LOCUS       sequence-394526-        8157 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial protein expression of Dcp1 H198A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Currie SL, Xing W, Muhlrad D, Decker CJ, Parker R, Rosen MK
  TITLE     Quantitative reconstitution of yeast RNA processing bodies.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Apr 4;120(14):e2214064120. doi: 
            10.1073/pnas.2214064120. Epub 2023 Mar 27.
  PUBMED    36972455
REFERENCE   2  (bases 1 to 8157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-04-4"; volume: "120(14):e2214064120. 
            doi"; pages: " 10.1073/pnas.2214064120. Epub 2023 Mar 27"
COMMENT     Sequence Label: Dcp1 H198A-EGFP
FEATURES             Location/Qualifiers
     source          1..8157
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(92..97,98..113,114..120)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    128..144
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     133..155
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             214..1314
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      1171..1180
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1288..1311
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1363..1374
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1387..1407
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(2131..2133,2134..2136,2137..2847)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2176..2197)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2437..2456)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2784..2805
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2860..2880
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2881..2898
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2911..2928)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2911..2927)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2920..2942)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3106..3123)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3276..3362
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3454..3481
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3500..3591
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3592..3660,3661..4452)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3810..3829)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(4494..5007)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4689..4710)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4901..4920
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      5118..5706
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5607..5626
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5860..5877
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5983..6002)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6143..6165
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6159..6350)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        6846..6923
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             6924..8006
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(6943..6962)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 aattcgtgtc gctcaaggcg cactcccgtt ctggataatg ttttttgcgc cgacatcata
       61 acggttctgg caaatattct gaaatgagct gttgacaatt aatcatcggc tcgtataatg
      121 tgtggaattg tgagcggata acaatttcac acaggaaaca gccagtccgt ttaggtgttt
      181 tcacgagcac ttcaccaaca aggaccatag attatgaaaa ctgaagaagg taaactggta
      241 atctggatta acggcgataa aggctataac ggtctcgctg aagtcggtaa gaaattcgag
      301 aaagataccg gaattaaagt caccgttgag catccggata aactggaaga gaaattccca
      361 caggttgcgg caactggcga tggccctgac attatcttct gggcacacga ccgctttggt
      421 ggctacgctc aatctggcct gttggctgaa atcaccccgg acaaagcgtt ccaggacaag
      481 ctgtatccgt ttacctggga tgccgtacgt tacaacggca agctgattgc ttacccgatc
      541 gctgttgaag cgttatcgct gatttataac aaagatctgc tgccgaaccc gccaaaaacc
      601 tgggaagaga tcccggcgct ggataaagaa ctgaaagcga aaggtaagag cgcgctgatg
      661 ttcaacctgc aagaaccgta cttcacctgg ccgctgattg ctgctgacgg gggttatgcg
      721 ttcaagtatg aaaacggcaa gtacgacatt aaagacgtgg gcgtggataa cgctggcgcg
      781 aaagcgggtc tgaccttcct ggttgacctg attaaaaaca aacacatgaa tgcagacacc
      841 gattactcca tcgcagaagc tgcctttaat aaaggcgaaa cagcgatgac catcaacggc
      901 ccgtgggcat ggtccaacat cgacaccagc aaagtgaatt atggtgtaac ggtactgccg
      961 accttcaagg gtcaaccatc caaaccgttc gttggcgtgc tgagcgcagg tattaacgcc
     1021 gccagtccga acaaagagct ggcaaaagag ttcctcgaaa actatctgct gactgatgaa
     1081 ggtctggaag cggttaataa agacaaaccg ctgggtgccg tagcgctgaa gtcttacgag
     1141 gaagagttgg cgaaagatcc acgtattgcc gccaccatgg aaaacgccca gaaaggtgaa
     1201 atcatgccga acatcccgca gatgtccgct ttctggtatg ccgtgcgtac tgcggtgatc
     1261 aacgccgcca gcggtcgtca gactgtcgat gaagccctga aagacgcgca gactaattcg
     1321 agctcgaaca acaacaacaa taacaataac aacaacctcg ggatcgaggg aaggatttca
     1381 gaatttgaaa acctgtattt tcagggccat atgaccggag cagcaactgc agcagaaaac
     1441 tctgccacac agttagaatt ctatagaaaa gctttgaatt tcaacgttat tgggagatac
     1501 gatccaaaaa taaagcaact actttttcac acaccacatg cgtcactgta taaatgggac
     1561 ttcaagaagg acgaatggaa taaactagaa tatcaaggtg ttttggccat atatttgaga
     1621 gacgtctcgc aaaatacaaa tcttctaccc gtctccccac aagaagtaga catttttgat
     1681 tcacaaaatg gtagtaataa cattcaagta aacaatggtt ctgacaatag caacaggaat
     1741 agcagtggaa atgggaactc ttataaaagt aatgattcgc taacatataa ttgcggcaaa
     1801 actttaagtg gaaaggacat atacaattat ggattgatca tattaaaccg aattaatcct
     1861 gacaattttt ctatgggcat cgttcccaat agtgtcgtaa ataaaagaaa agtatttaat
     1921 gcagaagagg atacacttaa cccactagag tgcatggggg tagaggtgaa agatgaattg
     1981 gtcattatta agaacttgaa ggctgaggtc tatggtatat ggattcatac agtcagtgat
     2041 agacaaaata tctacgaact aataaaatat cttctggaaa atgagccaaa agattctttt
     2101 gctggatccg ggtctggttc aggaatggcc atggtgagca agggcgagga gctgttcacc
     2161 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     2221 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     2281 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttgaccta cggcgtgcag
     2341 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     2401 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     2461 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     2521 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaag
