LOCUS       sequence-394461-       21433 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Wheat GRF4-GIF1 chimera in pBUE411.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21433)
  AUTHORS   Chen Z, Debernardi JM, Dubcovsky J, Gallavotti A
  TITLE     The combination of morphogenic regulators BABY BOOM and GRF-GIF 
            improves maize transformation efficiency
  JOURNAL   bioRxiv 2022.09.02.506370
REFERENCE   2  (bases 1 to 21433)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.09.02.506370"
COMMENT     Sequence Label: pBUE411-GG
FEATURES             Location/Qualifiers
     source          1..21433
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    21..45
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(80..332)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(366..455)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     protein_bind    484..508
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2417..2426
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(2423..2447)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     4651..4673
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4665..4682
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4666..4682
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4749..5128
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
     CDS             5408..6199
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_RNA        6351..6426
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        6730..8722
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             8741..8806
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             8813..8833
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8858..12958
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             8858..12958
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             12871..12879
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             12959..13006
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(13663..13679)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13663..13679)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13676..13698)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13687..13703
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13711..13717,13718..13735,13736..13741))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13756..13777
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        13968..14313
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     14256..14276
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             14364..14915
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     polyA_signal    14922..15096
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    15174..15198
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             15623..16417
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      16504..17092
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     16993..17012
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     17246..17263
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    17278..17418
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(17344..17363)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     17504..17526
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      17762..17956
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(18022..19095)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLNDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(19524..20153)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagtttc
       61 tgcaggcgcg ctaatccccg atctagtaac atagatgaca ccgcgcgcga taatttatcc
      121 tagtttgcgc gctatatttt gttttctatc gcgtattaaa tgtataattg cgggactcta
