LOCUS       sequence-394485-       11343 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BHR plasmid (IncP ori) expressing T7 polymerase under control of the
            lac promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11343)
  AUTHORS   Greenwich JL, Alakavuklar MA, Fuqua C
  TITLE     An Inducible T7 Polymerase System for High-Level Protein Expression 
            in Diverse Gram-Negative Bacteria.
  JOURNAL   Microbiol Resour Announc. 2023 Feb 16;12(2):e0111922. doi: 
            10.1128/mra.01119-22. Epub 2023 Jan 16.
  PUBMED    36645284
REFERENCE   2  (bases 1 to 11343)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microbiol 
            Resour Announc."; date: "2023-02-16"; volume: "12(2):e0111922. doi";
            pages: " 10.1128/mra.01119-22. Epub 2023 Jan 16"
COMMENT     Sequence Label: pJLG039
FEATURES             Location/Qualifiers
     source          1..11343
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    56..77
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(92..97,98..115,116..122)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    130..146
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     135..157
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     154..170
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     154..170
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2916..2933)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2916..2932)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2925..2947)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             3445..4644
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     promoter        4703..4780
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             4781..5863
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(4800..4819)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             complement(7157..8305)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(8577..8948)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            8981..9090
                     /note="incP origin of transfer"
     rep_origin      9243..9874
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
       61 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
      121 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
      181 aattcatcga tatctagatc tcgagctcgc gaaagcttat gaacacgatt aacatcgcta
      241 agaacgactt ctctgacatc gaactggctg ctatcccgtt caacactctg gctgaccatt
      301 acggtgagcg tttagctcgc gaacagttgg cccttgagca tgagtcttac gagatgggtg
      361 aagcacgctt ccgcaagatg tttgagcgtc aacttaaagc tggtgaggtt gcggataacg
      421 ctgccgccaa gcctctcatc actaccctac tccctaagat gattgcacgc atcaacgact
      481 ggtttgagga agtgaaagct aagcgcggca agcgcccgac agccttccag ttcctgcaag
      541 aaatcaagcc ggaagccgta gcgtacatca ccattaagac cactctggct tgcctaacca
      601 gtgctgacaa tacaaccgtt caggctgtag caagcgcaat cggtcgggcc attgaggacg
      661 aggctcgctt cggtcgtatc cgtgaccttg aagctaagca cttcaagaaa aacgttgagg
      721 aacaactcaa caagcgcgta gggcacgtct acaagaaagc atttatgcaa gttgtcgagg
      781 ctgacatgct ctctaagggt ctactcggtg gcgaggcgtg gtcttcgtgg cataaggaag
      841 actctattca tgtaggagta cgctgcatcg agatgctcat tgagtcaacc ggaatggtta
