LOCUS       sequence-394500-        9542 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of human ERBB3 receptor tyrosine kinase in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9542)
  AUTHORS   Gudernova I, Foldynova-Trantirkova S, Ghannamova BE, Fafilek B, 
            Varecha M, Balek L, Hruba E, Jonatova L, Jelinkova I, Kunova 
            Bosakova M, Trantirek L, Mayer J, Krejci P
  TITLE     One reporter for in-cell activity profiling of majority of protein 
            kinase oncogenes.
  JOURNAL   Elife. 2017 Feb 15;6. pii: e21536. doi: 10.7554/eLife.21536.
  PUBMED    28199182
REFERENCE   2  (bases 1 to 9542)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-02-15"; volume: "6. pii: e21536. doi"; pages: " 
            10.7554/eLife.21536"
COMMENT     Sequence Label: pcDNA3.1-ERBB3-V5/HIS
FEATURES             Location/Qualifiers
     source          1..9542
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(43..62)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        234..613
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        614..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     768..788
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     862..881
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        862..880
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5039..5080
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5090..5107
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5130..5147)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5136..5360
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5406..5834
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5493..5512)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5703..5724
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5843..5863)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5848..6177
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6028..6163
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6090..6109
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6244..7038
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6298..6317)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6908..6927
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7214..7335
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7251..7270)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7305..7324
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7384..7400)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7384..7400)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7397..7419)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7408..7424
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7432..7438,7439..7456,7457..7462))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7477..7498
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7615..7632)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7786..8374)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7866..7885)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8545..9336,9337..9405))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9168..9187
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9406..9510)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
       61 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
      121 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
      181 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
      241 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      301 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      361 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      421 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
      481 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      541 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      601 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      661 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      721 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      781 taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac
      841 tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag ctggctagtt
      901 aagcttggta ccgagctcgg atccagtacc cttcaccatg agggcgaacg acgctctgca
      961 ggtgctgggc ttgcttttca gcctggcccg gggctccgag gtgggcaact ctcaggcagt
     1021 gtgtcctggg actctgaatg gcctgagtgt gaccggcgat gctgagaacc aataccagac
     1081 actgtacaag ctctacgaga ggtgtgaggt ggtgatgggg aaccttgaga ttgtgctcac
     1141 gggacacaat gccgacctct ccttcctgca gtggattcga gaagtgacag gctatgtcct
     1201 cgtggccatg aatgaattct ctactctacc attgcccaac ctccgcgtgg tgcgagggac
     1261 ccaggtctac gatgggaagt ttgccatctt cgtcatgttg aactataaca ccaactccag
     1321 ccacgctctg cgccagctcc gcttgactca gctcaccgag attctgtcag ggggtgttta
     1381 tattgagaag aacgataagc tttgtcacat ggacacaatt gactggaggg acatcgtgag
     1441 ggaccgagat gctgagatag tggtgaagga caatggcaga agctgtcccc cctgtcatga
     1501 ggtttgcaag gggcgatgct ggggtcctgg atcagaagac tgccagacat tgaccaagac
     1561 catctgtgct cctcagtgta atggtcactg ctttgggccc aaccccaacc agtgctgcca
     1621 tgatgagtgt gccgggggct gctcaggccc tcaggacaca gactgctttg cctgccggca
     1681 cttcaatgac agtggagcct gtgtacctcg ctgtccacag cctcttgtct acaacaagct
     1741 aactttccag ctggaaccca atccccacac caagtatcag tatggaggag tttgtgtagc
     1801 cagctgtccc cataactttg tggtggatca aacatcctgt gtcagggcct gtcctcctga
     1861 caagatggaa gtagataaaa atgggctcaa gatgtgtgag ccttgtgggg gactatgtcc
     1921 caaagcctgt gagggaacag gctctgggag ccgcttccag actgtggact cgagcaacat
     1981 tgatggattt gtgaactgca ccaagatcct gggcaacctg gactttctga tcaccggcct
     2041 caatggagac ccctggcaca agatccctgc cctggaccca gagaagctca atgtcttccg
     2101 gacagtacgg gagatcacag gttacctgaa catccagtcc tggccgcccc acatgcacaa
     2161 cttcagtgtt ttttccaatt tgacaaccat tggaggcaga agcctctaca accggggctt
     2221 ctcattgttg atcatgaaga acttgaatgt cacatctctg ggcttccgat ccctgaagga
     2281 aattagtgct gggcgtatct atataagtgc caataggcag ctctgctacc accactcttt
     2341 gaactggacc aaggtgcttc gggggcctac ggaagagcga ctagacatca agcataatcg
     2401 gccgcgcaga gactgcgtgg cagagggcaa agtgtgtgac ccactgtgct cctctggggg
     2461 atgctggggc ccaggccctg gtcagtgctt gtcctgtcga aattatagcc gaggaggtgt
     2521 ctgtgtgacc cactgcaact ttctgaatgg ggagcctcga gaatttgccc atgaggccga
     2581 atgcttctcc tgccacccgg aatgccaacc catggagggc actgccacat gcaatggctc
     2641 gggctctgat acttgtgctc aatgtgccca ttttcgagat gggccccact gtgtgagcag
     2701 ctgcccccat ggagtcctag gtgccaaggg cccaatctac aagtacccag atgttcagaa
     2761 tgaatgtcgg ccctgccatg agaactgcac ccaggggtgt aaaggaccag agcttcaaga
     2821 ctgtttagga caaacactgg tgctgatcgg caaaacccat ctgacaatgg ctttgacagt
     2881 gatagcagga ttggtagtga ttttcatgat gctgggcggc acttttctct actggcgtgg
     2941 gcgccggatt cagaataaaa gggctatgag gcgatacttg gaacggggtg agagcataga
     3001 gcctctggac cccagtgaga aggctaacaa agtcttggcc agaatcttca aagagacaga
     3061 gctaaggaag cttaaagtgc ttggctcggg tgtctttgga actgtgcaca aaggagtgtg
     3121 gatccctgag ggtgaatcaa tcaagattcc agtctgcatt aaagtcattg aggacaagag
     3181 tggacggcag agttttcaag ctgtgacaga tcatatgctg gccattggca gcctggacca
