LOCUS       sequence-394504-       10521 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Cas7-11 and Csx29 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10521)
  AUTHORS   Strecker J, Demircioglu FE, Li D, Faure G, Wilkinson ME, Gootenberg 
            JS, Abudayyeh OO, Nishimasu H, Macrae RK, Zhang F
  TITLE     RNA-activated protein cleavage with a CRISPR-associated 
            endopeptidase.
  JOURNAL   Science. 2022 Nov 25;378(6622):874-881. doi: 
            10.1126/science.add7450. Epub 2022 Nov 3.
  PUBMED    36423276
REFERENCE   2  (bases 1 to 10521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.add7450"; journalName: "Science"; date: 
            "2022-11-25- 25"; volume: "378"; issue: "6622"; pages: "874-881"
COMMENT     Sequence Label: pCDNA3_Cas7-11_T2A_Csx29
FEATURES             Location/Qualifiers
     source          1..10521
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(48..67)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        239..618
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..822
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     773..793
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     864..883
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        864..882
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5708..5761
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      6290..6299
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8023..8040
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8063..8080)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8069..8293
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8364..8380)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8364..8380)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8377..8399)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8388..8404
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8412..8418,8419..8436,8437..8442))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8457..8478
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8595..8612)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8766..9354)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8846..8865)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9525..10316,10317..10385))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10148..10167
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10386..10490)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatcgatc tcccgatccc ctagggtcta ctctcagtac aatctgctct
       61 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
      121 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
      181 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga
      241 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
      301 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
      361 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
      421 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
      481 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
      541 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
      601 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
      661 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
      721 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
      781 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag
      841 atccgctaga gatccgcggc cgctaatacg actcactata gggagagccg ccaccatgac
      901 gactactatg aagatttcaa ttgaattcct cgagccgttt cggatgacca aatggcagga
      961 aagcactagg cgaaacaaga acaacaagga atttgttcgc ggccaagctt ttgcaagatg
     1021 gcatagaaat aagaaggata acacaaaagg gcggccttac ataactggga cattgctgcg
     1081 gtccgcagta attagaagcg cggaaaacct gctgacattg agtgatggga aaattagcga
     1141 aaagacatgt tgtcctggga aatttgatac ggaagataaa gacaggttgc tgcaattgag
