LOCUS       sequence-394492-        9278 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of human ERBB2 receptor tyrosine kinase in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9278)
  AUTHORS   Gudernova I, Foldynova-Trantirkova S, Ghannamova BE, Fafilek B, 
            Varecha M, Balek L, Hruba E, Jonatova L, Jelinkova I, Kunova 
            Bosakova M, Trantirek L, Mayer J, Krejci P
  TITLE     One reporter for in-cell activity profiling of majority of protein 
            kinase oncogenes.
  JOURNAL   Elife. 2017 Feb 15;6. pii: e21536. doi: 10.7554/eLife.21536.
  PUBMED    28199182
REFERENCE   2  (bases 1 to 9278)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-02-15"; volume: "6. pii: e21536. doi"; pages: " 
            10.7554/eLife.21536"
COMMENT     Sequence Label: pcDNA3.1-ERBB2-V5/HIS
FEATURES             Location/Qualifiers
     source          1..9278
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(34..53)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        225..604
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        605..808
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     759..779
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     853..872
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        853..871
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4766..4807
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4817..4834
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4857..4874)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4863..5087
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5133..5561
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5220..5239)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5430..5451
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5570..5590)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5575..5904
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5755..5890
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5817..5836
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5971..6765
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6025..6044)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6635..6654
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6941..7062
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6978..6997)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7032..7051
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7111..7127)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7111..7127)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7124..7146)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7135..7151
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7159..7165,7166..7183,7184..7189))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7204..7225
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7342..7359)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7513..8101)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7593..7612)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8272..9063,9064..9132))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8895..8914
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9133..9237)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
       61 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
      121 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
      181 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac
      241 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
      301 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      361 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      421 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      481 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      541 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      601 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      661 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      721 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      781 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      841 gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt taagcttggt
      901 accgagctcg gatccagtac ccttcaccat ggagctggcg gccttgtgcc gctgggggct
      961 cctcctcgcc ctcttgcccc ccggagccgc gagcacccaa gtgtgcaccg gcacagacat
     1021 gaagctgcgg ctccctgcca gtcccgagac ccacctggac atgctccgcc acctctacca
     1081 gggctgccag gtggtgcagg gaaacctgga actcacctac ctgcccacca atgccagcct
     1141 gtccttcctg caggatatcc aggaggtgca gggctacgtg ctcatcgctc acaaccaagt
     1201 gaggcaggtc ccactgcaga ggctgcggat tgtgcgaggc acccagctct ttgaggacaa
     1261 ctatgccctg gccgtgctag acaatggaga cccgctgaac aataccaccc ctgtcacagg
     1321 ggcctcccca ggaggcctgc gggagctgca gcttcgaagc ctcacagaga tcttgaaagg
     1381 aggggtcttg atccagcgga acccccagct ctgctaccag gacacgattt tgtggaagga
     1441 catcttccac aagaacaacc agctggctct cacactgata gacaccaacc gctctcgggc
     1501 ctgccacccc tgttctccga tgtgtaaggg ctcccgctgc tggggagaga gttctgagga
     1561 ttgtcagagc ctgacgcgca ctgtctgtgc cggtggctgt gcccgctgca aggggccact
     1621 gcccactgac tgctgccatg agcagtgtgc tgccggctgc acgggcccca agcactctga
     1681 ctgcctggcc tgcctccact tcaaccacag tggcatctgt gagctgcact gcccagccct
     1741 ggtcacctac aacacagaca cgtttgagtc catgcccaat cccgagggcc ggtatacatt
     1801 cggcgccagc tgtgtgactg cctgtcccta caactacctt tctacggacg tgggatcctg
     1861 caccctcgtc tgccccctgc acaaccaaga ggtgacagca gaggatggaa cacagcggtg
     1921 tgagaagtgc agcaagccct gtgcccgagt gtgctatggt ctgggcatgg agcacttgcg
     1981 agaggtgagg gcagttacca gtgccaatat ccaggagttt gctggctgca agaagatctt
     2041 tgggagcctg gcatttctgc cggagagctt tgatggggac ccagcctcca acactgcccc
     2101 gctccagcca gagcagctcc aagtgtttga gactctggaa gagatcacag gttacctata
     2161 catctcagca tggccggaca gcctgcctga cctcagcgtc ttccagaacc tgcaagtaat
     2221 ccggggacga attctgcaca atggcgccta ctcgctgacc ctgcaagggc tgggcatcag
     2281 ctggctgggg ctgcgctcac tgagggaact gggcagtgga ctggccctca tccaccataa
     2341 cacccacctc tgcttcgtgc acacggtgcc ctgggaccag ctctttcgga acccgcacca
     2401 agctctgctc cacactgcca accggccaga ggacgagtgt gtgggcgagg gcctggcctg
     2461 ccaccagctg tgcgcccgag ggcactgctg gggtccaggg cccacccagt gtgtcaactg
     2521 cagccagttc cttcggggcc aggagtgcgt ggaggaatgc cgagtactgc aggggctccc
     2581 cagggagtat gtgaatgcca ggcactgttt gccgtgccac cctgagtgtc agccccagaa
     2641 tggctcagtg acctgttttg gaccggaggc tgaccagtgt gtggcctgtg cccactataa
     2701 ggaccctccc ttctgcgtgg cccgctgccc cagcggtgtg aaacctgacc tctcctacat
     2761 gcccatctgg aagtttccag atgaggaggg cgcatgccag ccttgcccca tcaactgcac
     2821 ccactcctgt gtggacctgg atgacaaggg ctgccccgcc gagcagagag ccagccctct
     2881 gacgtccatc atctctgcgg tggttggcat tctgctggtc gtggtcttgg gggtggtctt
     2941 tgggatcctc atcaagcgac ggcagcagaa gatccggaag tacacgatgc ggagactgct
     3001 gcaggaaacg gagctggtgg agccgctgac acctagcgga gcgatgccca accaggcgca
