LOCUS       sequence-394547-        9560 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cre reporter for mammalian cells with Chrm3 membrane anchor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9560)
  AUTHORS   Strecker J, Demircioglu FE, Li D, Faure G, Wilkinson ME, Gootenberg 
            JS, Abudayyeh OO, Nishimasu H, Macrae RK, Zhang F
  TITLE     RNA-activated protein cleavage with a CRISPR-associated 
            endopeptidase.
  JOURNAL   Science. 2022 Nov 25;378(6622):874-881. doi: 
            10.1126/science.add7450. Epub 2022 Nov 3.
  PUBMED    36423276
REFERENCE   2  (bases 1 to 9560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.add7450"; journalName: "Science"; date: 
            "2022-11-25- 25"; volume: "378"; issue: "6622"; pages: "874-881"
COMMENT     Sequence Label: pCDNA3_Chrm3_Csx30_Cre
FEATURES             Location/Qualifiers
     source          1..9560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        170..418
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     170..190
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     341..360
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        538..917
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        918..1121
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1072..1092
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1166..1185
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1166..1184
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3008..3017
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3968..4048
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             4052..5080
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     primer_bind     complement(5151..5168)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5157..5381
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5427..5855
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5514..5533)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5724..5745
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5864..5884)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5869..6198
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6049..6184
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6111..6130
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6265..7059
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6319..6338)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6929..6948
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7233..7354
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7270..7289)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7324..7343
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7403..7419)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7403..7419)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7416..7438)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7427..7443
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7451..7457,7458..7475,7476..7481))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7496..7517
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7634..7651)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7805..8393)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7885..7904)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8564..9355,9356..9424))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9187..9206
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9425..9529)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga cttaattaag agggcctatt
      181 tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattggaat
      241 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt
      301 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt
      361 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgt
      421 aatttctact cttgtagatg gagacgatat atcgtctctt tttcaattgc atgaagaatc
      481 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      541 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      601 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      661 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      721 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      781 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      841 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      901 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      961 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     1021 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     1081 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac
     1141 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct
     1201 agcgtttaaa cttaagcttg ccaccatgac cctccacaac aatagcacca caagccccct
     1261 gtttcccaac atcagcagct cctggatcca ctcgccttct gatgccggcc tgccacctgg
     1321 caccgtgaca cacttcggct cttacaacgt gtccagagcc gccggaaact tcagctcccc
     1381 agatggcacc acagacgacc ctctgggcgg ccacaccgtg tggcaggttg tgttcatcgc
     1441 ctttctgacg ggcatcctgg ccctggtgac catcatcggc aatatcctgg ttatcgtgtc
     1501 ctttaaggtg aacaagcagc tgaagaccgt gaacaactac ttcctgctga gcctggcctg
     1561 tgccgacctg atcatcggag tgatcagcat gaacctgttc acaacctaca tcatcatgaa
     1621 ccggtgggcc ctgggcaacc tggcttgtga cctgtggctg gccatcgact acgtggccag
     1681 caacgccagc gtgatgaacc tgctggtgat ctctttcgac agatacttca gcatcacccg
     1741 gcctctgaca tacagagcca agaggacaac caagagagcc ggcgtgatga tcggactggc
     1801 ttgggtcatt agcttcgtgc tgtgggcccc tgccatcctg ttttggcagt atttcgtggg
     1861 caaacggaca gttcctcctg gagagtgctt catccagttc ctgtccgagc ctacaattac
     1921 ctttggaact gccattgccg ccttctacat gcctgtgaca atcatgacaa tcctgtactg
     1981 gcggatctac aaggaaacag agaaaagaac caaagaactg gctggcctgc aggcctctgg
     2041 cacagaagcc gagacagaga atttcgtgca ccctaccggt agcagcagat catgcagcag
     2101 ctacgagcta cagcaacagt ccatgaaacg ttctaataga cggaaatacg gcagatgcca
     2161 cttttggttc accacaaagt cctggaagcc tagcagcgaa cagatggacc aggaccactc
     2221 tagctctgat agctggaaca acaacgacgc cgctgccagt ctggaaaaca gcgccagctc
     2281 tgatgaggaa gatatcggca gcgaaaccag agccatttat tctatcgtgc tgaagctgcc
     2341 tggccatagc accatcctga acagcaccaa gctgccctct agcgacaacc tccaggtgcc
     2401 cgaggaagag ctgggcatgg tggacctgga aagaaaggcc gataagctgc aagcccagaa
     2461 gagcgtggat gacggcggca gcttccctaa gtccttcagc aagctgccaa tccaactgga
     2521 gagcgccgtg gacaccgcca agaccagcga tgtgaacagc tcggtcggca agagcacagc
     2581 aaccctgccc ctgtccttca aagaggccac cctggccaag cggttcgccc tgaaaacaag
     2641 atcacagatc accaagcgga agagaatgag cctggtcaag gagaagaagg ccgctcagac
     2701 ccttagcgct atcctgctcg ctttcatcat cacctggacc ccttacaaca tcatggtcct
     2761 ggtgaatacc ttctgcgaca gctgtatccc caagaccttc tggaatctgg gctactggct
     2821 gtgctacatc aactccaccg tgaaccccgt gtgctacgcc ctgtgcaaca agacattcag
     2881 aaccaccttt aagatgctgc tgctgtgtca gtgcgacaag aaaaagagaa gaaagcagca
     2941 gtaccagcaa cggcagagcg tgatcttcca caagcgcgct cctgagcagg ccttgggcgg
     3001 agggtccgcc accatgggct ataatcaccc gtatccggct gatgtgggta tgcgtattca
     3061 tcaggttgcc cgcgcggttt tttcgcaaac gaacctgtcc ccggcggaac gcctggctgt
     3121 agccatcgct ggagcgtgct tcaccccgga aatcagcgag gaccgtcgtt tggagatcct