      181 atcataaaaa cccatctcat aaataacgtc atgcattaca tgttaattat tacatgctta
      241 acgtaattca acagaaatta tatgataatc atcgcaagac cggcaacagg attcaatctt
      301 aagaaacttt attgccaaat gtttgaacga tcggggaaat tcgagctcta agcgctgcac
      361 tgagcagcgt aatctggaac gtcatatgga taggatcctg catagtccgg gacgtcatag
      421 ggatagcccg catagtcagg aacatcgtat gggtaaaaga tgttaattaa cccgctgtta
      481 tcaaccactt tgtacaagaa agctgaacgc tagcttcctt cctcctcggt gcccttgagg
      541 tactccccgt cgccgctgtg cgcgccagag ttggcgccgc gagcgtcggc cgacagcgag
      601 gtcgacccct ccttggcgcc gctgccgccg cggccatagt ccgagaagac gctggcgttc
      661 atcatgctgt tgccagtggc gccgccgccg ccgcccatgc tggcctcacc gtgcaggagg
      721 ttgaacccgg tggtcgcgcc ggaggacatc cccagctggc cgtgcgcggc cgcctggtgc
      781 tgctgctgct gctgctgctg ctggagcggc gacatggcct gctgcgcgta catcatcgac
      841 gaccgcgccg ccatcagcga ctgcggcgac atcatctgcg ccgactgctg ctgcatgtac
      901 cgcggcccgg actgcatcat caggttggac gggtactgag acagcgatgc cgtctgcgga
      961 ggctggctgt cggcgatggc ggcgaggtac atgaggttct gctggagctt agcttggttc
     1021 cgtgcgcact cctccacctt gccgttgttc tggttgtcga ggatggccag gatcagctgc
     1081 ttgttctcat ccaggtactg ctgaatgaga tcagtggtgg cagtggtaga ggaagcgtag
     1141 ccccccatca tgctctggtt catctgcatc aggtgttgct gctgcatggc agcggccgcg
     1201 tacatttcgc cggcgaacag catgccgttg ggcgactggg agctggcggc ggagaagtcg
     1261 gaggacgtca tgggtatcga gatcgacagc tgggtggccg agaaggaggc gacgttcgag
     1321 ttgtcgtcgg tcaggtccga ccacgagtcc ctcgtcttcg gccactcgtc gaagaacggc
     1381 cgcagcgtct ggttctcctg cttcgcggag tcgccgctgc cgaagtcgcc gcaccccggg
     1441 aaggagagcg gctgctgctg ctgctgtccc tcggccttga cgccgctgtt gttgttgctg
     1501 ctgttgttct gacccaggtc actcgttgct ccaagctgag gagagtagct tgagagcggg
     1561 aatgtagtcg tttgagaaga tggcggcagg cgccatgggt tctccacgga ggtgttcatg
     1621 gcttctgtca tgagctgact ctgctcgtct gccaaagatc ttattccata tgcggtatac
     1681 ctgaaatctt tgcatgttcc ggcgccgccg agagccgcgt agggagaggc ggcattgttg
     1741 cccatgtgct gctgaggcgg ccccagcgcg gaggagaacg tgccgggcat ggacatgccg
     1801 ttgttccccc cgcctccacc accgttggtg ccgccgatgg cggggtagag ggagtggttg
     1861 tggaagccgg tggcgagggg cggcacgacg gaggcggccg gcggctggga gtgcgagacg
     1921 agctgcgttt ccacaggctt tcttgaacgg ttgcggccgc ggtgcatgtg gcgctcgcaa
     1981 tacttggagt cggaggcggc ctccttggcg caccgccact tcttgccgtc cgtgcgccgg
     2041 caccggcccg gctccggatc caccttcttg cccaggtacg atccgtaccc gatggcgagg
     2101 gggttgtggt agaagcgggc ggcgagggac tcgatgccgc ggcggatggg gagcacgaga
     2161 tccggcggga cggacacgcc ggccaccagg tacttgtata tgagcgcctg gtgctccagc
     2221 tcctcgtact gcgccgccgt gaagggcacc ggcctcgcca tccacctccc gtccatccgc
     2281 gcctcctccc ccatgccgcc attgccgccg gcggagaagg gggaggagcg gcagaagggg
     2341 aggagggagg cggtggcggt ggcggccggg gaggagcggc ggtcgcctgc cggggaaaga
     2401 gaggcatacg gcatcgccat ggtggcagct tttttgtaca aacttgttga taactctagt
     2461 ctgcagaagt aacaccaaac aacagggtga gcatcgacaa aagaaacagt accaagcaaa
     2521 taaatagcgt atgaaggcag ggctaaaaaa atccacatat agctgctgca tatgccatca
     2581 tccaagtata tcaagatcaa aataattata aaacatactt gtttattata atagataggt
     2641 actcaaggtt agagcatatg aatagatgct gcatatgcca tcatgtatat gcatcagtaa
     2701 aacccacatc aacatgtata cctatcctag atcgatattt ccatccatct taaactcgta
     2761 actatgaaga tgtatgacac acacatacag ttccaaaatt aataaataca ccaggtagtt
     2821 tgaaacagta ttctactccg atctagaacg aatgaacgac cgcccaacca caccacatca
     2881 tcacaaccaa gcgaacaaaa agcatctctg tatatgcatc agtaaaaccc gcatcaacat
     2941 gtatacctat cctagatcga tatttccatc catcatcttc aattcgtaac tatgaatatg
     3001 tatggcacac acatacagat ccaaaattaa taaatccacc aggtagtttg aaacagaatt
     3061 ctactccgat ctagaacgac cgcccaacca gaccacatca tcacaaccaa gacaaaaaaa
     3121 agcatgaaaa gatgacccga caaacaagtg cacggcatat attgaaataa aggaaaaggg
