LOCUS       sequence-394519-        9102 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial protein expression of Pat1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9102)
  AUTHORS   Currie SL, Xing W, Muhlrad D, Decker CJ, Parker R, Rosen MK
  TITLE     Quantitative reconstitution of yeast RNA processing bodies.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Apr 4;120(14):e2214064120. doi: 
            10.1073/pnas.2214064120. Epub 2023 Mar 27.
  PUBMED    36972455
REFERENCE   2  (bases 1 to 9102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-04-4"; volume: "120(14):e2214064120. 
            doi"; pages: " 10.1073/pnas.2214064120. Epub 2023 Mar 27"
COMMENT     Sequence Label: Pat1
FEATURES             Location/Qualifiers
     source          1..9102
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(104..109,110..125,126..132)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    140..156
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     145..167
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             226..1326
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      1183..1192
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1300..1323
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1375..1386
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1399..1419
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3817..3837
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3838..3855
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3868..3885)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3868..3884)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3877..3899)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4063..4080)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      4233..4319
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4411..4438
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        4457..4548
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4549..4617,4618..5409)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4767..4786)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(5451..5964)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5646..5667)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5858..5877
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      6075..6663
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6564..6583
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6817..6834
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6940..6959)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7100..7122
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7116..7307)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        7803..7880
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             7881..8963
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(7900..7919)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 aaatcactgc ataattcgtg tcgctcaagg cgcactcccg ttctggataa tgttttttgc
       61 gccgacatca taacggttct ggcaaatatt ctgaaatgag ctgttgacaa ttaatcatcg
      121 gctcgtataa tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagccagtcc
      181 gtttaggtgt tttcacgagc acttcaccaa caaggaccat agattatgaa aactgaagaa
      241 ggtaaactgg taatctggat taacggcgat aaaggctata acggtctcgc tgaagtcggt
      301 aagaaattcg agaaagatac cggaattaaa gtcaccgttg agcatccgga taaactggaa
      361 gagaaattcc cacaggttgc ggcaactggc gatggccctg acattatctt ctgggcacac
      421 gaccgctttg gtggctacgc tcaatctggc ctgttggctg aaatcacccc ggacaaagcg
      481 ttccaggaca agctgtatcc gtttacctgg gatgccgtac gttacaacgg caagctgatt
      541 gcttacccga tcgctgttga agcgttatcg ctgatttata acaaagatct gctgccgaac
      601 ccgccaaaaa cctgggaaga gatcccggcg ctggataaag aactgaaagc gaaaggtaag
      661 agcgcgctga tgttcaacct gcaagaaccg tacttcacct ggccgctgat tgctgctgac
      721 gggggttatg cgttcaagta tgaaaacggc aagtacgaca ttaaagacgt gggcgtggat
      781 aacgctggcg cgaaagcggg tctgaccttc ctggttgacc tgattaaaaa caaacacatg
      841 aatgcagaca ccgattactc catcgcagaa gctgccttta ataaaggcga aacagcgatg
      901 accatcaacg gcccgtgggc atggtccaac atcgacacca gcaaagtgaa ttatggtgta
      961 acggtactgc cgaccttcaa gggtcaacca tccaaaccgt tcgttggcgt gctgagcgca
     1021 ggtattaacg ccgccagtcc gaacaaagag ctggcaaaag agttcctcga aaactatctg
     1081 ctgactgatg aaggtctgga agcggttaat aaagacaaac cgctgggtgc cgtagcgctg
     1141 aagtcttacg aggaagagtt ggcgaaagat ccacgtattg ccgccaccat ggaaaacgcc
     1201 cagaaaggtg aaatcatgcc gaacatcccg cagatgtccg ctttctggta tgccgtgcgt
     1261 actgcggtga tcaacgccgc cagcggtcgt cagactgtcg atgaagccct gaaagacgcg
     1321 cagactaatt cgagctcgaa caacaacaac aataacaata acaacaacct cgggatcgag
     1381 ggaaggattt cagaatttga aaacctgtat tttcagggcc atatgtcctt ctttgggtta
     1441 gaaaatagcg gtaatgcgcg ggatggtcct ctggactttg aagagagtta caagggctat
     1501 ggcgagcacg aacttgagga gaacgactat ttgaacgacg aaacatttgg tgataatgtt
     1561 caggttggta ccgactttga ttttggaaat cctcacagca gcggcagcag cggcaacgca
     1621 attggtggta atggcgtcgg tgccacggct agatcgtatg ttgcagctac tgcagaagga
     1681 attagcggcc ctaggaccga tggaacggca gcagcaggac ctctagacct gaagccaatg
     1741 gaatctttgt ggtctactgc accacctcca gcaatggcgc cttcacccca aagtacaatg
     1801 gctccggctc ctgctccgca gcaaatggcc cccctacagc caatcttgtc gatgcaagac
     1861 ttggaaagac aacaacgtca aatgcagcaa cagtttatga atttccacgc catgggtcat
     1921 ccacagggtc tcccacaggg tccgcctcag cagcaatttc caatgcagcc tgcgtcgggt
     1981 caaccaggtc cctcacaatt tgcgcctcca cctccacctc ctggcgttaa tgtgaatatg
     2041 aatcaaatgc caatgggtcc tgtacaagtt ccagttcaag cttcgccttc acccatcggt
     2101 atgtccaaca ctccttctcc aggccctgtg gttggcgcaa ctaaaatgcc tctgcaaagt
     2161 ggacgcagat cgaagagaga tttgtcgcct gaagagcaaa gacgtttgca gattcgtcat
     2221 gccaaagtgg agaaaatctt gaaatactca ggtttaatga ctcctcgtga taaggacttc
     2281 atcaccagat atcagttgtc tcaaattgtc actgaggacc cttacaatga ggatttctac
     2341 ttccaggtct acaagattat ccaaagaggc ggtatcacgt ccgaatccaa caaaggtttg
     2401 attgctaggg cgtatttgga acattctgga cacagactcg gtggtcgcta taagagaacc
     2461 gatattgccc tacagagaat gcaaagtcaa gtagaaaagg ctgtcactgt ggctaaggaa
     2521 agaccttcta agttgaagga tcaacaagcg gctgctggta actctagcca ggataataag
     2581 caagcaaaca cggttctggg caaaatctct tccactttga acagcaagaa tccaagaaga
     2641 caactgcaga tccccagaca acagccttct gaccccgatg cgctaaaaga cgtcactgac
     2701 tctctgacca acgtggactt ggcctcttca gggtcctcct ctacgggctc ttctgccgct
     2761 gctgttgctt ctaagcaaag aagaagatct tcatacgcgt tcaacaacgg taatggtgcc
     2821 acaaatttga acaaatctgg gggcaaaaaa ttcattcttg agttaattga aacagtttat
     2881 gaagagattt tagacttgga agctaacttg aggaatggcc agcaaactga cagcactgca
     2941 atgtgggagg cccttcacat cgacgacagt tcgtatgacg taaacccttt catttcgatg
     3001 ctatcatttg ataaaggtat caagattatg cctagaattt ttaatttctt ggataagcag
