LOCUS       sequence-394508-        8274 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial protein expression of Edc3 283-551.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8274)
  AUTHORS   Currie SL, Xing W, Muhlrad D, Decker CJ, Parker R, Rosen MK
  TITLE     Quantitative reconstitution of yeast RNA processing bodies.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Apr 4;120(14):e2214064120. doi: 
            10.1073/pnas.2214064120. Epub 2023 Mar 27.
  PUBMED    36972455
REFERENCE   2  (bases 1 to 8274)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-04-4"; volume: "120(14):e2214064120. 
            doi"; pages: " 10.1073/pnas.2214064120. Epub 2023 Mar 27"
COMMENT     Sequence Label: Edc3 283-551-EGFP
FEATURES             Location/Qualifiers
     source          1..8274
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(90..95,96..111,112..118)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    126..142
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     131..153
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             212..1312
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      1169..1178
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1286..1309
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1361..1372
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1385..1405
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(2246..2248,2249..2251,2252..2962)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2291..2312)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2552..2571)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2899..2920
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2975..2995
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2996..3013
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3026..3043)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3026..3042)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3035..3057)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3221..3238)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3391..3477
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3569..3596
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3615..3706
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3707..3775,3776..4567)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3925..3944)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(4609..5122)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4804..4825)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5016..5035
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      5233..5821
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5722..5741
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5975..5992
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6098..6117)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6258..6280
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6274..6465)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        6961..7038
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             7039..8121
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(7058..7077)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg acatcataac
       61 ggttctggca aatattctga aatgagctgt tgacaattaa tcatcggctc gtataatgtg
      121 tggaattgtg agcggataac aatttcacac aggaaacagc cagtccgttt aggtgttttc
      181 acgagcactt caccaacaag gaccatagat tatgaaaact gaagaaggta aactggtaat
      241 ctggattaac ggcgataaag gctataacgg tctcgctgaa gtcggtaaga aattcgagaa
      301 agataccgga attaaagtca ccgttgagca tccggataaa ctggaagaga aattcccaca
      361 ggttgcggca actggcgatg gccctgacat tatcttctgg gcacacgacc gctttggtgg
      421 ctacgctcaa tctggcctgt tggctgaaat caccccggac aaagcgttcc aggacaagct
      481 gtatccgttt acctgggatg ccgtacgtta caacggcaag ctgattgctt acccgatcgc
      541 tgttgaagcg ttatcgctga tttataacaa agatctgctg ccgaacccgc caaaaacctg
      601 ggaagagatc ccggcgctgg ataaagaact gaaagcgaaa ggtaagagcg cgctgatgtt
      661 caacctgcaa gaaccgtact tcacctggcc gctgattgct gctgacgggg gttatgcgtt
      721 caagtatgaa aacggcaagt acgacattaa agacgtgggc gtggataacg ctggcgcgaa
      781 agcgggtctg accttcctgg ttgacctgat taaaaacaaa cacatgaatg cagacaccga
      841 ttactccatc gcagaagctg cctttaataa aggcgaaaca gcgatgacca tcaacggccc
      901 gtgggcatgg tccaacatcg acaccagcaa agtgaattat ggtgtaacgg tactgccgac
      961 cttcaagggt caaccatcca aaccgttcgt tggcgtgctg agcgcaggta ttaacgccgc
     1021 cagtccgaac aaagagctgg caaaagagtt cctcgaaaac tatctgctga ctgatgaagg
     1081 tctggaagcg gttaataaag acaaaccgct gggtgccgta gcgctgaagt cttacgagga
     1141 agagttggcg aaagatccac gtattgccgc caccatggaa aacgcccaga aaggtgaaat
     1201 catgccgaac atcccgcaga tgtccgcttt ctggtatgcc gtgcgtactg cggtgatcaa
     1261 cgccgccagc ggtcgtcaga ctgtcgatga agccctgaaa gacgcgcaga ctaattcgag
     1321 ctcgaacaac aacaacaata acaataacaa caacctcggg atcgagggaa ggatttcaga
     1381 atttgaaaac ctgtattttc agggccatat gcctatggct acaccagtac aactactaga
     1441 aatggagagc atcacgtccg aatttttcag tattaactcg gcagggctac tagaaaattt
     1501 tgctgtaaac gcatcgttct tcttaaagca gaaactaggt ggccgtgcac gtttacgttt
     1561 acaaaattct aatccggaac ctttagtagt aatactagcc tcagattcca acagatctgg
     1621 tgcgaaagct ctggcgttgg gtagacatct ttgccaaacg gggcatatcc gtgtcataac
     1681 attatttaca tgttctcaaa atgaactaca ggattccatg gtcaaaaagc aaacagatat
     1741 ttacaagaag tgtggcggaa aaattgttaa tagtgtatcg tcgctggaat ctgctatgga
     1801 aacattaaat agccctgtag aaatagtcat cgatgccatg cagggatatg actgtacatt
     1861 gagcgatctg gcggggacgt cggaagttat tgaaagcaga attaaaagca tgatatcatg
     1921 gtgtaacaaa cagcgaggat ctactaaagt gtggtctttg gatattccaa atgggtttga
     1981 tgcgggctcg ggcatgccag atattttctt ttcagacagg attgaagcaa caggaattat
     2041 ttgttctggc tggcctttga ttgccatcaa caacttaatt gcaaatttgc caagtctaga
     2101 agatgctgtt ttgattgata taggtatacc acagggcgcc tattcacaga gaacttcttt
     2161 gcgtaagttc caaaactgtg atcttttcgt cactgacggg tccctgctat tagatttggg
     2221 atccgggtct ggttcaggaa tggccatggt gagcaagggc gaggagctgt tcaccggggt
     2281 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     2341 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     2401 caagctgccc gtgccctggc ccaccctcgt gaccaccttg acctacggcg tgcagtgctt
     2461 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     2521 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     2581 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     2641 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaaggtcta
     2701 tatcaccgcc gacaagcaga agaacggcat caaggtgaac ttcaagaccc gccacaacat
     2761 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     2821 ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga gcaaagaccc
     2881 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     2941 cggcatggac gagctgtaca agggaagcgg ccgcgaaaac ctgtattttc agggccatca
     3001 tcatcatcat cactgaaagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     3061 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     3121 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     3181 ggcagcttgg ctgttttggc ggatgagata agattttcag cctgatacag attaaatcag
     3241 aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac
     3301 ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc
     3361 cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac
     3421 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg
     3481 ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg
     3541 ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg
     3601 tttctacaaa ctcttttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     3661 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     3721 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     3781 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     3841 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgtttccc
     3901 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg
     3961 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     4021 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     4081 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     4141 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     4201 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     4261 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     4321 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     4381 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     4441 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     4501 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     4561 ttggtaactg tcagaccaag tttactcata tatactttag attgatttac cccggttgat
     4621 aatcagaaaa gccccaaaaa caggaagatt gtataagcaa atatttaaat tgtaaacgtt
     4681 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     4741 gccgaaatcg gcaaaatccc ttataaatca aaagaatagc ccgagatagg gttgagtgtt
     4801 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     4861 aaaaccgtct atcagggcga tggcccacta cgtgaaccat cacccaaatc aagttttttg
     4921 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     4981 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     5041 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     5101 aatgcgccgc tacagggcgc gtaaaaggat ctaggtgaag atcctttttg ataatctcat
     5161 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     5221 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     5281 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     5341 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     5401 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     5461 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     5521 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     5581 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     5641 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     5701 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     5761 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     5821 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     5881 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     5941 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     6001 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatgtg
     6061 gtgcactctc agtacaccgc atgtggtgca ctctcagtac aatctgctct gatgccgcat
     6121 agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca
     6181 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     6241 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     6301 acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgcagcgatt cacagatgtc
     6361 tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct
     6421 gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta
     6481 agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat
     6541 acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc
     6601 ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa
     6661 tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat
     6721 aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac
     6781 cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc
     6841 gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct
     6901 caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgaaattcc
     6961 gacaccatcg aatggtgcaa aacctttcgc ggtatggcat gatagcgccc ggaagagagt
     7021 caattcaggg tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt
     7081 gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg
     7141 cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa
     7201 caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac
     7261 gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg
     7321 gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt
     7381 ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt
     7441 gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca
     7501 cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg
     7561 gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg
     7621 cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg
     7681 gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat
     7741 gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg
     7801 cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac
     7861 gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc
     7921 ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag
     7981 ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg
     8041 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     8101 cgactggaaa gcgggcagtg agcgcaacgc aattaatgta agttagctca ctcattaggc
     8161 acaattctca tgtttgacag cttatcatcg actgcacggt gcaccaatgc ttctggcgtc
     8221 aggcagccat cggaagctgt ggtatggctg tgcaggtcgt aaatcactgc ataa
//
