LOCUS       sequence-394514-        9624 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial protein expression of Dcp2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9624)
  AUTHORS   Currie SL, Xing W, Muhlrad D, Decker CJ, Parker R, Rosen MK
  TITLE     Quantitative reconstitution of yeast RNA processing bodies.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Apr 4;120(14):e2214064120. doi: 
            10.1073/pnas.2214064120. Epub 2023 Mar 27.
  PUBMED    36972455
REFERENCE   2  (bases 1 to 9624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-04-4"; volume: "120(14):e2214064120. 
            doi"; pages: " 10.1073/pnas.2214064120. Epub 2023 Mar 27"
COMMENT     Sequence Label: Dcp2
FEATURES             Location/Qualifiers
     source          1..9624
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(101..106,107..122,123..129)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    137..153
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     142..164
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             223..1323
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      1180..1189
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1297..1320
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1372..1383
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1396..1416
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4336..4356
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4357..4374
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4387..4404)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4387..4403)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4396..4418)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4582..4599)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      4752..4838
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4930..4957
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        4976..5067
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5068..5136,5137..5928)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5286..5305)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(5970..6483)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6165..6186)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6377..6396
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      6594..7182
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7083..7102
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7336..7353
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7459..7478)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7619..7641
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7635..7826)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        8322..8399
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             8400..9482
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(8419..8438)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 tcactgcata attcgtgtcg ctcaaggcgc actcccgttc tggataatgt tttttgcgcc
       61 gacatcataa cggttctggc aaatattctg aaatgagctg ttgacaatta atcatcggct
      121 cgtataatgt gtggaattgt gagcggataa caatttcaca caggaaacag ccagtccgtt
      181 taggtgtttt cacgagcact tcaccaacaa ggaccataga ttatgaaaac tgaagaaggt
      241 aaactggtaa tctggattaa cggcgataaa ggctataacg gtctcgctga agtcggtaag
      301 aaattcgaga aagataccgg aattaaagtc accgttgagc atccggataa actggaagag
      361 aaattcccac aggttgcggc aactggcgat ggccctgaca ttatcttctg ggcacacgac
      421 cgctttggtg gctacgctca atctggcctg ttggctgaaa tcaccccgga caaagcgttc
      481 caggacaagc tgtatccgtt tacctgggat gccgtacgtt acaacggcaa gctgattgct
      541 tacccgatcg ctgttgaagc gttatcgctg atttataaca aagatctgct gccgaacccg
      601 ccaaaaacct gggaagagat cccggcgctg gataaagaac tgaaagcgaa aggtaagagc
      661 gcgctgatgt tcaacctgca agaaccgtac ttcacctggc cgctgattgc tgctgacggg
      721 ggttatgcgt tcaagtatga aaacggcaag tacgacatta aagacgtggg cgtggataac
      781 gctggcgcga aagcgggtct gaccttcctg gttgacctga ttaaaaacaa acacatgaat
      841 gcagacaccg attactccat cgcagaagct gcctttaata aaggcgaaac agcgatgacc
      901 atcaacggcc cgtgggcatg gtccaacatc gacaccagca aagtgaatta tggtgtaacg
      961 gtactgccga ccttcaaggg tcaaccatcc aaaccgttcg ttggcgtgct gagcgcaggt
     1021 attaacgccg ccagtccgaa caaagagctg gcaaaagagt tcctcgaaaa ctatctgctg
     1081 actgatgaag gtctggaagc ggttaataaa gacaaaccgc tgggtgccgt agcgctgaag
     1141 tcttacgagg aagagttggc gaaagatcca cgtattgccg ccaccatgga aaacgcccag
     1201 aaaggtgaaa tcatgccgaa catcccgcag atgtccgctt tctggtatgc cgtgcgtact
     1261 gcggtgatca acgccgccag cggtcgtcag actgtcgatg aagccctgaa agacgcgcag
     1321 actaattcga gctcgaacaa caacaacaat aacaataaca acaacctcgg gatcgaggga
     1381 aggatttcag aatttgaaaa cctgtatttt cagggccata tgtcactgcc gctacgacac
     1441 gcattggaga acgttacttc tgttgataga attttagagg acttattagt acgttttatt
