LOCUS       sequence-394464-       11103 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual barcoding cassettes for SSN-seq; Barcode Set 1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11103)
  AUTHORS   Lu X, Lofgren SM, Zhao Y, Mazur PK
  TITLE     Multiplexed transcriptomic profiling of the fate of human CAR T 
            cells in vivo via genetic barcoding with shielded small nucleotides.
  JOURNAL   Nat Biomed Eng. 2023 Aug 31. doi: 10.1038/s41551-023-01085-3.
  PUBMED    37652986
REFERENCE   2  (bases 1 to 11103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Biomed 
            Eng. 2023 Aug 31. doi: 10.1038/s41551-023-01085-3."
COMMENT     Sequence Label: pXL_dSSN1_EF1a-tNGFR-2A-Puro
FEATURES             Location/Qualifiers
     source          1..11103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..199
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     154..174
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             217..397
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    444..569
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1062..1295
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1480..1524
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1822..1939
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1990..2238
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1990..2010
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2161..2180
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     protein_bind    complement(2623..2656)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2660..2680
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        2892..4054
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3123..4045
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4002..4022
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4065..4074
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4911..4967
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4968..5564
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     5461..5481
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5598..6186
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5651..5671)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6069..6080)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6258..6491
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6517..6534)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6523..6747
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6793..7221
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6880..6899)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7090..7111
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7230..7250)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7395..7529
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7456..7475
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7591..7638
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7657..8031
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8161..8282
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8198..8217)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8252..8271
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8331..8347)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8331..8347)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8344..8366)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8355..8371
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8379..8385,8386..8403,8404..8409))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8424..8445
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8562..8579)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8733..9321)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8813..8832)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9492..10283,10284..10352))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10115..10134
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10353..10457)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10532..10551)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10723..11102
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
       61 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      121 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      181 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      241 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      301 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      361 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      421 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      481 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      541 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      601 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      661 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      721 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
      781 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
      841 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
      901 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
      961 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1021 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1081 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1141 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1201 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1261 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1321 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1381 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1441 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1501 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1561 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1621 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1681 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1741 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     1801 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     1861 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     1921 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaat
     1981 taaggtaccg agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct
     2041 gttagagaga taattagaat taatttgact gtaaacacaa agatattagt acaaaatacg
     2101 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
     2161 gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
     2221 tggaaaggac gaaacaccgt gctcgcttcg gcagcacata tactagtcga ctcttaaagg
     2281 tttaagagct aagctggaaa cagcatagca agtttaaata aggctagtcc gttatcaact
     2341 tggccgcttt aaggccggtc ctagcaaggc caagtggcac cgagtcggtg ctctagagcg
     2401 gacttcggtc cgcttttttt tctcgagtga tccgacgcgc catctctagg cccgcgccgg
     2461 ccccctcgca cggacttgtg ggagaagctc ggctactccc ctgccccggt taatttgcat
     2521 ataatatttc ctagtaacta tagaggctta atgtgcgata aaagacagat aatctgttct
     2581 ttttaatact agctacattt tacatgatag gcttggattt ctataacttc gtatagcata
     2641 cattatacga agttataaac agcacaaaag gaaactcacc ctaactgtaa agtaattgtg
     2701 tgttttgaga ctataagtat cccttggaga accaccttgt tggtgctcgc ttcggcagca
     2761 catatactag tcgactctta aagttgccat gtgtatcggt ccgatactct gatgatgggc
     2821 tttaaggccg gtcctagcaa ccatcattca tggcaatcta gagcggactt cggtccgctt
     2881 tttttgaatt cggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
     2941 agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa
     3001 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
     3061 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
     3121 aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg
     3181 tgccttgaat tacttccacc tggctccagt acgtgattct tgatcccgag ctggagccag
     3241 gggcgggcct tgcgctttag gagccccttc gcctcgtgct tgagttgagg cctggcctgg
     3301 gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga
     3361 taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga
     3421 tagtcttgta aatgcgggcc aggatctgca cactggtatt tcggtttttg gggccgcggg
     3481 cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg
     3541 gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct
     3601 cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc
     3661 gtgagcggaa agatggccgc ttcccggccc tgctccaggg ggctcaaaat ggaggacgcg
