LOCUS       sequence-394544-        8013 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial protein expression of Dcp1 del82-129.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8013)
  AUTHORS   Currie SL, Xing W, Muhlrad D, Decker CJ, Parker R, Rosen MK
  TITLE     Quantitative reconstitution of yeast RNA processing bodies.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Apr 4;120(14):e2214064120. doi: 
            10.1073/pnas.2214064120. Epub 2023 Mar 27.
  PUBMED    36972455
REFERENCE   2  (bases 1 to 8013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-04-4"; volume: "120(14):e2214064120. 
            doi"; pages: " 10.1073/pnas.2214064120. Epub 2023 Mar 27"
COMMENT     Sequence Label: Dcp1 D82-129-EGFP
FEATURES             Location/Qualifiers
     source          1..8013
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(108..113,114..129,130..136)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    144..160
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     149..171
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             230..1330
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      1187..1196
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1304..1327
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1379..1390
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1403..1423
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(2003..2005,2006..2008,2009..2719)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2048..2069)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2309..2328)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2656..2677
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2732..2752
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2753..2770
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2783..2800)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2783..2799)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2792..2814)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2978..2995)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3148..3234
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3326..3353
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3372..3463
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3464..3532,3533..4324)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3682..3701)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(4366..4879)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4561..4582)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4773..4792
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      4990..5578
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5479..5498
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5732..5749
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5855..5874)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6015..6037
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6031..6222)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        6718..6795
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             6796..7878
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(6815..6834)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 tcgtaaatca ctgcataatt cgtgtcgctc aaggcgcact cccgttctgg ataatgtttt
       61 ttgcgccgac atcataacgg ttctggcaaa tattctgaaa tgagctgttg acaattaatc
      121 atcggctcgt ataatgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcca
      181 gtccgtttag gtgttttcac gagcacttca ccaacaagga ccatagatta tgaaaactga
      241 agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt
      301 cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact
      361 ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc
      421 acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa
      481 agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct
      541 gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc
      601 gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg
      661 taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc
      721 tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt
      781 ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca
      841 catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc
      901 gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg
      961 tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag
     1021 cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta
     1081 tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc
     1141 gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ccatggaaaa
     1201 cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt
     1261 gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga
     1321 cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat
     1381 cgagggaagg atttcagaat ttgaaaacct gtattttcag ggccatatga ccggagcagc
     1441 aactgcagca gaaaactctg ccacacagtt agaattctat agaaaagctt tgaatttcaa
     1501 cgttattggg agatacgatc caaaaataaa gcaactactt tttcacacac cacatgcgtc
     1561 actgtataaa tgggacttca agaaggacga atggaataaa ctagaatatc aaggtgtttt
     1621 ggccatatat ttgagagacg tctcgcaaaa tacaaatctt ctacccgtca aaactttaag
     1681 tggaaaggac atatacaatt atggattgat catattaaac cgaattaatc ctgacaattt
     1741 ttctatgggc atcgttccca atagtgtcgt aaataaaaga aaagtattta atgcagaaga
     1801 ggatacactt aacccactag agtgcatggg ggtagaggtg aaagatgaat tggtcattat
     1861 taagaacttg aagcatgagg tctatggtat atggattcat acagtcagtg atagacaaaa
     1921 tatctacgaa ctaataaaat atcttctgga aaatgagcca aaagattctt ttgctggatc
     1981 cgggtctggt tcaggaatgg ccatggtgag caagggcgag gagctgttca ccggggtggt
     2041 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     2101 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     2161 gctgcccgtg ccctggccca ccctcgtgac caccttgacc tacggcgtgc agtgcttcgc
     2221 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     2281 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     2341 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     2401 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca aggtctatat
     2461 caccgccgac aagcagaaga acggcatcaa ggtgaacttc aagacccgcc acaacatcga
     2521 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     2581 cgtgctgctg cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa
     2641 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     2701 catggacgag ctgtacaagg gaagcggccg cgaaaacctg tattttcagg gccatcatca
     2761 tcatcatcac tgaaagcttg gcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     2821 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     2881 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
     2941 agcttggctg ttttggcgga tgagataaga ttttcagcct gatacagatt aaatcagaac
     3001 gcagaagcgg tctgataaaa cagaatttgc ctggcggcag tagcgcggtg gtcccacctg
     3061 accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg gggtctcccc
     3121 atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     3181 gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg
     3241 ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca
     3301 taaactgcca ggcatcaaat taagcagaag gccatcctga cggatggcct ttttgcgttt
     3361 ctacaaactc ttttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     3421 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     3481 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     3541 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     3601 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gtttcccaat
     3661 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca
     3721 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     3781 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     3841 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     3901 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     3961 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     4021 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     4081 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     4141 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     4201 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     4261 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     4321 gtaactgtca gaccaagttt actcatatat actttagatt gatttacccc ggttgataat
     4381 cagaaaagcc ccaaaaacag gaagattgta taagcaaata tttaaattgt aaacgttaat
     4441 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     4501 gaaatcggca aaatccctta taaatcaaaa gaatagcccg agatagggtt gagtgttgtt
     4561 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     4621 accgtctatc agggcgatgg cccactacgt gaaccatcac ccaaatcaag ttttttgggg
     4681 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     4741 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     4801 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     4861 gcgccgctac agggcgcgta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     4921 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     4981 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     5041 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     5101 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     5161 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     5221 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     5281 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     5341 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     5401 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     5461 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     5521 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     5581 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     5641 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     5701 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     5761 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatgtggtg
     5821 cactctcagt acaccgcatg tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     5881 taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc
     5941 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     6001 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     6061 cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtgc agcgattcac agatgtctgc
     6121 ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat
     6181 aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg
     6241 gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg
     6301 ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt
     6361 atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac
     6421 agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat
     6481 ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat
     6541 tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg
     6601 tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa
     6661 cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg aaattccgac
     6721 accatcgaat ggtgcaaaac ctttcgcggt atggcatgat agcgcccgga agagagtcaa
     6781 ttcagggtgg tgaatgtgaa accagtaacg ttatacgatg tcgcagagta tgccggtgtc
     6841 tcttatcaga ccgtttcccg cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg
     6901 gaaaaagtgg aagcggcgat ggcggagctg aattacattc ccaaccgcgt ggcacaacaa
     6961 ctggcgggca aacagtcgtt gctgattggc gttgccacct ccagtctggc cctgcacgcg
     7021 ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc cagcgtggtg
     7081 gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc
     7141 gcgcaacgcg tcagtgggct gatcattaac tatccgctgg atgaccagga tgccattgct
     7201 gtggaagctg cctgcactaa tgttccggcg ttatttcttg atgtctctga ccagacaccc
     7261 atcaacagta ttattttctc ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc
     7321 gcattgggtc accagcaaat cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt
     7381 ctgcgtctgg ctggctggca taaatatctc actcgcaatc aaattcagcc gatagcggaa
     7441 cgggaaggcg actggagtgc catgtccggt tttcaacaaa ccatgcaaat gctgaatgag
     7501 ggcatcgttc ccactgcgat gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc
     7561 gccattaccg agtccgggct gcgcgttggt gcggatatct cggtagtggg atacgacgat
     7621 accgaagaca gctcatgtta tatcccgccg ttaaccacca tcaaacagga ttttcgcctg
     7681 ctggggcaaa ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc
     7741 aatcagctgt tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc caatacgcaa
     7801 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     7861 ctggaaagcg ggcagtgagc gcaacgcaat taatgtaagt tagctcactc attaggcaca
     7921 attctcatgt ttgacagctt atcatcgact gcacggtgca ccaatgcttc tggcgtcagg
     7981 cagccatcgg aagctgtggt atggctgtgc agg
//
