LOCUS       sequence-394434-       12323 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector carrying the MCP-ADAR2(E488Q) with a nuclear 
            export signal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12323)
  AUTHORS   Katrekar D, Xiang Y, Palmer N, Saha A, Meluzzi D, Mali P
  TITLE     Comprehensive interrogation of the ADAR2 deaminase domain for 
            engineering enhanced RNA editing activity and specificity.
  JOURNAL   Elife. 2022 Jan 19;11. pii: 75555. doi: 10.7554/eLife.75555.
  PUBMED    35044296
REFERENCE   2  (bases 1 to 12323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-01-19"; volume: "11. pii: 75555. doi"; pages: " 
            10.7554/eLife.75555"
COMMENT     Sequence Label: MCP-ADAR2-DD(E488Q)
FEATURES             Location/Qualifiers
     source          1..12323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3..23)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8..337
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      188..323
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     250..269
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        385..432
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             451..825
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    955..1076
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(992..1011)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1046..1065
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1125..1141)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1125..1141)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1138..1160)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1149..1165
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1173..1179,1180..1197,1198..1203))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1218..1239
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1356..1373)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1527..2115)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1607..1626)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2286..3077,3078..3146))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2909..2928
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3147..3251)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3326..3345)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3517..3896
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3898..4096
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4051..4071
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4114..4294
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4341..4466
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4959..5192
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5377..5421
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5719..5836
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5887..6135
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     5887..5907
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6058..6077
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        8017..8092
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        8154..8365
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      8384..8393
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9989..10015
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             10025..10081
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             10082..10678
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     10575..10595
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10694..11282
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10747..10767)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11165..11176)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             11354..11587
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(11613..11630)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11619..11843
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11889..12317
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11976..11995)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12186..12207
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
       61 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
      121 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
      181 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
      241 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
      301 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
      361 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
      421 gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg
      481 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
      541 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
      601 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
      661 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
      721 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
      781 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat
      841 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
      901 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
      961 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     1021 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     1081 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     1141 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     1201 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     1261 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     1321 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     1381 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     1441 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     1501 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     1561 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     1621 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     1681 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     1741 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     1801 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     1861 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     1921 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     1981 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     2041 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     2101 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     2161 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     2221 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     2281 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     2341 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     2401 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     2461 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     2521 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     2581 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     2641 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     2701 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     2761 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     2821 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     2881 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     2941 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     3001 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     3061 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     3121 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     3181 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     3241 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc
     3301 ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     3361 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
     3421 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
     3481 cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt
     3541 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
     3601 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     3661 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     3721 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     3781 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     3841 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
     3901 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
     3961 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     4021 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
     4081 aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga
