LOCUS       sequence-394525-        8157 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial protein expression of Dcp1 H43A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Currie SL, Xing W, Muhlrad D, Decker CJ, Parker R, Rosen MK
  TITLE     Quantitative reconstitution of yeast RNA processing bodies.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Apr 4;120(14):e2214064120. doi: 
            10.1073/pnas.2214064120. Epub 2023 Mar 27.
  PUBMED    36972455
REFERENCE   2  (bases 1 to 8157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-04-4"; volume: "120(14):e2214064120. 
            doi"; pages: " 10.1073/pnas.2214064120. Epub 2023 Mar 27"
COMMENT     Sequence Label: Dcp1 H43A-EGFP
FEATURES             Location/Qualifiers
     source          1..8157
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(104..109,110..125,126..132)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    140..156
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     145..167
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             226..1326
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVVATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      1183..1192
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1300..1323
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1375..1386
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1399..1419
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(2143..2145,2146..2148,2149..2859)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2188..2209)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2449..2468)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2796..2817
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2872..2892
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2893..2910
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2923..2940)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2923..2939)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2932..2954)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3118..3135)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3288..3374
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3466..3493
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3512..3603
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3604..3672,3673..4464)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3822..3841)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(4506..5019)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4701..4722)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4913..4932
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      5130..5718
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5619..5638
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5872..5889
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5995..6014)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6155..6177
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6171..6362)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        6858..6935
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             6936..8018
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(6955..6974)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 aaatcactgc ataattcgtg tcgctcaagg cgcactcccg ttctggataa tgttttttgc
       61 gccgacatca taacggttct ggcaaatatt ctgaaatgag ctgttgacaa ttaatcatcg
      121 gctcgtataa tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagccagtcc
      181 gtttaggtgt tttcacgagc acttcaccaa caaggaccat agattatgaa aactgaagaa
      241 ggtaaactgg taatctggat taacggcgat aaaggctata acggtctcgc tgaagtcggt
      301 aagaaattcg agaaagatac cggaattaaa gtcaccgttg agcatccgga taaactggaa
      361 gagaaattcc cacaggttgt ggcaactggc gatggccctg acattatctt ctgggcacac
      421 gaccgctttg gtggctacgc tcaatctggc ctgttggctg aaatcacccc ggacaaagcg
      481 ttccaggaca agctgtatcc gtttacctgg gatgccgtac gttacaacgg caagctgatt
      541 gcttacccga tcgctgttga agcgttatcg ctgatttata acaaagatct gctgccgaac
      601 ccgccaaaaa cctgggaaga gatcccggcg ctggataaag aactgaaagc gaaaggtaag
      661 agcgcgctga tgttcaacct gcaagaaccg tacttcacct ggccgctgat tgctgctgac
      721 gggggttatg cgttcaagta tgaaaacggc aagtacgaca ttaaagacgt gggcgtggat
      781 aacgctggcg cgaaagcggg tctgaccttc ctggttgacc tgattaaaaa caaacacatg
      841 aatgcagaca ccgattactc catcgcagaa gctgccttta ataaaggcga aacagcgatg
      901 accatcaacg gcccgtgggc atggtccaac atcgacacca gcaaagtgaa ttatggtgta
      961 acggtactgc cgaccttcaa gggtcaacca tccaaaccgt tcgttggcgt gctgagcgca
     1021 ggtattaacg ccgccagtcc gaacaaagag ctggcaaaag agttcctcga aaactatctg
     1081 ctgactgatg aaggtctgga agcggttaat aaagacaaac cgctgggtgc cgtagcgctg
     1141 aagtcttacg aggaagagtt ggcgaaagat ccacgtattg ccgccaccat ggaaaacgcc
     1201 cagaaaggtg aaatcatgcc gaacatcccg cagatgtccg ctttctggta tgccgtgcgt
     1261 actgcggtga tcaacgccgc cagcggtcgt cagactgtcg atgaagccct gaaagacgcg
     1321 cagactaatt cgagctcgaa caacaacaac aataacaata acaacaacct cgggatcgag
     1381 ggaaggattt cagaatttga aaacctgtat tttcagggcc atatgaccgg agcagcaact
     1441 gcagcagaaa actctgccac acagttagaa ttctatagaa aagctttgaa tttcaacgtt
     1501 attgggagat acgatccaaa aataaagcaa ctactttttc acacaccagc tgcgtcactg
     1561 tataaatggg acttcaagaa ggacgaatgg aataaactag aatatcaagg tgttttggcc
     1621 atatatttga gagacgtctc gcaaaataca aatcttctac ccgtctcccc acaagaagta
     1681 gacatttttg attcacaaaa tggtagtaat aacattcaag taaacaatgg ttctgacaat
     1741 agcaacagga atagcagtgg aaatgggaac tcttataaaa gtaatgattc gctaacatat
     1801 aattgcggca aaactttaag tggaaaggac atatacaatt atggattgat catattaaac
     1861 cgaattaatc ctgacaattt ttctatgggc atcgttccca atagtgtcgt aaataaaaga
     1921 aaagtattta atgcagaaga ggatacactt aacccactag agtgcatggg ggtagaggtg
     1981 aaagatgaat tggtcattat taagaacttg aagcatgagg tctatggtat atggattcat
     2041 acagtcagtg atagacaaaa tatctacgaa ctaataaaat atcttctgga aaatgagcca
     2101 aaagattctt ttgctggatc cgggtctggt tcaggaatgg ccatggtgag caagggcgag
     2161 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     2221 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     2281 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttgacc
     2341 tacggcgtgc agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag
     2401 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     2461 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     2521 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     2581 aacagccaca aggtctatat caccgccgac aagcagaaga acggcatcaa ggtgaacttc