      901 gcttacaccg ccaaaatgct ggcgtagtag gtcaagactc tgagactatc gaactcgcac
      961 ctgaatacgc tgaggctatc gcaacccgtg caggtgcgct ggctggcatc tctccgatgt
     1021 tccaaccttg cgtagttcct cctaagccgt ggactggcat tactggtggt ggctattggg
     1081 ctaacggtcg tcgtcctctg gcgctggtgc gtactcacag taagaaagca ctgatgcgct
     1141 acgaagacgt ttacatgcct gaggtgtaca aagcgattaa cattgcgcaa aacaccgcat
     1201 ggaaaatcaa caagaaagtc ctagcggtcg ccaacgtaat caccaagtgg aagcattgtc
     1261 cggtcgagga catccctgcg attgagcgtg aagaactccc gatgaaaccg gaagacatcg
     1321 acatgaatcc tgaggctctc accgcgtgga aacgtgctgc cgctgctgtg taccgcaagg
     1381 acaaggctcg caagtctcgc cgtatcagcc ttgagttcat gcttgagcaa gccaataagt
     1441 ttgctaacca taaggccatc tggttccctt acaacatgga ctggcgcggt cgtgtttacg
     1501 ctgtgtcaat gttcaacccg caaggtaacg atatgaccaa aggactgctt acgctggcga
     1561 aaggtaaacc aatcggtaag gaaggttact actggctgaa aatccacggt gcaaactgtg
     1621 cgggtgtcga taaggttccg ttccctgagc gcatcaagtt cattgaggaa aaccacgaga
     1681 acatcatggc ttgcgctaag tctccactgg agaacacttg gtgggctgag caagattctc
     1741 cgttctgctt ccttgcgttc tgctttgagt acgctggggt acagcaccac ggcctgagct
     1801 ataactgctc ccttccgctg gcgtttgacg ggtcttgctc tggcatccag cacttctccg
     1861 cgatgctccg agatgaggta ggtggtcgcg cggttaactt gcttcctagt gaaaccgttc
     1921 aggacatcta cgggattgtt gctaagaaag tcaacgagat tctacaagca gacgcaatca
     1981 atgggaccga taacgaagta gttaccgtga ccgatgagaa cactggtgaa atctctgaga
     2041 aagtcaagct gggcactaag gcactggctg gtcaatggct ggcttacggt gttactcgca
     2101 gtgtgactaa gcgttcagtc atgacgctgg cttacgggtc caaagagttc ggcttccgtc
     2161 aacaagtgct ggaagatacc attcagccag ctattgattc cggcaagggt ctgatgttca
     2221 ctcagccgaa tcaggctgct ggatacatgg ctaagctgat ttgggaatct gtgagcgtga
     2281 cggtggtagc tgcggttgaa gcaatgaact ggcttaagtc tgctgctaag ctgctggctg
     2341 ctgaggtcaa agataagaag actggagaga ttcttcgcaa gcgttgcgct gtgcattggg
     2401 taactcctga tggtttccct gtgtggcagg aatacaagaa gcctattcag acgcgcttga
     2461 acctgatgtt cctcggtcag ttccgcttac agcctaccat taacaccaac aaagatagcg
     2521 agattgatgc acacaaacag gagtctggta tcgctcctaa ctttgtacac agccaagacg
     2581 gtagccacct tcgtaagact gtagtgtggg cacacgagaa gtacggaatc gaatcttttg
     2641 cactgattca cgactccttc ggtaccattc cggctgacgc tgcgaacctg ttcaaagcag
     2701 tgcgcgaaac tatggttgac acatatgagt cttgtgatgt actggctgat ttctacgacc
     2761 agttcgctga ccagttgcac gagtctcaat tggacaaaat gccagcactt ccggctaaag
     2821 gtaacttgaa cctccgtgac atcttagagt cggacttcgc gttcgcgtaa ctgcaggtcg
     2881 actctagagg atccccgggt accgagctcg aattcactgg ccgtcgtttt acaacgtcgt
     2941 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     3001 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     3061 aatggcgaat ggcgcctgcc tgaagctgcg ggcattcccg atcagaaatg agcgccagtc
     3121 gtcgtcggct ctcggcaccg aatgcgtatg attctccgcc agcatggctt cggccagtgc
     3181 gtcgagcagc gcccgcttgt tcctgaagtg ccagtaaagc gccggctgct gaacccccaa
     3241 ccgttccgcc agtttgcgtg tcgtcagacc gtctacgccg acctcgttca acaggtccag
     3301 ggcggcacgg atcactgtat tcggctgcaa ctttgtcatg cttgacactt tatcactgat
     3361 aaacataata tgtccaccaa cttatcagtg ataaagaatc cgcgcgttca atcggaccag
     3421 cggaggctgg tccggaggcc agacgtgaaa cccaacatac ccctgatcgt aattctgagc
     3481 actgtcgcgc tcgacgctgt cggcatcggc ctgattatgc cggtgctgcc gggcctcctg
