LOCUS       sequence-394535-        8184 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial protein expression of Pat1 1-240.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8184)
  AUTHORS   Currie SL, Xing W, Muhlrad D, Decker CJ, Parker R, Rosen MK
  TITLE     Quantitative reconstitution of yeast RNA processing bodies.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Apr 4;120(14):e2214064120. doi: 
            10.1073/pnas.2214064120. Epub 2023 Mar 27.
  PUBMED    36972455
REFERENCE   2  (bases 1 to 8184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-04-4"; volume: "120(14):e2214064120. 
            doi"; pages: " 10.1073/pnas.2214064120. Epub 2023 Mar 27"
COMMENT     Sequence Label: Pat1 1-240-EGFP
FEATURES             Location/Qualifiers
     source          1..8184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(82..87,88..103,104..110)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    118..134
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     123..145
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             204..1304
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      1161..1170
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1278..1301
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1353..1364
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1377..1397
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(2148..2150,2151..2153,2154..2864)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2193..2214)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2454..2473)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2801..2822
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2877..2897
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2898..2915
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2928..2945)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2928..2944)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2937..2959)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3123..3140)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3293..3379
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3471..3498
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3517..3608
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3609..3677,3678..4469)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3827..3846)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(4511..5024)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4706..4727)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4918..4937
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      5135..5723
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5624..5643
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5877..5894
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6000..6019)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6160..6182
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6176..6367)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        6863..6940
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             6941..8023
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(6960..6979)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 gctcaaggcg cactcccgtt ctggataatg ttttttgcgc cgacatcata acggttctgg
       61 caaatattct gaaatgagct gttgacaatt aatcatcggc tcgtataatg tgtggaattg
      121 tgagcggata acaatttcac acaggaaaca gccagtccgt ttaggtgttt tcacgagcac
      181 ttcaccaaca aggaccatag attatgaaaa ctgaagaagg taaactggta atctggatta
      241 acggcgataa aggctataac ggtctcgctg aagtcggtaa gaaattcgag aaagataccg
      301 gaattaaagt caccgttgag catccggata aactggaaga gaaattccca caggttgcgg
      361 caactggcga tggccctgac attatcttct gggcacacga ccgctttggt ggctacgctc
      421 aatctggcct gttggctgaa atcaccccgg acaaagcgtt ccaggacaag ctgtatccgt
      481 ttacctggga tgccgtacgt tacaacggca agctgattgc ttacccgatc gctgttgaag
      541 cgttatcgct gatttataac aaagatctgc tgccgaaccc gccaaaaacc tgggaagaga
      601 tcccggcgct ggataaagaa ctgaaagcga aaggtaagag cgcgctgatg ttcaacctgc
      661 aagaaccgta cttcacctgg ccgctgattg ctgctgacgg gggttatgcg ttcaagtatg
      721 aaaacggcaa gtacgacatt aaagacgtgg gcgtggataa cgctggcgcg aaagcgggtc
      781 tgaccttcct ggttgacctg attaaaaaca aacacatgaa tgcagacacc gattactcca
      841 tcgcagaagc tgcctttaat aaaggcgaaa cagcgatgac catcaacggc ccgtgggcat
      901 ggtccaacat cgacaccagc aaagtgaatt atggtgtaac ggtactgccg accttcaagg
      961 gtcaaccatc caaaccgttc gttggcgtgc tgagcgcagg tattaacgcc gccagtccga
     1021 acaaagagct ggcaaaagag ttcctcgaaa actatctgct gactgatgaa ggtctggaag
     1081 cggttaataa agacaaaccg ctgggtgccg tagcgctgaa gtcttacgag gaagagttgg
     1141 cgaaagatcc acgtattgcc gccaccatgg aaaacgccca gaaaggtgaa atcatgccga
     1201 acatcccgca gatgtccgct ttctggtatg ccgtgcgtac tgcggtgatc aacgccgcca
     1261 gcggtcgtca gactgtcgat gaagccctga aagacgcgca gactaattcg agctcgaaca
     1321 acaacaacaa taacaataac aacaacctcg ggatcgaggg aaggatttca gaatttgaaa
     1381 acctgtattt tcagggccat atgtccttct ttgggttaga aaatagcggt aatgcgcggg
     1441 atggtcctct ggactttgaa gagagttaca agggctatgg cgagcacgaa cttgaggaga
     1501 acgactattt gaacgacgaa acatttggtg ataatgttca ggttggtacc gactttgatt
     1561 ttggaaatcc tcacagcagc ggcagcagcg gcaacgcaat tggtggtaat ggcgtcggtg
     1621 ccacggctag atcgtatgtt gcagctactg cagaaggaat tagcggccct aggaccgatg
     1681 gaacggcagc agcaggacct ctagacctga agccaatgga atctttgtgg tctactgcac
     1741 cacctccagc aatggcgcct tcaccccaaa gtacaatggc tccggctcct gctccgcagc
     1801 aaatggcccc cctacagcca atcttgtcga tgcaagactt ggaaagacaa caacgtcaaa
     1861 tgcagcaaca gtttatgaat ttccacgcca tgggtcatcc acagggtctc ccacagggtc
     1921 cgcctcagca gcaatttcca atgcagcctg cgtcgggtca accaggtccc tcacaatttg
     1981 cgcctccacc tccacctcct ggcgttaatg tgaatatgaa tcaaatgcca atgggtcctg
     2041 tacaagttcc agttcaagct tcgccttcac ccatcggtat gtccaacact ccttctccag
     2101 gccctgtggt tggcgcaact ggatccgggt ctggttcagg aatggccatg gtgagcaagg
     2161 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     2221 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     2281 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct
     2341 tgacctacgg cgtgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct
     2401 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     2461 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     2521 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     2581 actacaacag ccacaaggtc tatatcaccg ccgacaagca gaagaacggc atcaaggtga
