LOCUS       sequence-394533-        8013 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial protein expression of Pat1 241-422.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8013)
  AUTHORS   Currie SL, Xing W, Muhlrad D, Decker CJ, Parker R, Rosen MK
  TITLE     Quantitative reconstitution of yeast RNA processing bodies.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Apr 4;120(14):e2214064120. doi: 
            10.1073/pnas.2214064120. Epub 2023 Mar 27.
  PUBMED    36972455
REFERENCE   2  (bases 1 to 8013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-04-4"; volume: "120(14):e2214064120. 
            doi"; pages: " 10.1073/pnas.2214064120. Epub 2023 Mar 27"
COMMENT     Sequence Label: Pat1 241-422-EGFP
FEATURES             Location/Qualifiers
     source          1..8013
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(93..98,99..114,115..121)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    129..145
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     134..156
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             215..1315
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      1172..1181
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1289..1312
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1364..1375
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1388..1408
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(1988..1990,1991..1993,1994..2704)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2033..2054)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2294..2313)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2641..2662
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2717..2737
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2738..2755
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2768..2785)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2768..2784)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2777..2799)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2963..2980)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3133..3219
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3311..3338
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3357..3448
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3449..3517,3518..4309)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3667..3686)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(4351..4864)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4546..4567)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4758..4777
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      4975..5563
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5464..5483
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5717..5734
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5840..5859)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6000..6022
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6016..6207)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        6703..6780
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             6781..7863
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(6800..6819)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 taattcgtgt cgctcaaggc gcactcccgt tctggataat gttttttgcg ccgacatcat
       61 aacggttctg gcaaatattc tgaaatgagc tgttgacaat taatcatcgg ctcgtataat
      121 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agccagtccg tttaggtgtt
      181 ttcacgagca cttcaccaac aaggaccata gattatgaaa actgaagaag gtaaactggt
      241 aatctggatt aacggcgata aaggctataa cggtctcgct gaagtcggta agaaattcga
      301 gaaagatacc ggaattaaag tcaccgttga gcatccggat aaactggaag agaaattccc
      361 acaggttgcg gcaactggcg atggccctga cattatcttc tgggcacacg accgctttgg
      421 tggctacgct caatctggcc tgttggctga aatcaccccg gacaaagcgt tccaggacaa
      481 gctgtatccg tttacctggg atgccgtacg ttacaacggc aagctgattg cttacccgat
      541 cgctgttgaa gcgttatcgc tgatttataa caaagatctg ctgccgaacc cgccaaaaac
      601 ctgggaagag atcccggcgc tggataaaga actgaaagcg aaaggtaaga gcgcgctgat
      661 gttcaacctg caagaaccgt acttcacctg gccgctgatt gctgctgacg ggggttatgc
      721 gttcaagtat gaaaacggca agtacgacat taaagacgtg ggcgtggata acgctggcgc
      781 gaaagcgggt ctgaccttcc tggttgacct gattaaaaac aaacacatga atgcagacac
      841 cgattactcc atcgcagaag ctgcctttaa taaaggcgaa acagcgatga ccatcaacgg
      901 cccgtgggca tggtccaaca tcgacaccag caaagtgaat tatggtgtaa cggtactgcc
      961 gaccttcaag ggtcaaccat ccaaaccgtt cgttggcgtg ctgagcgcag gtattaacgc
     1021 cgccagtccg aacaaagagc tggcaaaaga gttcctcgaa aactatctgc tgactgatga
     1081 aggtctggaa gcggttaata aagacaaacc gctgggtgcc gtagcgctga agtcttacga
     1141 ggaagagttg gcgaaagatc cacgtattgc cgccaccatg gaaaacgccc agaaaggtga
     1201 aatcatgccg aacatcccgc agatgtccgc tttctggtat gccgtgcgta ctgcggtgat
     1261 caacgccgcc agcggtcgtc agactgtcga tgaagccctg aaagacgcgc agactaattc
     1321 gagctcgaac aacaacaaca ataacaataa caacaacctc gggatcgagg gaaggatttc
     1381 agaatttgaa aacctgtatt ttcagggcca tatgaaaatg cctctgcaaa gtggacgcag
     1441 atcgaagaga gatttgtcgc ctgaagagca aagacgtttg cagattcgtc atgccaaagt
     1501 ggagaaaatc ttgaaatact caggtttaat gactcctcgt gataaggact tcatcaccag
     1561 atatcagttg tctcaaattg tcactgagga cccttacaat gaggatttct acttccaggt
     1621 ctacaagatt atccaaagag gcggtatcac gtccgaatcc aacaaaggtt tgattgctag
     1681 ggcgtatttg gaacattctg gacacagact cggtggtcgc tataagagaa ccgatattgc
     1741 cctacagaga atgcaaagtc aagtagaaaa ggctgtcact gtggctaagg aaagaccttc
     1801 taagttgaag gatcaacaag cggctgctgg taactctagc caggataata agcaagcaaa
     1861 cacggttctg ggcaaaatct cttccacttt gaacagcaag aatccaagaa gacaactgca
     1921 gatccccaga caacagcctt ctgaccccga tgcgctaaaa ggatccgggt ctggttcagg
     1981 aatggccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     2041 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     2101 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     2161 gcccaccctc gtgaccacct tgacctacgg cgtgcagtgc ttcgcccgct accccgacca
     2221 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     2281 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     2341 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     2401 ggggcacaag ctggagtaca actacaacag ccacaaggtc tatatcaccg ccgacaagca
