LOCUS       sequence-394513-       10982 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For protein purification of Cas7-11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10982)
  AUTHORS   Strecker J, Demircioglu FE, Li D, Faure G, Wilkinson ME, Gootenberg 
            JS, Abudayyeh OO, Nishimasu H, Macrae RK, Zhang F
  TITLE     RNA-activated protein cleavage with a CRISPR-associated 
            endopeptidase.
  JOURNAL   Science. 2022 Nov 25;378(6622):874-881. doi: 
            10.1126/science.add7450. Epub 2022 Nov 3.
  PUBMED    36423276
REFERENCE   2  (bases 1 to 10982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.add7450"; journalName: "Science"; date: 
            "2022-11-25- 25"; volume: "378"; issue: "6622"; pages: "874-881"
COMMENT     Sequence Label: SUMO_TwinStrep_Cas7-11_crRNA
FEATURES             Location/Qualifiers
     source          1..10982
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     118..137
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     371..388
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    403..542
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(468..487)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     628..650
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(644..835)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(2147..3229)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     3191..3210
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(3230..3307)
                     /gene="lacI"
                     /label=lacI promoter
     promoter        3408..3426
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      3473..3500
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     3606..3625
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        3709..3727
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3728..3752
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3767..3789
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3808..3825
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3835..3852
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             join(3865..3888,3889..3924,3925..3948)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             3949..4242
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
     primer_bind     complement(9098..9116)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      9112..9159
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(9334..9339,9340..9356,9357..9362))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(9389..9407)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9475..9579
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9580..9648,9649..10440)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9798..9817)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(10611..10982,1..217)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
       61 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
      121 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
      181 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
      241 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
      301 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
      361 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct
      421 ccttacgcat ctgtgcggta tttcacaccg caatggtgca ctctcagtac aatctgctct
      481 gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg
      541 cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
      601 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
      661 catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt
      721 cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg
      781 tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg
      841 cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga
      901 tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta
      961 aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc
     1021 gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga
     1081 tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga
     1141 aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc
     1201 acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta
     1261 gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc
     1321 ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag
     1381 ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg
     1441 tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc
     1501 accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa
     1561 cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccaatgat
     1621 cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc
     1681 tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag
     1741 aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag
     1801 cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg
     1861 accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc
     1921 gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg
     1981 cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat
     2041 gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga
     2101 tcccggtgcc taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt
     2161 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     2221 cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct
     2281 gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc
     2341 ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt
     2401 cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg gactcggtaa
     2461 tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga
     2521 tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt
     2581 cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac
     2641 gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca
     2701 atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt
     2761 tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt
     2821 ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt
     2881 gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg
     2941 acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg
     3001 acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg
     3061 ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt
     3121 tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat
     3181 aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc
     3241 tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga
     3301 tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt
     3361 agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgtaa tacgactcac
     3421 tataggttga tgtcacggaa cctttgttgt cttcgacatg ggtaatcctc atggctcacc
     3481 ttcgggtggg cctttctgcg caaggagatg gcgcccaaca gtcccccggc cacggggcct
     3541 gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc
     3601 ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg
     3661 gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaatta atacgactca
     3721 ctatagggga attgtgagcg gataacaatt cccctctaga aataattttg tttaacttta
     3781 agaaggagat ataccatggg cagcagccat catcatcatc atcacagcag cggcctggtg
     3841 ccgcgcggca gccatatggc tagctggagc catccgcagt ttgaaaaagg tggtggtagc
     3901 ggtggtggtt caggtggtag tgcatggtca caccctcagt ttgagaaaat gtcggactca
     3961 gaagtcaatc aagaagctaa gccagaggtc aagccagaag tcaagcctga gactcacatc
     4021 aatttaaagg tgtccgatgg atcttcagag atcttcttca agatcaaaaa gaccactcct
     4081 ttaagaaggc tgatggaagc gttcgctaaa agacagggta aggaaatgga ctccttaaga
     4141 ttcttgtacg acggtattag aatccaagct gatcagaccc ctgaagattt ggacatggag
     4201 gataacgata ttattgaggc tcacagagaa cagattggtg gatccatgac cactaccatg
     4261 aagatcagca tcgagttctt ggagcccttc cgcatgacga agtggcagga gtccacgcgc
     4321 cgcaataaga acaataaaga gtttgtgcgt ggccaagcgt ttgcccgttg gcaccgcaac
     4381 aagaaagata acaccaaggg tcgtccgtac attactggta cgctcctgcg ctcagcggtg
     4441 attcgctccg cggagaacct gctgacactg agtgacggta agatctccga gaaaacctgc
     4501 tgcccgggga aattcgacac ggaagataag gaccgtttac tccagctgcg tcaacgctcc
     4561 acgctgcgtt ggacagacaa gaatccgtgt cccgacaatg cggaaacgta ttgtccgttc
     4621 tgtgagctcc tcggccgctc cggaaatgac ggtaagaaag cagaaaagaa ggactggcgc
     4681 ttccgcatcc attttggtaa cctttccctg ccgggcaagc ccgacttcga cggtccgaag
     4741 gcaattggtt cacagcgcgt attaaatcgt gtggatttta agtcgggaaa ggcacatgac
     4801 ttcttcaagg cttatgaggt ggaccatacc cgcttccctc gctttgaagg ggagatcacc
     4861 atcgacaata aagtgtccgc cgaggcacgt aagttgctgt gtgacagcct taaattcacg
     4921 gatcgcctgt gtggagccct gtgcgtcatc cgcttcgacg agtatacacc ggccgcggac
     4981 agcggtaagc aaaccgagaa tgtgcaggca gagcccaacg caaacctggc ggagaaaact
     5041 gccgagcaaa ttatctcgat cctcgatgac aacaagaaaa cggaatacac ccgcctttta
     5101 gcggacgcca tccgctcatt gcgtcgctcc tcaaaacttg ttgcaggttt gcccaaggat
     5161 cacgatggta aggacgatca ttacttgtgg gacattggga agaagaagaa ggatgagaat
     5221 tcggttacca tccgtcagat tcttaccacg agtgccgaca cgaaggagct gaagaacgct
     5281 ggtaagtggc gcgagttttg tgagaagctt ggtgaggcgc tttatttgaa gagtaaggat
     5341 atgagcggcg ggttgaagat tacacgccgt attctcgggg acgcagagtt tcatggcaag
     5401 ccagatcgct tggaaaagtc ccgtagtgtc tcgatcggaa gcgtgttaaa ggaaaccgtg
     5461 gtgtgcggag aactcgtcgc aaagaccccg ttcttctttg gcgcgatcga cgaggatgca
     5521 aaacaaactg acttacaggt actgcttact ccggacaata agtatcgcct gccgcgcagc
     5581 gccgtccgcg gcatccttcg ccgcgatctt caaacgtact ttgacagtcc ctgtaatgcg
     5641 gagttaggcg gtcgtccatg tatgtgcaag acctgtcgta tcatgcgtgg cattacggtt
     5701 atggacgcgc gctccgagta taacgcgccc ccggagattc gtcaccgtac ccgcattaat
     5761 ccgtttacgg gaacagttgc tgagggcgcc ctgtttaata tggaagtggc cccggaaggg
     5821 atcgtattcc cattccaatt acgctatcgc ggctctgagg acgggctccc agacgcgctc
     5881 aagaccgtgc tgaagtggtg ggcagagggg caagctttta tgtcaggggc cgccagtacc
     5941 gggaaaggcc gttttcgtat ggagaatgcc aaatatgaaa ccttggatct gagcgatgag
     6001 aatcaacgca atgactactt gaagaattgg ggctggcgtg acgagaaggg tcttgaggag
     6061 cttaagaaac gtttaaattc aggtctgccg gagcctggca attaccgtga cccgaagtgg
     6121 catgaaatca acgtgtcaat cgagatggcg agtcccttca tcaacggcga ccctatccgc
     6181 gcagctgtag acaagcgtgg gacggacgtc gtgaccttcg ttaagtacaa ggcggaaggg
     6241 gaagaagcaa agcccgtgtg cgcatataag gcggagtcgt ttcgcggcgt cattcgttcg
     6301 gctgtagcac gcattcacat ggaagatgga gtgcccctga ctgagcttac tcactcagat
     6361 tgcgagtgtt tgctctgtca aatcttcggg tcggaatatg aggctggtaa gattcgtttc
     6421 gaagatttag tatttgagag tgacccggag ccggtcacct tcgatcacgt tgcaatcgat
     6481 cgcttcaccg gcggtgccgc tgacaagaag aagttcgacg acagtccttt gccagggtcg