     3181 caaaccaaac cctatgcaac gaaacaaaaa aaatcatgaa atcgatcccg tctgcggaac
     3241 ggctagagcc atcccaggat tccccaaaga gaaacactgg caagttagca atcagaacgt
     3301 gtctgacgta caggtcgcat ccgtgtacga acgctagcag cacggatcta acacaaacac
     3361 ggatctaaca caaacatgaa cagaagtaga actaccgggc cctaaccatg gaccggaacg
     3421 ccgatctaga gaaggtagag aggggggggg ggggaggacg agcggcgtac cttgaagcgg
     3481 rggkgccgac gggkggattt gggggagatc tggttgtgtg tgtgtgcgct ccgaacaaca
     3541 cgaggttggg gaaagagggt gtggaggggg tgtctattta ttacggcggg cgrggraggg
     3601 aaagcgaagg rgcggkggga aaggaatccc ccgtagctgc cggtgccgtg agaggaggag
     3661 gaggccgcct gccgtgccgg ctcacgtctg ccgctccgcc acgcaatttc tggatgccga
     3721 cagcggagca agtccaacgg tggagcggaa ctctcgagag gggtccagag gcagcgacag
     3781 agatgccgtg ccgtctgctt cgcttggccc gacgcgacgc tgctggttcg ctggttggtg
     3841 tccgttagac tcgtcgacgg cgtttaacag gctggcatta tctactcgaa acaagaaaaa
     3901 tgtttcctta gtttttttaa tttcttaaag ggtatttgtt taatttttag tcactttatt
     3961 ttattctatt ttatatctaa attattaaat aaaaaaacta aaatagagtt ttagttttct
     4021 taatttagag gctaaaatag aataaaatag atgtactaaa aaaattagtc tataaaaacc
     4081 attaacccta aaccctaaat ggatgtacta ataaaatgga tgaagtatta tataggtgaa
     4141 gctatttgca aaaaaaaagg agaacacatg cacactaaaa agataaaact gtagagtcct
     4201 gttgtcaaaa tactcaattg tcctttagac catgtctaac tgttcattta tatgattctc
     4261 taaaacactg atattattgt agtactatag attatattat tcgtagagta aagtttaaat
     4321 atatgtataa agatagataa actgcacttc aaacaagtgt gacaaaaaaa atatgtggta
     4381 attttttata acttagacat gcaatgctca ttatctctag agaggggcac gaccgggtca
     4441 cgctgcacgc gcctgcagat ccccgggtac cgagctcaaa ctgaaggcgg gaaacgacaa
     4501 tctgatccaa gctcaagctg ctctagcatt cgccattcag gctgcgcaac tgttgggaag
     4561 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     4621 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     4681 gtgccaagct tagtaattca tccaggtctc caagttctag gattttcaga actgcaactt
     4741 attttatcaa ggaatcttta aacatacgaa cagatcactt aaagttcttc tgaagcaact
     4801 taaagttatc aggcatgcat ggatcttgga ggaatcagat gtgcagtcag ggaccatagc
     4861 acaagacagg cgtcttctac tggtgctacc agcaaatgct ggaagccggg aacactgggt
     4921 acgttggaaa ccacgtgatg tgaagaagta agataaactg taggagaaaa gcatttcgta
     4981 gtgggccatg aagcctttca ggacatgtat tgcagtatgg gccggcccat tacgcaattg
     5041 gacgacaaca aagactagta ttagtaccac ctcggctatc cacatagatc aaagctgatt
     5101 taaaagagtt gtgcagatga tccgtggcgt gagaccaacc cagtggacat aagcctgttc
     5161 ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac
     5221 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt
     5281 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg
     5341 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt
     5401 aaacatcatg ggggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg
     5461 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt
     5521 ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct
     5581 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg
     5641 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc
     5701 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct
     5761 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc
     5821 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc
     5881 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga
     5941 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt
     6001 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc
     6061 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg
     6121 cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac
     6181 caaggtagtc ggcaaataat gtctagctag aaattcgttc aagccgacgc cgcttcgcgg
     6241 cgcggcttaa ctcaagcgtt agatgcacta agcacataat tgctcacagc caaactatca
     6301 ggtcaagtct gcttttatta tttttaagcg tgcataataa gccggtctcg gttttagagc