     3241 tgcccacatt gtaaggctgc tgggactatg cccagggtca tctctgcagc ttgtcactca
     3301 atatttgcct ctgggttctc tgctggatca tgtgagacaa caccgggggg cactggggcc
     3361 acagctgctg ctcaactggg gagtacaaat tgccaaggga atgtactacc ttgaggaaca
     3421 tggtatggtg catagaaacc tggctgcccg aaacgtgcta ctcaagtcac ccagtcaggt
     3481 tcaggtggca gattttggtg tggctgacct gctgcctcct gatgataagc agctgctata
     3541 cagtgaggcc aagactccaa ttaagtggat ggcccttgag agtatccact ttgggaaata
     3601 cacacaccag agtgatgtct ggagctatgg tgtgacagtt tgggagttga tgaccttcgg
     3661 ggcagagccc tatgcagggc tacgattggc tgaagtacca gacctgctag agaaggggga
     3721 gcggttggca cagccccaga tctgcacaat tgatgtctac atggtgatgg tcaagtgttg
     3781 gatgattgat gagaacattc gcccaacctt taaagaacta gccaatgagt tcaccaggat
     3841 ggcccgagac ccaccacggt atctggtcat aaagagagag agtgggcctg gaatagcccc
     3901 tgggccagag ccccatggtc tgacaaacaa gaagctagag gaagtagagc tggagccaga
     3961 actagaccta gacctagact tggaagcaga ggaggacaac ctggcaacca ccacactggg
     4021 ctccgccctc agcctaccag ttggaacact taatcggcca cgtgggagcc agagcctttt
     4081 aagtccatca tctggataca tgcccatgaa ccagggtaat cttggggagt cttgccagga
     4141 gtctgcagtt tctgggagca gtgaacggtg cccccgtcca gtctctctac acccaatgcc
     4201 acggggatgc ctggcatcag agtcatcaga ggggcatgta acaggctctg aggctgagct
     4261 ccaggagaaa gtgtcaatgt gtagaagccg gagcaggagc cggagcccac ggccacgcgg
     4321 agatagcgcc taccattccc agcgccacag tctgctgact cctgttaccc cactctcccc
     4381 acccgggtta gaggaagagg atgtcaacgg ttatgtcatg ccagatacac acctcaaagg
     4441 tactccctcc tcccgggaag gcaccctttc ttcagtgggt ctcagttctg tcctgggtac
     4501 tgaagaagaa gatgaagatg aggagtatga atacatgaac cggaggagaa ggcacagtcc
     4561 acctcatccc cctaggccaa gttcccttga ggagctgggt tatgagtaca tggatgtggg
     4621 gtcagacctc agtgcctctc tgggcagcac acagagttgc ccactccacc ctgtacccat
     4681 catgcccact gcaggcacaa ctccagatga agactatgaa tatatgaatc ggcaacgaga
     4741 tggaggtggt cctgggggtg attatgcagc catgggggcc tgcccagcat ctgagcaagg
     4801 gtatgaagag atgagagctt ttcaggggcc tggacatcag gccccccatg tccattatgc
     4861 ccgcctaaaa actctacgta gcttagaggc tacagactct gcctttgata accctgatta
     4921 ctggcatagc aggcttttcc ccaaggctaa tgcccagaga acgaaaaagg gtcaagacaa
     4981 ttctgcagat atccagcaca gtggcggccg ctcgagtcta gagggcccgc ggttcgaagg
     5041 taagcctatc cctaaccctc tcctcggtct cgattctacg cgtaccggtc atcatcacca
     5101 tcaccattga gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     5161 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     5221 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     5281 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     5341 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     5401 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     5461 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     5521 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     5581 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     5641 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     5701 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     5761 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     5821 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     5881 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     5941 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     6001 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     6061 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     6121 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     6181 ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt
     6241 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     6301 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     6361 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     6421 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     6481 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     6541 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     6601 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     6661 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     6721 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
     6781 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     6841 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     6901 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     6961 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     7021 cttgacgagt tcttctgagc gggactctgg ggttcgcgaa atgaccgacc aagcgacgcc
     7081 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     7141 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     7201 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     7261 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     7321 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     7381 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     7441 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     7501 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     7561 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     7621 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     7681 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     7741 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     7801 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     7861 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     7921 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     7981 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     8041 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     8101 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     8161 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     8221 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     8281 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     8341 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     8401 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     8461 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     8521 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     8581 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     8641 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     8701 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     8761 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     8821 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     8881 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     8941 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     9001 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     9061 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     9121 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     9181 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     9241 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     9301 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     9361 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     9421 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     9481 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     9541 cg
//
