LOCUS       sequence-394432-        7972 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline-inducible BAF expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7972)
  AUTHORS   So J, Mabe NW, Englinger B, Chow KH, Moyer SM, Yerrum S, Trissal MC,
            Marques JG, Kwon JJ, Shim B, Pal S, Panditharatna E, Quinn T, 
            Schaefer DA, Jeong D, Mayhew DL, Hwang J, Beroukhim R, Ligon KL, 
            Stegmaier K, Filbin MG, Hahn WC
  TITLE     VRK1 as a synthetic lethal target in VRK2 promoter-methylated 
            cancers of the nervous system.
  JOURNAL   JCI Insight. 2022 Oct 10;7(19):e158755. doi: 
            10.1172/jci.insight.158755.
  PUBMED    36040810
REFERENCE   2  (bases 1 to 7972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "JCI 
            Insight."; date: "2022-10-10"; volume: "7(19):e158755. doi"; pages: 
            " 10.1172/jci.insight.158755"
COMMENT     Sequence Label: BAF-S4D-tet-puro
FEATURES             Location/Qualifiers
     source          1..7972
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    181..302
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(218..237)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     272..291
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      342..477
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        477..548
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(549..617,618..1409)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(767..786)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1580..2168
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2069..2088
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2322..2339
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2456..2477
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2492..2497,2498..2515,2516..2522)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2530..2546
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2535..2557
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2554..2570
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2554..2570
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2589..2609
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2591..2609
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2637..2863
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2864..3044
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3091..3216
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3709..3942
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4127..4171
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4384..4501
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4555..4869
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    4563..4581
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4599..4617
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4634..4652
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4670..4688
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4706..4724
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4741..4759
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4777..4795
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4842..4866
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    4890..4914
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5188..5212)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5230..5247
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        5257..5767
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5686..5704
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5777..6373
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5777..5796)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6273..6293
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             6374..6427
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(6428..7045,7046..7051,7052..7087,7088..7090,7091..7126,
                     7127..7129,7130..7165,7166..7174)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(7214..7234)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7632..7643)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             join(7848..7972,1..109)
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
       61 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
      121 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
      181 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
      241 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
      301 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
      361 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
      421 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggccttt
      481 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      541 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      601 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      661 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      721 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      781 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      841 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      901 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      961 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     1021 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     1081 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     1141 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     1201 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     1261 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     1321 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     1381 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1441 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1501 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1561 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1621 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1681 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1741 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1801 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1861 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1921 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1981 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     2041 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     2101 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     2161 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     2221 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     2281 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     2341 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     2401 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2461 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2521 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2581 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta
     2641 gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt
     2701 acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc
     2761 cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca
     2821 ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga
     2881 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     2941 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     3001 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     3061 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     3121 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     3181 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     3241 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     3301 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     3361 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     3421 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     3481 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     3541 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     3601 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     3661 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     3721 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     3781 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     3841 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     3901 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     3961 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     4021 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     4081 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     4141 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     4201 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     4261 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     4321 ccacctccca accccgaggg gacaattctc gacctcgaga caaatggcag tattcatcca
     4381 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     4441 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     4501 tcgggtttat tacagggaca gcagagatcc actttggccg cgaatcgata tgtcgagttt
     4561 actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg
     4621 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt
     4681 gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta
     4741 tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta
     4801 tgtcgaggta ggcgtgtacg gtgggaggcc tatataagca gagctcgttt agtgaaccgt
     4861 cagatcgcct ggagaattgg ctagcatcaa caagtttgta caaaaaagca ggcttcacca
     4921 tgacaaccga ccagaaacat cgtgacttcg tggcagagcc catgggggag aagccagtgg
     4981 ggagcctggc tgggattggt gaagtcctgg gcaagaagct ggaggaaagg ggttttgaca
     5041 aggcctatgt tgtccttggc cagtttctgg tgctaaagaa agatgaagac ctcttccggg
     5101 aatggctgaa agacacttgt ggcgccaacg ccaagcagtc ccgggactgc ttcggatgcc
     5161 ttcgagagtg gtgcgacgcc ttcttgcacc cagctttctt gtacaaagtg gtttagtaat
     5221 gaaccggtcc accaccacca ccaccactaa ggatccgggg ttggggttgc gccttttcca
     5281 aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg ggaaacgcag
     5341 cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca cccggatctt
     5401 cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg
     5461 ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc tcactagtac
     5521 cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc
     5581 caatagcggc tgctcagcag ggcgcgccga gagcagcggc cgggaagggg cggtgcggga
     5641 ggcggggtgt ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg
     5701 caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc
     5761 tccccagcaa ttcaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     5821 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     5881 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     5941 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     6001 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     6061 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     6121 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     6181 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     6241 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     6301 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     6361 caagcccggt gccgaaggta gaggttctct cctcacttgt ggtgatgttg aagaaaaccc
     6421 tggtccaatg tctagactgg acaagagcaa agtcataaac ggagctctgg aattactcaa
     6481 tggtgtcggt atcgaaggcc tgacgacaag gaaactcgct caaaagctgg gagttgagca
     6541 gcctaccctg tactggcacg tgaagaacaa gcgggccctg ctcgatgccc tgccaatcga
     6601 gatgctggac aggcatcata cccacttctg ccccctggaa ggcgagtcat ggcaagactt
     6661 tctgcggaac aacgccaagt cataccgctg tgctctcctc tcacatcgcg acggggctaa
     6721 agtgcatctc ggcacccgcc caacagagaa acagtacgaa accctggaaa atcagctcgc
     6781 gttcctgtgt cagcaaggct tctccctgga gaacgcactg tacgctctgt ccgccgtggg
     6841 ccactttaca ctgggctgcg tattggagga acaggagcat caagtagcaa aagaggaaag
     6901 agagacacct accaccgatt ctatgccccc acttctgaga caagcaattg agctgttcga
     6961 ccggcaggga gccgaacctg ccttcctttt cggcctggaa ctaatcatat gtggcctgga
     7021 gaaacagcta aagtgcgaaa gcggcgggcc gaccgacgcc cttgacgatt ttgacttaga
     7081 catgctccca gccgatgccc ttgacgactt tgaccttgat atgctgcctg ctgacgctct
     7141 tgacgatttt gaccttgaca tgctccccgg gtaactaagt aaggatcgat ccaagatatc
     7201 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     7261 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     7321 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     7381 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     7441 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     7501 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
     7561 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     7621 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     7681 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     7741 cctccccgcc tgtttcgcct cggcgtccgg actagaggta cctttaagac caatgactta
     7801 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     7861 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     7921 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ct
//