     6361 tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
     6421 cggtgctttt ttttttcgtt ttgcattgag ttttctccgt cgcatgtttg cagttttatt
     6481 ttccgttttg cattgaaatt tctccgtctc atgtttgcag cgtgttcaaa aagtacgcag
     6541 ctgtatttca cttatttacg gcgccacatt ttcatgccgt ttgtgccaac tatcccgagc
     6601 tagtgaatac agcttggctt cacacaacac tggtgacccg ctgacctgct cgtacctcgt
     6661 accgtcgtac ggcacagcat ttggaattaa agggtgtgat cgatactgct tgctgctaag
     6721 cttgcatgcc tgcagtgcag cgtgacccgg tcgtgcccct ctctagagat aatgagcatt
     6781 gcatgtctaa gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca
     6841 gtttatctat ctttatacat atatttaaac tttactctac gaataatata atctatagta
     6901 ctacaataat atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag
     6961 gacaattgag tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt
     7021 ctcctttttt tttgcaaata gcttcaccta tataatactt catccatttt attagtacat
     7081 ccatttaggg tttagggtta atggttttta tagactaatt tttttagtac atctatttta
     7141 ttctatttta gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata
     7201 atttagatat aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga
     7261 aattaaaaaa actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa
     7321 cgccgtcgac gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag
     7381 cgaagcagac ggcacggcat ctctgtcgct gcctctggac ccctctcgag agttccgctc
     7441 caccgttgga cttgctccgc tgtcggcatc cagaaattgc gtggcggagc ggcagacgtg
     7501 agccggcacg gcaggcggcc tcctcctcct ctcacggcac cggcagctac gggggattcc
     7561 tttcccaccg ctccttcgct ttcccttcct cgcccgccgt aataaataga caccccctcc
     7621 acaccctctt tccccaacct cgtgttgttc ggagcgcaca cacacacaac cagatcyccc
     7681 ccaaatccmc ccgtcggcmc ctccgcttca aggtacgccg ctcgtcctcc cccccccccc
     7741 ctctctacct tctctagatc ggcgttccgg tccatggtta gggcccggta gttctacttc
     7801 tgttcatgtt tgtgttagat ccgtgtttgt gttagatccg tgctgctagc gttcgtacac
     7861 ggatgcgacc tgtacgtcag acacgttctg attgctaact tgccagtgtt tctctttggg
     7921 gaatcctggg atggctctag ccgttccgca gacgggatcg atttcatgat tttttttgtt
     7981 tcgttgcata gggtttggtt tgcccttttc ctttatttca atatatgccg tgcacttgtt
     8041 tgtcgggtca tcttttcatg cttttttttg tcttggttgt gatgatgtgg tctggttggg
     8101 cggtcgttct agatcggagt agaattctgt ttcaaactac ctggtggatt tattaatttt
     8161 ggatctgtat gtgtgtgcca tacatattca tagttacgaa ttgaagatga tggatggaaa
     8221 tatcgatcta ggataggtat acatgttgat gcgggtttta ctgatgcata tacagagatg
     8281 ctttttgttc gcttggttgt gatgatgtgg tgtggttggg cggtcgttca ttcgttctag
     8341 atcggagtag aatactgttt caaactacct ggtgtattta ttaattttgg aactgtatgt
     8401 gtgtgtcata catcttcata gttacgagtt taagatggat ggaaatatcg atctaggata
     8461 ggtatacatg ttgatgtggg ttttactgat gcatatacat gatggcatat gcagcatcta
     8521 ttcatatgct ctaaccttga gtacctatct attataataa acaagtatgt tttataatta
     8581 ttttgatctt gatatacttg gatgatggca tatgcagcag ctatatgtgg atttttttag
     8641 ccctgccttc atacgctatt tatttgcttg gtactgtttc ttttgtcgat gctcaccctg
     8701 ttgtttggtg ttacttctgc agccctaggc ctactagatg gattacaagg accacgacgg
     8761 ggattacaag gaccacgaca ttgattacaa ggatgatgat gacaagatgg ctccgaagaa
     8821 gaagaggaag gttggcatcc acggggtgcc agctgctgac aagaagtact cgatcggcct
     8881 cgatattggg actaactctg ttggctgggc cgtgatcacc gacgagtaca aggtgccctc
     8941 aaagaagttc aaggtcctgg gcaacaccga tcggcattcc atcaagaaga atctcattgg
     9001 cgctctcctg ttcgacagcg gcgagacggc tgaggctacg cggctcaagc gcaccgcccg
     9061 caggcggtac acgcgcagga agaatcgcat ctgctacctg caggagattt tctccaacga
     9121 gatggcgaag gttgacgatt ctttcttcca caggctggag gagtcattcc tcgtggagga
     9181 ggataagaag cacgagcggc atccaatctt cggcaacatt gtcgacgagg ttgcctacca
     9241 cgagaagtac cctacgatct accatctgcg gaagaagctc gtggactcca cagataaggc