     3721 gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaggggcct ttccgtcctc
     3781 agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt
     3841 ctggagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt
     3901 tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc
     3961 cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt
     4021 tcaaagtttt tttcttccat ttcaggtgtc gtgaacacgc tagcgccacc atgggggcag
     4081 gtgccactgg ccgcgccatg gacgggccgc gcctgctgct gttgctgctt ctgggggtgt
     4141 cccttggagg tgccaaggag gcatgcccca caggcctgta cacacacagc ggtgagtgct
     4201 gcaaagcctg caacctgggc gagggtgtgg cccagccttg tggagccaac cagaccgtgt
     4261 gtgagccctg cctggacagc gtgacgttct ccgacgtggt gagcgcgacc gagccgtgca
     4321 agccgtgcac cgagtgcgtg gggctccaga gcatgtcggc gccatgcgtg gaggccgacg
     4381 acgccgtgtg ccgctgcgcc tacggctact accaggatga gacgactggg cgctgcgagg
     4441 cgtgccgcgt gtgcgaggcg ggctcgggcc tcgtgttctc ctgccaggac aagcagaaca
     4501 ccgtgtgcga ggagtgcccc gacggcacgt attccgacga ggccaaccac gtggacccgt
     4561 gcctgccctg caccgtgtgc gaggacaccg agcgccagct ccgcgagtgc acacgctggg
     4621 ccgacgccga gtgcgaggag atccctggcc gttggattac acggtccaca cccccagagg
     4681 gctcggacag cacagccccc agcacccagg agcctgaggc acctccagaa caagacctca
     4741 tagccagcac ggtggcaggt gtggtgacca cagtgatggg cagctcccag cccgtggtga
     4801 cccgaggcac caccgacaac ctcatccctg tctattgctc catcctggct gctgtggttg
     4861 tgggtcttgt ggcctacata gccttcaaga ggtggaacag cgggtctggc gccacaaact
     4921 tctctctgct aaagcaagca ggtgatgttg aagaaaaccc cgggcctacc gagtacaagc
     4981 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     5041 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     5101 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     5161 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     5221 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     5281 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     5341 ccaccgtcgg agtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     5401 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     5461 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     5521 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgactcgag taaggatcct
     5581 aatctagata agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     5641 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     5701 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     5761 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     5821 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     5881 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     5941 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     6001 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     6061 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     6121 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     6181 ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta gccacttttt
     6241 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     6301 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     6361 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     6421 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     6481 aatctctagc agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     6541 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     6601 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     6661 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     6721 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     6781 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     6841 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     6901 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     6961 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     7021 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     7081 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     7141 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     7201 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     7261 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     7321 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     7381 gtcagcaacc atagtcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     7441 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     7501 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     7561 tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc
     7621 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt
     7681 ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg
     7741 ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg
     7801 ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg
     7861 cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac
     7921 gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg
     7981 cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta
     8041 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     8101 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     8161 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     8221 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     8281 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     8341 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     8401 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     8461 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     8521 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     8581 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     8641 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     8701 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     8761 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     8821 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     8881 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     8941 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     9001 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     9061 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     9121 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     9181 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     9241 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     9301 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     9361 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     9421 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     9481 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     9541 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     9601 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     9661 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     9721 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     9781 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     9841 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     9901 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     9961 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    10021 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    10081 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    10141 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    10201 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    10261 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    10321 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    10381 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    10441 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga
    10501 gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
    10561 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt
    10621 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc
    10681 gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta
    10741 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
    10801 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
    10861 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
    10921 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
    10981 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
    11041 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
    11101 tgg
//