     2581 gtctatatca ccgccgacaa gcagaagaac ggcatcaagg tgaacttcaa gacccgccac
     2641 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     2701 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccaa gctgagcaaa
     2761 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     2821 actctcggca tggacgagct gtacaaggga agcggccgcg aaaacctgta ttttcagggc
     2881 catcatcatc atcatcactg aaagcttggc actggccgtc gttttacaac gtcgtgactg
     2941 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     3001 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     3061 cgaatggcag cttggctgtt ttggcggatg agataagatt ttcagcctga tacagattaa
     3121 atcagaacgc agaagcggtc tgataaaaca gaatttgcct ggcggcagta gcgcggtggt
     3181 cccacctgac cccatgccga actcagaagt gaaacgccgt agcgccgatg gtagtgtggg
     3241 gtctccccat gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga
     3301 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa
     3361 atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg cgggcaggac
     3421 gcccgccata aactgccagg catcaaatta agcagaaggc catcctgacg gatggccttt
     3481 ttgcgtttct acaaactctt ttgtttattt ttctaaatac attcaaatat gtatccgctc
     3541 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     3601 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     3661 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     3721 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     3781 ttcccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     3841 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     3901 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     3961 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     4021 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     4081 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     4141 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     4201 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     4261 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     4321 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     4381 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     4441 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaccccgg
     4501 ttgataatca gaaaagcccc aaaaacagga agattgtata agcaaatatt taaattgtaa
     4561 acgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     4621 aataggccga aatcggcaaa atcccttata aatcaaaaga atagcccgag atagggttga
     4681 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     4741 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc aaatcaagtt
     4801 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     4861 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     4921 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     4981 cgcttaatgc gccgctacag ggcgcgtaaa aggatctagg tgaagatcct ttttgataat
     5041 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     5101 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     5161 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     5221 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     5281 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     5341 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     5401 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     5461 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     5521 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     5581 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     5641 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     5701 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     5761 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     5821 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     5881 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     5941 atgtggtgca ctctcagtac accgcatgtg gtgcactctc agtacaatct gctctgatgc
     6001 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc
     6061 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     6121 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     6181 ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgcag cgattcacag
     6241 atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg
     6301 cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc
     6361 gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc
     6421 acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa
     6481 ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc
     6541 gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg
     6601 aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg
     6661 aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt
     6721 cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg
     6781 gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgaa
     6841 attccgacac catcgaatgg tgcaaaacct ttcgcggtat ggcatgatag cgcccggaag
     6901 agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg
     6961 ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca ggccagccac gtttctgcga
     7021 aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg
     7081 cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc agtctggccc
     7141 tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca
     7201 gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca
     7261 atcttctcgc gcaacgcgtc agtgggctga tcattaacta tccgctggat gaccaggatg
     7321 ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt atttcttgat gtctctgacc
     7381 agacacccat caacagtatt attttctccc atgaagacgg tacgcgactg ggcgtggagc
     7441 atctggtcgc attgggtcac cagcaaatcg cgctgttagc gggcccatta agttctgtct
     7501 cggcgcgtct gcgtctggct ggctggcata aatatctcac tcgcaatcaa attcagccga
     7561 tagcggaacg ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc
     7621 tgaatgaggg catcgttccc actgcgatgc tggttgccaa cgatcagatg gcgctgggcg
     7681 caatgcgcgc cattaccgag tccgggctgc gcgttggtgc ggatatctcg gtagtgggat
     7741 acgacgatac cgaagacagc tcatgttata tcccgccgtt aaccaccatc aaacaggatt
     7801 ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg
     7861 tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca
     7921 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     7981 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtaagtta gctcactcat
     8041 taggcacaat tctcatgttt gacagcttat catcgactgc acggtgcacc aatgcttctg
     8101 gcgtcaggca gccatcggaa gctgtggtat ggctgtgcag gtcgtaaatc actgcat
//