     9301 ggacctccgc ctgatctacc tcgctctggc ccacatgatt aagttcaggg gccatttcct
     9361 gatcgagggg gatctcaacc cggacaatag cgatgttgac aagctgttca tccagctcgt
     9421 gcagacgtac aaccagctct tcgaggagaa ccccattaat gcgtcaggcg tcgacgcgaa
     9481 ggctatcctg tccgctaggc tctcgaagtc tcggcgcctc gagaacctga tcgcccagct
     9541 gccgggcgag aagaagaacg gcctgttcgg gaatctcatt gcgctcagcc tggggctcac
     9601 gcccaacttc aagtcgaatt tcgatctcgc tgaggacgcc aagctgcagc tctccaagga
     9661 cacatacgac gatgacctgg ataacctcct ggcccagatc ggcgatcagt acgcggacct
     9721 gttcctcgct gccaagaatc tgtcggacgc catcctcctg tctgatattc tcagggtgaa
     9781 caccgagatt acgaaggctc cgctctcagc ctccatgatc aagcgctacg acgagcacca
     9841 tcaggatctg accctcctga aggcgctggt caggcagcag ctccccgaga agtacaagga
     9901 gatcttcttc gatcagtcga agaacggcta cgctgggtac attgacggcg gggcctctca
     9961 ggaggagttc tacaagttca tcaagccgat tctggagaag atggacggca cggaggagct
    10021 gctggtgaag ctcaatcgcg aggacctcct gaggaagcag cggacattcg ataacggcag
    10081 catcccacac cagattcatc tcggggagct gcacgctatc ctgaggaggc aggaggactt
    10141 ctaccctttc ctcaaggata accgcgagaa gatcgagaag attctgactt tcaggatccc
    10201 gtactacgtc ggcccactcg ctaggggcaa ctcccgcttc gcttggatga cccgcaagtc
    10261 agaggagacg atcacgccgt ggaacttcga ggaggtggtc gacaagggcg ctagcgctca
    10321 gtcgttcatc gagaggatga cgaatttcga caagaacctg ccaaatgaga aggtgctccc
    10381 taagcactcg ctcctgtacg agtacttcac agtctacaac gagctgacta aggtgaagta
    10441 tgtgaccgag ggcatgagga agccggcttt cctgtctggg gagcagaaga aggccatcgt
    10501 ggacctcctg ttcaagacca accggaaggt cacggttaag cagctcaagg aggactactt
    10561 caagaagatt gagtgcttcg attcggtcga gatctctggc gttgaggacc gcttcaacgc
    10621 ctccctgggg acctaccacg atctcctgaa gatcattaag gataaggact tcctggacaa
    10681 cgaggagaat gaggatatcc tcgaggacat tgtgctgaca ctcactctgt tcgaggaccg
    10741 ggagatgatc gaggagcgcc tgaagactta cgcccatctc ttcgatgaca aggtcatgaa
    10801 gcagctcaag aggaggaggt acaccggctg ggggaggctg agcaggaagc tcatcaacgg
    10861 cattcgggac aagcagtccg ggaagacgat cctcgacttc ctgaagagcg atggcttcgc
    10921 gaaccgcaat ttcatgcagc tgattcacga tgacagcctc acattcaagg aggatatcca
    10981 gaaggctcag gtgagcggcc agggggactc gctgcacgag catatcgcga acctcgctgg
    11041 ctcgccagct atcaagaagg ggattctgca gaccgtgaag gttgtggacg agctggtgaa
    11101 ggtcatgggc aggcacaagc ctgagaacat cgtcattgag atggcccggg agaatcagac
    11161 cacgcagaag ggccagaaga actcacgcga gaggatgaag aggatcgagg agggcattaa
    11221 ggagctgggg tcccagatcc tcaaggagca cccggtggag aacacgcagc tgcagaatga
    11281 gaagctctac ctgtactacc tccagaatgg ccgcgatatg tatgtggacc aggagctgga
    11341 tattaacagg ctcagcgatt acgacgtcga tcatatcgtt ccacagtcat tcctgaagga
    11401 tgactccatt gacaacaagg tcctcaccag gtcggacaag aaccggggca agtctgataa
    11461 tgttccttca gaggaggtcg ttaagaagat gaagaactac tggcgccagc tcctgaatgc
    11521 caagctgatc acgcagcgga agttcgataa cctcacaaag gctgagaggg gcgggctctc
    11581 tgagctggac aaggcgggct tcatcaagag gcagctggtc gagacacggc agatcactaa
    11641 gcacgttgcg cagattctcg actcacggat gaacactaag tacgatgaga atgacaagct
    11701 gatccgcgag gtgaaggtca tcaccctgaa gtcaaagctc gtctccgact tcaggaagga
    11761 tttccagttc tacaaggttc gggagatcaa caattaccac catgcccatg acgcgtacct
    11821 gaacgcggtg gtcggcacag ctctgatcaa gaagtaccca aagctcgaga gcgagttcgt
    11881 gtacggggac tacaaggttt acgatgtgag gaagatgatc gccaagtcgg agcaggagat
    11941 tggcaaggct accgccaagt acttcttcta ctctaacatt atgaatttct tcaagacaga
    12001 gatcactctg gccaatggcg agatccggaa gcgccccctc atcgagacga acggcgagac
    12061 gggggagatc gtgtgggaca agggcaggga tttcgcgacc gtcaggaagg ttctctccat
    12121 gccacaagtg aatatcgtca agaagacaga ggtccagact ggcgggttct ctaaggagtc
    12181 aattctgcct aagcggaaca gcgacaagct catcgcccgc aagaaggact gggatccgaa
    12241 gaagtacggc gggttcgaca gccccactgt ggcctactcg gtcctggttg tggcgaaggt
