LOCUS       sequence-394524-        9852 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial protein expression of Pat1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9852)
  AUTHORS   Currie SL, Xing W, Muhlrad D, Decker CJ, Parker R, Rosen MK
  TITLE     Quantitative reconstitution of yeast RNA processing bodies.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Apr 4;120(14):e2214064120. doi: 
            10.1073/pnas.2214064120. Epub 2023 Mar 27.
  PUBMED    36972455
REFERENCE   2  (bases 1 to 9852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-04-4"; volume: "120(14):e2214064120. 
            doi"; pages: " 10.1073/pnas.2214064120. Epub 2023 Mar 27"
COMMENT     Sequence Label: Pat1-EGFP
FEATURES             Location/Qualifiers
     source          1..9852
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(104..109,110..125,126..132)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    140..156
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     145..167
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             226..1326
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      1183..1192
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1300..1323
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1375..1386
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1399..1419
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(3838..3840,3841..3843,3844..4554)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3883..3904)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4144..4163)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4491..4512
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4567..4587
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4588..4605
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4618..4635)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4618..4634)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4627..4649)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4813..4830)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      4983..5069
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5161..5188
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        5207..5298
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5299..5367,5368..6159)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5517..5536)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(6201..6714)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6396..6417)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6608..6627
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      6825..7413
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7314..7333
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7567..7584
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7690..7709)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7850..7872
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7866..8057)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        8553..8630
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             8631..9713
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(8650..8669)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 aaatcactgc ataattcgtg tcgctcaagg cgcactcccg ttctggataa tgttttttgc
       61 gccgacatca taacggttct ggcaaatatt ctgaaatgag ctgttgacaa ttaatcatcg
      121 gctcgtataa tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagccagtcc
      181 gtttaggtgt tttcacgagc acttcaccaa caaggaccat agattatgaa aactgaagaa
      241 ggtaaactgg taatctggat taacggcgat aaaggctata acggtctcgc tgaagtcggt
      301 aagaaattcg agaaagatac cggaattaaa gtcaccgttg agcatccgga taaactggaa
      361 gagaaattcc cacaggttgc ggcaactggc gatggccctg acattatctt ctgggcacac
      421 gaccgctttg gtggctacgc tcaatctggc ctgttggctg aaatcacccc ggacaaagcg
      481 ttccaggaca agctgtatcc gtttacctgg gatgccgtac gttacaacgg caagctgatt
      541 gcttacccga tcgctgttga agcgttatcg ctgatttata acaaagatct gctgccgaac
      601 ccgccaaaaa cctgggaaga gatcccggcg ctggataaag aactgaaagc gaaaggtaag
      661 agcgcgctga tgttcaacct gcaagaaccg tacttcacct ggccgctgat tgctgctgac
      721 gggggttatg cgttcaagta tgaaaacggc aagtacgaca ttaaagacgt gggcgtggat
      781 aacgctggcg cgaaagcggg tctgaccttc ctggttgacc tgattaaaaa caaacacatg
      841 aatgcagaca ccgattactc catcgcagaa gctgccttta ataaaggcga aacagcgatg
      901 accatcaacg gcccgtgggc atggtccaac atcgacacca gcaaagtgaa ttatggtgta
      961 acggtactgc cgaccttcaa gggtcaacca tccaaaccgt tcgttggcgt gctgagcgca
     1021 ggtattaacg ccgccagtcc gaacaaagag ctggcaaaag agttcctcga aaactatctg
     1081 ctgactgatg aaggtctgga agcggttaat aaagacaaac cgctgggtgc cgtagcgctg
     1141 aagtcttacg aggaagagtt ggcgaaagat ccacgtattg ccgccaccat ggaaaacgcc
     1201 cagaaaggtg aaatcatgcc gaacatcccg cagatgtccg ctttctggta tgccgtgcgt
     1261 actgcggtga tcaacgccgc cagcggtcgt cagactgtcg atgaagccct gaaagacgcg
     1321 cagactaatt cgagctcgaa caacaacaac aataacaata acaacaacct cgggatcgag
