LOCUS       sequence-394545-        8388 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial protein expression of Edc3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8388)
  AUTHORS   Currie SL, Xing W, Muhlrad D, Decker CJ, Parker R, Rosen MK
  TITLE     Quantitative reconstitution of yeast RNA processing bodies.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Apr 4;120(14):e2214064120. doi: 
            10.1073/pnas.2214064120. Epub 2023 Mar 27.
  PUBMED    36972455
REFERENCE   2  (bases 1 to 8388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-04-4"; volume: "120(14):e2214064120. 
            doi"; pages: " 10.1073/pnas.2214064120. Epub 2023 Mar 27"
COMMENT     Sequence Label: Edc3
FEATURES             Location/Qualifiers
     source          1..8388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(81..86,87..102,103..109)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    117..133
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     122..144
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             203..1303
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      1160..1169
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1277..1300
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1352..1363
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1376..1396
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3053..3070
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3080..3100
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3101..3118
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3131..3148)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3131..3147)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3140..3162)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3326..3343)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3496..3582
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3674..3701
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3720..3811
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3812..3880,3881..4672)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4030..4049)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(4714..5227)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4909..4930)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5121..5140
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      5338..5926
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5827..5846
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6080..6097
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6203..6222)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6363..6385
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6379..6570)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        7066..7143
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             7144..8226
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(7163..7182)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 ctcaaggcgc actcccgttc tggataatgt tttttgcgcc gacatcataa cggttctggc
       61 aaatattctg aaatgagctg ttgacaatta atcatcggct cgtataatgt gtggaattgt
      121 gagcggataa caatttcaca caggaaacag ccagtccgtt taggtgtttt cacgagcact
      181 tcaccaacaa ggaccataga ttatgaaaac tgaagaaggt aaactggtaa tctggattaa
      241 cggcgataaa ggctataacg gtctcgctga agtcggtaag aaattcgaga aagataccgg
      301 aattaaagtc accgttgagc atccggataa actggaagag aaattcccac aggttgcggc
      361 aactggcgat ggccctgaca ttatcttctg ggcacacgac cgctttggtg gctacgctca
      421 atctggcctg ttggctgaaa tcaccccgga caaagcgttc caggacaagc tgtatccgtt
      481 tacctgggat gccgtacgtt acaacggcaa gctgattgct tacccgatcg ctgttgaagc
      541 gttatcgctg atttataaca aagatctgct gccgaacccg ccaaaaacct gggaagagat
      601 cccggcgctg gataaagaac tgaaagcgaa aggtaagagc gcgctgatgt tcaacctgca
      661 agaaccgtac ttcacctggc cgctgattgc tgctgacggg ggttatgcgt tcaagtatga
      721 aaacggcaag tacgacatta aagacgtggg cgtggataac gctggcgcga aagcgggtct
      781 gaccttcctg gttgacctga ttaaaaacaa acacatgaat gcagacaccg attactccat
      841 cgcagaagct gcctttaata aaggcgaaac agcgatgacc atcaacggcc cgtgggcatg
      901 gtccaacatc gacaccagca aagtgaatta tggtgtaacg gtactgccga ccttcaaggg
      961 tcaaccatcc aaaccgttcg ttggcgtgct gagcgcaggt attaacgccg ccagtccgaa
     1021 caaagagctg gcaaaagagt tcctcgaaaa ctatctgctg actgatgaag gtctggaagc
     1081 ggttaataaa gacaaaccgc tgggtgccgt agcgctgaag tcttacgagg aagagttggc
     1141 gaaagatcca cgtattgccg ccaccatgga aaacgcccag aaaggtgaaa tcatgccgaa
     1201 catcccgcag atgtccgctt tctggtatgc cgtgcgtact gcggtgatca acgccgccag
     1261 cggtcgtcag actgtcgatg aagccctgaa agacgcgcag actaattcga gctcgaacaa
     1321 caacaacaat aacaataaca acaacctcgg gatcgaggga aggatttcag aatttgaaaa
     1381 cctgtatttt cagggccata tgtcacaatt tgttggtttc ggagtacaag tggagctaaa
     1441 agatgggaag ctcattcagg ggaaaattgc caaagcaacc tcaaaaggat tgactttaaa
     1501 tgacgttcaa ttcggcgatg gtggtaaatc tcaagctttc aaagtgaggg cgtcaaggct
     1561 aaaggactta aaggttctaa ctgttgcctc tcaatccggg aaaaggaagc agcaaagaca
     1621 acagcagcaa caaaacgatt ataatcaaaa tcgcggtgaa catattgatt ggcaagatga
     1681 tgatgttagt aagataaaac aacaggaaga tttcgatttc caaagaaatt tgggcatgtt
     1741 taacaaaaaa gacgtcttcg cacaattaaa gcaaaatgac gatatattac cggagaatag
     1801 attacaggga cataacagaa agcaaaccca attgcagcaa aataattatc aaaatgatga
     1861 attggttatt ccagatgcaa agaaagattc atggaacaag atttcttcaa gaaatgagca
     1921 aagcacacac caatctcagc cgcaacaaga tgctcaagat gatctggttt tggaagatga
     1981 tgaacatgaa tacgatgtcg atgatatcga tgatcccaaa tacctaccaa taactcagtc
     2041 tttgaatatt acacatttaa ttcactctgc aactaactct ccatccataa atgataaaac
     2101 gaaaggtaca gttataaatg ataaggatca ggtactagct aaattaggcc agatgatcat
     2161 cagccagtca agatccaact caacatcctt gccagctgca aataaacaaa caaccatcag
     2221 atcaaagaac actaagcaga acattcctat ggctacacca gtacaactac tagaaatgga
     2281 gagcatcacg tccgaatttt tcagtattaa ctcggcaggg ctactagaaa attttgctgt
     2341 aaacgcatcg ttcttcttaa agcagaaact aggtggccgt gcacgtttac gtttacaaaa
     2401 ttctaatccg gaacctttag tagtaatact agcctcagat tccaacagat ctggtgcgaa
     2461 agctctggcg ttgggtagac atctttgcca aacggggcat atccgtgtca taacattatt
     2521 tacatgttct caaaatgaac tacaggattc catggtcaaa aagcaaacag atatttacaa
     2581 gaagtgtggc ggaaaaattg ttaatagtgt atcgtcgctg gaatctgcta tggaaacatt
     2641 aaatagccct gtagaaatag tcatcgatgc catgcaggga tatgactgta cattgagcga
     2701 tctggcgggg acgtcggaag ttattgaaag cagaattaaa agcatgatat catggtgtaa
