LOCUS       sequence-394517-        8364 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial protein expression of Dcp2 1-300.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8364)
  AUTHORS   Currie SL, Xing W, Muhlrad D, Decker CJ, Parker R, Rosen MK
  TITLE     Quantitative reconstitution of yeast RNA processing bodies.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Apr 4;120(14):e2214064120. doi: 
            10.1073/pnas.2214064120. Epub 2023 Mar 27.
  PUBMED    36972455
REFERENCE   2  (bases 1 to 8364)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-04-4"; volume: "120(14):e2214064120. 
            doi"; pages: " 10.1073/pnas.2214064120. Epub 2023 Mar 27"
COMMENT     Sequence Label: Dcp2 1-300-EGFP
FEATURES             Location/Qualifiers
     source          1..8364
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(98..103,104..119,120..126)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    134..150
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     139..161
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             220..1320
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      1177..1186
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1294..1317
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1369..1380
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1393..1413
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(2344..2346,2347..2349,2350..3060)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2389..2410)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2650..2669)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2997..3018
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3073..3093
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3094..3111
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3124..3141)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3124..3140)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3133..3155)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3319..3336)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3489..3575
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3667..3694
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3713..3804
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3805..3873,3874..4665)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4023..4042)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(4707..5220)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4902..4923)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5114..5133
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      5331..5919
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5820..5839
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6073..6090
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6196..6215)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6356..6378
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6372..6563)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        7059..7136
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             7137..8219
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(7156..7175)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 ctgcataatt cgtgtcgctc aaggcgcact cccgttctgg ataatgtttt ttgcgccgac
       61 atcataacgg ttctggcaaa tattctgaaa tgagctgttg acaattaatc atcggctcgt
      121 ataatgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcca gtccgtttag
      181 gtgttttcac gagcacttca ccaacaagga ccatagatta tgaaaactga agaaggtaaa
      241 ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa
      301 ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa
      361 ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc
      421 tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag
      481 gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac
      541 ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca
      601 aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg
      661 ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt
      721 tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct
      781 ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca
      841 gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc
      901 aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta
      961 ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt
     1021 aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact
     1081 gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct
     1141 tacgaggaag agttggcgaa agatccacgt attgccgcca ccatggaaaa cgcccagaaa
     1201 ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg
     1261 gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact
     1321 aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat cgagggaagg
     1381 atttcagaat ttgaaaacct gtattttcag ggccatatgt cactgccgct acgacacgca
     1441 ttggagaacg ttacttctgt tgatagaatt ttagaggact tattagtacg ttttattata
     1501 aattgtccga atgaagattt atcgagtgtc gagagagagt tatttcattt tgaagaagcc
     1561 tcatggtttt acacggattt catcaaattg atgaatccaa ctttaccctc cctaaagatt
     1621 aaatcatttg ctcaattgat cataaaacta tgtcctctgg tttggaaatg ggacataaga
     1681 gtggatgagg cactccagca attctccaag tataagaaaa gtataccggt gaggggcgct
     1741 gccatattta acgagaacct gagtaaaatt ttattggtac agggtactga atcggattct
     1801 tggtcattcc caagggggaa gatatctaaa gatgaaaatg acatagattg ttgcattaga
     1861 gaagtgaaag aagaaattgg tttcgatttg acggactata ttgacgacaa ccaattcatt
     1921 gaaagaaata ttcaaggtaa aaattacaaa atatttttga tatctggtgt ttcagaagtc
     1981 ttcaatttta aacctcaagt tagaaatgaa attgataaga tagaatggtt cgattttaag
     2041 aaaatttcta aaacaatgta caaatcaaat atcaagtatt atctgattaa ttccatgatg
     2101 agacccttat caatgtggtt aaggcatcag aggcaaataa aaaatgaaga tcaattgaaa
     2161 tcctatgcgg aagaacaatt gaaattgttg ttgggtatca ctaaggagga gcagattgat
     2221 cccggtagag agttgctgaa tatgttacat actgcagtgc aagctaacag taataataat
     2281 gcggtctcca acggacaggt accctcgagc caagagggat ccgggtctgg ttcaggaatg
     2341 gccatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     2401 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     2461 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     2521 accctcgtga ccaccttgac ctacggcgtg cagtgcttcg cccgctaccc cgaccacatg
     2581 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     2641 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     2701 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     2761 cacaagctgg agtacaacta caacagccac aaggtctata tcaccgccga caagcagaag
     2821 aacggcatca aggtgaactt caagacccgc cacaacatcg aggacggcag cgtgcagctc
     2881 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     2941 cactacctga gcacccagtc caagctgagc aaagacccca acgagaagcg cgatcacatg
     3001 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     3061 ggaagcggcc gcgaaaacct gtattttcag ggccatcatc atcatcatca ctgaaagctt
     3121 ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     3181 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     3241 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cagcttggct gttttggcgg
     3301 atgagataag attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa
     3361 acagaatttg cctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga
     3421 agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg
     3481 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     3541 gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg
     3601 cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa
     3661 ttaagcagaa ggccatcctg acggatggcc tttttgcgtt tctacaaact cttttgttta
     3721 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     3781 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     3841 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     3901 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     3961 aagatccttg agagttttcg ccccgaagaa cgtttcccaa tgatgagcac ttttaaagtt
     4021 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
     4081 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     4141 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     4201 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     4261 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     4321 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     4381 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     4441 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     4501 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     4561 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     4621 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     4681 tactcatata tactttagat tgatttaccc cggttgataa tcagaaaagc cccaaaaaca
     4741 ggaagattgt ataagcaaat atttaaattg taaacgttaa tattttgtta aaattcgcgt
     4801 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     4861 ataaatcaaa agaatagccc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     4921 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     4981 gcccactacg tgaaccatca cccaaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     5041 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     5101 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     5161 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     5221 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     5281 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     5341 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     5401 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     5461 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     5521 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     5581 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     5641 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     5701 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     5761 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     5821 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     5881 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     5941 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     6001 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     6061 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt
     6121 ctccttacgc atctgtgcgg tatttcacac cgcatgtggt gcactctcag tacaccgcat
     6181 gtggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg
     6241 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
     6301 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     6361 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa
     6421 agctcatcag cgtggtcgtg cagcgattca cagatgtctg cctgttcatc cgcgtccagc
     6481 tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg
     6541 gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg
     6601 gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat
     6661 gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag
     6721 agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag
     6781 ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc
     6841 cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc
     6901 gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc
     6961 taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg
     7021 cgcacccgtg gccaggaccc aacgctgccc gaaattccga caccatcgaa tggtgcaaaa
     7081 cctttcgcgg tatggcatga tagcgcccgg aagagagtca attcagggtg gtgaatgtga
     7141 aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc
     7201 gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga
     7261 tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt
     7321 tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg
     7381 cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa
     7441 gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc
     7501 tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta
     7561 atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt attattttct
     7621 cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa
     7681 tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc
     7741 ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg
     7801 ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga
     7861 tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc
     7921 tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt
     7981 atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg
     8041 accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct
     8101 cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt
     8161 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     8221 cgcaacgcaa ttaatgtaag ttagctcact cattaggcac aattctcatg tttgacagct
     8281 tatcatcgac tgcacggtgc accaatgctt ctggcgtcag gcagccatcg gaagctgtgg
     8341 tatggctgtg caggtcgtaa atca
//