     2641 acttcaagac ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     2701 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     2761 agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     2821 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagggaagc ggccgcgaaa
     2881 acctgtattt tcagggccat catcatcatc atcactgaaa gcttggcact ggccgtcgtt
     2941 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     3001 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     3061 ttgcgcagcc tgaatggcga atggcagctt ggctgttttg gcggatgaga taagattttc
     3121 agcctgatac agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc
     3181 ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc
     3241 gccgatggta gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa
     3301 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     3361 tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg
     3421 agggtggcgg gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat
     3481 cctgacggat ggcctttttg cgtttctaca aactcttttg tttatttttc taaatacatt
     3541 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     3601 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     3661 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     3721 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     3781 ttcgccccga agaacgtttc ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     3841 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     3901 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     3961 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     4021 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     4081 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     4141 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     4201 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     4261 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     4321 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     4381 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     4441 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     4501 agattgattt accccggttg ataatcagaa aagccccaaa aacaggaaga ttgtataagc
     4561 aaatatttaa attgtaaacg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     4621 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     4681 gcccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     4741 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     4801 atcacccaaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     4861 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     4921 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     4981 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtaaaagg atctaggtga
     5041 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     5101 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     5161 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     5221 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     5281 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     5341 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     5401 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     5461 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     5521 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     5581 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     5641 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     5701 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     5761 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     5821 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     5881 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt
     5941 gcggtatttc acaccgcatg tggtgcactc tcagtacacc gcatgtggtg cactctcagt
     6001 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
     6061 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     6121 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     6181 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     6241 cgtgcagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     6301 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     6361 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     6421 aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac
     6481 gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg
     6541 gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc
     6601 ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt
     6661 acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc
     6721 agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac
     6781 cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg
     6841 acccaacgct gcccgaaatt ccgacaccat cgaatggtgc aaaacctttc gcggtatggc
     6901 atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgttata
     6961 cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc
     7021 cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta
     7081 cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc
     7141 cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc
     7201 cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg
     7261 taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc
     7321 gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt
     7381 tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac
     7441 gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg
     7501 cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg
     7561 caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca
     7621 acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga
     7681 tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga
     7741 tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac
     7801 caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact
     7861 ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa
     7921 aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     7981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     8041 taagttagct cactcattag gcacaattct catgtttgac agcttatcat cgactgcacg
     8101 gtgcaccaat gcttctggcg tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc
     8161 gtaaatcact gcataattcg tgtc
//
