LOCUS       sequence-394367-        4974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GPCR/G protein BRET biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Janicot R, Maziarz M, Park JC, Zhao J, Luebbers A, Green E, 
            Philibert CE, Zhang H, Layne MD, Wu JC, Garcia-Marcos M
  TITLE     Direct interrogation of context-dependent GPCR activity with a 
            universal biosensor platform.
  JOURNAL   Cell. 2024 Feb 16:S0092-8674(24)00065-5. doi: 
            10.1016/j.cell.2024.01.028.
  PUBMED    38412860
REFERENCE   2  (bases 1 to 4974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Feb 16:S0092-8674(24)00065-5. doi: 10.1016/j.cell.2024.01.028."
COMMENT     Sequence Label: pLVX-CMV-Galpha-13 ONE-GO
FEATURES             Location/Qualifiers
     source          1..4974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        58..361
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        362..565
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     516..536
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     562..586
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(1406..1425)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1753..1774
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(2591..3165,3166..3168,3169..3177)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2758..2775)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2985..3004
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3188..3208
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             3857..4372
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     misc_feature    4386..4974
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4439..4459)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4857..4868)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tcgggtttat tacagggaca gcagagatcc agtttatcga taagcttggg agttccgcgt
       61 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      121 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      181 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      241 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      301 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      361 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      421 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      481 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
      541 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
      601 tccacgctgt tttgacctcc atagaagaca ccgactctac tagaggatct atttccggtg
      661 aattcctcga gactagttct agagcggccg cggatccatg gcggacttcc tgccgtcgcg
      721 gtccgtgctg tccgtgtgct tccccggctg cctgctgacg agtggcgagg ccgagcagca
      781 acgcaagtcc aaggagatcg acaaatgcct gtctcgggaa aagacctatg tgaagcggct
      841 ggtgaagatc ctgctgctgg gcgcgggcga gagcggcaag tccaccttcc tgaagcagat
      901 gcggatcatc cacgggcagg acttcgacca gcgcgcgcgc gaggagttcc gccccaccat
      961 ctacagcaac gtgatcaaag gtatgagggt gctggttgat gctcgagaga agcttcatat
     1021 tccctgggga gacaactcaa accaacaaca tggagataag atgatgtcgt ttgatacccg
     1081 ggccccgcgg ggatcaggca tggtttcaaa gggagaagag ttgttcacag gggttgtacc
     1141 gattcttgta gaacttgacg gagatgtcaa cgggcacaag tttagtgtaa gtggagaagg
     1201 cgagggcgat gcgacctatg gaaaactgac ccttaaattt atttgtacta cgggtaaatt
     1261 gcccgttccc tggccgacat tggtaactac attcggatat ggtttgatgt gctttgcaag
     1321 gtatccggat cacatgaagc agcatgattt cttcaagtcc gccatgcccg aaggctacgt
     1381 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     1441 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     1501 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     1561 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     1621 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     1681 gctgctgccc gacaaccact acctgagcta ccagtccgcc atgagcaaag accccaacga
     1741 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     1801 ggacgagctg tacaagggat caggcccgcg gatggcagcc caaggaatgg tggaaacaag
     1861 ggttttctta caatatcttc ctgctataag agcattatgg gcagacagcg gcatacagaa
     1921 tgcctatgac cggcgtcgag aatttcaact gggtgaatct gtaaaatatt tcctggataa
     1981 cttggataaa cttggagaac cagagtatat gccaacagaa caagatattc tgcttgccag
     2041 aagacccacc aaaggcatcc atgaatacga ctttgaaata aaaaatgttc ctttcaaaat
     2101 ggttgatgta ggtggtcaga gatcagaaag gaaacgttgg tttgaatgtt tcgacagtgt
     2161 gacatcaata cttttccttg tttcctcaag tgaatttgac caggtgctta tggaagatcg
     2221 actgaccaat cgccttacag agtctctgaa catttttgaa acaatcgtca ataaccgggt
     2281 tttcagcaat gtctccataa ttctgttctt aaacaagaca gacttgcttg aggagaaggt
     2341 gcaaattgtg agcatcaaag actatttcct agaatttgaa ggggatcccc actgcttaag
     2401 agacgtccaa aaattcctgg tggaatgttt ccggaacaaa cgccgggacc agcaacagaa
     2461 gcccttatac caccacttca ccactgctat caacacggag aacatccgcc ttgttttccg
     2521 tgacgtgaag gatactattc tgcatgacaa cctcaagcag cttatgctac agtgatctag
     2581 gcggccgccg cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat
     2641 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
     2701 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
     2761 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
     2821 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
     2881 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
     2941 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
     3001 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
     3061 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
     3121 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataagcttgc cacaacccgt
     3181 accaaagatg gggagtagca agagcaagac gtcgaattcc ggaatcaagc ttctgattat
     3241 tggcccagag gaagattatg acccaggtta tttcaacaat gagagtgaca tcatcttcca
     3301 agatcttgaa aaactgaagt cacatccagc ttacttggta gtttttctac gttacatcct
     3361 ctctcaggca gaccctggcc ccctgctttt ttatttgtgt tcagaagttt atcaacagac
     3421 aaatcccaaa gattcccgaa gtctggggaa agacatctgg aacattttcc tggagaaaaa
     3481 tgcgcctctc agagtgaaga tccctgagat gttgcaggct gaaattgacc tacgcctgcg
     3541 gaacaatgag gaccctcgca atgtgctctg tgaagctcag gaggcagtca tgctggaaat
     3601 ccaggagcag atcaacgact acagatccaa gcgtactctg ggcctgggca gcctctatgg
     3661 tgaaaatgac ctgctaggcc tggatgggga ccctcttcga gaacgccaaa tggctgagaa
     3721 gcagctggct gcccttggag atatcttgtc caaatatgag gaagatcgga gtgcccccat
     3781 ggactttgct gttaatacct ttatgagcca cgctgggatc cgtcttcggg agtctcgagg
     3841 cggatcaggc ggatccatgg tcttcacact cgaagatttc gttggggact ggcgacagac
     3901 agccggctac aacctggacc aagtccttga acagggaggt gtgtccagtt tgtttcagaa
     3961 tctcggggtg tccgtaactc cgatccaaag gattgtcctg agcggtgaaa atgggctgaa
     4021 gatcgacatc catgtcatca tcccgtatga aggtctgagc ggcgaccaaa tgggccagat
     4081 cgaaaaaatt tttaaggtgg tgtaccctgt ggatgatcat cactttaagg tgatcctgca
     4141 ctatggcaca ctggtaatcg acggggttac gccgaacatg atcgactatt tcggacggcc
     4201 gtatgaaggc atcgccgtgt tcgacggcaa aaagatcact gtaacaggga ccctgtggaa
     4261 cggcaacaaa attatcgacg agcgcctgat caaccccgac ggctccctgc tgttccgagt
     4321 aaccatcaac ggagtgaccg gctggcggct gtgcgaacgc attctggcgt aaacgcgtct
     4381 ggaacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     4441 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     4501 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     4561 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     4621 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     4681 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     4741 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     4801 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     4861 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     4921 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgc
//