     2641 aagacccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     2701 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     2761 aagctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     2821 gccgccggga tcactctcgg catggacgag ctgtacaagg gaagcggccg cgaaaacctg
     2881 tattttcagg gccatcatca tcatcatcac tgaaagcttg gcactggccg tcgttttaca
     2941 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     3001 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     3061 cagcctgaat ggcgaatggc agcttggctg ttttggcgga tgagataaga ttttcagcct
     3121 gatacagatt aaatcagaac gcagaagcgg tctgataaaa cagaatttgc ctggcggcag
     3181 tagcgcggtg gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga
     3241 tggtagtgtg gggtctcccc atgcgagagt agggaactgc caggcatcaa ataaaacgaa
     3301 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc
     3361 tgagtaggac aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg cccggagggt
     3421 ggcgggcagg acgcccgcca taaactgcca ggcatcaaat taagcagaag gccatcctga
     3481 cggatggcct ttttgcgttt ctacaaactc ttttgtttat ttttctaaat acattcaaat
     3541 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     3601 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     3661 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     3721 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     3781 cccgaagaac gtttcccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     3841 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     3901 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     3961 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     4021 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     4081 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     4141 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     4201 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     4261 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     4321 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     4381 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     4441 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     4501 gatttacccc ggttgataat cagaaaagcc ccaaaaacag gaagattgta taagcaaata
     4561 tttaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     4621 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagcccg
     4681 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     4741 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     4801 ccaaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     4861 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     4921 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     4981 ccacacccgc cgcgcttaat gcgccgctac agggcgcgta aaaggatcta ggtgaagatc
     5041 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     5101 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     5161 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     5221 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     5281 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     5341 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     5401 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     5461 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     5521 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     5581 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     5641 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     5701 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     5761 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     5821 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     5881 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     5941 atttcacacc gcatgtggtg cactctcagt acaccgcatg tggtgcactc tcagtacaat
     6001 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     6061 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     6121 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     6181 tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtgc
     6241 agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc
     6301 gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc
     6361 actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga
     6421 gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt
     6481 gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa
     6541 tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg
     6601 atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa
     6661 acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag
     6721 tcgcttcacg ttcgctcgcg tatcggtgat tcattatgct aaccagtaag gcaaccccgc
     6781 cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca
     6841 acgctgcccg aaattccgac accatcgaat ggtgcaaaac ctttcgcggt atggcatgat
     6901 agcgcccgga agagagtcaa ttcagggtgg tgaatgtgaa accagtaacg ttatacgatg
     6961 tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac caggccagcc
     7021 acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc
     7081 ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct
     7141 ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc
     7201 aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag
     7261 cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg
     7321 atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg ttatttcttg
     7381 atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac ggtacgcgac
     7441 tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat
     7501 taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc actcgcaatc
     7561 aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt tttcaacaaa
     7621 ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc aacgatcaga
     7681 tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggatatct
     7741 cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg ttaaccacca
     7801 tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc
     7861 agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca
     7921 ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     7981 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtaagt
     8041 tagctcactc attaggcaca attctcatgt ttgacagctt atcatcgact gcacggtgca
     8101 ccaatgcttc tggcgtcagg cagccatcgg aagctgtggt atggctgtgc aggtcgt
//