     2761 caaacagcga ggatctacta aagtgtggtc tttggatatt ccaaatgggt ttgatgcggg
     2821 ctcgggcatg ccagatattt tcttttcaga caggattgaa gcaacaggaa ttatttgttc
     2881 tggctggcct ttgattgcca tcaacaactt aattgcaaat ttgccaagtc tagaagatgc
     2941 tgttttgatt gatataggta taccacaggg cgcctattca cagagaactt ctttgcgtaa
     3001 gttccaaaac tgtgatcttt tcgtcactga cgggtccctg ctattagatt tgcaccatca
     3061 ccatcatcat tagggatccg aaaacctgta ttttcagggc catcatcatc atcatcactg
     3121 aaagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     3181 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     3241 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcag cttggctgtt
     3301 ttggcggatg agataagatt ttcagcctga tacagattaa atcagaacgc agaagcggtc
     3361 tgataaaaca gaatttgcct ggcggcagta gcgcggtggt cccacctgac cccatgccga
     3421 actcagaagt gaaacgccgt agcgccgatg gtagtgtggg gtctccccat gcgagagtag
     3481 ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     3541 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg
     3601 aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg
     3661 catcaaatta agcagaaggc catcctgacg gatggccttt ttgcgtttct acaaactctt
     3721 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     3781 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     3841 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     3901 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     3961 cagcggtaag atccttgaga gttttcgccc cgaagaacgt ttcccaatga tgagcacttt
     4021 taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg
     4081 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     4141 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     4201 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     4261 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     4321 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     4381 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     4441 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     4501 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     4561 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     4621 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     4681 ccaagtttac tcatatatac tttagattga tttaccccgg ttgataatca gaaaagcccc
     4741 aaaaacagga agattgtata agcaaatatt taaattgtaa acgttaatat tttgttaaaa
     4801 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     4861 atcccttata aatcaaaaga atagcccgag atagggttga gtgttgttcc agtttggaac
     4921 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     4981 ggcgatggcc cactacgtga accatcaccc aaatcaagtt ttttggggtc gaggtgccgt
     5041 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     5101 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     5161 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     5221 ggcgcgtaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     5281 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     5341 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     5401 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     5461 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     5521 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     5581 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     5641 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     5701 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     5761 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     5821 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     5881 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     5941 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     6001 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     6061 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     6121 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atgtggtgca ctctcagtac
     6181 accgcatgtg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
     6241 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
     6301 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     6361 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct
     6421 gcggtaaagc tcatcagcgt ggtcgtgcag cgattcacag atgtctgcct gttcatccgc
     6481 gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat
     6541 gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt
     6601 catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga
     6661 tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg
     6721 ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt
     6781 tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc
     6841 tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc
     6901 tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc
     6961 attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac
     7021 gatcatgcgc acccgtggcc aggacccaac gctgcccgaa attccgacac catcgaatgg
     7081 tgcaaaacct ttcgcggtat ggcatgatag cgcccggaag agagtcaatt cagggtggtg
     7141 aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc
     7201 gtttcccgcg tggtgaacca ggccagccac gtttctgcga aaacgcggga aaaagtggaa
     7261 gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa
     7321 cagtcgttgc tgattggcgt tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt
     7381 gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta
     7441 gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc
     7501 agtgggctga tcattaacta tccgctggat gaccaggatg ccattgctgt ggaagctgcc
     7561 tgcactaatg ttccggcgtt atttcttgat gtctctgacc agacacccat caacagtatt
     7621 attttctccc atgaagacgg tacgcgactg ggcgtggagc atctggtcgc attgggtcac
     7681 cagcaaatcg cgctgttagc gggcccatta agttctgtct cggcgcgtct gcgtctggct
     7741 ggctggcata aatatctcac tcgcaatcaa attcagccga tagcggaacg ggaaggcgac
     7801 tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc
     7861 actgcgatgc tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc cattaccgag
     7921 tccgggctgc gcgttggtgc ggatatctcg gtagtgggat acgacgatac cgaagacagc
     7981 tcatgttata tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc
     8041 agcgtggacc gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg
     8101 cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc
     8161 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     8221 cagtgagcgc aacgcaatta atgtaagtta gctcactcat taggcacaat tctcatgttt
     8281 gacagcttat catcgactgc acggtgcacc aatgcttctg gcgtcaggca gccatcggaa
     8341 gctgtggtat ggctgtgcag gtcgtaaatc actgcataat tcgtgtcg
//