     4141 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     4201 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     4261 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     4321 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     4381 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     4441 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     4501 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     4561 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     4621 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     4681 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     4741 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     4801 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     4861 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     4921 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     4981 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     5041 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     5101 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     5161 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     5221 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     5281 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     5341 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     5401 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     5461 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     5521 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     5581 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     5641 gacagatcca ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat
     5701 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     5761 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     5821 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggttaattaa
     5881 ggtaccgagg gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt
     5941 agagagataa ttagaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga
     6001 cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac
     6061 tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
     6121 aaaggacgaa acaccggaga gggttgtaaa tgagcacaca aaatacacat gctaaaatat
     6181 tatattctat gacctttata aaatcaacca aaatcttctt tttaataact ttagtatcaa
     6241 taattagaat ttttatgttc ctttttgcaa acttttaata aaaatgagca aaataaaaaa
     6301 acgctagttt tagtaactcg cgttgttttc ttcaccttta ataatagcta ctccaccact
     6361 tgttcctaag cggtcagctc ctgcttcaat cattttttga gcatcttcaa atgttctaac
     6421 tccaccagct gctttaacta aagcattgtc tttaacaact gacttcatta gtttaacatc
     6481 ttcaaatgtt gcacctgatt ttgaaaatcc tgttgatgtt ttaacaaatt ctaatccagc
     6541 ttcaacagct atttcacaag ctttcatgat ttcttctttt gttaataaac aattttccat
     6601 aatacattta acaacatgtg atccagctgc tttttttaca gctttcatgt cttctaaaac
     6661 taattcataa tttttgtctt ttaatgcacc aatatttaat accatatcaa tttctgttgc
     6721 accatcttta attgcttcag aaacttcgaa tgcttttgta gctgttgtgc atgcacctag
     6781 aggaaaacct acaacatttg ttattcctac atttgtgcct tttaataatt ctttacaata
     6841 gcttgttcaa tatgaattaa cacaaactgt tgcaaaatca aattcaattg cttcatcaca
     6901 taattgttta atttcagctt tcgtagcatc ttgttttaat aatgtgtgat ctatatattt
     6961 gtttagtttc attttttctc ctatatattc atttttaatt ttaattcttt aataatttcg
     7021 tctactttaa ctttagcgtt ttgaacagat tcaccaacac ctataaaata aatttttagt
     7081 ttaggttcag ttccacttgg gcgaacagca aatcatgact tatcttctaa ataaaatttt
     7141 agtaagtctt gtcctggcat attatacatt ccatcgatgt agtcttcaac attaacaact
     7201 ttaagtccag caatttgagt taagggtgtt gctctcaatg atttcattaa tggttcaatt
     7261 tttaatttct tttcttctgg tttaaaattc aagtttaaag tgaaagtgta atatgcaccc
     7321 atttctttaa ataaatcttc taaatagtct actaatgttt tattttgttt tttataaaat
     7381 caagcagcct ctgctattaa tatagaagct tgtattccat ctttatctct agctgagtca
     7441 tcaattacat atccataact ttcttcataa gcaaaaacaa aatttaatcc gttatcttct
     7501 tctttagcaa tttctctacc cattcattta aatccagtta aagtttttac aatattaact
     7561 ccatattttt catgagcgat tctatcaccc aaatcacttg ttacaaaact tgaatataga
     7621 gccggatttt ttggaatgct atttaagcgt tttagatttg ataattttca atcaattaaa
     7681 attggtcctg tttgatttcc atctaatctt acaaaatgac catcatgttt tattgccatt
     7741 ccaaatctgt cagcatctgg gtcattcata ataataatat ctgcatcatg tttaatacca
     7801 tattcaagcg gtatttttca tgcaggatca aattctggat ttggatttac aacattttta
     7861 aatgtttcat cttcaaatgc atgctcttca acctcaataa cgttatatcc tgattcacgt
     7921 aatatttttg gggtaaattt agttcctgtt ccattaactg cgctaaaaat aatttttaaa
     7981 tcttttttag cttcttgctc ttttttgtac ctctctgttt tagagctaga aatagcaagt
     8041 taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcttttttga
     8101 attcgctagc taggtcttga aaggagtggg aattggctcc ggtgcccgtc agtgggcaga
     8161 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gatccggtgc
     8221 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt
     8281 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg
     8341 caacgggttt gccgccagaa cacaggaccg gttctagagc gctgccacca tggcgtccaa
     8401 tttcactcag tttgtgctgg ttgacaacgg cgggaccggg gacgttacgg tagccccctc
     8461 aaactttgcc aacggtatag cggagtggat aagcagcaat tctaggagtc aagcatacaa
     8521 agttacatgc agcgtgcgcc aatctagcgc tcagaatcgc aagtacacca ttaaagtaga
     8581 ggtccccaag ggagcctgga gaagctatct taacatggag ttgaccatac caatcttcgc
     8641 taccaactct gactgtgaac tcattgtgaa agccatgcaa ggtctgctca aggatggtaa
     8701 cccaattccg tccgctatcg ctgccaactc tgggatttac gggggcagtg ggagcggtgc
     8761 aggatctggt agtccagctg ggggaggagc accgggtagc ggtggggggt ctcagctcca
     8821 tctgccgcaa gttctggctg atgccgtgag cagactcgtg ctaggcaaat tcggcgacct
     8881 gaccgacaac ttcagcagcc ctcacgccag aagaaaagtc ctggccggcg tggtcatgac
     8941 aaccggcaca gatgtgaagg acgccaaagt gatcagcgtg tccaccggca ccaagtgcat
     9001 caacggcgag tacatgagcg acagaggact ggccctgaac gattgccacg ccgagatcat
     9061 cagcagaaga agcctgctgc ggttcctata cacccagctg gaactgtacc tgaacaacaa
     9121 ggacgaccag aagcggtcca tcttccagaa gtctgagaga ggcggcttcc ggctgaaaga
     9181 aaatgtgcag tttcacctat acatctccac aagcccttgc ggcgacgctc ggatctttag
     9241 cccccacgag cccatcctgg aagaacctgc cgacagacac cccaacagaa aggcccgggg
     9301 ccagctgaga acaaagatcg aaagcggcca gggcacaatc cccgtgcgga gcaatgcctc
     9361 tatccaaaca tgggacggcg tgctgcaagg cgagagactg ctgacaatga gctgcagcga
     9421 caagatcgcc cggtggaatg ttgtgggcat ccagggatct ctgctgagca tcttcgtgga
     9481 acccatctac ttctcgagca tcatcctggg cagcctgtac cacggcgatc acctgagcag
     9541 agccatgtac cagcggatca gcaatatcga ggacctgcct cctctgtata ccctgaacaa
     9601 gccactgctg agcggcatct ccaatgccga agctagacag cctggcaagg cccctaactt
     9661 cagcgtgaac tggacagtgg gggatagcgc catcgaagtg atcaatgcca ccaccggcaa
     9721 agacgaacta ggaagagcca gcagactgtg caagcacgcc ctgtattgca gatggatgag
     9781 agtgcatggg aaagtgcctt ctcatctgct gagaagcaag atcaccaagc ctaacgtgta
     9841 ccacgagagc aagctggccg ctaaagagta ccaggccgcc aaggccagac tgttcaccgc
     9901 ctttattaag gccggactcg gcgcctgggt cgagaagcct acagagcagg atcagttcag
     9961 cctgacacct gggagcggat ccggctctct gccaccattg gaaaggctta cgctgggatc
    10021 cggcgcaaca aacttctctc tgctgaaaca agccggagat gtcgaagaga atcctggacc
    10081 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
    10141 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
    10201 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
    10261 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
    10321 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
    10381 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
    10441 cgcgtggttc ctggccaccg tcggagtctc gcccgaccac cagggcaagg gtctgggcag
    10501 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
    10561 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
    10621 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaac
    10681 gcgttaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
    10741 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
    10801 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
    10861 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
    10921 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
    10981 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
    11041 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
    11101 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
    11161 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
    11221 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
    11281 gcgtcgactt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
    11341 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
    11401 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
    11461 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
    11521 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
    11581 tctagcaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
    11641 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
    11701 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
    11761 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
    11821 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
    11881 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
    11941 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
    12001 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
    12061 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
    12121 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
    12181 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
    12241 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
    12301 aaaatttaac gcgaattaat tct
//