    12301 tgagaagggc aagtccaaga agctcaagag cgtgaaggag ctgctgggga tcacgattat
    12361 ggagcgctcc agcttcgaga agaacccgat cgatttcctg gaggcgaagg gctacaagga
    12421 ggtgaagaag gacctgatca ttaagctccc caagtactca ctcttcgagc tggagaacgg
    12481 caggaagcgg atgctggctt ccgctggcga gctgcagaag gggaacgagc tggctctgcc
    12541 gtccaagtat gtgaacttcc tctacctggc ctcccactac gagaagctca agggcagccc
    12601 cgaggacaac gagcagaagc agctgttcgt cgagcagcac aagcattacc tcgacgagat
    12661 cattgagcag atttccgagt tctccaagcg cgtgatcctg gccgacgcga atctggataa
    12721 ggtcctctcc gcgtacaaca agcaccgcga caagccaatc agggagcagg ctgagaatat
    12781 cattcatctc ttcaccctga cgaacctcgg cgcccctgct gctttcaagt acttcgacac
    12841 aactatcgat cgcaagaggt acacaagcac taaggaggtc ctggacgcga ccctcatcca
    12901 ccagtcgatt accggcctct acgagacgcg catcgacctg tctcagctcg ggggcgacaa
    12961 gcggccagcg gcgacgaaga aggcggggca ggcgaagaag aagaagtgag ctcagagctt
    13021 tcgttcgtat catcggtttc gacaacgttc gtcaagttca atgcatcagt ttcattgcgc
    13081 acacaccaga atcctactga gtttgagtat tatggcattg ggaaaactgt ttttcttgta
    13141 ccatttgttg tgcttgtaat ttactgtgtt ttttattcgg ttttcgctat cgaactgtga
    13201 aatggaaatg gatggagaag agttaatgaa tgatatggtc cttttgttca ttctcaaatt
    13261 aatattattt gttttttctc ttatttgttg tgtgttgaat ttgaaattat aagagatatg
    13321 caaacatttt gttttgagta aaaatgtgtc aaatcgtggc ctctaatgac cgaagttaat
    13381 atgaggagta aaacacttgt agttgtacca ttatgcttat tcactaggca acaaatatat
    13441 tttcagacct agaaaagctg caaatgttac tgaatacaag tatgtcctct tgtgttttag
    13501 acatttatga actttccttt atgtaatttt ccagaatcct tgtcagattc taatcattgc
    13561 tttataatta tagttatact catggatttg tagttgagta tgaaaatatt ttttaatgca
    13621 ttttatgact tgccaattga ttgacaacga attcgtaatc atgtcatagc tgtttcctgt
    13681 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
    13741 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
    13801 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
    13861 aggcggtttg cgtattggct agagcagctt gccaacatgg tggagcacga cactctcgtc
    13921 tactccaaga atatcaaaga tacagtctca gaagaccaaa gggctattga gacttttcaa
    13981 caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc
    14041 aaaaggacag tagaaaagga aggtggcacc tacaaatgcc atcattgcga taaaggaaag
    14101 gctatcgttc aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg
    14161 agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat
    14221 atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct
    14281 atataaggaa gttcatttca tttggagagg acacgctgaa atcaccagtc tctctctaca
    14341 aatctatctc tctcgagtct accatgagcc cagaacgacg cccggccgac atccgccgtg
    14401 ccaccgaggc ggacatgccg gcggtctgca ccatcgtcaa ccactacatc gagacaagca
    14461 cggtcaactt ccgtaccgag ccgcaggaac cgcaggagtg gacggacgac ctcgtccgtc
    14521 tgcgggagcg ctatccctgg ctcgtcgccg aggtggacgg cgaggtcgcc ggcatcgcct
    14581 acgcgggccc ctggaaggca cgcaacgcct acgactggac ggccgagtcg accgtgtacg
    14641 tctccccccg ccaccagcgg acgggactgg gctccacgct ctacacccac ctgctgaagt
    14701 ccctggaggc acagggcttc aagagcgtgg tcgctgtcat cgggctgccc aacgacccga
    14761 gcgtgcgcat gcacgaggcg ctcggatatg ccccccgcgg catgctgcgg gcggccggct
    14821 tcaagcacgg gaactggcat gacgtgggtt tctggcagct ggacttcagc ctgccggtac
    14881 cgccccgtcc ggtcctgccc gtcaccgaga tttgactcga gtttctccat aataatgtgt
    14941 gagtagttcc cagataaggg aattagggtt cctatagggt ttcgctcatg tgttgagcat
    15001 ataagaaacc cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa
    15061 ttcctaaaac caaaatccag tactaaaatc cagatccccc gaattaattc ggcgttaatt
    15121 cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca
    15181 ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa
    15241 tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta
    15301 aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg
