LOCUS       sequence-394521-        8157 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial protein expression of Dcp1 H40A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Currie SL, Xing W, Muhlrad D, Decker CJ, Parker R, Rosen MK
  TITLE     Quantitative reconstitution of yeast RNA processing bodies.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Apr 4;120(14):e2214064120. doi: 
            10.1073/pnas.2214064120. Epub 2023 Mar 27.
  PUBMED    36972455
REFERENCE   2  (bases 1 to 8157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-04-4"; volume: "120(14):e2214064120. 
            doi"; pages: " 10.1073/pnas.2214064120. Epub 2023 Mar 27"
COMMENT     Sequence Label: Dcp1 H40A-EGFP
FEATURES             Location/Qualifiers
     source          1..8157
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(79..84,85..100,101..107)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    115..131
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     120..142
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             201..1301
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      1158..1167
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1275..1298
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1350..1361
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1374..1394
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(2118..2120,2121..2123,2124..2834)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2163..2184)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2424..2443)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2771..2792
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2847..2867
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2868..2885
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2898..2914)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3093..3110)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3263..3349
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3441..3468
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3487..3578
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3579..3647,3648..4439)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3797..3816)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(4481..4994)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4676..4697)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4888..4907
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      5105..5693
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5594..5613
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5847..5864
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5970..5989)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6130..6152
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6146..6337)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        6833..6910
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             6911..7993
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(6930..6949)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 caaggcgcac tcccgttctg gataatgttt tttgcgccga catcataacg gttctggcaa
       61 atattctgaa atgagctgtt gacaattaat catcggctcg tataatgtgt ggaattgtga
      121 gcggataaca atttcacaca ggaaacagcc agtccgttta ggtgttttca cgagcacttc
      181 accaacaagg accatagatt atgaaaactg aagaaggtaa actggtaatc tggattaacg
      241 gcgataaagg ctataacggt ctcgctgaag tcggtaagaa attcgagaaa gataccggaa
      301 ttaaagtcac cgttgagcat ccggataaac tggaagagaa attcccacag gttgcggcaa
      361 ctggcgatgg ccctgacatt atcttctggg cacacgaccg ctttggtggc tacgctcaat
      421 ctggcctgtt ggctgaaatc accccggaca aagcgttcca ggacaagctg tatccgttta
      481 cctgggatgc cgtacgttac aacggcaagc tgattgctta cccgatcgct gttgaagcgt
      541 tatcgctgat ttataacaaa gatctgctgc cgaacccgcc aaaaacctgg gaagagatcc
      601 cggcgctgga taaagaactg aaagcgaaag gtaagagcgc gctgatgttc aacctgcaag
      661 aaccgtactt cacctggccg ctgattgctg ctgacggggg ttatgcgttc aagtatgaaa
      721 acggcaagta cgacattaaa gacgtgggcg tggataacgc tggcgcgaaa gcgggtctga
      781 ccttcctggt tgacctgatt aaaaacaaac acatgaatgc agacaccgat tactccatcg
      841 cagaagctgc ctttaataaa ggcgaaacag cgatgaccat caacggcccg tgggcatggt
      901 ccaacatcga caccagcaaa gtgaattatg gtgtaacggt actgccgacc ttcaagggtc
      961 aaccatccaa accgttcgtt ggcgtgctga gcgcaggtat taacgccgcc agtccgaaca
     1021 aagagctggc aaaagagttc ctcgaaaact atctgctgac tgatgaaggt ctggaagcgg
     1081 ttaataaaga caaaccgctg ggtgccgtag cgctgaagtc ttacgaggaa gagttggcga
     1141 aagatccacg tattgccgcc accatggaaa acgcccagaa aggtgaaatc atgccgaaca
     1201 tcccgcagat gtccgctttc tggtatgccg tgcgtactgc ggtgatcaac gccgccagcg
     1261 gtcgtcagac tgtcgatgaa gccctgaaag acgcgcagac taattcgagc tcgaacaaca
     1321 acaacaataa caataacaac aacctcggga tcgagggaag gatttcagaa tttgaaaacc
     1381 tgtattttca gggccatatg accggagcag caactgcagc agaaaactct gccacacagt
     1441 tagaattcta tagaaaagct ttgaatttca acgttattgg gagatacgat ccaaaaataa
     1501 agcaactact ttttgccaca ccacatgcgt cactgtataa atgggacttc aagaaggacg
     1561 aatggaataa actagaatat caaggtgttt tggccatata tttgagagac gtctcgcaaa
     1621 atacaaatct tctacccgtc tccccacaag aagtagacat ttttgattca caaaatggta
     1681 gtaataacat tcaagtaaac aatggttctg acaatagcaa caggaatagc agtggaaatg
     1741 ggaactctta taaaagtaat gattcgctaa catataattg cggcaaaact ttaagtggaa
     1801 aggacatata caattatgga ttgatcatat taaaccgaat taatcctgac aatttttcta
     1861 tgggcatcgt tcccaatagt gtcgtaaata aaagaaaagt atttaatgca gaagaggata
     1921 cacttaaccc actagagtgc atgggggtag aggtgaaaga tgaattggtc attattaaga
     1981 acttgaagca tgaggtctat ggtatatgga ttcatacagt cagtgataga caaaatatct
     2041 acgaactaat aaaatatctt ctggaaaatg agccaaaaga ttcttttgct ggatccgggt
     2101 ctggttcagg aatggccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     2161 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     2221 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     2281 ccgtgccctg gcccaccctc gtgaccacct tgacctacgg cgtgcagtgc ttcgcccgct
     2341 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     2401 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     2461 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     2521 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaaggtc tatatcaccg