     3541 cgcgatctgg ttcactcgaa cgacgtcacc gcccactatg gcattctgct ggcgctgtat
     3601 gcgttggtgc aatttgcctg cgcacctgtg ctgggcgcgc tgtcggatcg tttcgggcgg
     3661 cggccaatct tgctcgtctc gctggccggc gccactgtcg actacgccat catggcgaca
     3721 gcgcctttcc tttgggttct ctatatcggg cggatcgtgg ccggcatcac cggggcgact
     3781 ggggcggtag ccggcgctta tattgccgat atcactgatg gcgatgagcg cgcgcggcac
     3841 ttcggcttca tgagcgcctg tttcgggttc gggatggtcg cgggacctgt gctcggtggg
     3901 ctgatgggcg gtttctcccc ccacgctccg ttcttcgccg cggcagcctt gaacggcctc
     3961 aatttcctga cgggctgttt ccttttgccg gagtcgcaca aaggcgaacg ccggccgtta
     4021 cgccgggagg ctctcaaccc gctcgcttcg ttccggtggg cccggggcat gaccgtcgtc
     4081 gccgccctga tggcggtctt cttcatcatg caacttgtcg gacaggtgcc ggccgcgctt
     4141 tgggtcattt tcggcgagga tcgctttcac tgggacgcga ccacgatcgg catttcgctt
     4201 gccgcatttg gcattctgca ttcactcgcc caggcaatga tcaccggccc tgtagccgcc
     4261 cggctcggcg aaaggcgggc actcatgctc ggaatgattg ccgacggcac aggctacatc
     4321 ctgcttgcct tcgcgacacg gggatggatg gcgttcccga tcatggtcct gcttgcttcg
     4381 ggtggcatcg gaatgccggc gctgcaagca atgttgtcca ggcaggtgga tgaggaacgt
     4441 caggggcagc tgcaaggctc actggcggcg ctcaccagcc tgacctcgat cgtcggaccc
     4501 ctcctcttca cggcgatcta tgcggcttct ataacaacgt ggaacgggtg ggcatggatt
     4561 gcaggcgctg ccctctactt gctctgcctg ccggcgctgc gtcgcgggct ttggagcggc
     4621 gcagggcaac gagccgatcg ctgatcgtgg aaacgatagg ttgcaattcg cgcgcgaagg
     4681 cgaagcggca tgcatttacg ttgacaccat cgaatggtgc aaaacctttc gcggtatggc
     4741 atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgttata
     4801 cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc
     4861 cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta
     4921 cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc
     4981 cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc
     5041 cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg
     5101 taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc
     5161 gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt
     5221 tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac
     5281 gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg
     5341 cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg
     5401 caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca
     5461 acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga
     5521 tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga
     5581 tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac
     5641 caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact
     5701 ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa
     5761 aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     5821 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     5881 taagttagcg cgaattgcaa cctatgccat gcgggtcaag gcgacttccg gcaagctata
     5941 cgcgccctag gagtgcggtt ggaacgttgg cccagccaga tactcccgat cacgagcagg
     6001 acgccgatga tttgaagcgc actcagcgtc tgatccaaga acaaccatcc tagcaacacg
     6061 gcggtccccg ggctgagaaa gcccagtaag gaaacaactg taggttcgag tcgcgagatc
     6121 ccccggaacc aaaggaagta ggttaaaccc gctccgatca ggccgagcca cgccaggccg
     6181 agaacattgg ttcctgtagg catcgggatt ggcggatcaa acactaaagc tactggaacg
