LOCUS       sequence-394564-         516 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for tagging PTP to the 5' end of PfPRMT5 gene by single crossover.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Lucky AB, Wang C, Liu M, Liang X, Min H, Fan Q, Siddiqui FA, Adapa 
            SR, Li X, Jiang RHY, Chen X, Cui L, Miao J
  TITLE     A type II protein arginine methyltransferase regulates merozoite 
            invasion in Plasmodium falciparum.
  JOURNAL   Commun Biol. 2023 Jun 22;6(1):659. doi: 10.1038/s42003-023-05038-z.
  PUBMED    37349497
REFERENCE   2  (bases 1 to 516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2023-06-22"; volume: "6(1):659. doi"; pages: " 
            10.1038/s42003-023-05038-z"
COMMENT     Sequence Label: pHD22y/PfPRMT5-PTP
FEATURES             Location/Qualifiers
     source          1..516
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..45
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             70..90
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             130..303
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             304..477
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNGAQAPK"
ORIGIN
        1 cctaggctgg aagatcaggt cgatcctcgt cttattgatg ggaaatatga tattccaact
       61 actgctagcg agaatttgta ttttcagggt gagctcaaaa ccgcggctct tgcgcaacac
      121 gatgaagccg tggacaacaa attcaacaaa gaacaacaaa acgcgttcta tgagatctta
      181 catttaccta acttaaacga agaacaacga aacgccttca tccaaagttt aaaagatgac
      241 ccaagccaaa gcgctaacct tttagcagaa gctaaaaagc taaatgatgc tcaggcgccg
      301 aaagtagaca acaaattcaa caaagaacaa caaaacgcgt tctatgagat cttacattta
      361 cctaacttaa acgaagaaca acgaaacgcc ttcatccaaa gtttaaaaga tgacccaagc
      421 caaagcgcta accttttagc agaagctaaa aagctaaatg gtgctcaggc gccgaaagta
      481 gacgcgaatt ccgcggggaa gtcaacctga ctcgag
//
