LOCUS       sequence-394512-        9837 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial protein expression of Pat1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9837)
  AUTHORS   Currie SL, Xing W, Muhlrad D, Decker CJ, Parker R, Rosen MK
  TITLE     Quantitative reconstitution of yeast RNA processing bodies.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Apr 4;120(14):e2214064120. doi: 
            10.1073/pnas.2214064120. Epub 2023 Mar 27.
  PUBMED    36972455
REFERENCE   2  (bases 1 to 9837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-04-4"; volume: "120(14):e2214064120. 
            doi"; pages: " 10.1073/pnas.2214064120. Epub 2023 Mar 27"
COMMENT     Sequence Label: Pat1-mCh
FEATURES             Location/Qualifiers
     source          1..9837
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(107..112,113..128,129..135)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    143..159
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     148..170
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             229..1329
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      1186..1195
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1303..1326
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1378..1389
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1402..1422
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3835..4542
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3980..3998)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4248..4267
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4442..4465
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             4555..4575
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4576..4593
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4606..4623)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4606..4622)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4615..4637)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4801..4818)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      4971..5057
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5149..5176
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        5195..5286
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5287..5355,5356..6147)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5505..5524)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(6189..6702)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6384..6405)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6596..6615
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      6813..7401
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7302..7321
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7555..7572
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7678..7697)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7838..7860
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7854..8045)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        8541..8618
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             8619..9701
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(8638..8657)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 cgtaaatcac tgcataattc gtgtcgctca aggcgcactc ccgttctgga taatgttttt
       61 tgcgccgaca tcataacggt tctggcaaat attctgaaat gagctgttga caattaatca
      121 tcggctcgta taatgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagccag
      181 tccgtttagg tgttttcacg agcacttcac caacaaggac catagattat gaaaactgaa
      241 gaaggtaaac tggtaatctg gattaacggc gataaaggct ataacggtct cgctgaagtc
      301 ggtaagaaat tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg
      361 gaagagaaat tcccacaggt tgcggcaact ggcgatggcc ctgacattat cttctgggca
      421 cacgaccgct ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa
      481 gcgttccagg acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg
      541 attgcttacc cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tctgctgccg
      601 aacccgccaa aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt
      661 aagagcgcgc tgatgttcaa cctgcaagaa ccgtacttca cctggccgct gattgctgct
      721 gacgggggtt atgcgttcaa gtatgaaaac ggcaagtacg acattaaaga cgtgggcgtg
      781 gataacgctg gcgcgaaagc gggtctgacc ttcctggttg acctgattaa aaacaaacac
      841 atgaatgcag acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg
      901 atgaccatca acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt
      961 gtaacggtac tgccgacctt caagggtcaa ccatccaaac cgttcgttgg cgtgctgagc
     1021 gcaggtatta acgccgccag tccgaacaaa gagctggcaa aagagttcct cgaaaactat
     1081 ctgctgactg atgaaggtct ggaagcggtt aataaagaca aaccgctggg tgccgtagcg
     1141 ctgaagtctt acgaggaaga gttggcgaaa gatccacgta ttgccgccac catggaaaac
     1201 gcccagaaag gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg
     1261 cgtactgcgg tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc cctgaaagac
     1321 gcgcagacta attcgagctc gaacaacaac aacaataaca ataacaacaa cctcgggatc
     1381 gagggaagga tttcagaatt tgaaaacctg tattttcagg gccatatgtc cttctttggg
     1441 ttagaaaata gcggtaatgc gcgggatggt cctctggact ttgaagagag ttacaagggc
     1501 tatggcgagc acgaacttga ggagaacgac tatttgaacg acgaaacatt tggtgataat
     1561 gttcaggttg gtaccgactt tgattttgga aatcctcaca gcagcggcag cagcggcaac
     1621 gcaattggtg gtaatggcgt cggtgccacg gctagatcgt atgttgcagc tactgcagaa
     1681 ggaattagcg gccctaggac cgatggaacg gcagcagcag gacctctaga cctgaagcca
     1741 atggaatctt tgtggtctac tgcaccacct ccagcaatgg cgccttcacc ccaaagtaca
     1801 atggctccgg ctcctgctcc gcagcaaatg gcccccctac agccaatctt gtcgatgcaa
     1861 gacttggaaa gacaacaacg tcaaatgcag caacagttta tgaatttcca cgccatgggt
     1921 catccacagg gtctcccaca gggtccgcct cagcagcaat ttccaatgca gcctgcgtcg
     1981 ggtcaaccag gtccctcaca atttgcgcct ccacctccac ctcctggcgt taatgtgaat
     2041 atgaatcaaa tgccaatggg tcctgtacaa gttccagttc aagcttcgcc ttcacccatc
     2101 ggtatgtcca acactccttc tccaggccct gtggttggcg caactaaaat gcctctgcaa
     2161 agtggacgca gatcgaagag agatttgtcg cctgaagagc aaagacgttt gcagattcgt
     2221 catgccaaag tggagaaaat cttgaaatac tcaggtttaa tgactcctcg tgataaggac
     2281 ttcatcacca gatatcagtt gtctcaaatt gtcactgagg acccttacaa tgaggatttc
     2341 tacttccagg tctacaagat tatccaaaga ggcggtatca cgtccgaatc caacaaaggt
     2401 ttgattgcta gggcgtattt ggaacattct ggacacagac tcggtggtcg ctataagaga
     2461 accgatattg ccctacagag aatgcaaagt caagtagaaa aggctgtcac tgtggctaag
     2521 gaaagacctt ctaagttgaa ggatcaacaa gcggctgctg gtaactctag ccaggataat
     2581 aagcaagcaa acacggttct gggcaaaatc tcttccactt tgaacagcaa gaatccaaga
     2641 agacaactgc agatccccag acaacagcct tctgaccccg atgcgctaaa agacgtcact
     2701 gactctctga ccaacgtgga cttggcctct tcagggtcct cctctacggg ctcttctgcc
     2761 gctgctgttg cttctaagca aagaagaaga tcttcatacg cgttcaacaa cggtaatggt
     2821 gccacaaatt tgaacaaatc tgggggcaaa aaattcattc ttgagttaat tgaaacagtt
     2881 tatgaagaga ttttagactt ggaagctaac ttgaggaatg gccagcaaac tgacagcact
     2941 gcaatgtggg aggcccttca catcgacgac agttcgtatg acgtaaaccc tttcatttcg
     3001 atgctatcat ttgataaagg tatcaagatt atgcctagaa tttttaattt cttggataag
     3061 cagcaaaaat tgaaaatcct gcaaaaaatc ttcaatgaat tatcacactt gcaaatcatc
     3121 atattgagtt cctacaagac tacaccaaaa ccaactttga cacaattgaa gaaagtcgat
     3181 ctgttccaaa tgatcatatt aaagatcatt gtctcgtttt tgtctaataa ctccaatttt
     3241 atcgaaatta tgggtctgtt gctacagtta atcagaaaca acaacgtttc gttcttgacc
