LOCUS       sequence-394495-        8144 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of human DDR1 receptor tyrosine kinase in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8144)
  AUTHORS   Gudernova I, Foldynova-Trantirkova S, Ghannamova BE, Fafilek B, 
            Varecha M, Balek L, Hruba E, Jonatova L, Jelinkova I, Kunova 
            Bosakova M, Trantirek L, Mayer J, Krejci P
  TITLE     One reporter for in-cell activity profiling of majority of protein 
            kinase oncogenes.
  JOURNAL   Elife. 2017 Feb 15;6. pii: e21536. doi: 10.7554/eLife.21536.
  PUBMED    28199182
REFERENCE   2  (bases 1 to 8144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-02-15"; volume: "6. pii: e21536. doi"; pages: " 
            10.7554/eLife.21536"
COMMENT     Sequence Label: pcDNA3.1-DDR1-V5/HIS
FEATURES             Location/Qualifiers
     source          1..8144
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(37..56)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        228..607
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        608..811
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     762..782
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     856..875
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        856..874
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3635..3676
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3686..3703
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3726..3743)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3732..3956
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4002..4430
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4089..4108)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4299..4320
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4439..4459)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4444..4773
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4624..4759
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4686..4705
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4840..5634
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4894..4913)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5504..5523
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5810..5931
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5847..5866)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5901..5920
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5980..5996)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5980..5996)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5993..6015)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6004..6020
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6028..6034,6035..6052,6053..6058))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6073..6094
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6211..6228)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6382..6970)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6462..6481)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7141..7932,7933..8001))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7764..7783
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8002..8106)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata
       61 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
      121 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt
      181 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt
      241 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
      301 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
      361 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
      421 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
      481 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
      541 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
      601 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
      661 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
      721 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
      781 tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac ccactgctta
      841 ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct agttaagctt
      901 ggtaccgagc tcggatccag tacccttcac catgggacca gaggccctgt catctttact
      961 gctgctgctc ttggtggcaa gtggagatgc tgacatgaag ggacattttg atcctgccaa
     1021 gtgccgctat gccctgggca tgcaggaccg gaccatccca gacagtgaca tctctgcttc
     1081 cagctcctgg tcagattcca ctgccgcccg ccacagcagg ttggagagca gtgacgggga
     1141 tggggcctgg tgccccgcag ggtcggtgtt tcccaaggag gaggagtact tgcaggtgga
     1201 tctacaacga ctgcacctgg tggctctggt gggcacccag ggacggcatg ccgggggcct
     1261 gggcaaggag ttctcccgga gctaccggct gcgttactcc cgggatggtc gccgctggat
     1321 gggctggaag gaccgctggg gtcaggaggt gatctcaggc aatgaggacc ctgagggagt
     1381 ggtgctgaag gaccttgggc cccccatggt tgcccgactg gttcgcttct acccccgggc
     1441 tgaccgggtc atgagcgtct gtctgcgggt agagctctat ggctgcctct ggagggatgg
     1501 actcctgtct tacactgccc ctgtggggca gacaatgtat ttatctgagg ccgtgtacct
     1561 caacgactcc acctatgacg gacataccgt gggcggactg cagtatgggg gtctgggcca
     1621 gctggcagat ggtgtggtgg ggctggatga ctttaggaag agtcaggagc tgcgggtctg
     1681 gccaggctat gactatgtgg gatggagcaa ccacagcttc tccagtggct atgtggagat
     1741 ggagtttgag tttgaccggc tgagggcctt ccaggctatg caggtccact gtaacaacat
     1801 gcacacgctg ggagcccgtc tgcctggcgg ggtggaatgt cgcttccggc gtggccctgc
     1861 catggcctgg gagggggagc ccatgcgcca caacctaggg ggcaacctgg gggaccccag
     1921 agcccgggct gtctcagtgc cccttggcgg ccgtgtggct cgctttctgc agtgccgctt
     1981 cctctttgcg gggccctggt tactcttcag cgaaatctcc ttcatctctg atgtggtgaa
     2041 caattcctct ccggcactgg gaggcacctt cccgccagcc ccctggtggc cgcctggccc
     2101 acctcccacc aacttcagca gcttggagct ggagcccaga ggccagcagc ccgtggccaa
     2161 ggccgagggg agcccgaccg ccatcctcat tggctgcctg gtggccatca tcctgctcct
     2221 gctgctcatc attgccctca tgctctggcg gctgcactgg cgcaggctcc tcagcaaggc
     2281 tgaacggagg gtgttggaag aggagctgac ggttcacctc tctgtccctg gggacactat
     2341 cctcatcaac aaccgcccag gtcctagaga gccacccccg taccaggagc cccggcctcg
     2401 tgggaatccg ccccactccg ctccctgtgt ccccaatggc tctgcctaca gtggggacta
     2461 tatggagcct gagaagccag gcgccccgct tctgccccca cctccccaga acagcgtccc