     2581 ccgacaagca gaagaacggc atcaaggtga acttcaagac ccgccacaac atcgaggacg
     2641 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     2701 tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac cccaacgaga
     2761 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     2821 acgagctgta caagggaagc ggccgcgaaa acctgtattt tcagggccat catcatcatc
     2881 atcactgaaa gcttggcact ggccgtcgtt ttacgacgtc gtgactggga aaaccctggc
     2941 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     3001 gaggcccgca ccgatcgctc ttcccaacag ttgcgcagcc tgaatggcga atggcagctt
     3061 ggctgttttg gcggatgaga taagattttc agcctgatac agattaaatc agaacgcaga
     3121 agcggtctga taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc
     3181 atgccgaact cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg
     3241 agagtaggga actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     3301 tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc
     3361 ggatttgaac gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac
     3421 tgccaggcat caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca
     3481 aactcttttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     3541 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     3601 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     3661 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     3721 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttc ccaatgatga
     3781 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc
     3841 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     3901 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     3961 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     4021 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     4081 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     4141 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     4201 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     4261 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     4321 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     4381 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     4441 tgtcagacca agtttactca tatatacttt agattgattt accccggttg ataatcagaa
     4501 aagccccaaa aacaggaaga ttgtataagc aaatatttaa attgtaaacg ttaatatttt
     4561 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     4621 cggcaaaatc ccttataaat caaaagaata gcccgagata gggttgagtg ttgttccagt
     4681 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     4741 ctatcagggc gatggcccac tacgtgaacc atcacccaaa tcaagttttt tggggtcgag
     4801 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     4861 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     4921 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     4981 gctacagggc gcgtaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     5041 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     5101 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     5161 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     5221 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     5281 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     5341 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     5401 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     5461 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     5521 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     5581 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     5641 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     5701 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     5761 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     5821 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     5881 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcatg tggtgcactc
     5941 tcagtacacc gcatgtggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     6001 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa
     6061 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     6121 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     6181 ggcagctgcg gtaaagctca tcagcgtggt cgtgcagcga ttcacagatg tctgcctgtt
     6241 catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc
     6301 gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat
     6361 ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta
     6421 ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga
     6481 tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg
     6541 taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc
     6601 agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg
     6661 ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg
     6721 gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca
     6781 ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgaaatt ccgacaccat
     6841 cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcag
     6901 ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta
     6961 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa
     7021 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc
     7081 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc
     7141 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc
     7201 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca
     7261 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga
     7321 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa
     7381 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt
     7441 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg
     7501 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga
     7561 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat
     7621 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat
     7681 taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga
     7741 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg
     7801 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca
     7861 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc
     7921 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     7981 aagcgggcag tgagcgcaac gcaattaatg taagttagct cactcattag gcacaattct
     8041 catgtttgac agcttatcat cgactgcacg gtgcaccaat gcttctggcg tcaggcagcc
     8101 atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg tgtcgct
//