     1381 ggaaggattt cagaatttga aaacctgtat tttcagggcc atatgtcctt ctttgggtta
     1441 gaaaatagcg gtaatgcgcg ggatggtcct ctggactttg aagagagtta caagggctat
     1501 ggcgagcacg aacttgagga gaacgactat ttgaacgacg aaacatttgg tgataatgtt
     1561 caggttggta ccgactttga ttttggaaat cctcacagca gcggcagcag cggcaacgca
     1621 attggtggta atggcgtcgg tgccacggct agatcgtatg ttgcagctac tgcagaagga
     1681 attagcggcc ctaggaccga tggaacggca gcagcaggac ctctagacct gaagccaatg
     1741 gaatctttgt ggtctactgc accacctcca gcaatggcgc cttcacccca aagtacaatg
     1801 gctccggctc ctgctccgca gcaaatggcc cccctacagc caatcttgtc gatgcaagac
     1861 ttggaaagac aacaacgtca aatgcagcaa cagtttatga atttccacgc catgggtcat
     1921 ccacagggtc tcccacaggg tccgcctcag cagcaatttc caatgcagcc tgcgtcgggt
     1981 caaccaggtc cctcacaatt tgcgcctcca cctccacctc ctggcgttaa tgtgaatatg
     2041 aatcaaatgc caatgggtcc tgtacaagtt ccagttcaag cttcgccttc acccatcggt
     2101 atgtccaaca ctccttctcc aggccctgtg gttggcgcaa ctaaaatgcc tctgcaaagt
     2161 ggacgcagat cgaagagaga tttgtcgcct gaagagcaaa gacgtttgca gattcgtcat
     2221 gccaaagtgg agaaaatctt gaaatactca ggtttaatga ctcctcgtga taaggacttc
     2281 atcaccagat atcagttgtc tcaaattgtc actgaggacc cttacaatga ggatttctac
     2341 ttccaggtct acaagattat ccaaagaggc ggtatcacgt ccgaatccaa caaaggtttg
     2401 attgctaggg cgtatttgga acattctgga cacagactcg gtggtcgcta taagagaacc
     2461 gatattgccc tacagagaat gcaaagtcaa gtagaaaagg ctgtcactgt ggctaaggaa
     2521 agaccttcta agttgaagga tcaacaagcg gctgctggta actctagcca ggataataag
     2581 caagcaaaca cggttctggg caaaatctct tccactttga acagcaagaa tccaagaaga
     2641 caactgcaga tccccagaca acagccttct gaccccgatg cgctaaaaga cgtcactgac
     2701 tctctgacca acgtggactt ggcctcttca gggtcctcct ctacgggctc ttctgccgct
     2761 gctgttgctt ctaagcaaag aagaagatct tcatacgcgt tcaacaacgg taatggtgcc
     2821 acaaatttga acaaatctgg gggcaaaaaa ttcattcttg agttaattga aacagtttat
     2881 gaagagattt tagacttgga agctaacttg aggaatggcc agcaaactga cagcactgca
     2941 atgtgggagg cccttcacat cgacgacagt tcgtatgacg taaacccttt catttcgatg
     3001 ctatcatttg ataaaggtat caagattatg cctagaattt ttaatttctt ggataagcag
     3061 caaaaattga aaatcctgca aaaaatcttc aatgaattat cacacttgca aatcatcata
     3121 ttgagttcct acaagactac accaaaacca actttgacac aattgaagaa agtcgatctg
     3181 ttccaaatga tcatattaaa gatcattgtc tcgtttttgt ctaataactc caattttatc
     3241 gaaattatgg gtctgttgct acagttaatc agaaacaaca acgtttcgtt cttgaccacc
     3301 tccaaaattg gtctaaattt gatcaccatt ttgatttctc gtgccgcatt aatcaagcaa
     3361 gattcatcaa gatctaatat tctttcctct cctgaaatct ccacatggaa tgagatttat
     3421 gataaattat tcacttcatt ggaaagtaag attcagctga ttttccctcc aagggaatat
     3481 aacgaccaca tcatgcgttt acaaaatgac aagtttatgg atgaagcata catttggcag
     3541 ttcctagcta gtttagcact aagtggaaag ctaaaccacc agagaatcat tattgatgaa
     3601 gtacgtgatg aaatctttgc cactattaac gaggcggaga ccttacaaaa gaaagagaaa
     3661 gaattgagtg tattacctca gaggtctcaa gaattagaca cagagttaaa atctattatt
     3721 tataataaag agaaactata ccaagatttg aatttgttcc taaacgttat ggggttggtg
     3781 tatcgcgatg gtgaaatatc agaactaaag ggatccgggt ctggttcagg aatggccatg
     3841 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     3901 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     3961 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     4021 gtgaccacct tgacctacgg cgtgcagtgc ttcgcccgct accccgacca catgaagcag
     4081 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     4141 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     4201 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     4261 ctggagtaca actacaacag ccacaaggtc tatatcaccg ccgacaagca gaagaacggc
     4321 atcaaggtga acttcaagac ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     4381 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     4441 ctgagcaccc agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     4501 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagggaagc
     4561 ggccgcgaaa acctgtattt tcagggccat catcatcatc atcactgaaa gcttggcact
     4621 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     4681 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     4741 ttcccaacag ttgcgcagcc tgaatggcga atggcagctt ggctgttttg gcggatgaga
     4801 taagattttc agcctgatac agattaaatc agaacgcaga agcggtctga taaaacagaa
     4861 tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa
     4921 acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga actgccaggc
     4981 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     5041 cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc
     5101 aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat caaattaagc
     5161 agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg tttatttttc
     5221 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     5281 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     5341 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     5401 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     5461 cttgagagtt ttcgccccga agaacgtttc ccaatgatga gcacttttaa agttctgcta