     6541 ccggcgcgcc ctctgatgct gaagggttcc ttctggattc gccgtgacgt tctcgaagat
     6601 gaggagtatt gcaaagcgct cgggaaagca ttagccgatg taaataatgg tttatatcct
     6661 ctgggtggaa aatccgccat cggatacgga caagttaaga gcctgggtat caagggcgac
     6721 gataagcgta tcagccgttt aatgaatccg gccttcgacg aaaccgacgt ggcggttcca
     6781 gagaagccta aaaccgatgc cgaggtccgt atcgaagctg agaaggtata ttatccccac
     6841 tactttgtgg aaccgcacaa gaaggtagag cgcgaggaga agccgtgcgg tcatcaaaag
     6901 tttcatgagg gacgccttac cggtaagatt cgttgtaaac ttattacgaa aacgcctctc
     6961 attgtgccgg acaccagcaa tgatgacttc tttcgccctg cggacaaaga ggcccgcaag
     7021 gagaaggacg agtatcacaa atcctatgcc ttctttcgcc tgcataagca aatcatgatc
     7081 cccggtagtg aactccgcgg gatggtgtcc tctgtttacg aaacggttac aaattcatgt
     7141 ttccgtatct ttgacgagac taagcgtctt agttggcgca tggatgccga tcaccaaaac
     7201 gttttacaag atttcttacc gggccgtgtt acggccgacg gcaagcacat tcaaaagttt
     7261 tccgagactg cacgtgtacc gttttatgac aaaacgcaaa agcactttga cattctcgac
     7321 gagcaggaaa ttgccggtga aaagccggtc cgcatgtggg tgaagcgttt cattaagcgc
     7381 ctgagcctgg tggaccctgc aaagcaccca caaaagaagc aagacaataa gtggaaacgc
     7441 cgcaaggaag gtattgcgac gttcattgag cagaagaatg ggtcttacta ttttaacgtg
     7501 gtcaccaaca atggctgcac cagctttcac ttgtggcaca agccagacaa cttcgaccaa
     7561 gagaagttag agggcatcca aaacggggag aagttagatt gctgggttcg tgatagccgt
     7621 taccaaaagg catttcaaga aatcccggag aacgatccgg acggatggga gtgtaaggaa
     7681 ggttatctgc atgtggtggg cccttcgaag gttgaattta gcgacaagaa gggcgatgtc
     7741 attaataact tccaagggac tctcccatca gtgcccaatg attggaaaac gattcgtaca
     7801 aacgacttta agaaccgcaa acgtaagaac gagcccgttt tctgttgcga ggatgacaag
     7861 gggaactact acacgatggc caaatattgt gaaacgttct tcttcgacct gaaagagaac
     7921 gaggagtatg agatccccga gaaggcacgt atcaagtaca aggagttatt acgcgtgtac
     7981 aacaacaatc cgcaagcggt tcccgagagc gtgttccaga gtcgcgtagc gcgcgagaac
     8041 gtagaaaagc tgaagtctgg ggatttggtg tatttcaagc acaacgagaa atatgttgag
     8101 gacatcgtac cggttcgtat ttcacgtacc gtagacgacc gcatgatcgg aaagcgtatg
     8161 tcggctgatt tgcgtccatg tcacggtgac tgggtagagg acggggacct ctctgcgttg
     8221 aatgcatatc cagagaagcg ccttttattg cgtcacccta agggcctttg cccggcatgt
     8281 cgccttttcg gaacgggtag ctacaagggc cgcgttcgtt ttggcttcgc gagcctggag
     8341 aacgacccgg agtggttgat tcctggaaag aatcccggtg atccattcca cggcgggcca
     8401 gtgatgctga gcttgctgga gcgcccgcgt cccacctgga gcattcctgg cagtgataat
     8461 aagttcaaag ttccaggccg taagttctac gttcaccatc atgcttggaa aacgattaag
     8521 gacggtaacc atccgaccac cggcaaagcc attgaacaat cgccaaataa ccgcacggtt
     8581 gaagcgctcg ccggtggcaa cagcttcagc ttcgaaattg cgttcgaaaa tttgaaggag
     8641 tgggagttgg gcctgctgat ccatagcctg cagcttgaaa agggcttggc ccataagctc
     8701 ggtatggcta agagcatggg ctttggctca gtagagatcg acgttgagag tgtccgcctg
     8761 cgtaaagatt ggaagcagtg gcgcaatggt aattcagaga tcccaaattg gttgggcaag
     8821 gggtttgcaa agctgaagga gtggttccgc gacgagctgg acttcatcga gaatttgaag
     8881 aaattactgt ggttccccga aggtgatcaa gctccgcgcg tatgttaccc catgcttcgc
     8941 aagaaggatg accctaatgg aaactcaggt tatgaggagt tgaaagacgg cgaatttaag
     9001 aaggaagatc gtcagaagaa gcttacaaca ccatggacgc cgtgggcttg agcggccgca
     9061 ctcgaggccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa
     9121 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc
     9181 ggatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca
     9241 gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac
     9301 tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc
     9361 aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     9421 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     9481 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     9541 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     9601 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     9661 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     9721 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     9781 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
     9841 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     9901 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     9961 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
    10021 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
    10081 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg
    10141 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
    10201 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
    10261 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
    10321 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
    10381 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
    10441 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
    10501 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
    10561 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
    10621 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
    10681 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
    10741 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
    10801 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
    10861 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
    10921 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
    10981 ct
//