     3061 gatgcggatc ctgaaagaga cggagctgag gaaggtgaag gtgcttggat ctggcgcttt
     3121 tggcacagtc tacaagggca tctggatccc tgatggggag aatgtgaaaa ttccagtggc
     3181 catcaaagtg ttgagggaaa acacatcccc caaagccaac aaagaaatct tagacgaagc
     3241 atacgtgatg gctggtgtgg gctccccata tgtctcccgc cttctgggca tctgcctgac
     3301 atccacggtg cagctggtga cacagcttat gccctatggc tgcctcttag accatgtccg
     3361 ggaaaaccgc ggacgcctgg gctcccagga cctgctgaac tggtgtatgc agattgccaa
     3421 ggggatgagc tacctggagg atgtgcggct cgtacacagg gacttggccg ctcggaacgt
     3481 gctggtcaag agtcccaacc atgtcaaaat tacagacttc gggctggctc ggctgctgga
     3541 cattgacgag acagagtacc atgcagatgg gggcaaggtg cccatcaagt ggatggcgct
     3601 ggagtccatt ctccgccggc ggttcaccca ccagagtgat gtgtggagtt atggtgtgac
     3661 tgtgtgggag ctgatgactt ttggggccaa accttacgat gggatcccag cccgggagat
     3721 ccctgacctg ctggaaaagg gggagcggct gccccagccc cccatctgca ccattgatgt
     3781 ctacatgatc atggtcaaat gttggatgat tgactctgaa tgtcggccaa gattccggga
     3841 gttggtgtct gaattctccc gcatggccag ggacccccag cgctttgtgg tcatccagaa
     3901 tgaggacttg ggcccagcca gtcccttgga cagcaccttc taccgctcac tgctggagga
     3961 cgatgacatg ggggacctgg tggatgctga ggagtatctg gtaccccagc agggcttctt
     4021 ctgtccagac cctgccccgg gcgctggggg catggtccac cacaggcacc gcagctcatc
     4081 taccaggagt ggcggtgggg acctgacact agggctggag ccctctgaag aggaggcccc
     4141 caggtctcca ctggcaccct ccgaaggggc tggctccgat gtatttgatg gtgacctggg
     4201 aatgggggca gccaaggggc tgcaaagcct ccccacacat gaccccagcc ctctacagcg
     4261 gtacagtgag gaccccacag tacccctgcc ctctgagact gatggctacg ttgcccccct
     4321 gacctgcagc ccccagcctg aatatgtgaa ccagccagat gttcggcccc agcccccttc
     4381 gccccgagag ggccctctgc ctgctgcccg acctgctggt gccactctgg aaaggcccaa
     4441 gactctctcc ccagggaaga atggggtcgt caaagacgtt tttgcctttg ggggtgccgt
     4501 ggagaacccc gagtacttga caccccaggg aggagctgcc cctcagcccc accctcctcc
     4561 tgccttcagc ccagccttcg acaacctcta ttactgggac caggacccac cagagcgggg
     4621 ggctccaccc agcaccttca aagggacacc tacggcagag aacccagagt acctgggtct
     4681 ggacgtgcca gtgaagggtc aagacaattc tgcagatatc cagcacagtg gcggccgctc
     4741 gagtctagag ggcccgcggt tcgaaggtaa gcctatccct aaccctctcc tcggtctcga
     4801 ttctacgcgt accggtcatc atcaccatca ccattgagtt taaacccgct gatcagcctc
     4861 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     4921 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     4981 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     5041 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     5101 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     5161 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     5221 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     5281 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     5341 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     5401 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     5461 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     5521 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     5581 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     5641 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     5701 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     5761 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     5821 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     5881 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     5941 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     6001 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     6061 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     6121 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     6181 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     6241 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     6301 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     6361 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     6421 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     6481 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     6541 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     6601 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     6661 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     6721 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     6781 tcgcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     6841 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     6901 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     6961 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     7021 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     7081 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     7141 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     7201 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     7261 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     7321 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7381 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7441 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7501 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7561 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7621 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7681 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7741 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7801 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     7861 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     7921 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     7981 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8041 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8101 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8161 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8221 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8281 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8341 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     8401 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     8461 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     8521 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     8581 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     8641 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     8701 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     8761 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     8821 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     8881 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     8941 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9001 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9061 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9121 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9181 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9241 atttccccga aaagtgccac ctgacgtcga cggatcgg
//