     1201 gcagcggtcc acccttcgtt ggaccgataa gaacccctgt ccagataacg ctgaaactta
     1261 ctgcccgttt tgcgaacttc tcgggagatc cggtaacgat ggaaagaagg ctgagaagaa
     1321 ggattggcgc tttcgtattc attttggcaa tctttccctt ccagggaagc ccgatttcga
     1381 cgggccaaaa gctataggca gccaacgggt acttaacagg gtcgatttca aatccggtaa
     1441 ggcccatgac ttctttaagg cttacgaagt cgaccatact aggtttcccc gcttcgaagg
     1501 ggagattacc atagataata aagtcagcgc tgaagccagg aaactgctct gtgattctct
     1561 caagtttacg gatcggctgt gtggagctct gtgcgtaatc aggttcgatg aatatacacc
     1621 agcagccgat agtgggaaac aaaccgaaaa tgtccaagca gaaccgaatg ctaatctcgc
     1681 tgaaaagacc gcagagcaaa ttattagcat tctggacgat aataagaaaa ccgaatatac
     1741 ccgcctgctt gctgatgcta ttcgttcact ccgccgctct agtaaacttg tcgctggctt
     1801 gccaaaggat catgacggga aggatgacca ttacctctgg gatatcggca agaagaagaa
     1861 agacgaaaac tctgtcacta ttaggcaaat ccttacgacc tcagcagaca ccaaggaact
     1921 caagaatgcc ggaaaatgga gagaattctg cgagaagctg ggtgaagcgc tgtacctcaa
     1981 gagtaaagac atgagtggcg gcctgaaaat tactcggaga atactgggcg atgctgaatt
     2041 ccatggaaag cccgatcggc ttgaaaagag ccggtctgtg tcaatcggat ctgtgttgaa
     2101 agaaactgtg gtatgcggcg aactggtcgc taaaacgccc ttcttcttcg gagcgataga
     2161 cgaagatgca aaacaaaccg acctgcaagt actcctcact cccgataaca agtatagact
     2221 gccaagaagc gccgtgcgag gtatactccg tcgagatctt caaacctatt ttgatagccc
     2281 atgcaatgct gagttgggtg gacggccatg catgtgtaag acgtgtagga ttatgagagg
     2341 gatcacggtg atggatgcgc gcagtgagta caatgccccg ccagaaataa ggcatcgtac
     2401 ccgcattaat cccttcacag gcacggttgc ggaaggtgcc ctgtttaata tggaagtagc
     2461 ccccgagggg attgtctttc catttcaact ccggtaccgg ggctctgaag atgggctgcc
     2521 cgatgcactg aaaacggtgt tgaaatggtg ggctgagggg caggcattca tgagtggcgc
     2581 tgcctcaacc gggaagggcc gattccggat ggaaaatgct aaatatgaaa cgctggatct
     2641 gagcgacgag aatcaaagga atgactatct taagaattgg ggatggcgtg acgagaaggg
     2701 gctcgaggaa ctgaagaaac gactgaactc aggtctgcca gagcccggta attataggga
     2761 tccaaaatgg cacgagatta acgtttccat tgagatggca agccctttta ttaatggcga
     2821 cccaatccgc gcagccgtgg acaaacgtgg tacagatgtg gttaccttcg ttaagtataa
     2881 agctgaaggg gaagaggcga aacccgtatg tgcatacaag gccgaatctt ttagaggggt
     2941 gatcagaagt gccgtggcac gcattcatat ggaagatggc gtccctttga ctgagttgac
     3001 tcacagtgac tgtgaatgtc tcctgtgcca aatctttgga agtgagtatg aagccggcaa
     3061 aataaggttt gaagatctcg tattcgaaag tgacccggaa cctgtgacct tcgatcatgt
     3121 ggccatcgat agattcactg gcggtgcagc tgataagaag aaattcgatg attcccctct
     3181 gcccggtagc cctgcaagac cgctcatgtt gaaaggctcc ttctggatcc gcagggacgt
     3241 tctcgaggac gaagagtact gtaaggcact cggtaaggct cttgcagatg tgaataatgg
     3301 cctttatccc ctcggtggaa agagcgccat cggctacgga caggtcaaga gtctgggtat
     3361 aaagggagat gataagagga tttctcgcct catgaatcct gcctttgatg agacagatgt
     3421 agccgttcca gaaaagccca aaactgatgc cgaggttcgc atcgaggcag agaaagtata
     3481 ttacccacac tatttcgtcg aaccccataa gaaggtggaa cgcgaggaga aaccctgtgg
     3541 tcatcaaaag ttccacgagg ggcgactgac aggtaaaatt cggtgtaagc tcattaccaa
     3601 gacacccctc atcgtcccag atactagtaa tgacgatttc ttcagacctg cggataaaga
     3661 agctcggaag gaaaaggacg aatatcataa atcatatgct ttcttcagac ttcataaaca
     3721 aatcatgatt cccgggagcg aattgagagg aatggtgagt agtgtctacg aaactgtgac
     3781 aaattcttgc ttcaggatat ttgatgagac taaacggttg tcatggcgga tggatgctga
     3841 tcaccagaat gtgctgcaag actttctccc aggtcgagtg accgccgatg ggaaacatat
     3901 acaaaagttt tccgaaactg caagggtgcc tttctatgac aaaacgcaga aacattttga
     3961 cattcttgat gagcaagaaa ttgctggtga gaaacctgtt cggatgtggg tcaagcgttt
     4021 tattaaacga ctgagcctcg ttgatcctgc taaacacccc cagaagaaac aagacaataa
     4081 atggaaaaga cgcaaagaag gcatcgccac atttatagag caaaagaatg gctcttatta
     4141 ttttaacgtg gtcaccaata atggctgcac ttctttccac ttgtggcata aacctgacaa