     6241 agcagaagtc ctccggccgc cagttgccag gcggtaaagg tgagcagagg cacgggaggt
     6301 tgccacttgc gggtcagcac ggttccgaac gccatggaaa ccgcccccgc caggcccgct
     6361 gcgacgccga caggatctag cgctgcgttt ggtgtcaaca ccaacagcgc cacgcccgca
     6421 gttccgcaaa tagcccccag gaccgccatc aatcgtatcg ggctacctag cagagcggca
     6481 gagatgaaca cgaccatcag cggctgcaca gcgcctaccg tcgccgcgac cccgcccggc
     6541 aggcggtaga ccgaaataaa caacaagctc cagaatagcg aaatattaag tgcgccgagg
     6601 atgaagatgc gcatccacca gattcccgtt ggaatctgtc ggacgatcat cacgagcaat
     6661 aaacccgccg gcaacgcccg cagcagcata ccggcgaccc ctcggcctcg ctgttcgggc
     6721 tccacgaaaa cgccggacag atgcgccttg tgagcgtcct tggggccgtc ctcctgtttg
     6781 aagaccgaca gcccaatgat ctcgccgtcg atgtaggcgc cgaatgccac ggcatctcgc
     6841 aaccgttcag cgaacgcctc catgggcttt ttctcctcgt gctcgtaaac ggacccgaac
     6901 atctctggag ctttcttcag ggccgacaat cggatctcgc ggaaatcctg cacgtcggcc
     6961 gctccaagcc gtcgaatctg agccttaatc acaattgtca attttaatcc tctgtttatc
     7021 ggcagttcgt agagcgcgcc gtgcgtcccg agcgatactg agcgaagcaa gtgcgtcgag
     7081 cagtgcccgc ttgttcctga aatgccagta aagcgctggc tgctgaaccc ccagccggaa
     7141 ctgaccccac aaggccctag cgtttgcaat gcaccaggtc atcattgacc caggcgtgtt
     7201 ccaccaggcc gctgcctcgc aactcttcgc aggcttcgcc gacctgctcg cgccacttct
     7261 tcacgcgggt ggaatccgat ccgcacatga ggcggaaggt ttccagcttg agcgggtacg
     7321 gctcccggtg cgagctgaaa tagtcgaaca tccgtcgggc cgtcggcgac agcttgcggt
     7381 acttctccca tatgaatttc gtgtagtggt cgccagcaaa cagcacgacg atttcctcgt
     7441 cgatcaggac ctggcaacgg gacgttttct tgccacggtc caggacgcgg aagcggtgca
     7501 gcagcgacac cgattccagg tgcccaacgc ggtcggacgt gaagcccatc gccgtcgcct
     7561 gtaggcgcga caggcattcc tcggccttcg tgtaataccg gccattgatc gaccagccca
     7621 ggtcctggca aagctcgtag aacgtgaagg tgatcggctc gccgataggg gtgcgcttcg
     7681 cgtactccaa cacctgctgc cacaccagtt cgtcatcgtc ggcccgcagc tcgacgccgg
     7741 tgtaggtgat cttcacgtcc ttgttgacgt ggaaaatgac cttgttttgc agcgcctcgc
     7801 gcgggatttt cttgttgcgc gtggtgaaca gggcagagcg ggccgtgtcg tttggcatcg
     7861 ctcgcatcgt gtccggccac ggcgcaatat cgaacaagga aagctgcatt tccttgatct
     7921 gctgcttcgt gtgtttcagc aacgcggcct gcttggcctc gctgacctgt tttgccaggt
     7981 cctcgccggc ggtttttcgc ttcttggtcg tcatagttcc tcgcgtgtcg atggtcatcg
     8041 acttcgccaa acctgccgcc tcctgttcga gacgacgcga acgctccacg gcggccgatg
     8101 gcgcgggcag ggcaggggga gccagttgca cgctgtcgcg ctcgatcttg gccgtagctt
     8161 gctggaccat cgagccgacg gactggaagg tttcgcgggg cgcacgcatg acggtgcggc
     8221 ttgcgatggt ttcggcatcc tcggcggaaa accccgcgtc gatcagttct tgcctgtatg
     8281 ccttccggtc aaacgtccga ttcattcacc ctccttgcgg gattgccccg actcacgccg
     8341 gggcaatgtg cccttattcc tgatttgacc cgcctggtgc cttggtgtcc agataatcca
     8401 ccttatcggc aatgaagtcg gtcccgtaga ccgtctggcc gtccttctcg tacttggtat
     8461 tccgaatctt gccctgcacg aataccagct ccgcgaagtc gctcttcttg atggagcgca
     8521 tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta aaaaagtcat
     8581 ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc ctgcttctct
     8641 tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt gcgcgggtcg
     8701 tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt gatgcgggcc
     8761 agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg gccgacggcc