     5521 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
     5581 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     5641 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     5701 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     5761 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     5821 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     5881 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     5941 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     6001 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     6061 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     6121 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     6181 tatatacttt agattgattt accccggttg ataatcagaa aagccccaaa aacaggaaga
     6241 ttgtataagc aaatatttaa attgtaaacg ttaatatttt gttaaaattc gcgttaaatt
     6301 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     6361 caaaagaata gcccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     6421 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     6481 tacgtgaacc atcacccaaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     6541 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     6601 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     6661 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtaaaagg
     6721 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     6781 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6841 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6901 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6961 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     7021 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     7081 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     7141 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     7201 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     7261 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     7321 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     7381 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     7441 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     7501 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     7561 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt
     7621 acgcatctgt gcggtatttc acaccgcatg tggtgcactc tcagtacacc gcatgtggtg
     7681 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg
     7741 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
     7801 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     7861 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca
     7921 tcagcgtggt cgtgcagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg
     7981 agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt
     8041 ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg
     8101 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
     8161 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
     8221 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc
     8281 cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt
     8341 tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac
     8401 gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca
     8461 gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc
     8521 cgtggccagg acccaacgct gcccgaaatt ccgacaccat cgaatggtgc aaaacctttc
     8581 gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag
     8641 taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg
     8701 tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg
     8761 agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga
     8821 ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta
     8881 aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg
     8941 tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca
     9001 ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc
     9061 cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg
     9121 aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc
     9181 tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat
     9241 atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt
     9301 ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg
     9361 ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg
     9421 ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc
     9481 cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct
     9541 tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg
     9601 tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     9661 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     9721 gcaattaatg taagttagct cactcattag gcacaattct catgtttgac agcttatcat
     9781 cgactgcacg gtgcaccaat gcttctggcg tcaggcagcc atcggaagct gtggtatggc
     9841 tgtgcaggtc gt
//