     4201 ttttgaccag gagaaactcg aaggcataca gaatggtgag aaactggatt gctgggtaag
     4261 agatagtaga taccaaaagg cctttcaaga gatacccgag aacgacccag acggatggga
     4321 gtgtaaagag ggctaccttc atgtcgtcgg ccccagcaaa gtagagttta gtgacaagaa
     4381 aggggatgtg atcaataact ttcaaggaac actcccatca gttcccaacg actggaaaac
     4441 aattaggacg aacgatttta agaataggaa aaggaagaat gaacctgtgt tttgttgtga
     4501 ggatgataag ggcaattact ataccatggc taaatattgc gaaaccttct tcttcgatct
     4561 gaaggagaac gaggaatacg agatccccga gaaagcaaga atcaaataca aagaactgct
     4621 cagggtctat aacaacaatc ctcaagcagt gccggagagc gtatttcagt ctagagttgc
     4681 ccgggaaaac gtggaaaagc tgaagtccgg agatcttgtg tatttcaaac ataatgaaaa
     4741 gtacgtagag gacatcgtcc cagtgcggat ttcccgaact gtagacgata ggatgatcgg
     4801 caaacgtatg agcgccgatc tgcggccgtg ccatggagat tgggtggaag atggtgatct
     4861 cagtgccttg aatgcatatc ccgagaaaag actcctcttg cgccacccca aaggactctg
     4921 ccctgcttgc cggctctttg gaaccggatc ttacaagggc agagtcaggt ttggattcgc
     4981 gtcactcgaa aacgatccgg agtggctgat cccaggcaag aatcccggcg atccgtttca
     5041 cggcgggccg gtgatgctct cattgttgga acggcctcgc ccgacttgga gtataccggg
     5101 atccgacaat aagtttaaag tgcctggcag aaagttttac gtccaccacc acgcctggaa
     5161 aaccattaag gacgggaacc atcccacaac aggcaaagct attgaacaaa gccctaataa
     5221 ccgcactgta gaagctctcg ccggcgggaa ttcctttagc ttcgaaattg cctttgagaa
     5281 cctgaaagaa tgggagctgg gtttgctcat ccacagcctg caactcgaaa agggtctggc
     5341 gcataaactt ggaatggcaa agtctatggg atttggttca gttgaaattg acgtcgaatc
     5401 agtgcgcctg agaaaagatt ggaagcaatg gcggaatggc aattccgaaa ttcccaactg
     5461 gttgggaaaa ggatttgcta aactgaagga atggttccgg gacgagctcg attttataga
     5521 aaatcttaag aaacttcttt ggtttcctga gggcgaccaa gcaccccggg tttgctaccc
     5581 catgctgcga aagaaggacg atcctaatgg gaatagcggt tacgaagaac tcaaagacgg
     5641 ggaattcaag aaagaagatc ggcagaagaa gctgaccacg ccgtggacac cgtgggcagg
     5701 cagcggcgag ggcagaggct ccctgctgac ctgcggcgac gtggaagaga accccgggcc
     5761 catgagcaat cctatcagag atatccagga cagactgaag accgccaagt tcgacaacaa
     5821 ggacgacatg atgaacctgg cttcaagcct gtacaagtac gagaaacagc tgatggacag
     5881 ctctgaggct acactgtgcc agcagggcct gagcaaccgg cctaactctt tttctcagct
     5941 gagccaattt cgggacagcg acattcagag caaggccggc ggccagaccg gtaagttctg
     6001 gcagaacgag tacgaggctt gtaaaaactt ccagacacac aaggagagaa gagaaaccct
     6061 ggaacagatc atcagattcc tgcaaaatgg cgctgaggaa aaggacgctg atgatctgct
     6121 gctgaagacc ctggcccgag cctacttcca ccgtggactg ctgtatagac ctaagggctt
     6181 cagcgtgcct gcgaggaagg tggaagccat gaaaaaggcc atcgcctact gcgaaatcat
     6241 ccttgataag aatgaggaag aatcggaggc tctgagaatc tggctgtacg ccgccatgga
     6301 actccggaga tgcggcgagg agtatcctga gaactttgcg gagaagctgt tctaccttgc
     6361 caacgacggc ttcatcagcg agctgtacga catccggctg ttcctggaat acaccgagag
     6421 agaagaggac aacaacttcc tggacatgat cctgcaggag aatcaggaca gagaaagact
     6481 gttcgagctg tgtctgtaca aggccagagc ctgttttcac ctgaaccagc tgaacgacgt
     6541 gcggatctac ggcgagagcg caatcgacaa cgcccctggc gccttcgccg atcctttctg
     6601 ggatgaactg gtggagttca tcagaatgct gcggaacaag aagtctgaac tgtggaagga
     6661 aattgccatc aaggcctggg acaagtgcag agaaaaggaa atgaaggtgg gcaacaacat
     6721 ctacctttct tggtactggg ccagacagag agagctgtac gacctggcct tcatggccca
     6781 agacggcatc gagaagaaaa caagaatcgc cgatagcctg aagagccgga ccaccctgag
     6841 aatccaggag ctgaatgagc tgagaaagga tgcccaccgg aagcagaacc gtcggctgga
     6901 agataagctg gatcggatca tcgagcagga gaacgaggcc agagacggcg cctacctgcg
     6961 gagaaacccc ccctgcttca ccggcggaaa aagagaggaa atcccctttg ccagacttcc
     7021 acagaactgg atcgccgtgc acttctatct gaacgagctg gaaagccacg aaggcggcaa
     7081 gggcggccac gccctgatct acgaccctca gaaggctgag aaagatcagt ggcaagacaa