     2521 ccattatgcc gaggctgaca ttgttaccct gcagggcgtc accgggggca acacctatgc
     2581 tgtgcctgca ctgcccccag gggcagtcgg ggatgggccc cccagagtgg atttccctcg
     2641 atctcgactc cgcttcaagg agaagcttgg cgagggccag tttggggagg tgcacctgtg
     2701 tgaggtcgac agccctcaag atctggttag tcttgatttc ccccttaatg tgcgtaaggg
     2761 acaccctttg ctggtagctg tcaagatctt acggccagat gccaccaaga atgccaggaa
     2821 tgatttcctg aaagaggtga agatcatgtc gaggctcaag gacccaaaca tcattcggct
     2881 gctgggcgtg tgtgtgcagg acgaccccct ctgcatgatt actgactaca tggagaacgg
     2941 cgacctcaac cagttcctca gtgcccacca gctggaggac aaggcagccg agggggcccc
     3001 tggggacggg caggctgcgc aggggcccac catcagctac ccaatgctgc tgcatgtggc
     3061 agcccagatc gcctccggca tgcgctatct ggccacactc aactttgtac atcgggacct
     3121 ggccacgcgg aactgcctag ttggggaaaa tttcaccatc aaaatcgcag actttggcat
     3181 gagccggaac ctctatgctg gggactatta ccgtgtgcag ggccgggcag tgctgcccat
     3241 ccgctggatg gcctgggagt gcatcctcat ggggaagttc acgactgcga gtgacgtgtg
     3301 ggcctttggt gtgaccctgt gggaggtgct gatgctctgt agggcccagc cctttgggca
     3361 gctcaccgac gagcaggtca tcgagaacgc gggggagttc ttccgggacc agggccggca
     3421 ggtgtacctg tcccggccgc ctgcctgccc gcagggccta tatgagctga tgcttcggtg
     3481 ctggagccgg gagtctgagc agcgaccacc cttttcccag ctgcatcggt tcctggcaga
     3541 ggatgcactc aacacggtga aaaagggtca agacaattct gcagatatcc agcacagtgg
     3601 cggccgctcg agtctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct
     3661 cggtctcgat tctacgcgta ccggtcatca tcaccatcac cattgagttt aaacccgctg
     3721 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     3781 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     3841 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     3901 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     3961 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     4021 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     4081 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     4141 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     4201 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     4261 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     4321 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     4381 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     4441 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     4501 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     4561 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     4621 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     4681 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     4741 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     4801 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     4861 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     4921 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     4981 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     5041 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     5101 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     5161 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     5221 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     5281 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     5341 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
     5401 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     5461 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     5521 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     5581 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     5641 ctctggggtt cgcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
     5701 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     5761 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     5821 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     5881 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     5941 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     6001 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     6061 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     6121 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     6181 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     6241 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     6301 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     6361 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     6421 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     6481 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     6541 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     6601 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     6661 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     6721 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     6781 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     6841 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     6901 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     6961 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     7021 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     7081 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     7141 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     7201 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     7261 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     7321 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     7381 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     7441 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     7501 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     7561 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     7621 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     7681 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     7741 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     7801 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     7861 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     7921 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     7981 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     8041 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     8101 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggat
//