    15361 ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg
    15421 ttgctgcctg tgatcaccgc ggtttcaaaa tcggctccgt cgatactatg ttatacgcca
    15481 actttgaaaa caactttgaa aaagctgttt tctggtattt aaggttttag aatgcaagga
    15541 acagtgaatt ggagttcgtc ttgttataat tagcttcttg gggtatcttt aaatactgta
    15601 gaaaagagga aggaaataat aaatggctaa aatgagaata tcaccggaat tgaaaaaact
    15661 gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg tctcctgcta aggtatataa
    15721 gctggtggga gaaaatgaaa acctatattt aaaaatgacg gacagccggt ataaagggac
    15781 cacctatgat gtggaacggg aaaaggacat gatgctatgg ctggaaggaa agctgcctgt
    15841 tccaaaggtc ctgcactttg aacggcatga tggctggagc aatctgctca tgagtgaggc
    15901 cgatggcgtc ctttgctcgg aagagtatga agatgaacaa agccctgaaa agattatcga
    15961 gctgtatgcg gagtgcatca ggctctttca ctccatcgac atatcggatt gtccctatac
    16021 gaatagctta gacagccgct tagccgaatt ggattactta ctgaataacg atctggccga
    16081 tgtggattgc gaaaactggg aagaagacac tccatttaaa gatccgcgcg agctgtatga
    16141 ttttttaaag acggaaaagc ccgaagagga acttgtcttt tcccacggcg acctgggaga
    16201 cagcaacatc tttgtgaaag atggcaaagt aagtggcttt attgatcttg ggagaagcgg
    16261 cagggcggac aagtggtatg acattgcctt ctgcgtccgg tcgatcaggg aggatatcgg
    16321 ggaagaacag tatgtcgagc tattttttga cttactgggg atcaagcctg attgggagaa
    16381 aataaaatat tatattttac tggatgaatt gttttagtac ctagaatgca tgaccaaaat
    16441 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
    16501 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
    16561 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
    16621 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
    16681 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
    16741 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
    16801 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
    16861 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
    16921 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
    16981 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
    17041 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
    17101 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
    17161 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
    17221 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct
    17281 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
    17341 cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt
    17401 gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
    17461 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
    17521 cagaggtttt caccgtcatc accgaaacgc gcgaggcagg gtgccttgat gtgggcgccg
    17581 gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt agattgcctg gccgtaggcc
    17641 agccattttt gagcggccag cggccgcgat aggccgacgc gaagcggcgg ggcgtaggga
    17701 gcgcagcgac cgaagggtag gcgctttttg cagctcttcg gctgtgcgct ggccagacag
    17761 ttatgcacag gccaggcggg ttttaagagt tttaataagt tttaaagagt tttaggcgga
    17821 aaaatcgcct tttttctctt ttatatcagt cacttacatg tgtgaccggt tcccaatgta
    17881 cggctttggg ttcccaatgt acgggttccg gttcccaatg tacggctttg ggttcccaat
    17941 gtacgtgcta tccacaggaa acagaccttt tcgacctttt tcccctgcta gggcaatttg
    18001 ccctagcatc tgctccgtac attaggaacc ggcggatgct tcgccctcga tcaggttgcg
    18061 gtagcgcatg actaggatcg ggccagcctg ccccgcctcc tccttcaaat cgtactccgg
    18121 caggtcattt gacccgatca gcttgcgcac ggtgaaacag aacttcttga actctccggc
    18181 gctgccactg cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct tgcctgcggc
    18241 gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga tcgttcaaaa agtaatcggg
    18301 gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca tccaatcagc
    18361 tagctcgatc tcgatgtact ccggccgccc ggtttcgctc tttacgatct tgtagcggct
    18421 aatcaaggct tcaccctcgg ataccgtcac caggcggccg ttcttggcct tcttcgtacg
