LOCUS       sequence-394365-        4708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GPCR/G protein BRET biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Janicot R, Maziarz M, Park JC, Zhao J, Luebbers A, Green E, 
            Philibert CE, Zhang H, Layne MD, Wu JC, Garcia-Marcos M
  TITLE     Direct interrogation of context-dependent GPCR activity with a 
            universal biosensor platform.
  JOURNAL   Cell. 2024 Feb 16:S0092-8674(24)00065-5. doi: 
            10.1016/j.cell.2024.01.028.
  PUBMED    38412860
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Feb 16:S0092-8674(24)00065-5. doi: 10.1016/j.cell.2024.01.028."
COMMENT     Sequence Label: pLVX-CMV-Galpha-q ONE-GO
FEATURES             Location/Qualifiers
     source          1..4708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        58..361
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        362..565
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     516..536
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     562..586
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(1088..1090,1091..1093,1094..1804)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1133..1154)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1394..1413)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1741..1762
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(2541..3115,3116..3118,3119..3127)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2708..2725)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2935..2954
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3138..3158
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             3591..4106
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     misc_feature    4120..4708
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4173..4193)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4591..4602)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tcgggtttat tacagggaca gcagagatcc agtttatcga taagcttggg agttccgcgt
       61 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      121 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      181 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      241 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      301 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      361 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      421 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      481 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
      541 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
      601 tccacgctgt tttgacctcc atagaagaca ccgactctac tagaggatct atttccggtg
      661 aattcctcga gactagttct agagcggccg cggatccatg actctggagt ccatcatggc
      721 gtgctgcctg agcgaggagg ccaaggaagc ccggaggatc aacgacgaga tcgagcggca
      781 gctgcgcagg gacaagcgcg acgcccgccg ggaactcaag ctgctgctgc tggggacagg
      841 ggagagtggc aagagcacct tcatcaagca gatgaggatc atccacgggt cgggctactc
      901 tgacgaagac aagcgcggct tcaccaagct ggtgtatcag aacatcttca cggccatgca
      961 ggccatgatc agagcgatgg acacactcaa gatcccatac aagtatgaac acaataaggc
     1021 tcatgcacaa ttggttcgag aggttgatgt ggagaaggtg tctgcttttt caggaggtgg
     1081 cggatccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     1141 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     1201 cacctacggc aagctgaccc tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg
     1261 gcccaccctc gtgaccaccc tgggctacgg cctgcagtgc ttcgcccgct accccgacca
     1321 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     1381 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     1441 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     1501 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcaccg ccgacaagca
     1561 gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg gcggcgtgca
     1621 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     1681 caaccactac ctgagctacc agtccaaact gagcaaagac cccaacgaga agcgcgatca
     1741 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     1801 caagtctggc ggtggaggat ccgagaatcc atatgtagat gcaataaaga gcttgtggaa
     1861 tgatcctgga atccaggagt gctacgacag acgacgggaa tatcagttat ctgactctac
     1921 caaatactat ctgaatgact tggaccgtgt agccgaccct gagtacatgc cgacagagca
     1981 agacgtgctt agagttcgag tccccactac agggatcatc gaatacccct ttgacttaca
     2041 aagtgtcatt ttcagaatgg tcgatgtagg gggccaaagg tcagagagaa gaaaatggat
     2101 acactgcttt gaaaatgtca cctccatcat gtttctagta gcgcttagcg aatatgatca
     2161 agttcttgtg gagtcagaca atgagaaccg catggaggag agcaaagcac tctttagaac
     2221 aattatcacc tacccctggt tccagaactc ctctgtgatt ctgttcttaa acaagaaaga
     2281 tcttctagag gagaaaatca tgtattccca cctagtcgac tacttcccag aatatgatgg
     2341 accccagaga gatgcccagg cagctcgaga attcatcctg aaaatgttcg tggacctgaa
     2401 ccccgacagt gacaaaatca tctactccca cttcacgtgc gccacagata ccgagaacat
     2461 ccgcttcgtc tttgcagccg tcaaggacac catcctgcag ctgaacctga aggagtacaa
     2521 tctggtctaa gcggccgccg cccctctccc tccccccccc ctaacgttac tggccgaagc
     2581 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
     2641 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
     2701 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
     2761 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
     2821 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
     2881 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
     2941 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
     3001 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
     3061 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataagcttgc
     3121 cacaacccgt accaaagatg gggagtagca agagcaagac gtcgaattcc ggaatcaagc
     3181 ttaagtacct ggaggaccgg ggcgaggtga cttttgagaa gatcttctcc cagaagctgg
     3241 ggtacctgct tttccgagac ttctgcctga agcacctgga ggaggccaag cccttggtag
     3301 agttctacga ggagatcaag aaatacgaga agctggagac agaggaggag cgcctggtct
     3361 gcagccgaga gatcttcgac acgtacatca tgaaggagct gctggcctgc tcacatcctt
     3421 tctcgaagag cgccattgag cacgtccagg gccatctggt gaagaagcag gtgcctccgg
     3481 atctcttcca gccatatatt gaagaaattt gccagaacct ccgaggagac gtgttccaga
     3541 aattcatcga gagcgataaa ttcacacggt tttgccagtg gaagggatcc atggtcttca
     3601 cactcgaaga tttcgttggg gactggcgac agacagccgg ctacaacctg gaccaagtcc
     3661 ttgaacaggg aggtgtgtcc agtttgtttc agaatctcgg ggtgtccgta actccgatcc
     3721 aaaggattgt cctgagcggt gaaaatgggc tgaagatcga catccatgtc atcatcccgt
     3781 atgaaggtct gagcggcgac caaatgggcc agatcgaaaa aatttttaag gtggtgtacc
     3841 ctgtggatga tcatcacttt aaggtgatcc tgcactatgg cacactggta atcgacgggg
     3901 ttacgccgaa catgatcgac tatttcggac ggccgtatga aggcatcgcc gtgttcgacg
     3961 gcaaaaagat cactgtaaca gggaccctgt ggaacggcaa caaaattatc gacgagcgcc
     4021 tgatcaaccc cgacggctcc ctgctgttcc gagtaaccat caacggagtg accggctggc
     4081 ggctgtgcga acgcattctg gcgtaaacgc gtctggaaca atcaacctct ggattacaaa
     4141 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     4201 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     4261 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     4321 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     4381 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     4441 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     4501 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     4561 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     4621 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     4681 cggatctccc tttgggccgc ctccccgc
//