     1501 ataaattgtc cgaatgaaga tttatcgagt gtcgagagag agttatttca ttttgaagaa
     1561 gcctcatggt tttacacgga tttcatcaaa ttgatgaatc caactttacc ctccctaaag
     1621 attaaatcat ttgctcaatt gatcataaaa ctatgtcctc tggtttggaa atgggacata
     1681 agagtggatg aggcactcca gcaattctcc aagtataaga aaagtatacc ggtgaggggc
     1741 gctgccatat ttaacgagaa cctgagtaaa attttattgg tacagggtac tgaatcggat
     1801 tcttggtcat tcccaagggg gaagatatct aaagatgaaa atgacataga ttgttgcatt
     1861 agagaagtga aagaagaaat tggtttcgat ttgacggact atattgacga caaccaattc
     1921 attgaaagaa atattcaagg taaaaattac aaaatatttt tgatatctgg tgtttcagaa
     1981 gtcttcaatt ttaaacctca agttagaaat gaaattgata agatagaatg gttcgatttt
     2041 aagaaaattt ctaaaacaat gtacaaatca aatatcaagt attatctgat taattccatg
     2101 atgagaccct tatcaatgtg gttaaggcat cagaggcaaa taaaaaatga agatcaattg
     2161 aaatcctatg cggaagaaca attgaaattg ttgttgggta tcactaagga ggagcagatt
     2221 gatcccggta gagagttgct gaatatgtta catactgcag tgcaagctaa cagtaataat
     2281 aatgcggtct ccaacggaca ggtaccctcg agccaagagc ttcagcattt gaaagagcaa
     2341 tcaggagaac acaaccaaca gaaggatcag cagtcatcgt tttcttctca acaacaacct
     2401 tcaatatttc catctctttc tgaaccgttt gctaacaata agaatgttat accacctact
     2461 atgccaatgg ctaacgtatt catgtcaaat cctcaattgt ttgcgacaat gaatggccag
     2521 ccttttgcac ctttcccatt tatgttacca ttaactaaca atagtaatag cgctaaccct
     2581 attccaactc cggtcccccc taattttaat gctcctccga atccgatggc ttttggtgtt
     2641 ccaaacatgc ataacctttc tggaccagca gtatctcaac cgttttcctt gcctcctgct
     2701 cctttaccga gggactctgg ttacagcagc tcctcccctg ggcagttgtt agatatacta
     2761 aattcgaaaa agcctgacag caacgttcaa tcaagcaaaa agccaaagct taaaatctta
     2821 cagagaggaa cggacttgaa ttcaatcaag caaaacaata atgatgaaac tgctcattca
     2881 aactctcaag ctttgctaga tttgttgaaa aaaccaacat catcgcagaa gatacacgct
     2941 tccaaaccag atacttcctt tttaccaaat gactccgtat ctggtataca agatgcagaa
     3001 tatgaagatt tcgagagtag ttcagatgaa gaggtggaga cagctagaga tgaaagaaat
     3061 tcattgaatg tagatattgg ggtgaacgtt atgccaagcg aaaaagacag ccgaagaagt
     3121 caaaaggaaa aaccaaggaa cgacgcaagc aaaacaaact tgaacgcttc tgcagaatct
     3181 aatagtgtag aatgggggcc tggtaaatct tcaccctcga ctcagagtaa acagaattca
     3241 tcagtaggaa tgcagaataa atatcgtcaa gaaattcaca ttggtgatag tgatgcgtat
     3301 gaagtcttcg aaagcagttc tgacgaagaa gatggaaaaa aattggaaga actggaacaa
     3361 actcaagaca actccaaatt aattagccaa gatattttaa aagagaacaa tttccaagac
     3421 ggtgaagtgc ctcatagaga tatgcccact gaaagtaata aatctataaa cgaaactgtc
     3481 gggttatcat caacaacaaa tactgtaaaa aaggtaccca aagttaaaat actaaaacga
     3541 ggtgaaacct ttgccagtct ggcgaatgat aaaaaagctt ttgattcaag ctccaatgtg
     3601 tcttcatcaa aagatttatt acagatgcta agaaacccaa tttcctctac tgtatcttca
     3661 aatcagcaat caccaaaatc tcaacatcta agtggggatg aagaaatcat gatgatgttg
     3721 aagaggaatt ctgtgtcaaa acctcaaaat agcgaagaaa atgcatcaac ttcaagtata
     3781 aacgatgcga atgcaagcga acttttggga atgttaaaac aaaaggaaaa agacattaca
     3841 gcaccaaaac agccctataa tgttgactca tactcgcaga aaaattcagc taaaggattg
     3901 ttgaatattt tgaaaaagaa cgactctacc gggtaccctc gcactgaggg tggaccgtca
     3961 agtgaaatgt caacgtcaat gaaacgcaac gacgctacaa ataatcaaga attagataag
     4021 aattcaacag agttattaaa ctacttaaag cccaagcctc ttaatgatgg gtatgagaat
     4081 atttcaaata aggacagttc tcatgaatta ctgaatatat tgcatggtaa taaaaacagc
     4141 agcgccttca ataataatgt atatgcaact gacggatatt ccttggcatc tgataacaac
     4201 gaaaattcct caaataaact actaaatatg ctgcaaaaca ggtctagtgc aataaatgag
     4261 cccaacttcg acgtccgttc gaatggaact tcagggtcta atgaattatt aagcattttg
     4321 cataggaagg gatccgaaaa cctgtatttt cagggccatc atcatcatca tcactgaaag
     4381 cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     4441 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     4501 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcagcttg gctgttttgg
     4561 cggatgagat aagattttca gcctgataca gattaaatca gaacgcagaa gcggtctgat
     4621 aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca cctgacccca tgccgaactc
     4681 agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga gagtagggaa
     4741 ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     4801 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg
     4861 ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact gccaggcatc
     4921 aaattaagca gaaggccatc ctgacggatg gcctttttgc gtttctacaa actcttttgt
     4981 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     5041 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     5101 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     5161 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     5221 ggtaagatcc ttgagagttt tcgccccgaa gaacgtttcc caatgatgag cacttttaaa