     3061 caaaaattga aaatcctgca aaaaatcttc aatgaattat cacacttgca aatcatcata
     3121 ttgagttcct acaagactac accaaaacca actttgacac aattgaagaa agtcgatctg
     3181 ttccaaatga tcatattaaa gatcattgtc tcgtttttgt ctaataactc caattttatc
     3241 gaaattatgg gtctgttgct acagttaatc agaaacaaca acgtttcgtt cttgaccacc
     3301 tccaaaattg gtctaaattt gatcaccatt ttgatttctc gtgccgcatt aatcaagcaa
     3361 gattcatcaa gatctaatat tctttcctct cctgaaatct ccacatggaa tgagatttat
     3421 gataaattat tcacttcatt ggaaagtaag attcagctga ttttccctcc aagggaatat
     3481 aacgaccaca tcatgcgttt acaaaatgac aagtttatgg atgaagcata catttggcag
     3541 ttcctagcta gtttagcact aagtggaaag ctaaaccacc agagaatcat tattgatgaa
     3601 gtacgtgatg aaatctttgc cactattaac gaggcggaga ccttacaaaa gaaagagaaa
     3661 gaattgagtg tattacctca gaggtctcaa gaattagaca cagagttaaa atctattatt
     3721 tataataaag agaaactata ccaagatttg aatttgttcc taaacgttat ggggttggtg
     3781 tatcgcgatg gtgaaatatc agaactaaag ggatccgaaa acctgtattt tcagggccat
     3841 catcatcatc atcactgaaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga
     3901 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     3961 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     4021 atggcagctt ggctgttttg gcggatgaga taagattttc agcctgatac agattaaatc
     4081 agaacgcaga agcggtctga taaaacagaa tttgcctggc ggcagtagcg cggtggtccc
     4141 acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta gtgtggggtc
     4201 tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct cagtcgaaag
     4261 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc
     4321 cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc
     4381 cgccataaac tgccaggcat caaattaagc agaaggccat cctgacggat ggcctttttg
     4441 cgtttctaca aactcttttg tttatttttc taaatacatt caaatatgta tccgctcatg
     4501 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     4561 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     4621 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     4681 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttc
     4741 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
     4801 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     4861 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     4921 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     4981 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     5041 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     5101 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     5161 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     5221 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     5281 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     5341 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     5401 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt accccggttg
     5461 ataatcagaa aagccccaaa aacaggaaga ttgtataagc aaatatttaa attgtaaacg
     5521 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     5581 aggccgaaat cggcaaaatc ccttataaat caaaagaata gcccgagata gggttgagtg
     5641 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     5701 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcacccaaa tcaagttttt
     5761 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     5821 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     5881 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     5941 ttaatgcgcc gctacagggc gcgtaaaagg atctaggtga agatcctttt tgataatctc
     6001 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     6061 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6121 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     6181 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     6241 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     6301 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     6361 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     6421 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     6481 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6541 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     6601 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6661 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6721 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     6781 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     6841 gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcatg
     6901 tggtgcactc tcagtacacc gcatgtggtg cactctcagt acaatctgct ctgatgccgc
     6961 atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
     7021 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     7081 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     7141 aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgcagcga ttcacagatg
     7201 tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt
     7261 ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg
     7321 taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg
     7381 atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg
     7441 gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt
     7501 aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac
     7561 ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag
     7621 accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc
     7681 tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc
     7741 ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgaaatt
     7801 ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga
     7861 gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg
     7921 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa
     7981 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac
     8041 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc
     8101 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg
     8161 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc
     8221 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca
     8281 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga
     8341 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc
     8401 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg
     8461 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag
     8521 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga
     8581 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa
     8641 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg
     8701 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc
     8761 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga
     8821 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata
     8881 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     8941 cccgactgga aagcgggcag tgagcgcaac gcaattaatg taagttagct cactcattag
     9001 gcacaattct catgtttgac agcttatcat cgactgcacg gtgcaccaat gcttctggcg
     9061 tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gt
//