     2461 gaagaacggc atcaaggtga acttcaagac ccgccacaac atcgaggacg gcagcgtgca
     2521 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     2581 caaccactac ctgagcaccc agtccaagct gagcaaagac cccaacgaga agcgcgatca
     2641 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     2701 caagggaagc ggccgcgaaa acctgtattt tcagggccat catcatcatc atcactgaaa
     2761 gcttggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     2821 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     2881 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcagctt ggctgttttg
     2941 gcggatgaga taagattttc agcctgatac agattaaatc agaacgcaga agcggtctga
     3001 taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact
     3061 cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga
     3121 actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     3181 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac
     3241 gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat
     3301 caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg
     3361 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     3421 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     3481 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     3541 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     3601 cggtaagatc cttgagagtt ttcgccccga agaacgtttc ccaatgatga gcacttttaa
     3661 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
     3721 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     3781 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     3841 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     3901 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     3961 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     4021 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     4081 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     4141 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     4201 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     4261 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     4321 agtttactca tatatacttt agattgattt accccggttg ataatcagaa aagccccaaa
     4381 aacaggaaga ttgtataagc aaatatttaa attgtaaacg ttaatatttt gttaaaattc
     4441 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     4501 ccttataaat caaaagaata gcccgagata gggttgagtg ttgttccagt ttggaacaag
     4561 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     4621 gatggcccac tacgtgaacc atcacccaaa tcaagttttt tggggtcgag gtgccgtaaa
     4681 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     4741 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     4801 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     4861 gcgtaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     4921 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     4981 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     5041 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     5101 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     5161 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     5221 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     5281 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     5341 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     5401 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     5461 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     5521 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     5581 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     5641 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     5701 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
     5761 ttttctcctt acgcatctgt gcggtatttc acaccgcatg tggtgcactc tcagtacacc
     5821 gcatgtggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
     5881 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
     5941 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     6001 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg
     6061 gtaaagctca tcagcgtggt cgtgcagcga ttcacagatg tctgcctgtt catccgcgtc
     6121 cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt
     6181 aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat
     6241 gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga
     6301 acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga
     6361 ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc
     6421 acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga
     6481 cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca
     6541 ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt
     6601 ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat
     6661 catgcgcacc cgtggccagg acccaacgct gcccgaaatt ccgacaccat cgaatggtgc
     6721 aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat
     6781 gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt
     6841 tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg
     6901 gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag
     6961 tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc
     7021 gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa
     7081 cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt
     7141 gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc
     7201 actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt
     7261 ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag
     7321 caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc
     7381 tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg
     7441 agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact
     7501 gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc
     7561 gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca
     7621 tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc
     7681 gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc
     7741 gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc
     7801 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     7861 tgagcgcaac gcaattaatg taagttagct cactcattag gcacaattct catgtttgac
     7921 agcttatcat cgactgcacg gtgcaccaat gcttctggcg tcaggcagcc atcggaagct
     7981 gtggtatggc tgtgcaggtc gtaaatcact gca
//