     5281 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     5341 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     5401 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     5461 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     5521 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     5581 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     5641 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     5701 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     5761 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     5821 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     5881 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     5941 gtttactcat atatacttta gattgattta ccccggttga taatcagaaa agccccaaaa
     6001 acaggaagat tgtataagca aatatttaaa ttgtaaacgt taatattttg ttaaaattcg
     6061 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     6121 cttataaatc aaaagaatag cccgagatag ggttgagtgt tgttccagtt tggaacaaga
     6181 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     6241 atggcccact acgtgaacca tcacccaaat caagtttttt ggggtcgagg tgccgtaaag
     6301 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     6361 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     6421 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     6481 cgtaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     6541 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     6601 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     6661 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     6721 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     6781 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     6841 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     6901 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     6961 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     7021 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     7081 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     7141 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     7201 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     7261 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     7321 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat
     7381 tttctcctta cgcatctgtg cggtatttca caccgcatgt ggtgcactct cagtacaccg
     7441 catgtggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact
     7501 ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac
     7561 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     7621 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg
     7681 taaagctcat cagcgtggtc gtgcagcgat tcacagatgt ctgcctgttc atccgcgtcc
     7741 agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta
     7801 agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg
     7861 ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa
     7921 catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac
     7981 cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca
     8041 cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac
     8101 ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag
     8161 gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc
     8221 tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc
     8281 atgcgcaccc gtggccagga cccaacgctg cccgaaattc cgacaccatc gaatggtgca
     8341 aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattcagg gtggtgaatg
     8401 tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat cagaccgttt
     8461 cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg
     8521 cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt
     8581 cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg caaattgtcg
     8641 cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac
     8701 gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg
     8761 ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa gctgcctgca
     8821 ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac agtattattt
     8881 tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg ggtcaccagc
     8941 aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct
     9001 ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa ggcgactgga
     9061 gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc gttcccactg
     9121 cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt accgagtccg
     9181 ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa gacagctcat
     9241 gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg caaaccagcg
     9301 tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg
     9361 tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc tctccccgcg
     9421 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     9481 gagcgcaacg caattaatgt aagttagctc actcattagg cacaattctc atgtttgaca
     9541 gcttatcatc gactgcacgg tgcaccaatg cttctggcgt caggcagcca tcggaagctg
     9601 tggtatggct gtgcaggtcg taaa
//