    18481 ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt cgtctttctg
    18541 ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg gacggaacac
    18601 gcggccgggc ttgtctccct tcccttcccg gtatcggttc atggattcgg ttagatggga
    18661 aaccgccatc agtaccaggt cgtaatccca cacactggcc atgccggccg gccctgcgga
    18721 aacctctacg tgcccgtctg gaagctcgta gcggatcacc tcgccagctc gtcggtcacg
    18781 cttcgacaga cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg tgcccacgtc
    18841 atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct tcctaatcga
    18901 cggcgcaccg gctgccggcg gttgccggga ttctttgcgg attcgatcag cggccgcttg
    18961 ccacgattca ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg cctgcgcggc
    19021 cttcaacttc tccaccaggt catcacccag cgccgcgccg atttgtaccg ggccggatgg
    19081 tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc cagtgccatt gcagggccgg
    19141 caggcaaccc agccgcttac gcctggccaa ccgcccgttc ctccacacat ggggcattcc
    19201 acggcgtcgg tgcctggttg ttcttgattt tccatgccgc ctcctttagc cgctaaaatt
    19261 catctactca tttattcatt tgctcattta ctctggtagc tgcgcgatgt attcagatag
    19321 cagctcggta atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc
    19381 cgccggcaac tgaaagttga cccgcttcat ggctggcgtg tctgccaggc tggccaacgt
    19441 tgcagccttg ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg tgcttttgct
    19501 cattttctct ttacctcatt aactcaaatg agttttgatt taatttcagc ggccagcgcc
    19561 tggacctcgc gggcagcgtc gccctcgggt tctgattcaa gaacggttgt gccggcggcg
    19621 gcagtgcctg ggtagctcac gcgctgcgtg atacgggact caagaatggg cagctcgtac
    19681 ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg
    19741 acaaaggccg cttgtagcct tccatccgtg acctcaatgc gctgcttaac cagctccacc
    19801 aggtcggcgg tggcccatat gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg
    19861 gctgccttga tcgcggacac agccaagtcc gccgcctggg gcgctccgtc gatcactacg
    19921 aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca
    19981 acggttagcg gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg
    20041 gaatcgacta acagaacatc ggccccggcg agttgcaggg cgcgggctag atgggttgcg
    20101 atggtcgtct tgcctgaccc gcctttctgg ttaagtacag cgataacctt catgcgttcc
    20161 ccttgcgtat ttgtttattt actcatcgca tcatatacgc agcgaccgca tgacgcaagc
    20221 tgttttactc aaatacacat caccttttta gacggcggcg ctcggtttct tcagcggcca
    20281 agctggccgg ccaggccgcc agcttggcat cagacaaacc ggccaggatt tcatgcagcc
    20341 gcacggttga gacgtgcgcg ggcggctcga acacgtaccc ggccgcgatc atctccgcct
    20401 cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt ttcatgcttg
    20461 ttcctcttgg cgttcattct cggcggccgc cagggcgtcg gcctcggtca atgcgtcctc
    20521 acggaaggca ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc gctcaagtgc
    20581 gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc gcctctttca cggtgcggcc
    20641 ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg tagggcgggg
    20701 gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc ggtcgatgat
    20761 tagggaacgc tcgaactcgg caatgccggc gaacacggtc aacaccatgc ggccggccgg
    20821 cgtggtggtg tcggcccacg gctctgccag gctacgcagg cccgcgccgg cctcctggat
    20881 gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta gcctggtcac
    20941 tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg gccagctccg ggcggtcgcg
    21001 cctggtgccg gtgatcttct cggaaaacag cttggtgcag ccggccgcgt gcagttcggc
    21061 ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc
    21121 ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat tctactttat
    21181 gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt
    21241 aacttaggac ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac gtcagaagcc
    21301 gactgcacta tagcagcgga ggggttggat caaagtactt tgatcccgag gggaaccctg
    21361 tggttggcat gcacatacaa atggacgaac ggataaacct tttcacgccc ttttaaatat
    21421 ccgattattc taa
//
