LOCUS       sequence-394433-        7972 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline-inducible BAF expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7972)
  AUTHORS   So J, Mabe NW, Englinger B, Chow KH, Moyer SM, Yerrum S, Trissal MC,
            Marques JG, Kwon JJ, Shim B, Pal S, Panditharatna E, Quinn T, 
            Schaefer DA, Jeong D, Mayhew DL, Hwang J, Beroukhim R, Ligon KL, 
            Stegmaier K, Filbin MG, Hahn WC
  TITLE     VRK1 as a synthetic lethal target in VRK2 promoter-methylated 
            cancers of the nervous system.
  JOURNAL   JCI Insight. 2022 Oct 10;7(19):e158755. doi: 
            10.1172/jci.insight.158755.
  PUBMED    36040810
REFERENCE   2  (bases 1 to 7972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "JCI 
            Insight."; date: "2022-10-10"; volume: "7(19):e158755. doi"; pages: 
            " 10.1172/jci.insight.158755"
COMMENT     Sequence Label: BAF-S4A-tet-puro
FEATURES             Location/Qualifiers
     source          1..7972
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(203..222)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1016..1604
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1505..1524
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1758..1775
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1892..1913
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1928..1933,1934..1951,1952..1958)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1966..1982
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1971..1993
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1990..2006
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1990..2006
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2025..2045
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2027..2045
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2073..2299
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2300..2480
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2527..2652
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3145..3378
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3563..3607
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3820..3937
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3991..4305
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    3999..4017
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4035..4053
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4070..4088
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4106..4124
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4142..4160
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4177..4195
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4213..4231
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4278..4302
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    4326..4350
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4624..4648)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4666..4683
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        4693..5203
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5122..5140
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5213..5809
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5213..5232)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5709..5729
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5810..5863
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5864..6481,6482..6487,6488..6523,6524..6526,6527..6562,
                     6563..6565,6566..6601,6602..6610)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(6650..6670)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7068..7079)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7284..7517
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7589..7710
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7626..7645)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7680..7699
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7750..7885
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7885..7956
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7957..7972,1..53,54..845)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
       61 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
      121 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
      181 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
      241 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
      301 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
      361 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
      421 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
      481 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
      541 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
      601 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
      661 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
      721 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
      781 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
      841 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
      901 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
      961 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     1021 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     1081 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     1141 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     1201 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     1261 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     1321 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     1381 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     1441 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     1501 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     1561 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     1621 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     1681 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     1741 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     1801 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     1861 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     1921 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     1981 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     2041 aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc
     2101 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
     2161 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
     2221 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg
     2281 cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc
     2341 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     2401 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     2461 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     2521 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     2581 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     2641 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     2701 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     2761 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     2821 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     2881 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     2941 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
     3001 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     3061 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     3121 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     3181 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     3241 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     3301 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     3361 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     3421 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     3481 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     3541 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     3601 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     3661 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     3721 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggaca
     3781 attctcgacc tcgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     3841 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     3901 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     3961 agatccactt tggccgcgaa tcgatatgtc gagtttactc cctatcagtg atagagaacg
     4021 tatgtcgagt ttactcccta tcagtgatag agaacgatgt cgagtttact ccctatcagt
     4081 gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta
     4141 ctccctatca gtgatagaga acgtatgtcg agtttatccc tatcagtgat agagaacgta
     4201 tgtcgagttt actccctatc agtgatagag aacgtatgtc gaggtaggcg tgtacggtgg
     4261 gaggcctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag aattggctag
     4321 catcaacaag tttgtacaaa aaagcaggct tcaccatgac aaccgcccag aaacatcgtg
     4381 acttcgtggc agagcccatg ggggagaagc cagtggggag cctggctggg attggtgaag
     4441 tcctgggcaa gaagctggag gaaaggggtt ttgacaaggc ctatgttgtc cttggccagt
     4501 ttctggtgct aaagaaagat gaagacctct tccgggaatg gctgaaagac acttgtggcg
     4561 ccaacgccaa gcagtcccgg gactgcttcg gatgccttcg agagtggtgc gacgccttct
     4621 tgcacccagc tttcttgtac aaagtggttt agtaatgaac cggtccacca ccaccaccac
     4681 cactaaggat ccggggttgg ggttgcgcct tttccaaggc agccctgggt ttgcgcaggg
     4741 acgcggctgc tctgggcgtg gttccgggaa acgcagcggc gccgaccctg ggtctcgcac
     4801 attcttcacg tccgttcgca gcgtcacccg gatcttcgcc gctacccttg tgggcccccc
     4861 ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg
     4921 gacgtgacaa acggaagccg cacgtctcac tagtaccctc gcagacggac agcgccaggg
     4981 agcaatggca gcgcgccgac cgcgatgggc tgtggccaat agcggctgct cagcagggcg
     5041 cgccgagagc agcggccggg aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg
     5101 ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag cctccggagc gcacgtcggc
     5161 agtcggctcc ctcgttgacc gaatcaccga cctctctccc cagcaattca ccatgaccga
     5221 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     5281 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     5341 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     5401 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     5461 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     5521 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     5581 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     5641 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     5701 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     5761 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgccg aaggtagagg
     5821 ttctctcctc acttgtggtg atgttgaaga aaaccctggt ccaatgtcta gactggacaa
     5881 gagcaaagtc ataaacggag ctctggaatt actcaatggt gtcggtatcg aaggcctgac
     5941 gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct accctgtact ggcacgtgaa
     6001 gaacaagcgg gccctgctcg atgccctgcc aatcgagatg ctggacaggc atcataccca
     6061 cttctgcccc ctggaaggcg agtcatggca agactttctg cggaacaacg ccaagtcata
     6121 ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg catctcggca cccgcccaac
     6181 agagaaacag tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc
     6241 cctggagaac gcactgtacg ctctgtccgc cgtgggccac tttacactgg gctgcgtatt
     6301 ggaggaacag gagcatcaag tagcaaaaga ggaaagagag acacctacca ccgattctat
     6361 gcccccactt ctgagacaag caattgagct gttcgaccgg cagggagccg aacctgcctt
     6421 ccttttcggc ctggaactaa tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg
     6481 cgggccgacc gacgcccttg acgattttga cttagacatg ctcccagccg atgcccttga
     6541 cgactttgac cttgatatgc tgcctgctga cgctcttgac gattttgacc ttgacatgct
     6601 ccccgggtaa ctaagtaagg atcgatccaa gatatcgtat tcttaactat gttgctcctt
     6661 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     6721 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     6781 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     6841 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     6901 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     6961 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     7021 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     7081 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     7141 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgtt tcgcctcggc
     7201 gtccggacta gaggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     7261 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     7321 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     7381 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     7441 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     7501 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     7561 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     7621 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     7681 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     7741 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     7801 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     7861 tagtgaggag gcttttttgg aggcctttca aatatgtatc cgctcatgag acaataaccc
     7921 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tt
//