     3301 acctccaaaa ttggtctaaa tttgatcacc attttgattt ctcgtgccgc attaatcaag
     3361 caagattcat caagatctaa tattctttcc tctcctgaaa tctccacatg gaatgagatt
     3421 tatgataaat tattcacttc attggaaagt aagattcagc tgattttccc tccaagggaa
     3481 tataacgacc acatcatgcg tttacaaaat gacaagttta tggatgaagc atacatttgg
     3541 cagttcctag ctagtttagc actaagtgga aagctaaacc accagagaat cattattgat
     3601 gaagtacgtg atgaaatctt tgccactatt aacgaggcgg agaccttaca aaagaaagag
     3661 aaagaattga gtgtattacc tcagaggtct caagaattag acacagagtt aaaatctatt
     3721 atttataata aagagaaact ataccaagat ttgaatttgt tcctaaacgt tatggggttg
     3781 gtgtatcgcg atggtgaaat atcagaacta aagggatccg ggtctggttc aggaatggtg
     3841 agcaagggcg aggaggacaa catggccatc atcaaggagt tcatgcgctt caaggtgcac
     3901 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
     3961 tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc
     4021 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc
     4081 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     4141 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag
     4201 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
     4261 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg
     4321 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgccgaggtc
     4381 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc
     4441 aagctggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgcgcc
     4501 gagggccgcc actccaccgg cggcatggac gagctgtaca agggaagcgg ccgcgaaaac
     4561 ctgtattttc agggccatca tcatcatcat cactgaaagc ttggcactgg ccgtcgtttt
     4621 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     4681 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     4741 gcgcagcctg aatggcgaat ggcagcttgg ctgttttggc ggatgagata agattttcag
     4801 cctgatacag attaaatcag aacgcagaag cggtctgata aaacagaatt tgcctggcgg
     4861 cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac gccgtagcgc
     4921 cgatggtagt gtggggtctc cccatgcgag agtagggaac tgccaggcat caaataaaac
     4981 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     5041 tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag
     5101 ggtggcgggc aggacgcccg ccataaactg ccaggcatca aattaagcag aaggccatcc
     5161 tgacggatgg cctttttgcg tttctacaaa ctcttttgtt tatttttcta aatacattca
     5221 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     5281 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     5341 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     5401 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     5461 cgccccgaag aacgtttccc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     5521 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     5581 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     5641 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     5701 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     5761 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     5821 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     5881 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     5941 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     6001 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     6061 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     6121 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     6181 attgatttac cccggttgat aatcagaaaa gccccaaaaa caggaagatt gtataagcaa
     6241 atatttaaat tgtaaacgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     6301 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaatagc
     6361 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     6421 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     6481 cacccaaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     6541 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     6601 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     6661 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtaaaaggat ctaggtgaag
     6721 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     6781 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     6841 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     6901 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     6961 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     7021 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     7081 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     7141 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     7201 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     7261 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     7321 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     7381 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     7441 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     7501 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     7561 tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc
     7621 ggtatttcac accgcatgtg gtgcactctc agtacaccgc atgtggtgca ctctcagtac
     7681 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg
     7741 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     7801 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     7861 ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg
     7921 tgcagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga
     7981 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg
     8041 gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa
     8101 cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt
     8161 tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt
     8221 caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct
     8281 gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac
     8341 gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag
     8401 cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc
     8461 cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac
     8521 ccaacgctgc ccgaaattcc gacaccatcg aatggtgcaa aacctttcgc ggtatggcat
     8581 gatagcgccc ggaagagagt caattcaggg tggtgaatgt gaaaccagta acgttatacg
     8641 atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca
     8701 gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca
     8761 ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca
     8821 cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg
     8881 atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta
     8941 aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc
     9001 tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc
     9061 ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc
     9121 gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc
     9181 cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca
     9241 atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac
     9301 aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc
     9361 agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata
     9421 tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca
     9481 ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct
     9541 ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa
     9601 ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     9661 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgta
     9721 agttagctca ctcattaggc acaattctca tgtttgacag cttatcatcg actgcacggt
     9781 gcaccaatgc ttctggcgtc aggcagccat cggaagctgt ggtatggctg tgcaggt
//