     8821 agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat cgctcttcgt
     8881 tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg cttggtttca
     8941 tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc tcgcagagca
     9001 ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga acacccgctc
     9061 gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca ccaaggaaag
     9121 tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg atataccgaa
     9181 aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagatccg tcgacccttt
     9241 ccgacgctca ccgggctggt tgccctcgcc gctgggctgg cggccgtcta tggccctgca
     9301 aacgcgccag aaacgccgtc gaagccgtgt gcgagacacc gcggccgccg gcgttgtgga
     9361 tacctcgcgg aaaacttggc cctcactgac agatgagggg cggacgttga cacttgaggg
     9421 gccgactcac ccggcgcggc gttgacagat gaggggcagg ctcgatttcg gccggcgacg
     9481 tggagctggc cagcctcgca aatcggcgaa aacgcctgat tttacgcgag tttcccacag
     9541 atgatgtgga caagcctggg gataagtgcc ctgcggtatt gacacttgag gggcgcgact
     9601 actgacagat gaggggcgcg atccttgaca cttgaggggc agagtgctga cagatgaggg
     9661 gcgcacctat tgacatttga ggggctgtcc acaggcagaa aatccagcat ttgcaagggt
     9721 ttccgcccgt ttttcggcca ccgctaacct gtcttttaac ctgcttttaa accaatattt
     9781 ataaaccttg tttttaacca gggctgcgcc ctgtgcgcgt gaccgcgcac gccgaagggg
     9841 ggtgcccccc cttctcgaac cctcccggcc cgctaacgcg ggcctcccat ccccccaggg
     9901 gctgcgcccc tcggccgcga acggcctcac cccaaaaatg gcaggacaac tggtgagtct
     9961 ggatctgaag ttcattgttg tcatggatga tctggaccga ctggagccat cccaggtggc
    10021 ggaagtgttc aggcttgtgc gtgcagtagc cgatctgccc cgctttaccc atattctctg
    10081 ttatgacagg cagattatca ctcatgccgt tgaacatgcg ctgaatatcg aagatggcag
    10141 ccgttatctc cagaaaatca ttcagcttag ttttaaatta ccccgacctg aagcctttga
    10201 tttacgtaat gaatttcgcc agcgggctga ggctctatat cagcaaatta ataatcaacc
    10261 gccagactct ggaatggtaa gggatctcat cgcggtgact gatacctatg gtgccgcact
    10321 ttcgacgcca cgggaaatcc atcaggccat taattccctg atttttcttt atccggggat
    10381 gcgggatttt gtttatttcc ctgatttgtg cctgcttcag cttatacggg tgacaaaccc
    10441 ggctctgtat gactggacag agcattacct gacagaacgg tccgtgattg aaaccggtca
    10501 gggtatgctt tctgacggag agaaagcaga cttccgggag gggcttatca gatgtatgaa
    10561 gacgttcagg gcatcaaatg cagactcgtt tctgacactt gcagactgga tctatctcat
    10621 ctgcgcaagg cagaacgtga agacggccgc cctggacctc gcccgcgagc gccaggcgca
    10681 cgaggccggc gcgcggaccc gcgccacggc ccacgagcgg acgccgcagc aggagcgcca
    10741 gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc agggcatgaa
    10801 aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag gggatgttgt
    10861 ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga tcaatcgtca
    10921 ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa tccatcgaca
    10981 atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa ggcgtctatc
    11041 gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg ctcgccggca
    11101 tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct gattgtcatc
    11161 agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg aagctgcgcg
    11221 tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg cgcgccatcg
    11281 cggtaggcga gcagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
    11341 aat
//