     7141 gagcttcgac tacaaagaac tgcacagaaa attcctcgag tggcaggaga actacatcct
     7201 gaacgaggag ggcagcgccg atttcctggt gacactgtgc agagagatcg agaaagccat
     7261 gcctttcctg tttaagtccg aggtgatccc cgaggacaga cccgtgctgt ggattcctca
     7321 cggcttcctg catagactgc ctctgcacgc cgctatgaag agcggaaaca attctaatat
     7381 cgagatcttc tgggagcggc acgccagcag atatctccct gcttggcacc tgttcgatcc
     7441 tgccccatac agccgcgagg aaagctccac cctgctgaag aacttcgagg agtacgactt
     7501 ccagaacctg gaaaacggcg aaatcgaggt ctacgccccc agcagcccca agaaggtgaa
     7561 ggaagccatc cgggaaaacc ccgctattct gctgctgctg tgtcacggcg aggccgacat
     7621 gacaaaccct ttccggagct gcctgaagct gaaaaacaaa gatatgacca tcttcgacct
     7681 gctgaccgtg gaagatgtga ggctgtctgg cagcagaatc cttctgggag cctgcgagtc
     7741 tgacatggtg ccacctctgg agttcagcgt ggacgaacac ctgagcgtgt ccggcgcttt
     7801 tctcagccat aaggccggag aaatcgtcgc cggcctgtgg accgtggact ctgagaaggt
     7861 tgacgagtgc tacagctacc tggtggaaga gaaggacttc ctgagaaacc tgcaggagtg
     7921 gcagatggcc gagacagaga acttcagaag cgagaatgat agctccctgt tctacaagat
     7981 cgccccattt cggatcattg gctttcctgc cgaatgaccg gtcatcatca ccatcaccat
     8041 tgagtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     8101 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     8161 taaaatgaga aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     8221 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     8281 gtgggctcta tggcttctga ggcggaaaga accagctggg gctcgatacc gtcgacctct
     8341 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     8401 acaattccac acaacatacg agccggaagc ataaagtgta aagcctaggg tgcctaatga
     8461 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     8521 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     8581 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     8641 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     8701 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     8761 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     8821 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     8881 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     8941 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     9001 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     9061 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     9121 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     9181 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     9241 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     9301 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     9361 cctttgatct tttctacggg gtctgacact cagtggaacg aaaactcacg ttaagggatt
     9421 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     9481 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     9541 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     9601 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     9661 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     9721 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     9781 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     9841 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     9901 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     9961 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    10021 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    10081 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    10141 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    10201 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    10261 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    10321 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    10381 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    10441 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    10501 cgaaaagtgc cacctgacgt c
//