     3181 cttggactgt gaagaacgtg tctgtgaaat tgaagcaccg actggtgacg acacgagcgt
     3241 acgtgttatc aaggatctga aggccctggc tgatcatcgt gtccgccacg aaatcccagc
     3301 cgagtccctc gtgagcctgt ggtttgaaca gattgaagcc gcaggcacgg acttcgatac
     3361 caaaacccct atggatgagc ttgtgctgcg tatgctgtcc gataacgtga tcaccctgtc
     3421 ggtggatcgt aaagccgcga gccaaaccga gacagacgac gttaagccgc agaaaggtaa
     3481 aatcatcccg ttcccggttc cggatatcgc gaacgatgag gtggagtacc agaaagctgt
     3541 gggtatgaaa aaggacaaaa aggccgcgaa cgactccaaa gtgaagtttc cgggtctgtt
     3601 ggagatccaa ggttgccgtg atggcgataa ggcgattcta ttggaggaca ctgacgacgc
     3661 agccgcgaac caccgcaaac tatttagtat tctgaaggcg ggtaagctga acagcgcgtt
     3721 cttcatccaa tctgacgacg gcgagtgggt tgaatctgag agtaagccga ccatggaaga
     3781 caatcgtatt attttgcacg atagccacca cagcagcttc gtttggattc tcgacacggg
     3841 tagcatgcag ctgcgccaaa gcgttaagtg cgttaaggat gcgctgaata aaaagacggg
     3901 ctctgcgaaa aaattgaagc caaaaaccat gatcgtgtgg gtcaccattc cgcaagaagg
     3961 cggatcttac ccatacgatg ttccagatta cgcttatccc tacgacgtgc ctgattatgc
     4021 atacccatat gatgtccccg actatgccgc gagcaattta ctgaccgtac accaaaattt
     4081 gcctgcatta ccggtcgatg caacgagtga tgaggttcgc aagaacctga tggacatgtt
     4141 cagggatcgc caggcgtttt ctgagcatac ctggaaaatg cttctgtccg tttgccggtc
     4201 gtgggcggca tggtgcaagt tgaataaccg gaaatggttt cccgcagaac ctgaagatgt
     4261 tcgcgattat cttctatatc ttcaggcgcg cggtctggca gtaaaaacta tccagcaaca
     4321 tttgggccag ctaaacatgc ttcatcgtcg gtccgggctg ccacgaccaa gtgacagcaa
     4381 tgctgtttca ctggttatgc ggcggatccg aaaagaaaac gttgatgccg gtgaacgtgc
     4441 aaaacaggct ctagcgttcg aacgcactga tttcgaccag gttcgttcac tcatggaaaa
     4501 tagcgatcgc tgccaggata tacgtaatct ggcatttctg gggattgctt ataacaccct
     4561 gttacgtata gccgaaattg ccaggatcag ggttaaagat atctcacgta ctgacggtgg
     4621 gagaatgtta atccatattg gcagaacgaa aacgctggtt agcaccgcag gtgtagagaa
     4681 ggcacttagc ctgggggtaa ctaaactggt cgagcgatgg atttccgtct ctggtgtagc
     4741 tgatgatccg aataactacc tgttttgccg ggtcagaaaa aatggtgttg ccgcgccatc
     4801 tgccaccagc cagctatcaa ctcgcgccct ggaagggatt tttgaagcaa ctcatcgatt
     4861 gatttacggc gctaaggatg actctggtca gagatacctg gcctggtctg gacacagtgc
     4921 ccgtgtcgga gccgcgcgag atatggcccg cgctggagtt tcaataccgg agatcatgca
     4981 agctggtggc tggaccaatg taaatattgt catgaactat atccgtaacc tggatagtga
     5041 aacaggggca atggtgcgcc tgctggaaga tggcgactaa gaattctgca gatatccagc
     5101 acagtggcag ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt
     5161 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     5221 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     5281 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     5341 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     5401 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     5461 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     5521 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     5581 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     5641 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     5701 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     5761 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     5821 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     5881 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     5941 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     6001 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     6061 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     6121 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     6181 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa
     6241 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     6301 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     6361 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     6421 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     6481 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     6541 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     6601 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     6661 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     6721 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     6781 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     6841 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     6901 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     6961 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     7021 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     7081 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     7141 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     7201 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     7261 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     7321 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
     7381 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     7441 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     7501 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     7561 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     7621 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     7681 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     7741 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     7801 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     7861 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     7921 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     7981 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     8041 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     8101 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     8161 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     8221 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     8281 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     8341 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     8401 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     8461 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     8521 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     8581 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     8641 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     8701 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     8761 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     8821 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     8881 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     8941 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     9001 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     9061 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     9121 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     9181 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     9241 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     9301 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     9361 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     9421 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     9481 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     9541 gaaaagtgcc acctgacgtc
//
