaagc tccaaatata taaatttttg caatctagga
     8461 attgaaatat cgtacttccg attcagaata tctcaactct actacatcta tatatgactg
     8521 tactcgcgtt cttggtaaac atcaattagt aggtgttttt gcgagcatat gttgagctca
     8581 gaaaatatga gcacaatctt cgaaaccatt gtttttttgt gcaaaagtca ccatcgggtt
     8641 tatagtatgg actcttgaac gaaccattct gatctgtatt tctctcatat ttactccatt
     8701 tattttttgc tgctctttgc tttacttcaa cacgatgctt ttcctaagta attttttatt
     8761 ttattcacaa tgctccataa ctcttcaggc cttaaacgac catccatggc ccctaagaaa
     8821 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtc
//
